Os08g0509600 - Revision history

Rice2012: /* Structured Information */

2015-06-12T09:32:56Z

‎Structured Information

Wangmin2013: /* Evolution */

2014-06-06T15:50:41Z

‎Evolution

Wangmin2013: /* Localization */

2014-06-06T15:47:18Z

‎Localization

Wangmin2013: /* Evolution */

2014-06-06T15:42:36Z

‎Evolution

Wangmin2013: /* Localization */

2014-06-06T15:42:10Z

‎Localization

Wangmin2013: /* Localization */

2014-06-06T15:41:13Z

‎Localization

Wangmin2013: /* Localization */

2014-06-06T15:40:52Z

‎Localization

Wangmin2013: /* Localization */

2014-06-06T15:40:11Z

‎Localization

Wangmin2013: /* Evolution */

2014-06-06T15:38:00Z

‎Evolution

Wangmin2013: /* Evolution */

2014-06-06T15:30:12Z

‎Evolution

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 29: / Line 29: @@
 ===Evolution===
 [[File:Phylogenetic tree and homology analysis of SPL proteins.jpg|right|thumb|320px|'''Figure 5.''' ''Phylogenetic tree and homology analysis of SPL proteins(from reference) <ref name="ref2" />.'']]
-*Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and Arabidopsis thaliana. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.
+*Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and ''Arabidopsis thaliana''. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.
 *SPL protein are plant-specific transcription factors containing a highly conserved DNA-binding domain called an SBP-box. The founder members of the SPL family, SBP1 and SBP2, were identified in Antirrhinum majus as proteins that bind to a sequence motif present in the promoter of SQUAMOSA (SQUA), the MADS-box gene which specifies flower meristem identity. SPL genes are widely conserved in both dicots and monocots. In maize, a few SPL genes have been identified, including liguleless1 (LG1), teosinte glume architecture 1 (tga1) and tasselsheath4 (TSH4)<ref name="ref4" />.

@@ Line 36: / Line 36: @@
 ===Localization===
 [[File:subcellular localization of the OsSPL14-GFP fusion protein.jpg|right|thumb|320px|'''Figure 7.''' '' subcellular localization of the OsSPL14-GFP fusion protein(from reference)<ref name="ref3" />.'']]
 *Subcellular localization analysis using green fluorescent protein (GFP) fusion OsSPL14 proteins detected the GFP signal localized to nuclei (Figure 6a). Additionally, analysis using the yeast one-hybrid system showed that the OsSPL14 protein had transcription-activating abilities (Figure 6b). Figure 6 shows subcellular localization and transactivation analysis of OsSPL14 protein. (a) Subcellular localization of OsSPL14 was analyzed using the GFP fusion OsSPL14 protein driven by the 35S promoter. Nuclei were stained with DAPI. (b) The full-length cDNA of OsSPL14 and SK1-Full, as a positive control, were fused to the GAL4-DNA binding domain. The pGBKT7-vector is the negative control<ref name="ref2" />.

@@ Line 28: / Line 28: @@
 ===Evolution===
-[[File:Phylogenetic tree and homology analysis of SPL proteins.jpg|right|thumb|320px|'''Figure 5..''' ''Phylogenetic tree and homology analysis of SPL proteins(from reference) <ref name="ref2" />.'']]
+[[File:Phylogenetic tree and homology analysis of SPL proteins.jpg|right|thumb|320px|'''Figure 5.''' ''Phylogenetic tree and homology analysis of SPL proteins(from reference) <ref name="ref2" />.'']]
 *Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and Arabidopsis thaliana. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.

@@ Line 36: / Line 36: @@
 ===Localization===
-[[File:subcellular localization of the OsSPL14-GFP fusion protein.jpg|right|thumb|320px|'''Figure 5.''' '' subcellular localization of the OsSPL14-GFP fusion protein(from reference)<ref name="ref3" />.'']]
+[[File:subcellular localization of the OsSPL14-GFP fusion protein.jpg|right|thumb|320px|'''Figure 7.''' '' subcellular localization of the OsSPL14-GFP fusion protein(from reference)<ref name="ref3" />.'']]
 *Subcellular localization analysis using green fluorescent protein (GFP) fusion OsSPL14 proteins detected the GFP signal localized to nuclei (Figure 6a). Additionally, analysis using the yeast one-hybrid system showed that the OsSPL14 protein had transcription-activating abilities (Figure 6b). Figure 6 shows subcellular localization and transactivation analysis of OsSPL14 protein. (a) Subcellular localization of OsSPL14 was analyzed using the GFP fusion OsSPL14 protein driven by the 35S promoter. Nuclei were stained with DAPI. (b) The full-length cDNA of OsSPL14 and SK1-Full, as a positive control, were fused to the GAL4-DNA binding domain. The pGBKT7-vector is the negative control<ref name="ref2" />.
-*OsSPL14 is localized to the nucleus (Figure 7), consistent with a role as a transcription factor<ref name="ref3" />.
+*OsSPL14 is localized to the nucleus (Figure 6 and 7), consistent with a role as a transcription factor<ref name="ref3" />.
 ===Knowledge Extension===

← Older revision		Revision as of 15:38, 6 June 2014
Line 28:		Line 28:

	===Evolution===		===Evolution===
		+	[[File:Phylogenetic tree and homology analysis of SPL proteins.jpg\|right\|thumb\|320px\|'''Figure 5..''' ''Phylogenetic tree and homology analysis of SPL proteins(from reference) <ref name="ref2" />.'']]
	*Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and Arabidopsis thaliana. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.		*Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and Arabidopsis thaliana. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.

← Older revision		Revision as of 15:30, 6 June 2014
Line 28:		Line 28:

	===Evolution===		===Evolution===
		+	*Phylogenetic analysis indicated that the OsSPL gene at Os08g0509600 is categorized as OsSPL14 and is conserved in sorghum, wheat, maize and Arabidopsis thaliana. Figure 5 shows the phylogenetic tree and homology analysis of SPL proteins. (a) Phylogenetic tree of SPL proteins among rice, sorghum, wheat, maize, and Arabidopsis. OsSPL14/WFP is highlighted by the red rectangle. (b) Homology analysis of SPL domains of the WFP clade. The SBP-domain includes the Zn-1, Zn-2, and NLS domains<ref name="ref2" />.
		+
	*SPL protein are plant-specific transcription factors containing a highly conserved DNA-binding domain called an SBP-box. The founder members of the SPL family, SBP1 and SBP2, were identified in Antirrhinum majus as proteins that bind to a sequence motif present in the promoter of SQUAMOSA (SQUA), the MADS-box gene which specifies flower meristem identity. SPL genes are widely conserved in both dicots and monocots. In maize, a few SPL genes have been identified, including liguleless1 (LG1), teosinte glume architecture 1 (tga1) and tasselsheath4 (TSH4)<ref name="ref4" />.		*SPL protein are plant-specific transcription factors containing a highly conserved DNA-binding domain called an SBP-box. The founder members of the SPL family, SBP1 and SBP2, were identified in Antirrhinum majus as proteins that bind to a sequence motif present in the promoter of SQUAMOSA (SQUA), the MADS-box gene which specifies flower meristem identity. SPL genes are widely conserved in both dicots and monocots. In maize, a few SPL genes have been identified, including liguleless1 (LG1), teosinte glume architecture 1 (tga1) and tasselsheath4 (TSH4)<ref name="ref4" />.