Os08g0114200 - Revision history

Xysj1980 at 03:05, 27 March 2017

2017-03-27T03:05:47Z

Xysj2012: /* Structured Information */

2015-06-12T09:23:58Z

‎Structured Information

Libaolian: /* Annotated Information */

2014-05-27T13:39:41Z

‎Annotated Information

Libaolian: /* Labs working on this gene */

2014-05-27T13:30:41Z

‎Labs working on this gene

Libaolian: /* References */

2014-05-27T13:28:56Z

‎References

Libaolian: /* Annotated Information */

2014-05-27T13:25:42Z

‎Annotated Information

Libaolian: /* Evolution */

2014-05-26T13:50:22Z

‎Evolution

Libaolian at 13:37, 26 May 2014

2014-05-26T13:37:04Z

Libaolian at 08:20, 26 May 2014

2014-05-26T08:20:50Z

Libaolian: /* Annotated Information */

2014-05-26T08:20:10Z

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---李宝连+201328006912019 16:20, 26 May 2014 (CST)The rice '''''Os08g0114200''''' is OsGLU3 which is similar to CEL5=CELLULASE 5 coding a β-1,4-endoglucanase，playing an significant role in root elongation in rice
+'''''Os08g0114200''''' is OsGLU3 which is similar to CEL5=CELLULASE 5 coding a β-1,4-endoglucanase，playing an significant role in root elongation in rice
 ==Annotated Information==
 ===Introduce===

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ==Annotated Information==
 ===Introduce===
-'''Endo-1,4-β-D-glucanases (EGases)''' form a large family of hydrolytic enzymes in prokaryotes and eukaryotes.In higher plants, potential substratesin vivo are xyloglucan and non-crystalline cellulose in the cell wall.Gene expression patterns suggest a role for EGases in various developmental processes such as leaf abscission,fruit ripening and cell expansion. Using Arabidopsis thaliana genetics, scientists demonstrate the requirement of a specialized member of the EGase family for the correct assembly of the walls of elongating cells.'''KORRIGAN(KOR)''' is identified by an extreme dwarf mutant with pronounced architectural alterations in the primary cell wall. The '''KORgene''' was isolated and encodes a membrane-anchored member of the EGase family, which is highly conserved between mono- and  icotyledonous plants. '''KOR''' is located primarily in the plasma membrane and presumably acts at the plasma membrane–cell wall interface.'''KORmRNA''' was found in all organs examined, and in the developing darkgrown hypocotyl, mRNA levels were correlated with rapid cell elongation. Among plant growth factors involved in the control of hypocotyl elongation (auxin, gibberellins and ethylene) none significantly influenced KOR-mRNA levels. However, reducedKOR-mRNA levels were observed in det2, a mutant deficient for brassinosteroids. Although the in vivo substrate remains to be determined, the mutant phenotype is consistent with a central role for KOR in the assembly of the cellulose–hemicellulose network in the expanding cell wall.
+'''Endo-1,4-β-D-glucanases (EGases)''' form a large family of hydrolytic enzymes in prokaryotes and eukaryotes.In higher plants, potential substratesin vivo are xyloglucan and non-crystalline cellulose in the cell wall.Gene expression patterns suggest a role for EGases in various developmental processes such as leaf abscission,fruit ripening and cell expansion. Using Arabidopsis thaliana genetics, scientists demonstrate the requirement of a specialized member of the EGase family for the correct assembly of the walls of elongating cells.'''KORRIGAN(KOR)''' is identified by an extreme dwarf mutant with pronounced architectural alterations in the primary cell wall. The '''KORgene''' was isolated and encodes a membrane-anchored member of the EGase family, which is highly conserved between mono- and  icotyledonous plants. '''KOR''' is located primarily in the plasma membrane and presumably acts at the plasma membrane–cell wall interface.'''KORmRNA''' was found in all organs examined, and in the developing darkgrown hypocotyl, mRNA levels were correlated with rapid cell elongation. Among plant growth factors involved in the control of hypocotyl elongation (auxin, gibberellins and ethylene) none significantly influenced KOR-mRNA levels. However, reducedKOR-mRNA levels were observed in det2, a mutant deficient for brassinosteroids. Although the in vivo substrate remains to be determined, the mutant phenotype is consistent with a central role for KOR in the assembly of the cellulose–hemicellulose network in the expanding cell wall (4).
 ===Function===
 The gene OsGLU3 (Os08g0114200), a β-1,4-endoglucanase, can affect the cellulose synthesis for root elongation in rice. And the phosphate starvation induced root
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 OsGLU1, OsGLU2 and OsGLU3 contained a predicted highly hydrophobic transmembrane motif in the N-terminal and belonged to the type II integral membrane protein anchored in the membrane. The results demonstrated thatOsGLU1, OsGLU2,OsGLU3 and OsGLU10 showed constitutive expression patterns in all the organs tested, and
 the OsGLU4, OsGLU5, OsGLU6, OsGLU9were abundant in roots and developing flowers of plants. The other two genes OsGLU7 and OsGLU8 showed relatively higher expression in rachis and developing flowers. These different expression patterns indicated multiple functions of these genes in different processes of plant growth and development. Specific and combinational expression of these genes may be essential for the formation or function of a given organ（2）.
 ==Labs working on this gene==

