Os09g0306400 - Revision history

Zhennan: /* Knowledge Extension */

2016-07-01T08:59:54Z

‎Knowledge Extension

Zhennan: /* Expression */

2016-07-01T08:56:56Z

‎Expression

Zhennan: /* Expression */

2016-07-01T08:56:36Z

‎Expression

Zhennan: /* Mutation */

2016-07-01T08:55:59Z

‎Mutation

Zhennan: /* Mutation */

2016-07-01T08:55:25Z

‎Mutation

Zhennan: /* Subcellular localization */

2016-07-01T08:54:51Z

‎Subcellular localization

Zhennan: /* Evolution */

2016-07-01T08:54:36Z

‎Evolution

Zhennan: /* Mutation */

2016-07-01T08:54:05Z

‎Mutation

Zhennan: /* Knowledge Extension */

2016-07-01T08:53:39Z

‎Knowledge Extension

Zhennan: /* Knowledge Extension */

2016-07-01T08:53:20Z

‎Knowledge Extension

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 ===Knowledge Extension===
 [[File:ABA signaling pathways.jpg|right|thumb|300px|'''Figure 2.'''''Summary of ABA signaling pathways that result in drought stress resistant responses.(from reference <ref name="ref4"/>).'']]
-*Unlike other cereal species, rice is able to germinate and elongate under anoxia. The regulatory mechanism that configures the transcriptome of rice during anaerobic germination is yet to be established. Although some rice varieties exhibit some degree of tolerance to anaerobic stress that normally occurs during partial (hypoxia) or complete submergence (anoxia), it remains a major factor that limits productivity specially for the rainfed lowland cropping systems in Southeast Asia, where an average family relies on subsistence rice farming<ref name="ref2"/><ref name="ref5"/><ref name="ref6"/>.
+* Unlike other cereal species, rice is able to germinate and elongate under anoxia. The regulatory mechanism that configures the transcriptome of rice during anaerobic germination is yet to be established. Although some rice varieties exhibit some degree of tolerance to anaerobic stress that normally occurs during partial (hypoxia) or complete submergence (anoxia), it remains a major factor that limits productivity specially for the rainfed lowland cropping systems in Southeast Asia, where an average family relies on subsistence rice farming<ref name="ref2"/><ref name="ref5"/><ref name="ref6"/>.
 *The ability of rice to germinate under anoxia by extending the coleoptile is a highly unusual characteristic and a key feature underpinning the ability of rice seeds to establish in such a stressful environment. The process has been a focal point for research for many years. However, the molecular mechanisms underlying the anoxic growth of coleoptile still remain largely unknown.
-*Combined with recent findings on ABA signal transduction in Arabidopsis (Fig. 2), investigation into the genetic regulation of core ABA signal transduction components could promote the introduction of drought tolerance responses into various crop species. Since the identification of PYR/PYL/RCARs, a better understanding of signaling networks among the canonical ABA signaling components has shed light on the genetic modification of ABA signal transduction for improving drought tolerance in plants<ref name="ref4"/>.
+* Combined with recent findings on ABA signal transduction in Arabidopsis (Fig. 2), investigation into the genetic regulation of core ABA signal transduction components could promote the introduction of drought tolerance responses into various crop species. Since the identification of PYR/PYL/RCARs, a better understanding of signaling networks among the canonical ABA signaling components has shed light on the genetic modification of ABA signal transduction for improving drought tolerance in plants<ref name="ref4"/>.
-*''OsbZIP71'' is a member of anoxia stressed up-regulated transcription factors with potential significance to the pattern of cis-element enrichment among the upregulated genes<ref name="ref2"/><ref name="ref3"/>.
+* ''OsbZIP71'' is a member of anoxia stressed up-regulated transcription factors with potential significance to the pattern of cis-element enrichment among the upregulated genes<ref name="ref2"/><ref name="ref3"/>.
-*Major targets for this purpose include PYR/PYL/RCARs-PP2CSnRK2s complexes as well as their phosphorylation substrates such as transcription factors or channel proteins. A PP2C binds to a SnRK2 in the absence of ABA and mediate dephosphorylation of the SnRK2 (Fig. 2). On the other hand, activation of SnRK2s as a critical positive signal for ABA signaling usually begins with autophosphorylation of SnRK2s<ref name="ref4"/>.
+* Major targets for this purpose include PYR/PYL/RCARs-PP2CSnRK2s complexes as well as their phosphorylation substrates such as transcription factors or channel proteins. A PP2C binds to a SnRK2 in the absence of ABA and mediate dephosphorylation of the SnRK2 (Fig. 2). On the other hand, activation of SnRK2s as a critical positive signal for ABA signaling usually begins with autophosphorylation of SnRK2s<ref name="ref4"/>.
 ==Labs working on this gene==

