Os07g0182900 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T08:33:57Z

‎Structured Information

Ibpchenwenbo: /* References */

2014-06-06T14:27:11Z

‎References

Ibpchenwenbo: /* Labs working on this gene */

2014-06-06T14:19:42Z

‎Labs working on this gene

Lvying: /* Expression */

2014-06-06T14:02:54Z

‎Expression

Lvying: /* References */

2014-06-06T14:01:48Z

‎References

Lvying: /* Knowledge extension */

2014-06-06T13:58:38Z

‎Knowledge extension

Lvying: /* Evolution */

2014-06-06T13:57:23Z

‎Evolution

Lvying: /* Expression */

2014-06-06T13:56:43Z

‎Expression

Lvying: /* Mutation assay */

2014-06-06T13:56:07Z

‎Mutation assay

Lvying: /* Sequence comparison between OsMET1a and OsMET1b */

2014-06-06T13:55:38Z

‎Sequence comparison between OsMET1a and OsMET1b

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ==References==
 <references>
 <ref name="ref1">Takaki Yamauchi et al. The MET1b gene encoding a maintenance DNA methyltransferase

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 * Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology (NAIST), Ikoma, Japan
 * Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo, Japan
-* Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies
+* Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Okazaki, Japan
 ==References==

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 ===Expression===
-* Ribonuclease protection assays showed that MET1a and MET1b both express in highly replicating and dividing cells;<ref name="ref2" />
+* Ribonuclease protection assays showed that MET1a and MET1b both express in highly replicating and dividing cells;
-* MET1b is more abundantly expressed than MET1a in all of the tissues examined, like callus, root and inflorescence;<ref name="ref2" />
+* MET1b is more abundantly expressed than MET1a in all of the tissues examined, like callus, root and inflorescence;
 * OsMET1b does not express in differentiated tissue (10-day-old leaf), and no expression for either gene was found in mature leaves.<ref name="ref2" />

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 ==References==
 Please input cited references here.
 ==Structured Information==

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 ===Knowledge extension===
-Apart from the main information listed above about function, expression and evolution about OsMET1 gene, there are also some advanced researches about this gene being conducted in the past few years. Takaki Yamauchi et al. reported in 2007 that the alternative splicing mechanisms of OsMET1a transcript and OsMET1b transcript differ, which is key to the regulation of active OsMET1 protein generation in different tissues. Daisuke Miki et al. reported in 2008 that de novo DNA methylation can be induced by siRNA targeted to endogenous transcribed sequences which is gene-specific and OsMET1-indepent in rice, which is also the first time to observe transcriptional gene silencing by siRNAs. Takaki Yamauchi et al. reported in 2009 that they successfully realized homologous recombination-promoted knock-in targeting to monitor gene expression by fusing a reporter gene named GUS codingβ-glucuronidase with its promoter in rice, in principle, this method can be applied to modify any endogenous gene which would promote basic and applied plant research.
+Apart from the main information listed above about function, expression and evolution about OsMET1 gene<ref name="ref1" /><ref name="ref2" />, there are also some advanced researches about this gene being conducted in the past few years<ref name="ref3" /><ref name="ref4" /><ref name="ref5" />. Takaki Yamauchi et al. reported in 2007 that the alternative splicing mechanisms of OsMET1a transcript and OsMET1b transcript differ, which is key to the regulation of active OsMET1 protein generation in different tissues<ref name="ref3" />. Daisuke Miki et al. reported in 2008 that de novo DNA methylation can be induced by siRNA targeted to endogenous transcribed sequences which is gene-specific and OsMET1-indepent in rice, which is also the first time to observe transcriptional gene silencing by siRNAs<ref name="ref4" />. Takaki Yamauchi et al. reported in 2009 that they successfully realized homologous recombination-promoted knock-in targeting to monitor gene expression by fusing a reporter gene named GUS codingβ-glucuronidase with its promoter in rice, in principle, this method can be applied to modify any endogenous gene which would promote basic and applied plant research<ref name="ref5" />.
 ==Labs working on this gene==