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 .State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, People’s Republic of China.
 .Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou , People’s Republic of China.
 ==References==

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 .	Zhou HL, He SJ, Cao YR, Chen T, Du BX, Chu CC, et al. OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation. Plant molecular biology. 2006;60(1):137-51.
 .	Zhang JW, Xu L, Wu YR, Chen XA, Liu Y, Zhu SH, et al. OsGLU3, a putative membrane-bound endo-1,4-beta-glucanase, is required for root cell elongation and division in rice (Oryza sativa L.). Mol Plant. 2012;5(1):176-86.
 ==Structured Information==

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 Please input evolution information here.
-In rice genome, the putative membrane-anchored endo-b-1,4-D-glucanases were encoded by three genes: OsGLU1, OsGLU2, and OsGLU3.
+In rice genome, the putative membrane-anchored endo-b-1,4-D-glucanases were encoded by three genes: OsGLU1, OsGLU2, and OsGLU3.Recently,Libertiniet al.(2004) reported that 15 endoglucanase genes were present in rice genome.that these proteins could be classified into four main clusters. One cluster contained OsGLU4, OsGLU8, OsGLU12, OsGLU13,OsGLU14 and OsGLU15. Another cluster contained OsGLU1, OsGLU2, OsGLU3, KOR and CEL3. The third cluster contained OsGLU5,OsGLU6, OsGLU7, OsGLU9, OsGLU10 and OsGLU11.OsGLU1to OsGLU10 each gene had different numbers of introns and exons. All proteins of the OsGLU family contained the EGase domain. The
 ==Labs working on this gene==

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 ===Expression===
-In the rice genome, endo-1,4-b-D-glucanases form a multiple gene family including OsGLU3 which share high sequence similarity with KOR1 (2). OsGLU3is ubiquitously expressed in various tissues with strong expression in root tip, lateral root, and crown root primodia. OsGLU3contains four exons and three introns (Fig 2B). The putative OsGLU3 was predicted to contain a transmembrane domain, a cytosolic domain, and a catalytic domain ('''Supplemental Fig 3A'''). The mutation is located in the catalytic domain, which is highly conserved among the plant KOR1 homologs ('''Supplemental Fig 3B'''). qRT–PCR showed that the OsGLU3 is highly expressed in root tissue and has relatively lower expression in the other tissues. The OsGLU3–GUS expression was observed ubiquitously in the rice plants included in leaf veins, excoemums,and roots. the OsGLU3–GFP protein may reflect the native OsGLU3.OsGLU3 localizes in the  plasma member and endosomes, and the export of OsGLU3 to the PM depends on vesicle transport. Phosphate starvation could induce root elongation inOsglu3-1.The phosphate starvation-induced primary root elongation and cellulose-content increase are abolished inOsglu3-2, which suggests that phosphate starvation-induced primary root elongation depends on the activity of OsGLU3 (3).
+In the rice genome, endo-1,4-b-D-glucanases form a multiple gene family including OsGLU3 which share high sequence similarity with KOR1 (2). OsGLU3is ubiquitously expressed in various tissues with strong expression in root tip, lateral root, and crown root primodia. OsGLU3contains four exons and three introns (Fig 2B). The putative OsGLU3 was predicted to contain a transmembrane domain, a cytosolic domain, and a catalytic domain ('''Supplemental Fig 3A'''). The mutation is located in the catalytic domain, which is highly conserved among the plant KOR1 homologs ('''Supplemental Fig 3B'''). qRT–PCR showed that the OsGLU3 is highly expressed in root tissue and has relatively lower expression in the other tissues. The OsGLU3–GUS expression was observed ubiquitously in the rice plants included in leaf veins, excoemums,and roots. the OsGLU3–GFP protein may reflect the native OsGLU3.OsGLU3 localizes in the  plasma member and endosomes, and the export of OsGLU3 to the PM depends on vesicle transport. Phosphate starvation could induce root elongation inOsglu3-1.The phosphate starvation-induced primary root elongation and cellulose-content increase are abolished in Osglu3-2, which suggests that phosphate starvation-induced primary root elongation depends on the activity of OsGLU3.Suggesting that a single recessive gene was responsible for the mutant phenotype. Using 1 000 F2 mutant seedlings selected from the population, the mutation was mapped to a 56-kb region between S4-24837K and S4-24893K on chromosome 4. This region contains 14 open reading frames (ORFs), including a b-1,4-endoglucanase (OsGLU3, LOC_Os04g41970)(3).
 ===Evolution===