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 **In the salt treatments, ''OsbZIP71'' was repressed both in shoots and roots within 0.5–6 h, and then restored to its basal expression level at 12 h.
 **''OsbZIP71'' was not changed at different time-points under normal condition, which suggesting that ''OsbZIP71'' may function in abiotic stress signal transduction.
-*those transgenic lines tolerance to salt and PEG osmotic stresses were positive correlated to relative expression of ''OsbZIP71'' and
+*those transgenic lines tolerance to salt and PEG osmotic stresses were positive correlated to relative expression of ''OsbZIP71'' and ''OsbZIP71'' may regulate responses to salt and PEG osmotic stresses. ''OsbZIP71'' overexpression significantly increased rice drought tolerance at both seedling and reproductive stages.
 *In a word, Overexpression of ''OsbZIP71'' enhanced drought and salt tolerance in rice. ''OsbZIP71'' RNAi lines was sensitive to drought and salt stress in rice. Inducible expression of ''OsbZIP71'' enhanced drought tolerance but sensitive to salt in rice<ref name="ref1"/>.

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 *those transgenic lines tolerance to salt and PEG osmotic stresses were positive correlated to relative expression of ''OsbZIP71'' and
 ''OsbZIP71'' may regulate responses to salt and PEG osmotic stresses. ''OsbZIP71'' overexpression significantly increased rice drought tolerance at both seedling and reproductive stages.
 *In a word, Overexpression of ''OsbZIP71'' enhanced drought and salt tolerance in rice. ''OsbZIP71'' RNAi lines was sensitive to drought and salt stress in rice. Inducible expression of ''OsbZIP71'' enhanced drought tolerance but sensitive to salt in rice<ref name="ref1"/>.
 ===Subcellular localization===

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 *two inducible expression lines (IE-3 and IE-4)
 *and wild type They were used for germination tests. All transgenic lines were independent homozygous and single copy transgenic lines identified by Southern blot<ref name="ref1"/>: <br>
-   '''OsbZIP71''' IE lines show insensitivity to ABA during seed germination.<br>
+   ''OsbZIP71'' IE lines show insensitivity to ABA during seed germination.<br>
-   '''OsbZIP71''' RNAi seedlings show hypersensitivity to ABA.
+   ''OsbZIP71'' RNAi seedlings show hypersensitivity to ABA.
 ===Expression===

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 *two inducible expression lines (IE-3 and IE-4)
 *and wild type They were used for germination tests. All transgenic lines were independent homozygous and single copy transgenic lines identified by Southern blot<ref name="ref1"/>: <br>
-''OsbZIP71'' IE lines show insensitivity to ABA during seed germination.<br>
+  '''OsbZIP71''' IE lines show insensitivity to ABA during seed germination.<br>
-''OsbZIP71'' RNAi seedlings show hypersensitivity to ABA.
+  '''OsbZIP71''' RNAi seedlings show hypersensitivity to ABA.
 ===Expression===

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 ===Subcellular localization===
-To determine the subcellular localization of ''OsbZIP71'', the ORF of ''OsbZIP71'' was fused to GFP in the pA7-GFP vector ''OsbZIP71'' is a nuclear-localized protein (Fig. 1d–f) and may function as a TF<ref name="ref1"/>.
+* To determine the subcellular localization of ''OsbZIP71'', the ORF of ''OsbZIP71'' was fused to GFP in the pA7-GFP vector ''OsbZIP71'' is a nuclear-localized protein (Fig. 1d–f) and may function as a TF<ref name="ref1"/>.
 ===Evolution===

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 ===Evolution===
-NCBI BLASTp results also showed that ''OsbZIP71'' was similar to Group S members of the Arabidopsis bZIP family, such as AtbZIP53, AtbZIP2/GBF5, AtbZIP44, and AtbZIP11/ATB2. Based on homology of the bZIP domains, the 16 members of rice Group S can be divided into three subgroups, designated S1, S2, and S3 (Fig. 1b). Further classification using MEGA4.0 with the known classified bZIP TFs indicated that ''OsbZIP71'' belongs to Group S1 of the bZIP family<ref name="ref1"/>(Fig. 1b)
+* NCBI BLASTp results also showed that ''OsbZIP71'' was similar to Group S members of the Arabidopsis bZIP family, such as AtbZIP53, AtbZIP2/GBF5, AtbZIP44, and AtbZIP11/ATB2. Based on homology of the bZIP domains, the 16 members of rice Group S can be divided into three subgroups, designated S1, S2, and S3 (Fig. 1b). Further classification using MEGA4.0 with the known classified bZIP TFs indicated that ''OsbZIP71'' belongs to Group S1 of the bZIP family<ref name="ref1"/>(Fig. 1b)
 ===Knowledge Extension===