Os07g0666900 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T08:49:20Z

‎Structured Information

2015-04-18T13:19:02Z

‎Function

2015-04-15T07:58:03Z

2015-04-13T06:19:43Z

‎Expression

2015-01-09T16:13:12Z

‎Labs working on this gene

2015-01-09T16:10:56Z

‎References

2015-01-09T16:10:07Z

‎Knowledge Extension

2015-01-09T16:09:08Z

‎Evolution

2015-01-09T16:07:41Z

‎Location

2015-01-09T16:06:38Z

‎Expression

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Function===
 *OsNHX1, functions as a Na<sup>+</sup>and H<sup>+</sup>exchanger and plays important roles in salt tolerance of rice<ref name="ref1"/><ref name="ref2"/><ref name="ref3"/><ref name="ref4"/><ref name="ref5"/><ref name="ref6"/>. ''OsNHX1'' may play an important role in the salt tolerance of shoots rather than roots<ref name="ref5"/>.
-−
 *''OsNHX1'' plays important roles in the compartment of Na<sup>+</sup> from the cytoplasm of cells into the vacuoles in both of the tissues<ref name="ref2"/><ref name="ref5"/>.

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 *''OsNHX1'' has the ability to suppress Na+, Li+ and hygromycin sensitivity of yeast ''nhx1'' mutants and sensitivity to a high K+ concentration, a novel phenotype of the ''nhx1'' mutants<ref name="ref5"/>.
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 *''OsNHX1'' gene encodes a vacuolar (Na<sup>+</sup>, K<sup>+</sup>)/H<sup>+</sup> antiporter that has activity similar to that of the ''ScNHX1'' protein<ref name="ref5"/>.
 *Expression of Na<sup>+</sup>/H<sup>+</sup> exchanger gene OsNHX was also enhanced by submergence stress and the level of enhancement appeared to agree with the level of seedling vigor among the four cultivars studied, indicating the necessity of further study of its role in seedling vigor under submergence in rice<ref name="ref7"/>.

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 ===Expression===
-In young leaves, three OsNHX1-OE(T4, T5, T6) lines showed high expression by the genes of interests<ref name="ref1"/>.
+*In young leaves, three OsNHX1-OE(T4, T5, T6) lines showed high expression by the genes of interests<ref name="ref1"/>.
 *The expression of ''OsNHX1'' was differently expressed in roots and shoots, and was stimulated by salt stress in both of the tissues, suggesting that ''OsNHX1'' plays important roles in the compartment of Na<sup>+</sup>from the cytoplasm of cells into the vacuoles in both of the tissues<ref name="ref2"/>.

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 ==Labs working on this gene==
-Please input related labs here.
+*State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
 ==References==

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 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==

← Older revision		Revision as of 16:10, 9 January 2015
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	===Knowledge Extension===		===Knowledge Extension===
		+	The observed mode of ''OsACS'', ''OsACO'' and ''OsNHX'' expression under submergence suggests that these genes can be potential targets for understanding the mechanism regulating seedling vigor under submergence at the post-germination stage in rice<ref name="ref7"/>.

		+	The Na<sup>+</sup>/H<sup>+</sup>exchanger that catalyzes the exchange of Na<sup>+</sup>for H<sup>+</sup>across membranes, contributes to regulation of internal pH, cell volume, and sodium level in the cytoplasm<ref name="ref10"/>.
		+
		+
		+	Na<sup>+</sup>/H<sup>+</sup> antiporters, which catalyze the exchange of Na<sup>+</sup> for H<sup>+</sup> across membranes, contribute to the regulation of internal pH, cell volume and the sodium level in the cytoplasm<ref name="ref11"/><ref name="ref12"/>. The antiporters are widespread membrane proteins found in animals, yeasts, bacteria and plants. In particular, vacuolar Na<sup>+</sup>/H<sup>+</sup> antiporters, which compartmentalize Na<sup>+</sup> into the vacuoles for detoxification, have been investigated as the key to salt tolerance in yeasts and plants<ref name="ref13"/>.

	==Labs working on this gene==		==Labs working on this gene==

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 ===Evolution===
-Please input evolution information here.
+[[File: Na,H exchanger proteins.jpg|right|thumb|250px|'''Figure 2.''''' Phylogenetic analysis of Na<sup>+</sup>/H<sup>+</sup> exchanger proteins.(from reference <ref name="ref2"/>).'']]
-You can also add sub-section(s) at will.
+*Phylogenetic analysis of various Na<sup>+</sup>/H<sup>+</sup> exchangers indicated that ''OsNHX1'', ''AtNHX1'', ''NHX1'', and ''NHE6'' share a cluster distinguished from previously known clusters of other exchangers corresponding to the plasma membrane isoforms of NHEs, bacterial Nha, and yeast Sod2-like exchangers(Fig. 2). ''OsNHX1'' has low similarity with Sod2 of ''S. pombe'', and NhaA and NhaB of ''E. coli''. Phylogenetic analysis revealed that these exchangers share distinct groups from NHX1 and NHEs (Fig. 2)<ref name="ref2"/>.
 ===Knowledge Extension===

← Older revision		Revision as of 16:07, 9 January 2015
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	===Location===		===Location===
		+	*''Nass and Rao''<ref name="ref8"/> showed that NHX1 was localized in prevacuolar compartments and a member of the newly identified cluster that shared intracellular localization. In addition, NHE6 has been reported to have a mitochondrial distribution<ref name="ref9"/> ,which suggesting that ''OsNHX1'' is localized in the intracellular membrane.

		+	*subcellular localization:<br>
		+	Analysis using rice cells expressing a fusion protein of ''OsNHX1'' and green fluorescent protein and Western blot analysis using antibodies specific for ''OsNHX1'' confirmed the localization of ''OsNHX1'' on the tonoplasts, which indicating that the ''OsNHX1'' gene encodes a vacuolar (Na<sup>+</sup>, K<sup>+</sup>)/H<sup>+</sup> antiporter<ref name="ref5"/>.

	===Evolution===		===Evolution===