Os08g0499300 - Revision history

Rice2012: /* Structured Information */

2015-06-12T09:32:18Z

‎Structured Information

Zhong6937991: /* Evolution */

2014-05-31T05:01:21Z

‎Evolution

Zhong6937991: /* Expression */

2014-05-31T04:59:06Z

‎Expression

Zhong6937991: /* Mutation */

2014-05-31T04:56:43Z

‎Mutation

Zhong6937991: /* Function */

2014-05-31T04:55:35Z

‎Function

Zhong6937991: /* Function */

2014-05-31T04:51:39Z

‎Function

Zhong6937991: Undo revision 174794 by Zhong6937991 (talk)

2014-05-31T02:16:36Z

Undo revision 174794 by Zhong6937991 (talk)

Zhong6937991: /* Function */

2014-05-31T02:13:25Z

‎Function

Zhong6937991: /* References */

2014-05-29T13:59:29Z

‎References

Zhong6937991: /* References */

2014-05-29T13:58:58Z

‎References

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Evolution===
-It is hypothesized that WRKY genes originated from two eukaryotic species, Giardia lamblia and Dictyostelium discoideum, and have since then been greatly amplified in plant species<ref name="ref2" />.The WRKY30 containes an entire ORF in length of 2022 bp and encodes a protein of 674 amino acid residues consisting of two WRKY domains each with a zinc finger motif of C2H2 and a nuclear localization signal, belonging to the WRKY subgroup I. WRKY30 shares high amino acid sequence identity with those from monocots such as wheat (Hordeum vulgare), Sorghum bicolor and Brachypodium sylvaticum. The identity are 61.2%,57.0% and 56.0%, respectively(fig8)<ref name="ref6" />.[[File:Fig.10.jpg|200px|thumb|right|Fig. 8 Phylogenetic tree of WRKY30 with WRKY proteins from other species. At, Arabidosis thaliana (AF418308_1); Bs, Brachypodium sylvaticum (CAJ26376); Hv, Hordeum vulgare (ABI13407); Os, Oryza sativa (ADK13079); Pt, Populus trichocarpa (XP_002330532); Rc, Ricinus communis (XP_002529203); Sb, Sorghum bicolor (XP_002444812); Sl, Solanum lycopersicum (CAC36402); Ta, Triticum aestivum (CAJ19358); Vv, Vitis vinifera (XP_002265612); Zm, Zea mays (NP_001169214)]]
+It is hypothesized that ''WRKY'' genes originated from two eukaryotic species, ''Giardia lamblia'' and ''Dictyostelium discoideum'', and have since then been greatly amplified in plant species<ref name="ref2" />.The ''WRKY30'' containes an entire ORF in length of 2022 bp and encodes a protein of 674 amino acid residues consisting of two WRKY domains each with a zinc finger motif of C2H2 and a nuclear localization signal, belonging to the WRKY subgroup I. WRKY30 shares high amino acid sequence identity with those from monocots such as wheat (''Hordeum vulgare''), ''Sorghum bicolor'' and ''Brachypodium sylvaticum''. The identity are 61.2%,57.0% and 56.0%, respectively(fig8)<ref name="ref6" />.[[File:Fig.10.jpg|200px|thumb|right|Fig. 8 Phylogenetic tree of WRKY30 with WRKY proteins from other species. At, ''Arabidosis thaliana'' (AF418308_1); Bs, ''Brachypodium sylvaticum'' (CAJ26376); Hv, ''Hordeum vulgare'' (ABI13407); Os, ''Oryza sativa'' (ADK13079); Pt, ''Populus trichocarpa'' (XP_002330532); Rc, ''Ricinus communis'' (XP_002529203); Sb, ''Sorghum bicolor'' (XP_002444812); Sl, ''Solanum lycopersicum'' (CAC36402); Ta, ''Triticum aestivum'' (CAJ19358); Vv, ''Vitis vinifera'' (XP_002265612); Zm, ''Zea mays'' (NP_001169214)]]
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 ===Expression===
-[[File:Fig.4.png|200px|thumb|right|Fig.4 The expression of OsWRKY30 induced by blast infection in the presence of Pi36.]][[File:Fig.5.png|200px|thumb|right|Fig.5 Induced expression of the WRKY30 gene. Plants are treated with salicylic acid (SA) and methyl jasmonate (MeJA).]][[File:Fig.6.png|200px|thumb|right|Fig.6 Expression analysis of OsWRKY30 under stress and hormone treatments.  A. Expression level of OsWRKY30 under 100 uM ABA treatment (time course: 0,1, 3, 6, 12, and 24 h).  B. Expression level of OsWRKY30 under drought stress (time course: 0, 1, 3, 6,12, and 24 h).]]
+[[File:Fig.4.png|200px|thumb|right|Fig.4 The expression of OsWRKY30 induced by blast infection in the presence of Pi36.]][[File:Fig.5.png|200px|thumb|right|Fig.5 Induced expression of the ''WRKY30'' gene. Plants are treated with salicylic acid (SA) and methyl jasmonate (MeJA).]][[File:Fig.6.png|200px|thumb|right|Fig.6 Expression analysis of OsWRKY30 under stress and hormone treatments.  A. Expression level of OsWRKY30 under 100 uM ABA treatment (time course: 0,1, 3, 6, 12, and 24 h).  B. Expression level of OsWRKY30 under drought stress (time course: 0, 1, 3, 6,12, and 24 h).]]
