Os08g0323700 - Revision history

Xysj2012: /* Evolution */

2018-01-16T02:37:27Z

‎Evolution

Xysj2012 at 02:37, 16 January 2018

2018-01-16T02:37:06Z

Rice2012: /* Structured Information */

2015-06-12T09:29:22Z

‎Structured Information

Zhangye: /* Annotated Information */

2014-12-23T06:46:54Z

‎Annotated Information

Zhangye: /* Knowledge Extension */

2014-12-23T06:28:49Z

‎Knowledge Extension

Zhangye: /* Function */

2014-12-23T06:27:19Z

‎Function

Zhangye at 06:26, 23 December 2014

2014-12-23T06:26:55Z

Zhangye: /* Evolution */

2014-12-23T06:19:26Z

‎Evolution

Zhangye: /* Knowledge Extension */

2014-12-23T06:17:41Z

‎Knowledge Extension

Zhangye: /* Evolution */

2014-12-23T06:05:56Z

‎Evolution

@@ Line 27: / Line 27: @@
 ===Evolution===
-*Orthologue CCC genes:
+*Orthologue CCC genes: A complete open reading frame (ORF) was found in the At CCC cDNA sequence, and its comparison with the genomic sequence allowed us to determine the intron/exon structure of the At CCC gene. TBLASTN searches identified orthologue CCC genes in other plant genomes. Two CCC genes were identified in rice (Oryza sativa, japonica), ''OsCCC1'' and ''OsCCC2'', and one more in Medicago truncatula, ''MtCCC''.Interestingly, these plant CCC genes contained 12 introns located in the same position, indicating that the exon/intron structure was perfectly conserved.<ref name="ref2" />
 Plant and animal CCCs were highly conserved in the C-termini and the central hydrophobic core, particularly within the putative Tm domains and the predicted intracellular loops.<ref name="ref2" />

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-''Xiang-Qiang Kong et al.'' have cloned an Oryza sativa cDNA encoding for a member of the cation–Cl- cotransporter (CCC) family. Plant CCC proteins are highly conserved and that they have greater sequence similarity to the sub-family of animal K+ –Cl- cotransporters than to other cation–Cl- cotransporters. In plants CCCs are involved in the homeostasis of Cl-.<ref name="ref1" /><ref name="ref2" /><br>
+* ''Xiang-Qiang Kong et al.'' have cloned an Oryza sativa cDNA encoding for a member of the cation–Cl- cotransporter (CCC) family. Plant CCC proteins are highly conserved and that they have greater sequence similarity to the sub-family of animal K+ –Cl- cotransporters than to other cation–Cl- cotransporters. In plants CCCs are involved in the homeostasis of Cl-.<ref name="ref1" /><ref name="ref2" /><br>
-''OsCCC1'' was primarily involved in K+ homeostasis and Cl- homeostasis, it might be an K+ -Cl- cotransporter. It is a compact phylogenetic cluster with highly conserved genes that shows the highest similarity to animal KCCs. ''OsCCC1'' plays a significant role in ion homeostasis and rice development under saline conditions. The analysis of ''OsCCC1'' RNAi lines designed by ''Xiang-Qiang Kong et al.'' strongly suggests its involvement in pivotal developmental processes.<ref name="ref1" />
+* ''OsCCC1'' was primarily involved in K+ homeostasis and Cl- homeostasis, it might be an K+ -Cl- cotransporter. It is a compact phylogenetic cluster with highly conserved genes that shows the highest similarity to animal KCCs. ''OsCCC1'' plays a significant role in ion homeostasis and rice development under saline conditions. The analysis of ''OsCCC1'' RNAi lines designed by ''Xiang-Qiang Kong et al.'' strongly suggests its involvement in pivotal developmental processes.<ref name="ref1" />
 ===Mutation===
-WT plants VS. Transgenic plants<ref name="ref1" />:<br>
+* WT plants VS. Transgenic plants<ref name="ref1" />:<br>
 [[File: OsCCC1 wt.png|right|thumb|400px|'''Figure 1.'''''WT plants VS. Transgenic plants (from reference <ref name="ref1" />).'']]
 *Southern blot analysis of genomic DNA from the RNAi plants confirmed that ''OsCCC1'' was integrated into the genome of the transgenic plants and that more than half of the transgenic lines contained two or more copies of the gene. (Figure 1A)
 *Real-time PCR analysis to examine the expression levels of ''OsCCC1''in the T3 plants of the transgenic lines revealed that the expression level of ''OsCCC1'' in RNAi transgenic lines Ri 3-4 and Ri 7-2 had decreased to 30 and 39%, respectively. (Figure 1B)
-*The effect of NaCl and KCl on the germination of ''OsCCC1'' RNAi seeds was also tested.There was no difference in seed germination between the WT and RNAi
+*The effect of NaCl and KCl on the germination of ''OsCCC1'' RNAi seeds was also tested.There was no difference in seed germination between the WT and RNAi seedlings under normal conditions under normal conditions. In the presence of 150 mM NaCl, the germination of both WT and ''OsCCC1'' RNAi seeds was inhibited slightly, but there was no obvious difference between the WT and RNAi seedlings. In the presence of KCl, the germination of ''OsCCC1''RNAi seeds was inhibited significantly and WT seeds inhibited to a lesser extent. When the seeds were subjected to the 50 mM NaCl + 100 mM KCl treatment, approximately 96% of WT seeds germinated compared with only 83% of the seeds of the RNAi plants. Under 150 mM KCl treatment, 82% of the WT seeds germinated compared with only 41% seeds of the RNAi plants.(Figure 1C) Thus, during germination, gene silencing of ''OsCCC1'' in rice decreased the tolerance to KCl but not to NaCl.
 *The progeny of T3 homozygous kanamycin-tolerant plants (T4 generation) of lines Ri 3-4 and Ri 7-2 and WT seedlings were cultivated under salt stress (NaCl and KCl)or non-salt-stress conditions. In contrast to WT plants, the transgenic plants of line Ri 7-2 displayed progressive chlorosis, reduced leaf size, and a general growth inhibition under KCl conditions. These inhibitory effects increased progressively with increasing KCl concentrations. (Figure 1D)
 *Similar phenomena occurred in the progeny of line Ri 3-4. These seedlings were harvested and their fresh and dry weight measured. Under normal conditions, the fresh and dry weight of ''OsCCC1'' RNAi lines was lower than those of WT seedlings. The KCl and NaCl treatments significantly decreased the fresh weight and dry weight of both WT and RNAi seedlings; however, compared to normal conditions, the fresh and dry weight of RNAi seedlings decreased more obviously than those of the WT under NaCl and KCl conditions, especially under KCl conditions.Of the three stress treatments, the RNAi lines showed the greatest decrease in shoot and root fresh and dry weight compared to the WT under the 150 mM KCl treatment. (Figure 1E–H)

