Os07g0471000 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T08:39:48Z

‎Structured Information

2014-05-21T05:42:05Z

‎Structure

2014-05-21T05:41:47Z

‎Expression

2014-05-21T05:41:10Z

‎Structure

2014-05-21T05:40:17Z

‎Expression

2014-05-21T05:33:14Z

‎Expression

2014-05-21T05:32:37Z

‎Expression

2014-05-21T05:31:28Z

‎Expression

2014-05-21T05:11:42Z

‎Expression

2014-05-21T05:11:17Z

‎Expression

@@ Line 71: / Line 71: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 41: / Line 41: @@
 (D and E) Dimer interface interactions involving conserved residues in the kinase domain (D) and
 the KEN domain (E), respectively, viewed along the two fold axis of rotational symmetry. For clarity, only residues targeted for mutation in this study are shown in ball and stick representation. Residues colored yellow and white originate from promoter 1 and 2, respectively. Ribbons coloring scheme corresponds to that in (A).
 [[File:3.jpg]]

@@ Line 29: / Line 29: @@
 [[File:o.jpg|right|thumb|250px|Figure 1 Pgenotypes of wild-type and transgenic rice. (A) Plant phenotype of the wild-type (wt) and IRE1-OE, IRE1-KD, K519A-OE and K833A-OE rice lines (2 months after germination)(From reference <ref name="ref5" />).'']]
-[[File:2.jpg| Figure 2 Schematic representation of putative domains in OsIre1p predicted from hydropathy plot and database search (From reference <ref name="ref6" />).'']]
+[[File:2.jpg]]
 ===Gene construction===

@@ Line 40: / Line 40: @@
 (C) Detailed stereoview of the KEN domain dimer as shown in (A). Disordered regions corresponding to putative RNA specificity determinants are shown as red dashed lines.
 (D and E) Dimer interface interactions involving conserved residues in the kinase domain (D) and
-the KEN domain (E), respectively, viewed along the two fold axis of rotational symmetry. For clarity, only residues targeted for mutation in this study are shown in ball and stick representation. Residues colored yellow and white originate from promoter 1 and 2, respectively. Ribbons coloring scheme corresponds to that in (A).[[File:3.jpg]]
+the KEN domain (E), respectively, viewed along the two fold axis of rotational symmetry. For clarity, only residues targeted for mutation in this study are shown in ball and stick representation. Residues colored yellow and white originate from promoter 1 and 2, respectively. Ribbons coloring scheme corresponds to that in (A).
 ===Evolution===

@@ Line 29: / Line 29: @@
 [[File:o.jpg|right|thumb|250px|Figure 1 Pgenotypes of wild-type and transgenic rice. (A) Plant phenotype of the wild-type (wt) and IRE1-OE, IRE1-KD, K519A-OE and K833A-OE rice lines (2 months after germination)(From reference <ref name="ref5" />).'']]
-[[File:2.jpg]]
+[[File:2.jpg| Figure 2 Schematic representation of putative domains in OsIre1p predicted from hydropathy plot and database search (From reference <ref name="ref6" />).'']]
 ===Gene construction===

@@ Line 28: / Line 28: @@
 The results suggest that the overexpression of K519A and K833A has an inhibitory influence on the '''OsIRE1'''/OsbZIP50 signaling and ER stress pathway. However, the mechanism of why the overexpression of K519A and K833A has an inhibitory influence on the OsIRE1/OsbZIP50 signaling and ER stress pathway isn’t clear <ref name="ref5" />.
 [[File:o.jpg|right|thumb|250px|Figure 1 Pgenotypes of wild-type and transgenic rice. (A) Plant phenotype of the wild-type (wt) and IRE1-OE, IRE1-KD, K519A-OE and K833A-OE rice lines (2 months after germination)(From reference <ref name="ref5" />).'']]
-  [[File:over.jpg]]  [[File:2.jpg]]
 ===Gene construction===

@@ Line 21: / Line 21: @@
 | | 5’- ATGCATCACATACACGGCCGTTG-3’
 |}
 Shimpei Hayashi ''et al.''(2012) generate the transgenic plants of OsbZIP50 (OsbZIP50 KD) or rice '''IRE1''' ('''OsIRE1''') by RNA interference. The OsIRE1 knockdown (KD) lines showed severe defects in growth <ref name="ref4" />.

@@ Line 7: / Line 7: @@
 ===Expression===
 By using the full-length atpI protein sequence (NCBI Reference Sequence: NP_001059606.1), the primers is well-built, which could conduct gene amplication and gene analysis for further research.
 [[File:primer.jpg]]