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 ===Evolution===
-This phylogenetic relationships between eukaryotic and prokaryotic DNA methytransferase were derived using the AlignX program of the VectorNTI suite, suggesting that the rice DNA methytransferase are related to DNA methytransferase of Dnmt1/MET1 class in diverse organisms from several kingdom.
+This phylogenetic relationships between eukaryotic and prokaryotic DNA methytransferase were derived using the AlignX program of the VectorNTI suite, suggesting that the rice DNA methytransferase are related to DNA methytransferase of Dnmt1/MET1 class in diverse organisms from several kingdom.<ref name="ref2" />
 [[File:112233cwb.jpeg]]
 Figure 3. Phylogenetic relationships between eukaryotic and prokaryotic DNA methytransferase
-Phylogenetic relationships between eukaryotic and prokaryotic MTases were derived using the AlignX program of the VectorNTI suite with the following GenBank accessions: maize MET1 (AF063403), rice MET1-1 (OsMET1-1, AF462029), rice MET1-2 (OsMET1-2, TPA BK001405), Arabidopsis MET1(AtMET1, AT5G49160) and MET2 (MET2-type C-5 DNA MTase, AT4G08990), pea MET1 (putative C-5 DNA MTase, AF034419), carrot MET1 (carrot C-5 DNA MTase, AF007807) and MET2 (MET2-type C-5 DNA MTase, AF007808), tobacco MET1 (NtMET1, AB030726), tomato (putative C-5 DNA MTase AJ002140), rat DNMT1 (AB012214), human DNMT1 (AF180682), Xenopus DNMT1(D78638), zebra fish DNMT1 (AF483203), chicken DNMT1 (2112268A), Neurospora DIM2 (AF348971), Ascobolus DNMTase (Z96933), rice MET2a (AC069324), maize MET2a (AF243043) and Haemophilus haemolyticus M.HhaI (XYH1H1)
+Phylogenetic relationships between eukaryotic and prokaryotic MTases were derived using the AlignX program of the VectorNTI suite with the following GenBank accessions: maize MET1 (AF063403), rice MET1-1 (OsMET1-1, AF462029), rice MET1-2 (OsMET1-2, TPA BK001405), Arabidopsis MET1(AtMET1, AT5G49160) and MET2 (MET2-type C-5 DNA MTase, AT4G08990), pea MET1 (putative C-5 DNA MTase, AF034419), carrot MET1 (carrot C-5 DNA MTase, AF007807) and MET2 (MET2-type C-5 DNA MTase, AF007808), tobacco MET1 (NtMET1, AB030726), tomato (putative C-5 DNA MTase AJ002140), rat DNMT1 (AB012214), human DNMT1 (AF180682), Xenopus DNMT1(D78638), zebra fish DNMT1 (AF483203), chicken DNMT1 (2112268A), Neurospora DIM2 (AF348971), Ascobolus DNMTase (Z96933), rice MET2a (AC069324), maize MET2a (AF243043) and Haemophilus haemolyticus M.HhaI (XYH1H1)<ref name="ref2" />
 ===Knowledge extension===

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 ===Mutation assay===
-* RNAi-mediated  knockdown  analysis of the rice MET1 genes showed that it resulted in reactivation of a silenced β-glucuronidase (GUS) transgene in rice callus.
+* RNAi-mediated  knockdown  analysis of the rice MET1 genes showed that it resulted in reactivation of a silenced β-glucuronidase (GUS) transgene in rice callus.<ref name="ref1" />
-* A Osmet1a null mutant has no obvious phenotypes, while Osmet1b null mutant exhibited abnormal seed phenotypes, such as early embryonic lethality, decreased levels of DNA methylation at repetitive CentO sequences and at the FIEI gene locus in the embryos.
+* A Osmet1a null mutant has no obvious phenotypes, while Osmet1b null mutant exhibited abnormal seed phenotypes, such as early embryonic lethality, decreased levels of DNA methylation at repetitive CentO sequences and at the FIEI gene locus in the embryos.<ref name="ref1" />
 [[File:Figure 1.Phenotyes of normal type seeds and OsMET1b null mutant type(A to C) seeds.png]]
-Figure 2.Phenotyes of normal type seeds and OsMET1b null mutant type(A to C) seeds
+Figure 2.Phenotyes of normal type seeds and OsMET1b null mutant type(A to C) seeds<ref name="ref1" />
 ===Expression===

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 [[File:112233.JPEG]]
-Figure 1:Proportionate diagram of OsMET1-1 showing conserved domains: S-stretch serine-rich region; E-rich region glutamic acid-rich region; BAH bromo adjacent homology domain (black boxes with white dots); IQ motif (calmodulin binding) isoleucine (I) and glutamine (Q) motif (hatched box); NLS putative nuclear localization signal (oval); KG linker the lysine-glycine repeat, KNKGKG (gray box); I to X the catalytic domain with eight highly conserved DNA MTase motifs (black bars).
+Figure 1:Proportionate diagram of OsMET1-1 showing conserved domains: S-stretch serine-rich region; E-rich region glutamic acid-rich region; BAH bromo adjacent homology domain (black boxes with white dots); IQ motif (calmodulin binding) isoleucine (I) and glutamine (Q) motif (hatched box); NLS putative nuclear localization signal (oval); KG linker the lysine-glycine repeat, KNKGKG (gray box); I to X the catalytic domain with eight highly conserved DNA MTase motifs (black bars).<ref name="ref2" />
 ===Mutation assay===