Os07g0257200 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T08:38:06Z

‎Structured Information

Xysj2012 at 15:01, 13 August 2013

2013-08-13T15:01:14Z

Xysj2012 at 08:57, 13 August 2013

2013-08-13T08:57:10Z

Xysj2012: /* Wild Type & Mutations */

2013-08-13T08:54:01Z

‎Wild Type & Mutations

Xysj2012: /* Wild Type & Mutations */

2013-08-13T08:52:22Z

‎Wild Type & Mutations

Xysj2012: /* Function */

2013-08-13T08:51:20Z

‎Function

Xysj2012: /* Function */

2013-08-13T08:50:46Z

‎Function

Xysj2012: /* References */

2013-08-13T08:49:11Z

‎References

Xysj2012: /* References */

2013-08-13T08:48:57Z

‎References

Xysj2012: /* References */

2013-08-13T08:48:34Z

‎References

@@ Line 48: / Line 48: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 17: / Line 17: @@
 * The expression analysis by ''Yasuhiro Ishimaru'' et al. through ''GUS'' staining, further showed that ''OsNRAMP5'' expression was mainly expressed in roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem(Figure 2)<ref name="ref1" />. <br>
 * They also found that the expression level of ''OsNRAMP5'' decreased in root and and shoots when exposed to Cd while the expression of ''OsNRAMP1'' increased in both tissues. No significant change was found in the expression level  of other ''OsNRAMP'' family genes.<br>
-* The Subcellular Localization analysis by ''Akimasa Sasaki'' et al. and ''Yasuhiro Ishimaru'' et al. both indicated that ''OsNRAMP5'' is localized to the plasma membrane. <br><br>
+* The Subcellular Localization analysis by ''Akimasa Sasaki'' et al. and ''Yasuhiro Ishimaru'' et al. both indicated that ''OsNRAMP5'' is localized to the plasma membrane. <br><br><br>
 ===Evolution===

