Os07g0124700 - Revision history

Xysj2019: /* Evolution */

2017-03-06T09:06:17Z

‎Evolution

Xysj2019: /* Expression */

2017-03-06T09:05:42Z

‎Expression

Xysj2019 at 09:05, 6 March 2017

2017-03-06T09:05:29Z

Xysj2012: /* Structured Information */

2015-06-12T08:31:50Z

‎Structured Information

Liyisong: /* References */

2014-06-09T13:43:48Z

‎References

Liyisong at 13:42, 9 June 2014

2014-06-09T13:42:15Z

Liyisong at 13:39, 9 June 2014

2014-06-09T13:39:34Z

Liyisong: /* Evolution */

2014-06-07T05:05:49Z

‎Evolution

Liyisong at 14:26, 2 June 2014

2014-06-02T14:26:03Z

Liyisong: /* Expression */

2014-06-02T04:58:12Z

‎Expression

@@ Line 17: / Line 17: @@
 * Comparison of the amino acid sequences of two AP2 domains and the conserved intervening linker region in CRL5, CRL5-like1 and CRL5-like2 from rice, and ANT, PLT1 and PLT2 from Arabidopsis.<ref name="ref1" />
 * CRL5 shares significant sequence similarity with AINTEGUMENTA (ANT) containing 2 AP2 domains in Arabidopsis.<ref name="ref4" /><ref name="ref2" />
-* Seven AINTEGUMENTA-like (AIL)/PLETHORA (PLT) proteins were reported to share high sequence similarity with ANT.<ref name="ref5" /><ref name="ref2" />
+* Seven AINTEGUMENTA-like (AIL)/PLETHORA (PLT) proteins were reported to share high sequence similarity with ANT.<ref name="ref5" /><ref name="ref2" /><ref name="ref3" />
 ===Expression===

@@ Line 20: / Line 20: @@
 ===Expression===
-* The expression of CRL5, which encodes a member of the large AP2/ERF transcription factor family protein, was observed in the stem region where crown root initiation occurs. Exogenous auxin treatment induced CRL5 expression without de novo protein biosynthesis, which also required the degradation of AUX/IAA proteins.
+* The expression of CRL5, which encodes a member of the large AP2/ERF transcription factor family protein, was observed in the stem region where crown root initiation occurs. Exogenous auxin treatment induced CRL5 expression without de novo protein biosynthesis, which also required the degradation of AUX/IAA proteins.<ref name="ref1" />
 ===Subcellular localization===

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os07g0124700''''' was reported as '''''CRL5''''' in 2011 <ref name="ref1" /> by researchers from Japan.
 ==Annotated Information==
 ===Function===
+* Cytokinin is known to have negative effects on de novo auxin-induced root formation. However, the regulatorymechanisms of root initiation by both cytokinin and auxin are poorly understood.
-. CRL5 encodes a member of the APETALA2 (AP2)/EHYLENE RESPONSIVE FACTOR (ERF) transcription factor family.<ref name="ref1" />
+* CRL5 encodes a member of the APETALA2 (AP2)/EHYLENE RESPONSIVE FACTOR (ERF) transcription factor family.<ref name="ref1" />
+* CRL5 functions downstream of the AUXIN (AUX)/INDOLE-3-ACETIC ACID (IAA) and AUXIN RESPONSE FACTOR (ARF)-mediated auxin signaling pathways involved in crown root initiation. <ref name="ref1" />
-. CRL5 functions downstream of the AUXIN (AUX)/INDOLE-3-ACETIC ACID (IAA) and AUXIN RESPONSE FACTOR (ARF)-mediated auxin signaling pathways involved in crown root initiation. <ref name="ref1" />
+* Further analysis revealed that CRL5 upregulates a type-A response regulator(RR) of cytokinin signaling, OsRR1. <ref name="ref1" />
+* It indicated that auxin-induced CRL5 functions as a positive regulator of crown root initiation through repression of cytokinin signaling.<ref name="ref1" />
-. Further analysis revealed that CRL5 upregulates a type-A response regulator(RR) of cytokinin signaling, OsRR1. <ref name="ref1" />
+* Schematic diagram of the de novo root initiation. CRL5 may function in de-differentiated cells (highlighted) to promote root initiation, not in the de-differentiation process. The factor inducing the de-differentiation is unknown(finger.g)<ref name="ref2" />
 ===Evolution===
-. Through a BLAST search using the amino acid sequence of CRL5, there are two homologous genes in rice, CRL5-like1 and CRL5-like2, which shared 57 and 49% amino acid identity,respectively, with CRL5.<ref name="ref1" />
+* Through a BLAST search using the amino acid sequence of CRL5, there are two homologous genes in rice, CRL5-like1 and CRL5-like2, which shared 57 and 49% amino acid identity,respectively, with CRL5.<ref name="ref1" />
+* Comparison of the amino acid sequences of two AP2 domains and the conserved intervening linker region in CRL5, CRL5-like1 and CRL5-like2 from rice, and ANT, PLT1 and PLT2 from Arabidopsis.<ref name="ref1" />
-[[File:treelyss.png]]
+* CRL5 shares significant sequence similarity with AINTEGUMENTA (ANT) containing 2 AP2 domains in Arabidopsis.<ref name="ref4" /><ref name="ref2" />
+* Seven AINTEGUMENTA-like (AIL)/PLETHORA (PLT) proteins were reported to share high sequence similarity with ANT.<ref name="ref5" /><ref name="ref2" />
 ===Expression===
-. CRL5 expression in various organs of wild-type rice as determined by semi-quantitative RT-PCR using ACT1 as a control(Figure 3a).CRL5 was expressed in nodes where crown roots were produced. High levels of CRL5 transcripts were observed in floral organs, but as differentiation progressed, CRL5 expression in floral organs was clearly downregulated (Figure 3a).<ref name="ref1" />
+* The expression of CRL5, which encodes a member of the large AP2/ERF transcription factor family protein, was observed in the stem region where crown root initiation occurs. Exogenous auxin treatment induced CRL5 expression without de novo protein biosynthesis, which also required the degradation of AUX/IAA proteins.
 <ref name="ref1" />
-. Comparison of a seminal root length between wild-type and crl5. *, Statistically significant difference from wild-type (P < 0.05)(Figure h).
+===Subcellular localization===
-[[File:hg.png|400px|]]
+* In situ hybridization with cross sections through the nodes in a 7-day-old wild-type plant. Localization of CRL5 in the regions where crown root initiation occurs (the identical region shown in fb and g), as determined using the antisense (i) and sense probes (j). No signal was detected with the sense probe of CRL5. The red arrowhead indicates the peripheral cylinder of vascular bundles. Scale bars = 200 lm.(Figure 3i,j).<ref name="ref1" />
-. It has been reported that a reduction of crown root number commonly occurs in auxin-related mutants in rice<ref name="ref6" /><ref name="ref7" /><ref name="ref8" /><ref name="ref9" />.An abnormality in gravitropic response is also a characteristic auxin-related phenotype in both rice and Arabidopsis<ref name="ref10" /><ref name="ref11" />.Thus,we examined the root gravitropic response in crl5 by measuring root curvature after gravistimulation at 90° for 24 h, followed by a return to vertical growth. In general, wild-type roots responded sharply to the change in the gravity vector, whereas the response of crl5 roots was clearly impaired. Comparing the root tip angles, all wild-type roots had root tip angles of over 35°(average 51.1± 2.6°), whereas all crl5 roots had angles of below 55° (average 31.1 ± 2.9°) (Figure 1i). The gravitropic response was therefore defective in crl5, which suggests that CRL5 has an auxin-related function.
+===Phenotypic analysis===
 ==Labs working on this gene==
-. Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan
+* Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan,
 ==References==

