Os07g0592600 - Revision history

Xysj1980: /* Labs working on this gene */

2017-03-08T01:46:49Z

‎Labs working on this gene

Xysj1980 at 01:46, 8 March 2017

2017-03-08T01:46:38Z

Xysj1980 at 01:45, 8 March 2017

2017-03-08T01:45:22Z

Xysj2012: /* Structured Information */

2015-06-12T08:46:55Z

‎Structured Information

Ct at 06:09, 6 June 2014

2014-06-06T06:09:13Z

Zhdjsh: /* Labs working on this gene */

2014-06-05T13:16:22Z

‎Labs working on this gene

Zhdjsh: /* Labs working on this gene */

2014-06-05T13:16:09Z

‎Labs working on this gene

Zhdjsh: /* Evolution */

2014-06-05T13:13:17Z

‎Evolution

Zhdjsh: /* Evolution */

2014-06-05T12:47:26Z

‎Evolution

Zhdjsh: /* Evolution */

2014-06-05T12:46:31Z

‎Evolution

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 ==Labs working on this gene==
-* National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural
+* National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
 ==References==

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 ==Annotated Information==
 ===Function===
 * The rice '''''GH3-8''''' is an auxin-responsive gene functioning in auxin-dependent development, activates disease resistance in a salicylic acid signaling– and jasmonic acid signaling–independent pathway.

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-Please input one-sentence summary here.
+The rice '''''Os07g0592600''''' was reported as '''''GH3-8''''' in 2008 <ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Function===
-OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity<ref name="ref1" /><ref name="ref2" />
+===Phenotypic analysis===
-Auxin plays a central role in nearly all aspects of plant growth and development. The final molecular and cellular outcome of auxin signaling in a specific tissue/spatially localized domain depends very critically on the level of its biologically active pool. This is mostly regulated by a homeostasis between its biosynthesis and inactivation. The significance of auxin homeostasis is underscored by the finding that in Arabidopsis_99% of the IAA pool is maintained in the conjugated inactive form generated by various GH3 proteins, and only 1% of IAA occurs as the biologically active form<ref name="ref3" />. Thus, regulated expression of GH3 factors can contribute to auxin signaling and these genes are ideal targets for transcription regulators to execute their developmental functions.
+* Overexpression of '''''GH3-8''''' results in enhanced disease resistance to the rice pathogen Xanthomonas oryzae pv oryzae. * This resistance is independent of jasmonic acid and salicylic acid signaling. Overexpression of GH3-8 also causes abnormal plant morphology and retarded growth and development.
+* Both enhanced resistance and abnormal development may be caused by inhibition of the expression of expansins via suppressed auxin signaling.
 ===Expression===
-Rice GH3 genes are expressed at varying levels in various wild-type tissues and organs, with the transcript levels for OsGH3-1, 2, 3, 4, 5, 7, 8 and 11 being the highest in flowers (Jain et al. 2006a). The expression of six GH3 genes is de-regulated in OsMADS1 mutant panicles as seen in our analysis of transcript levels using whole-genome microarrays (Prasad et al. 2005, S. R. Yadav, I. Khanday and U. Vijayraghavan, unpublished data). OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity (Takase et al. 2004, Staswick et al. 2005). OsMGH3/OsGH3-8 (hence forth referred to as OsMGH3) is one amongst this family of genes whose expression is reduced in dsRNAi (double-stranded RNA interference) OsMADS1 panicles and in osmads6 mutant flowers (Prasad et al. 2005, Zhang et al. 2010).The expression domain of OsMGH3 overlaps with that of OsMADS1 at early stages of floret development (figure 1). In florets at later stages of development with differentiating organs, its expression overlaps more with OsMADS6 <ref name="ref4" /><ref name="ref5" /><ref name="ref6" />.
+* '''''GH3-8''''' Expression Is Regulated by Multiple Factors
+* The function of '''''GH3-8''''' is tissue-specific, growth stage–specific, and signal molecule–regulated.
 ==Labs working on this gene==
+* National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural
-Wang Shiping, National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural
+University, Wuhan 430070, China
 ==References==
 <references>
 <ref name="ref1">
-Takase, T., Nakazawa, M., Ishikawa, A., Kawashima, M., Ichikawa, T., Takahashi, N. et al. (2004) ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation. Plant J. 37:471–483.</ref>
+Ding X, Cao Y, Huang L, Zhao J, Xu C, Li X, Wang S. Activation of the
+indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and
-<ref name="ref2">
+promotes salicylate- and jasmonate-independent basal immunity in rice. Plant
- Staswick, P.E., Serban, B., Rowe, M., Tiryaki, I., Maldonado, M.T., Maldonado, M.C. et al. (2005) Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid. Plant Cell 17: 616–627.</ref>
+Cell. 2008 Jan;20(1):228-40. doi: 10.1105/tpc.107.055657. PubMed PMID: 18192436;
 </references>

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Evolution===
-Please input evolution information here.
+GH3 family genes are divided into three groups (I, II, and III) based on their sequence similarities and the substrate specificities of their products in Arabidopsis, which harbors 19 GH3 members and one incomplete GH3 protein. Group I GH3 enzymes are JA-amido or SAamido synthetases. Arabidopsis Group II enzymes were demonstrated to be active on IAA. Group III enzymes have only been identified in Arabidopsis to date. Members of this gene family are known to be regulated by phytohormones and biotic/abiotic stress factors, including abscisic acid (ABA), SA, JA, drought, cold, salt, pathogen infection, and light. GH3-mediated auxin homeostasis is an essential constituent of the complex network of auxin activity that regulates stress adaptation responses.GH3 family genes were divided into three groups (I, II, and III) based on their sequence similarities and the substrate specificities of their products in Arabidopsis, which harbors 19 GH3 members and one incomplete GH3 protein. Group III GH3 enzymes, of which there are nine in Arabidopsis, have only been identified in Arabidopsis to date.
 [[File:Figure 2 dyf.jpg]]
 '''Figure 2 Phylogenetic analysis and gene structure of apple GH3 members.'''
 ==Labs working on this gene==

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 Wang Shiping, National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural
 University, Wuhan 430070, China.
 Usha Vijayraghavan, Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India.

← Older revision		Revision as of 12:47, 5 June 2014
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	[[File:Figure 2 dyf.jpg]]		[[File:Figure 2 dyf.jpg]]
		+
		+	'''Figure 2 Phylogenetic analysis and gene structure of apple GH3 members.''' A hylogeny of apple GH3 proteins, generated using MEGA5 (using the neighbor-joining method and a bootstrap test with 1000 iterations). B Gene structure of the corresponding apple GH3 proteins, generated by a gene structure display server. The black boxes represent exons and lines represent introns. 0, 1, and 2 represent phase 0, 1, and 2 introns.

	==Labs working on this gene==		==Labs working on this gene==