@@ Line 77: / Line 77: @@
 AA = <aaseq>MEIERESSERGSISWRASAAHDQDAKKLDADDQLLMKEPAWKRF                     LAHVGPGFMVSLAYLDPGNLETDLQAGANHRYELLWVILIGLIFALIIQSLAANLGVV                     TGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIPEVIGTAFAFNILFHIPVWVGVLI                     TGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGELSIVKPPAKEVMKGLFIPRLN                     GDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRGIKDGCRFFLYESGFALFVA                     LLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLKNVLGKSSAIVYGVALLA                     SGQSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSLIVSIIGGSRGAGRLI                     IIASMILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLGLLIIGINMYFLST                     SFVGWLIHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTFVADSSLAAVVD                     AEKADAGDLAVDDDEPLPYRDDLADIPLPR</aaseq>|
 DNA = <dnaseqindica>6638..6970#5533..5628#4806..4937#3715..3819#3260..3433#2900..2969#2619..2800#2285..2394#1181..1285#1020..1098#681..721#474..552#97..207#gtcactaccaccattctcttcttcgtctacttccagctagcctgagctctagcttagctctagcttagcctgaagaagctaagagaggaagcaacaatggagattgagagagagagcagtgagagagggagcatcagctggagagctagtgcggcacatgatcaagatgccaagaagctcgacgcagatgatcagctgctaatgaaggtcagttgctaccttaattatcacaaagttgtcttcttgtaagcactccactgtcaccatggacagttgttcactgtaactgcagaagcaagagcagagcttaatgtagatagatcttgagagtacttcctggcatggcattcattgctcatgaatgttcatgtgtgtgtgtgtatgtggtaattaaggactgatggaacattggatcctcaattatgtctgtgtttctttttgttagtttctcagttttggtaaattgtgtacaggagcctgcatggaaaaggttccttgcccatgttggtcctggattcatggtgtctttagcctacttggatcctggcaattgtgagaatctctctctctctctctctctctctctctctctctctctctctctctctctctctcaactgaaacttttattttgacaaactctggattgtaactgttaattttgtcacaaaatgaaacagtggaaaccgatctgcaagccggagccaaccacagatatgaggtacgtacagtcgactttattttactgtttacgcactgaatgactgaaagcatgagtaactttcatgctttctaggtgttcgtccctttccttgtccctcgcagactcacactccattaattcatctgctagctccatcaaattctccaaattgacaaacttttcagaaccacttttaatccccagtgcatatgtatctcactctcacatcatctagagaaaagagaaaagtgaataatctgcaccattaattgatttctatgtgcttttctgtccattgtgttgtttcaatttgcagctgctctgggtgattctgattggactcatcttcgcacttatcatacagtcgctagcagctaatcttggagtggttacaggtgactaaatgacacgcaattaaactgtaattaccaacttgataaagtgctaatatatggccggcattaatttacatcctagggaggcatctggctgagatctgcaagagtgagtaccccaagttcgtcaagattttcctatggctgctggcagagttggccgtcatcgctgcagatatcccagaaggtatatacttattagtaacaacttgacacggtccagcctgaataattaatttattaaactacttgtgtttaacagtgtcaccatctttgactttgagctaattaattaattcagtttaagctctctgacagtagaagaaaacaagttgatctggctagcatatgtagctccaaccgtacgggcctacatactggaattcctaatccacaaacataggcaaattaatatctcaccaatcaccatcaagccacgttcttctcagctactcaccagattattctcacatattatttctttcatgtctctagctagtaaatatgtttttctaaaaaaaatatattctgcgatattgcatagaatacaagttctaagccattttttaaaaaaatatttaattcatccatataataaggtagataatatgtaccaataatatatctgttgaataatctgtttcaaacataagcaaatgcgcgttttagctgtttagaggagtgaccacgtccacagtagcttcccataaaagtgtcaatgatgcacatcaagattagattttaaacactgggtgagtacgttcatcatcttacgcagattagtaaacaactattttgtcaccctcttgtcatttcacatttgtcctgtcctgtatacatccatgtgtgtttaaacaataacaacaatatctaaatatctatctatctagcaacatggaagagagatcagtggtgtagcagttgcaaaacggagctgcgcgaagctttgctgatcgggattagttaacctcaaccgttgtaaattgcctttttaattgatcatgcatgcaagttcttttttaaaaaaattaaactagttatggaagatacctcacatgatgatagcaaaggtgatatatagatagatagatagatcgatcaacagatagaaaggtacagatcgatgtgattataattaattaattaattacatgtcgtttgatgttgttaattttggtggatgcagttatagggacggcctttgctttcaacatattgttccatattccggtgtgggtcggcgtcctcatcaccggcaccagcactctactgcttcttggcctccaaaaatacggggtataaattattttttgtaagttgtttttgtttagtatcacaagcattagcgtaggatgcactcaaagaaacgattataaaaatggaatagtatatgaaattagctagttggatcgattgtcaagtactcaaatggcatattatatgtacattattcagttttttttctctgactgtggaactgcatgaaaattgattgatgaattttttttgaaaaaaaacaggtgaggaagctggagtttctgatatcgatgctggtgttcgtgatggcggcgtgcttcttcggggagctgagcatcgtgaagccgccggcgaaggaggtgatgaaggggctcttcatccccaggctcaacggcgacggcgccaccgccgacgccattgccctcctcggagctcttgtcatgccgtacgcttttacatacatacatacatacgcaacaatcaatcaattaattcatacaactaatcagttaattaactactgcatgcatttatatatatgcagccacaatctgttcttgcattctgccttggtgctatcgaggaagacaccggcatcagtcagaggaatcaaggtagctatattcagcctgggcagatcgagataggtatagggtagctaggacatgaactcgatctagtcctgtctcttaaatcttgtttaaattttattaaattaacatgtaaatttcatagaaaaccaatactttattagctaagttttattttagcactaatcttctataatttcgggctccagtaccactggattcagcttagaattttttcatgcatggaaagaaaccgaacaaagatccaggctaataattaatatgtactatatggtgtgtgaattgccacgtaggacgggtgcaggttcttcctgtacgagagcgggttcgcgctgttcgtggcgctgctgataaacatcgccgtcgtctccgtctccggcaccgcctgctcctccgccaacctctcccaagaggacgccgacaagtgcgccaacctcagcctcgacacctcctccttccttctcaaggtcattcattcattattacttcctactccttaatattaaaaatttttaaatggattagacgtatcctagtactacagatagaaatctattcagattcgtagtactagcttgtatgtgttcaatttaatcaatatttaattaaccacaataataccgaattagtctacacgatttctccgtcctaaaatataataactttattagatatgtttatatttttgaataggaggagtaactaatttatggtcaaattgtgacgtcaatttttttatgtggttcagaacgtgctgggcaagtcgagtgcgatcgtgtacggcgtggcactgttggcatctgggcagagctccactattaccggcacatacgctggacagtacatcatgcaggtaattaattaaattaattaacttaactgtacctgtacctgtacatcaccagctagcttagcgcgtaattaaatgattgaactataattaattaaacggctagctgccacataattaatttgttaatccgttcaattatggaggttaactgacgaattgtaggggtgcacacctaatcctgtttacatcacgaatatttagtgatataatattcctttattaattaggtaatctggcgatcctcgtcggatttcaccatatgtttttgttaaagtcagatcgaattaagacgctaccttgctgccactacttaccactttcgtccatatttctccgctcttttggtctagtccatttcacggtgcagtctcatcaataaccagcgattttactgtttacatatacttagaaaaccaattgattaataaaaatatgctaacgaaactctataaattatattaaaaaaaataggatatcccatcgcaccgtaaaattttctttctgaagtgaacgttgagaaagagaacaactagttttcctaatctacctggcgggctaaccgatctagttcgagtctcattcctcttaataatctatttatgatatgaagcgtatttcagttacgggataaatcggacccgaaaatatatatttattttttcactccaaaggactgatcttcaggatatactcactccgtctctaaatatttgacgtcgttgactttttataaatatgtttgaccgttcgtcttatgcaaaaacttttatgaatattatttaaaaaactagagtagtagtagtatatacaacaatgaatcaaatgataagaaaagaattaataactacttaaatttattgaataagatgaatggttaaacatgtttagaaaaatcaacggcgtcaaatatttaatgacggaaggagtatatgataaatcatcatcatgtcgcgttgatatatatgatatgatccatgtatgtgcagggtttcttggacatcaggatgaggaagtggcttcggaacctgatgacaagaaccatcgccatcgcgccgagcctcatcgtctccatcatcggcggctccaggggcgccggccgcctcatcatcatcgcttcggtcagcatttttattattactatttactatagcaaatctttactattattactattttactatagcaaactgcgtgcgccatacggcggcgcccgccggcgagcaaccggccggccgtcgtgtcgccgatcgattaaaatttaatgcgtttaaatttttaattttgcacatgcccaaacagtcacgtgtgtgcagtcgatcgtgcggatttatgtagctagctaggtagtagtggggtatccactaggtacctagtgacatagtactaagagcatatatatatgtcattagagcaaaagcactttttcttctttttttagattattgccaaatcaaagtagtgatctgattagcaaaatgtcactgactagctaggtatcttttctttttttgcaatctgatctaaaatagcaccatgcatatatataatacagtgtaccatctagctagactagcttccaactcaataagtaaggactaattatatataattaaatcaaatcaaagtagtgatctgatcattgctagttcttgtttgcttaattacatatatatgattaattgttcctaatccgtacgtgttgtgtgcatgcagatgatactgtccttcgagctgccgtttgctctcatccctcttctcaagttcagcagcagtaagagcaagatggggccccacaagaactctatctatgtaagatcgtacactatatatatgcattgcttaattatagtttgtttcttaatctcaattggcagcaaatgtacaagtaacataaatatatgcatctatctgtccatgcatgaaattagagtttcaataattgttggtaaagaatataaattaatgatgatagttggctagctagctggtccttcaccctgcatgcatgcaacccctacaggacaccatgttctataggtcagtgtgtgcataatatagatcgagatgtgcagtgaattgaattgaatcattagctacctagctcatgcaatagtattatagcacagcaggggccaatgcaggacaggtatatatatggtccagccatgcatatagaggatcgtcgtacatatgaaaacgacgaccaagatgtacgtcctgcatcatttttctgtcttgcagtagtaccagagattaatcatgtattgcgtcgtcggcatgatgtgtcaaaatcatgcagtaacgtacttgcttgatctttacagacatcgaagagccaagttcttcagtacactatatatattttttcttgcatggaagtaaaatgtaaatataatttaacatagatcattacgtatgtcgttgtatctctacttaaacatttctagacacatacaagtcaacattagaaaatgtaattcttttttaaggaccataaataaactgatctacaacaaaattgaacaaatgtaaatttatttcttattattacacacatggctatatatatatatatatatgtgtgtgtatatatatatatatgtgtatatatatatatgtgtatatatatatatatatatatatgtatatatatatatatatactacctataaaaattgcgcatgctcatcagtcgatacccgttaaaaagatatataagttaccacccgtcaaagatgactggttatttagtgacgctatcaatgaattaattagcgtcatgtatgtcaaaaaataaattgaattgaattgaatgcagataatagtgttctcgtggttcctgggtctgctcatcatcggcatcaacatgtacttcctgagcacgagcttcgtcggctggctcatccacaacgacctccccaagtacgccaacgtgctcgtcggcgccgccgtcttcccgttcatgctcgtctacatcgtcgccgtcgtctacctcaccatcaggaaggactccgtcgtcaccttcgtcgccgactcctccctcgccgccgtcgtcgacgccgagaaggccgacgccggcgacctcgccgtcgacgacgacgagcccttgccgtaccgcgacgacctggccgacatcccgctcccaaggtagagaagaagaagatcgacatgcatacgtatggtatagcgtatgtatacgtacgtatatacgcgtacattgtgaatatacgtacttacgtacgtatatgtgcatcctctatggatgccacgtgcgtgcgtcggagccgttcgtttatatttgtgcggtccagatcgctcgcagatcgatcgatcgacaagggtgtgcaattaatatataagcatttgaggggaggtggctcatgtatccttgtaatttatatgtacgatatgatttatcataattcagggcagagccaacgtcct</dnaseqindica>|
-Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065847.1 RefSeq:Os07g0257200]|
+Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065847.1 RefSeq:Os07g0257200],[http://www.ricedata.cn/gene/list/2058.htm China Rice Data Center] |
 }}
 [[Category:Genes]]

