Os08g0566600 - Revision history

Chenxi Sang: /* Phenotypic analysis */

2016-10-28T12:49:52Z

‎Phenotypic analysis

Chenxi Sang at 12:49, 28 October 2016

2016-10-28T12:49:38Z

Zhennan: /* Evolution */

2016-06-25T10:02:03Z

‎Evolution

Rice2012: /* Structured Information */

2015-06-12T09:35:33Z

‎Structured Information

Longkailian: /* Function */

2014-05-28T08:40:15Z

‎Function

Longkailian: /* Function */

2014-05-28T08:35:51Z

‎Function

Longkailian: /* Function */

2014-05-28T07:04:11Z

‎Function

Longkailian: /* Function */

2014-05-28T07:02:09Z

‎Function

Longkailian at 03:26, 28 May 2014

2014-05-28T03:26:25Z

Longkailian: /* Function */

2014-05-24T09:52:48Z

‎Function

@@ Line 12: / Line 12: @@
 * As occurred in the Arabidopsis pgr5 mutant, non-photochemical quenching of Chl fluorescence (NPQ) induction was impaired in the leaves, but the electron transport rate (ETR) was only mildly affected at high light intensity.
 * The P700+ level was reduced even at low light intensity, suggesting that the PGR5 function was severely disturbed as in the Arabidopsis pgr5 mutant and that the other alternative routes of electrons could not compensate the stromal redox balance.
-* The amplitude of the light–dark electrochromic shift (ECS) signal (ECSt), which reflects the total size of the proton
+* The amplitude of the light–dark electrochromic shift (ECS) signal (ECSt), which reflects the total size of the proton motive force in steady-state photosynthesis, was reduced by 13–25% at approximately the growth light intensity.
 * The CO2 fixation rate was only slightly reduced in the '''''PGR5''''' KD lines. Despite the drastic reduction in NPQ and P700+ levels, total biomass was only slightly reduced in '''''PGR5''''' KD lines grown at 370 mmol photons m–2s–1.

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os08g0566600''''' was reported as '''''PGR5''''' in 2012 <ref name="ref1" /> by researchers from Japan.
 ==Annotated Information==
 ===Function===
-PGR5(PROTON GRADIENT REGULATION 5)gene is required for Rice photosystem I(PSI) cyclic electron transpor.The fucntions of PGR5 as follow:
+* The '''''PGR5''''' (PROTON GRADIENT REGULATION 5) in Arabidopsis is required for PSI cyclic electron transport.
+* The rice '''''PGR5''''' is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.
-.PGR5-dependent PSI cyclic electron transporthelps to restore the balance of the oxidation of chloroplasts.
+===Phenotypic analysis===
+* In three '''''PGR5''''' knockdown (KD) lines, the PGR5 protein level was reduced to 5–8% of that in the wild type, resulting in a 50% reduction in PGRL1 (PGR5-LIKE PHOTOSYNTHETIC PHENOTYPE 1) protein levels.
-.PGR5 plays an key role in maintaining reduction capability the iron oxide-dependent protein bodies quinone when the rice chloroplast damage.
+* In ruptured chloroplasts, ferredoxin-dependent plastoquinone reduction activity was partially impaired; the phenotype was mimicked by addition of antimycin A to wild-type chloroplasts.
+* As occurred in the Arabidopsis pgr5 mutant, non-photochemical quenching of Chl fluorescence (NPQ) induction was impaired in the leaves, but the electron transport rate (ETR) was only mildly affected at high light intensity.
-.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.
+* The P700+ level was reduced even at low light intensity, suggesting that the PGR5 function was severely disturbed as in the Arabidopsis pgr5 mutant and that the other alternative routes of electrons could not compensate the stromal redox balance.
-[[File:1.jpg.jpg|right|thumb|150px|'''Figure 1'''''(A) Schematic model of Fd-dependent PQ reduction in ruptured chloroplasts. ]]
+* The amplitude of the light–dark electrochromic shift (ECS) signal (ECSt), which reflects the total size of the proton
+motive force in steady-state photosynthesis, was reduced by 13–25% at approximately the growth light intensity.
-[[File:2.jpg.jpg|right|thumb|150px|'''Figure 2''''' (B) Fd-dependent PQ reduction activity in ruptured chloroplasts isolated from various lines. ]]
+* The CO2 fixation rate was only slightly reduced in the '''''PGR5''''' KD lines. Despite the drastic reduction in NPQ and P700+ levels, total biomass was only slightly reduced in '''''PGR5''''' KD lines grown at 370 mmol photons m–2s–1.
 ==Labs working on this gene==
-.Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
+* Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
-.CREST, Japan Science and Technology Agency, Chiyoda-ku, Tokyo, 102-0076 Japan
+==References==
+<references>
-.Department of Applied Plant Science, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan
+* <ref name="ref1">
-.National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan
+</references>
 ==Structured Information==