-WRKY30 exists as a single copy in the rice genome. The transcript levels of WRKY30 are relatively high in stem, leaf and grain, next are root and panicle, and the least was flower(fig.7)<ref name="ref6" />.[[File:Fig.9.jpg|200px|thumb|right|Fig. 7 Expression patterns of WRKY30 in different rice organs]]
+''WRKY30'' exists as a single copy in the rice genome. The transcript levels of ''WRKY30'' are relatively high in stem, leaf and grain, next are root and panicle, and the least was flower(fig.7)<ref name="ref6" />.[[File:Fig.9.jpg|200px|thumb|right|Fig. 7 Expression patterns of WRKY30 in different rice organs]]
-It is first induced at 1 h p.i. in cv.Kasalath. Its expression level increased up to maximum by 24 h p.i., decreased slightly by 48 h p.i., produced a second peak around 72 h p.i., and finally declined by 96 h p.i.(fig.4)<ref name="ref7" />.
+It is first induced at 1 h p.i. in ''cv.Kasalath''. Its expression level increased up to maximum by 24 h p.i., decreased slightly by 48 h p.i., produced a second peak around 72 h p.i., and finally declined by 96 h p.i.(fig.4)<ref name="ref7" />.
-As shown in Fig. 5, the WRKY30 transcripts began to accumulate at 1 h and strongly increased during 6–12 h after MeJA treatment, followed by a sharp decrease to the background level at 24 h. Also, the expression of WRKY30 increased rapidly but modestly within 1 h, continued to increase at 6 h, and maintained at a steady level till 24 h after SA treatment<ref name="ref4" />.
+As shown in Fig. 5, the ''WRKY30'' transcripts began to accumulate at 1 h and strongly increased during 6–12 h after MeJA treatment, followed by a sharp decrease to the background level at 24 h. Also, the expression of WRKY30 increased rapidly but modestly within 1 h, continued to increase at 6 h, and maintained at a steady level till 24 h after SA treatment<ref name="ref4" />.
-The expression of OsWRKY30 is induced after treatments with ABA and drought stress (Fig. 6). The expression of OsWRKY30 gene is investigated using Real-time PCR. OsWRKY30 is significantly increased by treatments with 100 uM ABA, and the expression reached the highest expression (by 13.7 times) at 12 h after treatments (Fig. 6A). A similar OsWRKY30 expression pattern is found after drought stress treatments (Fig. 6B)<ref name="ref5" />.
+The expression of OsWRKY30 is induced after treatments with ABA and drought stress (Fig. 6). The expression of ''OsWRKY30'' gene is investigated using Real-time PCR. OsWRKY30 is significantly increased by treatments with 100 uM ABA, and the expression reached the highest expression (by 13.7 times) at 12 h after treatments (Fig. 6A). A similar OsWRKY30 expression pattern is found after drought stress treatments (Fig. 6B)<ref name="ref5" />.
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 ===Mutation===
-Overexpression of WRKY30 in rice shows enhanced disease resistance to the bacterial pathogen Xoo(fig.1), sheath blight fungus Rhizoctonia solani(fig.2) as well as M. oryzae<ref name="ref3" /><ref name="ref4" />.
+Overexpression of ''WRKY30'' in rice shows enhanced disease resistance to the bacterial pathogen ''Xoo''(fig.1), sheath blight fungus ''Rhizoctonia solani''(fig.2) as well as ''M. oryzae''<ref name="ref3" /><ref name="ref4" />.
-Overexpression of OsWRKY30 in rice dramatically increased drought tolerance(fig.3). Mutations in the phosphorylation target sites of OsWRKY30 have been shown to cause the loss of function of drought tolerance. That result suggests that OsWRKY30 phosphorylation is important for its biological functions in defense responses to abiotic stress<ref name="ref5" />.
+Overexpression of ''OsWRKY30'' in rice dramatically increased drought tolerance(fig.3). Mutations in the phosphorylation target sites of OsWRKY30 have been shown to cause the loss of function of drought tolerance. That result suggests that OsWRKY30 phosphorylation is important for its biological functions in defense responses to abiotic stress<ref name="ref5" />.
 ===Expression===