@@ Line 8: / Line 8: @@
 ===Wild Type & Mutations===
-* The ''Nramp5'' Knock-out Line reported by ''Akimasa Sasaki'' in 2012 showed that the growth of both the mutant shoots and roots was less than that of wild-type rice without any heavy metal ions treatments<ref name="ref2" />. And severe chlorotic symptoms could be found in the leaves of the ''Nramp5'' knock-out line. <br>
+* The ''OsNramp5'' Knock-out Line reported by ''Akimasa Sasaki'' in 2012 showed that the growth of both the mutant shoots and roots was less than that of wild-type rice without any heavy metal ions treatments<ref name="ref2" />. And severe chlorotic symptoms could be found in the leaves of the ''OsNramp5'' knock-out line. <br>
 * When treated with heavy metal ions, no significant difference was observed in the concentration of Zn and Cu for the roots and shoots between the mutants and wild-type line. While both the concentration of Mn and Cd in the mutant roots and shoots was remarkably lower than in the wild-type line (Figure 1)<ref name="ref2" />.

@@ Line 8: / Line 8: @@
 ===Wild Type & Mutations===
-* The ''Nramp5'' Knockout Line reported by ''Akimasa Sasaki'' in 2012 showed that the growth of both the mutant shoots and roots was less than that of wild-type rice without any heavy metal ions treatments<ref name="ref2" />. And severe chlorotic symptoms could be found in the leaves of the ''Nramp5'' knockout line. <br>
+* The ''Nramp5'' Knock-out Line reported by ''Akimasa Sasaki'' in 2012 showed that the growth of both the mutant shoots and roots was less than that of wild-type rice without any heavy metal ions treatments<ref name="ref2" />. And severe chlorotic symptoms could be found in the leaves of the ''Nramp5'' knock-out line. <br>
 * When treated with heavy metal ions, no significant difference was observed in the concentration of Zn and Cu for the roots and shoots between the mutants and wild-type line. While both the concentration of Mn and Cd in the mutant roots and shoots was remarkably lower than in the wild-type line (Figure 1)<ref name="ref2" />.

@@ Line 3: / Line 3: @@
 [[File:NRAMP5_1.jpg|right|thumb|300px|'''Figure 1.''' ''Wild-type lines VS. NRAMP5 knock-out lines (from reference<ref name="ref2" />).'']]
 ===Function===
-* The plant ''Natural resistance associated macrophage protein'' (NRAMP) gene family play an important role in the transportation of metal ions and maintaining their intracellular homeostasis<ref name="ref4" />. As a member of rice NRAMP gene family, ''OsNRAMP5'' is a major transporter responsible for Mn uptake in the roots. <br>
+* The plant ''Natural resistance associated macrophage protein'' (NRAMP) gene family play an important role in the transportation of metal ions and maintaining their intracellular homeostasis<ref name="ref4" />. As a member of rice NRAMP gene family, ''OsNRAMP5'' is a major transporter responsible for Mn uptake and Cd in the roots. <br>
 * It can participate in the major route for Cd entering the root cells from the external environment in rice<ref name="ref1" /><ref name="ref2" />. The research by ''Yasuhiro Ishimaru'' also pointed out that ''OsNRAMP5'' would function as a Fe and Mn transporter during flowering and seed development<ref name="ref3" />.

@@ Line 41: / Line 41: @@
 Ishimaru Y, Bashir K, Nakanishi H, et al. OsNRAMP5, a major player for constitutive iron and manganese uptake in rice[J]. Plant signaling & behavior, 2012, 7(7): 763-766.
 </ref>
 ==Structured Information==

@@ Line 37: / Line 37: @@
 * <ref name="ref2">
 Sasaki A, Yamaji N, Yokosho K, et al. Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice[J]. The Plant Cell Online, 2012, 24(5): 2155-2167.
 </ref>

@@ Line 34: / Line 34: @@
 * <ref name="ref1">
 Ishimaru Y, Takahashi R, Bashir K, et al. Characterizing the role of rice NRAMP5 in manganese, iron and cadmium transport[J]. Scientific reports, 2012, 2.
 </ref>