← Older revision		Revision as of 10:02, 25 June 2016
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	===Evolution===		===Evolution===
−	~~Please input evolution information here.~~
−
	You can also add sub-section(s) at will.		You can also add sub-section(s) at will.

@@ Line 39: / Line 39: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 08:40, 28 May 2014
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	[[File:1.jpg.jpg\|right\|thumb\|150px\|'''Figure 1'''''(A) Schematic model of Fd-dependent PQ reduction in ruptured chloroplasts. ]]		[[File:1.jpg.jpg\|right\|thumb\|150px\|'''Figure 1'''''(A) Schematic model of Fd-dependent PQ reduction in ruptured chloroplasts. ]]

		+	[[File:2.jpg.jpg\|right\|thumb\|150px\|'''Figure 2''''' (B) Fd-dependent PQ reduction activity in ruptured chloroplasts isolated from various lines. ]]
	4.CO(2) fixation and growth rate are very robust in the face of alterations in the fundamental reactions of photosynthesis under constant light conditions in rice.		4.CO(2) fixation and growth rate are very robust in the face of alterations in the fundamental reactions of photosynthesis under constant light conditions in rice.

← Older revision		Revision as of 08:35, 28 May 2014
Line 13:		Line 13:

	3.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.		3.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.
		+	[[File:1.jpg.jpg\|right\|thumb\|150px\|'''Figure 1'''''(A) Schematic model of Fd-dependent PQ reduction in ruptured chloroplasts. ]]

	4.CO(2) fixation and growth rate are very robust in the face of alterations in the fundamental reactions of photosynthesis under constant light conditions in rice.		4.CO(2) fixation and growth rate are very robust in the face of alterations in the fundamental reactions of photosynthesis under constant light conditions in rice.

← Older revision		Revision as of 07:04, 28 May 2014
Line 13:		Line 13:

	3.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.		3.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.
		+
		+	4.CO(2) fixation and growth rate are very robust in the face of alterations in the fundamental reactions of photosynthesis under constant light conditions in rice.

	===Expression===		===Expression===

← Older revision		Revision as of 07:02, 28 May 2014
Line 11:		Line 11:

	2.PGR5 plays an key role in maintaining reduction capability the iron oxide-dependent protein bodies quinone when the rice chloroplast damage.		2.PGR5 plays an key role in maintaining reduction capability the iron oxide-dependent protein bodies quinone when the rice chloroplast damage.
		+
		+	3.PGR5 is required for ferredoxin (Fd)-dependent plastoquinone (PQ) reduction in ruptured chloroplasts of rice.

	===Expression===		===Expression===

← Older revision		Revision as of 03:26, 28 May 2014
Line 1:		Line 1:
	Please input one-sentence summary here.		Please input one-sentence summary here.

−	The rice Os08g0566600 was report as proton gradient regulation 5 ,which is is ~~screened from the Rice Data~~.	+	The rice Os08g0566600 was report as proton gradient regulation 5 ,which is involved in electron flow in rice Photosystem I. It is essential for photoprotection.