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 6: / Line 6: @@
 ''OsCCC1'' was primarily involved in K+ homeostasis and Cl- homeostasis, it might be an K+ -Cl- cotransporter. It is a compact phylogenetic cluster with highly conserved genes that shows the highest similarity to animal KCCs. ''OsCCC1'' plays a significant role in ion homeostasis and rice development under saline conditions. The analysis of ''OsCCC1'' RNAi lines designed by ''Xiang-Qiang Kong et al.'' strongly suggests its involvement in pivotal developmental processes.<ref name="ref1" />
-===WT plants VS. Transgenic plants<ref name="ref1" />===
+===Mutation===
 [[File: OsCCC1 wt.png|right|thumb|400px|'''Figure 1.'''''WT plants VS. Transgenic plants (from reference <ref name="ref1" />).'']]
 *Southern blot analysis of genomic DNA from the RNAi plants confirmed that ''OsCCC1'' was integrated into the genome of the transgenic plants and that more than half of the transgenic lines contained two or more copies of the gene. (Figure 1A)
@@ Line 15: / Line 16: @@
 *Similar phenomena occurred in the progeny of line Ri 3-4. These seedlings were harvested and their fresh and dry weight measured. Under normal conditions, the fresh and dry weight of ''OsCCC1'' RNAi lines was lower than those of WT seedlings. The KCl and NaCl treatments significantly decreased the fresh weight and dry weight of both WT and RNAi seedlings; however, compared to normal conditions, the fresh and dry weight of RNAi seedlings decreased more obviously than those of the WT under NaCl and KCl conditions, especially under KCl conditions.Of the three stress treatments, the RNAi lines showed the greatest decrease in shoot and root fresh and dry weight compared to the WT under the 150 mM KCl treatment. (Figure 1E–H)
-===Expression<ref name="ref1"/>===
+===Expression===
 [[File:OsCCC1 ExpressionPattern.png|right|thumb|300px|'''Figure 2.''' ''Expression analyses of ''OsCCC1''(from reference <ref name="ref1"/>).'']]
 *The ''OsCCC1'' transcript levels in the leaves and roots were slightly upregulated by NaCl treatment, but distinctly upregulated by KCl treatment,suggesting that ''OsCCC1''was mainly involved in KCl rather than NaCl transport (Figure 2e, f).

@@ Line 31: / Line 31: @@
 ===Knowledge Extension===
-*Regarding the ions involved in the symport mechanism, CCCs are divided into three groups: K+ : Cl－cotransporters, known as the KCC group; Na+ : Cl－) cotransporters, NCC group; and Na+ :K+ :Cl－ cotransporters, NKCC group. Ion transport studies have demonstrated that the members of all three groups shared an absolute requirement for both  Cl－ and at least one cation (Na+ and/or K+), and that the three cotransport processes are electrically silent or electroneutral. <ref name="ref2" /> <ref name="ref3" />
+*Regarding the ions involved in the symport mechanism, CCCs are divided into three groups: K+ : Cl－cotransporters, known as the KCC group; Na+ : Cl－cotransporters, NCC group; and Na+ :K+ :Cl－ cotransporters, NKCC group. Ion transport studies have demonstrated that the members of all three groups shared an absolute requirement for both  Cl－ and at least one cation (Na+ and/or K+), and that the three cotransport processes are electrically silent or electroneutral. <ref name="ref2" /> <ref name="ref3" />
 *When grown with unmodified soil, ''ccc'' mutants were observed to produce seed that over-accumulate Ca and S, and underaccumulate Na and K. CCC encodes the only known cation chloride co-transporter in ''A. thaliana.'' Promoter-GUS and public expression profiling analyses suggest that CCC is expressed throughout the plant, including roots, shoots, seeds and pollen.<ref name="ref3" />

← Older revision		Revision as of 06:26, 23 December 2014
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−	The rice ''OsCCC1'' is a member of the cation-Cl- cotransporter (CCC) family and plays a significant role in K+ and Cl－ homeostasis and rice plant development.<ref name="ref1" />)	+	The rice ''OsCCC1'' is a member of the cation-Cl- cotransporter (CCC) family and plays a significant role in K+ and Cl－ homeostasis and rice plant development.<ref name="ref1" />

	==Annotated Information==		==Annotated Information==