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 ==Annotated Information==
 ===Function===
-[[File:Fig.1.png|200px|thumb|right|Fig.1 A representative photograph taken at twelve days after inoculation. OsWRKY30OX plants show enhanced resistance to Xoo.]][[File:Fig.2.png|200px|thumb|right|Fig.2 Disease symptoms in wild-type (WT) and WRKY30 overexpression (lines 2-3 and 17-2) plants at 7 days after inoculation (DPI) with Rhizoctonia solani.]][[File:Fig.3.png|200px|thumb|right|Fig.3 Overexpression of OsWRKY30 in rice exhibited more tolerance to water deficit stress than did wild type plants.]]
+[[File:Fig.1.png|200px|thumb|right|Fig.1 A representative photograph taken at twelve days after inoculation. ''OsWRKY30OX'' plants show enhanced resistance to ''Xoo''.]][[File:Fig.2.png|200px|thumb|right|Fig.2 Disease symptoms in wild-type (WT) and ''WRKY30'' overexpression (lines 2-3 and 17-2) plants at 7 days after inoculation (DPI) with ''Rhizoctonia solani''.]][[File:Fig.3.png|200px|thumb|right|Fig.3 Overexpression of ''OsWRKY30'' in rice exhibited more tolerance to water deficit stress than did wild type plants.]]
 ''OsWRKY30'' encodes a protein named WRKY30 transcription factor, which is a member of Group Ia of rice WRKY family with two DNA-binding domains, designated the WRKY domain, containing a highly conserved WRKYGQK residue as well as a novel zinc finger motif. Both the conserved elements of the domain are necessary for high binding affinity of WRKY30 to a canonical W-box containing a sequence of TTGACC/T <ref name="ref1" /><ref name="ref2" />.
-WRKY30 is rapidly induced by pathogen M. grisea infection, bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and related phytohormones SA and JA <ref name="ref2" />.OsWRKY30 functions as a positive regulator of rice disease resistance via an SA signaling pathway. It can up-regulate the SA-responsive gene OsWRKY45<ref name="ref3" />.WRKY30 is also associated with activated expression of JA synthesis-related genes LOX, AOS2 and pathogenesis-related (PR)3 and PR10, and increased endogenous JA accumulation under the challenge of fungal pathogens<ref name="ref4" />.
+WRKY30 is rapidly induced by pathogen ''M. grisea'' infection, bacterial pathogen ''Xanthomonas oryzae pv. oryzae (Xoo)'' and related phytohormones SA and JA <ref name="ref2" />.OsWRKY30 functions as a positive regulator of rice disease resistance via an SA signaling pathway. It can up-regulate the SA-responsive gene ''OsWRKY45''<ref name="ref3" />. WRKY30 is also associated with activated expression of JA synthesis-related genes ''LOX, AOS2'' and pathogenesis-related ''(PR)3'' and ''PR10'', and increased endogenous JA accumulation under the challenge of fungal pathogens<ref name="ref4" />.
 OsWRKY30 is also involved drought tolerance. OsWRKY30 interacts with the rice mitogen-activated protein kinases(MAPKs), OsMPK3, OsMPK4, OsMPK7, OsMPK14, OsMPK20-4 and OsMPK20-5, and can be phosphorylated by OsMPK3, OsMPK7, and OsMPK14<ref name="ref5" />.

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 .	<ref name="ref8">Lee, H., Ko, Y. J., Cha, J. Y., Park, S. R., Ahn, I., & Hwang, D. J. (2013). The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes. Plant Biotechnology Reports, 7(3), 221-230.</ref>
 </references>
 ==Structured Information==

← Older revision		Revision as of 13:58, 29 May 2014
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	8. <ref name="ref8">Lee, H., Ko, Y. J., Cha, J. Y., Park, S. R., Ahn, I., & Hwang, D. J. (2013). The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes. Plant Biotechnology Reports, 7(3), 221-230.</ref>		8. <ref name="ref8">Lee, H., Ko, Y. J., Cha, J. Y., Park, S. R., Ahn, I., & Hwang, D. J. (2013). The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes. Plant Biotechnology Reports, 7(3), 221-230.</ref>
		+	</references>
		+