@@ Line 97: / Line 97: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 78: / Line 78: @@
 <ref name="ref5">Nole-Wilson S, Tranby TL, Krizek BA.AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or divisioncompetent
-states. Plant Mol Biol 2005; 57:613-28; PMID:15988559; DOI:10.1007/s11103-005-0955-6.
+states. Plant Mol Biol 2005; 57:613-28; PMID:15988559; DOI:10.1007/s11103-005-0955-6.</ref>
 <ref name="ref6">Inukai, Y., Sakamoto, T., Ueguchi-Tanaka, M., Shibata, Y., Gomi, K., Umemura, I., Hasegawa, Y., Ashikari, M., Kitano, H. and Matsuoka, M. (2005)
-Crown Rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin Signaling. Plant Cell, 17, 1387–1396.
+Crown Rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin Signaling. Plant Cell, 17, 1387–1396.</ref>
-<ref name="ref7">Liu, H., Wang, S., Yu, X., Yu, J., He, X., Zhang, S., Shou, H. and Wu, P. (2005) ARL1, a LOB domain protein required for adventitious root ormation in rice. Plant J. 43, 47–56.
+<ref name="ref7">Liu, H., Wang, S., Yu, X., Yu, J., He, X., Zhang, S., Shou, H. and Wu, P. (2005) ARL1, a LOB domain protein required for adventitious root ormation in rice. Plant J. 43, 47–56.</ref>
-<ref name="ref8">Kitomi, Y., Ogawa, A., Kitano, H. and Inukai, Y. (2008) CRL4 regulates crown root formation through auxin transport in rice. Plant Root, 2, 19–28.
+<ref name="ref8">Kitomi, Y., Ogawa, A., Kitano, H. and Inukai, Y. (2008) CRL4 regulates crown root formation through auxin transport in rice. Plant Root, 2, 19–28.</ref>
 <ref name="ref9">Liu, S., Wang, J., Wang, L., Wang, X., Xue, Y., Wu, P. and Shou, H. (2009) Adventitious root formation in rice requires OsGNOM1 and is mediated by
-the OsPINs family. Cell Res. 19, 1110–1119.
+the OsPINs family. Cell Res. 19, 1110–1119.</ref>
-<ref name="ref10">Leyser, H.M., Pickett, F.B., Dharmasiri, S. and Estelle, M. (1996) Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter. Plant J. 10, 403–413.
+<ref name="ref10">Leyser, H.M., Pickett, F.B., Dharmasiri, S. and Estelle, M. (1996) Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter. Plant J. 10, 403–413.</ref>
-<ref name="ref11">Tian, Q. and Reed, J.W. (1999) Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development, 126, 711–721.
+<ref name="ref11">Tian, Q. and Reed, J.W. (1999) Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development, 126, 711–721.</ref>
 </references>

@@ Line 19: / Line 19: @@
 . Through a BLAST search using the amino acid sequence of CRL5, there are two homologous genes in rice, CRL5-like1 and CRL5-like2, which shared 57 and 49% amino acid identity,respectively, with CRL5.<ref name="ref1" />
-[[File:tree.png]]
+[[File:treelyss.png]]
 . Comparison of the amino acid sequences of two AP2 domains and the conserved intervening linker region in CRL5, CRL5-like1 and CRL5-like2 from rice, and ANT, PLT1 and PLT2 from Arabidopsis.<ref name="ref1" />

@@ Line 24: / Line 24: @@
 [[File:d.png]]
 ===Expression===
@@ Line 68: / Line 72: @@
 <ref name="ref3"> Inukai Y, Sakamoto T, Ueguchi-Tanaka M, et al. Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling[J]. The Plant Cell Online, 2005, 17(5): 1387-1396.</ref>
 </references>