Os07g0682800 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T08:49:57Z

‎Structured Information

Huaidong Wu: /* Labs working on this gene */

2014-06-06T07:44:37Z

‎Labs working on this gene

Huaidong Wu: /* References */

2014-06-06T07:44:09Z

‎References

Huaidong Wu: /* References */

2014-06-06T07:41:23Z

‎References

Huaidong Wu: /* Labs working on this gene */

2014-06-06T07:38:48Z

‎Labs working on this gene

Huaidong Wu: /* Evolution */

2014-06-06T07:36:20Z

‎Evolution

Huaidong Wu: /* Evolution */

2014-06-06T07:26:31Z

‎Evolution

Huaidong Wu: /* Annotated Information */

2014-06-06T07:24:05Z

‎Annotated Information

Huaidong Wu: /* Function */

2014-06-06T06:52:40Z

‎Function

Huaidong Wu: /* Annotated Information */

2014-06-06T06:47:33Z

‎Annotated Information

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Takahisa Yuo*1, Masanori Toyota1, Masahiko Ichii2 and Shin Taketa3)
 )Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa 761-0795, Japan
 ) Kagawa University, 1-1 Saiwai, Takamatsu, Kagawa 760-8521, Japan
 ) Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan

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 ==References==
 .Cormack,R.G.H. (1935) Investigations  on the development of root hairs. New Phytologist 34: 30–54.
 .OsRAF is an ethylene responsive and root abundant factor gene of rice Yibing Hu; Kang Chong; Tai Wang Plant Growth Regulation, 2008, 54(1): 55-61
 .Favery,B., E.Ryan, J.Foreman, P.Linstead, K.Boudonck, M.Steer, P.Shaw and L. Dolan (2001)  KOJAK encodes a cellulose synthase-like protein required for root hair cell morphogenesis in Arabidopsis. Genes Dev. 15: 79–89.
 ==Structured Information==

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 ==References==
-Please input cited references here.
+.Cormack,R.G.H. (1935) Investigations  on the development of root hairs. New Phytologist 34: 30–54.
 ==Structured Information==

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 ==Labs working on this gene==
-Please input related labs here.
+Takahisa Yuo*1, Masanori Toyota1, Masahiko Ichii2 and Shin Taketa3)
 ==References==

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-F1 progeny derived from a cross between  rth1 and Kasalath showed normal root hairs and plant growth. F2 progeny segregated 1561 plants with normal phenotype and 527 root-hairless plants with short root phenotype, fitting to a 3 : 1 ratio (χ 2 =0.80, 0.3<p<0.4). Therefore, rth1 is considered a recessive mutation. In a population of 116 F2 plants, the rth1 gene mapped roughly to the distal region of the long arm of chromosome 7. For fine mapping, a total of 2,088 F2 plants were studied. The  rth1 gene was flanked by STS markers, RK30 and RK20, with the distances of 0.26cM and 0.05 cM, respectively (Fig. 3A). The physical distance between them is about 38 kb. In the rth1 candidate region,
+     F1 progeny derived from a cross between  rth1 and Kasalath showed normal root hairs and plant growth. F2 progeny segregated 1561 plants with normal phenotype and 527 root-hairless plants with short root phenotype, fitting to a 3 : 1 ratio (χ 2 =0.80, 0.3<p<0.4). Therefore, rth1 is considered a recessive mutation. In a population of 116 F2 plants, the rth1 gene mapped roughly to the distal region of the long arm of chromosome 7. For fine mapping, a total of 2,088 F2 plants were studied. The  rth1 gene was flanked by STS markers, RK30 and RK20, with the distances of 0.26cM and 0.05 cM, respectively (Fig. 3A). The physical distance between them is about 38 kb. In the rth1 candidate region,
 three genes are predicted in public databases. Those includenucleic acid binding protein (AK109592), expressed protein
 (AK065098) and putative apyrase protein (AK066262). The entire coding region of all these genes was sequenced.Comparison of the DNA sequences between WT and rth1 detected a single nucleotide change only in the putative apyrase(OsAPY; ATPdiphosphohydrolase  EC.3.6.1.5) gene, and
 the sequences of two other genes are identical between WT and rth1. Compared to the OsAPY gene of WT, rth1 carries a nucleotide substitution (G→A) in the junction between the third intron and the forth exon (Fig. 3C, D). This substitution caused the splicing anomaly to the rth1 cDNA sequence that shifted the splicing site 5-bp downstream and produced a premature stop codon, resulting in 185-amino acid peptide
 instead of normal 467-amino acid protein. We confirmed alternation of splicing in rth1 by cDNA sequencing.
 ==Labs working on this gene==

← Older revision		Revision as of 07:26, 6 June 2014
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−	~~You can also add sub~~-~~section~~(s) ~~at will~~.	+	F1 progeny derived from a cross between rth1 and Kasalath showed normal root hairs and plant growth. F2 progeny segregated 1561 plants with normal phenotype and 527 root-hairless plants with short root phenotype, fitting to a 3 : 1 ratio (χ 2 =0.80, 0.3<p<0.4). Therefore, rth1 is considered a recessive mutation. In a population of 116 F2 plants, the rth1 gene mapped roughly to the distal region of the long arm of chromosome 7. For fine mapping, a total of 2,088 F2 plants were studied. The rth1 gene was flanked by STS markers, RK30 and RK20, with the distances of 0.26cM and 0.05 cM, respectively (Fig. 3A). The physical distance between them is about 38 kb. In the rth1 candidate region,
		+	three genes are predicted in public databases. Those includenucleic acid binding protein (AK109592), expressed protein
		+	(AK065098) and putative apyrase protein (AK066262). The entire coding region of all these genes was sequenced.Comparison of the DNA sequences between WT and rth1 detected a single nucleotide change only in the putative apyrase(OsAPY; ATPdiphosphohydrolase EC.3.6.1.5) gene, and
		+	the sequences of two other genes are identical between WT and rth1. Compared to the OsAPY gene of WT, rth1 carries a nucleotide substitution (G→A) in the junction between the third intron and the forth exon (Fig. 3C, D). This substitution caused the splicing anomaly to the rth1 cDNA sequence that shifted the splicing site 5-bp downstream and produced a premature stop codon, resulting in 185-amino acid peptide
		+	instead of normal 467-amino acid protein. We confirmed alternation of splicing in rth1 by cDNA sequencing.

	==Labs working on this gene==		==Labs working on this gene==

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 ===Expression===
-Please input expression information here.
+In Arabidopsis, a lot of root hair mutants are available, and many genes involved in the root hair morphogenesis have been isolated (Grierson and Schiefelbein 2002). On the other hand, rice has a more complicated pattern of root hair formation (Kawata and Ishihara 1959). Only two root hair mutants in rice have been reported (Suzuki et al. 2003, Kim et al. 2007), and, causal gene was isolated in only one mutant (Kim  et al. 2007). A mutant gene, root hairless 1 (rth1) reported by Suzuki et al. (2003), remains unidentified. Thus, genetic studies on root hairs in rice lag behind compared to Arabidopsis. In the present study, we have identified a new root hair mutant gene, rth1 in rice by means of positional cloning. We describe morphological and physiological characteristics, and gene expression pattern in rth1 mutant. We revealed that
 ===Evolution===
-Please input evolution information here.
 You can also add sub-section(s) at will.

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 Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake and anchorage to the soil.
 ===Function===
+Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake
 ===Expression===

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 ==Annotated Information==
 ===Function===
-Please input function information here.
 ===Expression===