Os09g0395300 - Revision history

Xysj1981 at 06:31, 8 March 2017

2017-03-08T06:31:29Z

Xysj1981 at 06:31, 8 March 2017

2017-03-08T06:31:08Z

Rice2012: /* Structured Information */

2015-06-12T09:41:04Z

‎Structured Information

Chenyaping at 15:23, 30 May 2014

2014-05-30T15:23:14Z

Chenyaping at 12:03, 30 May 2014

2014-05-30T12:03:11Z

Chenyaping at 12:02, 30 May 2014

2014-05-30T12:02:32Z

Chenyaping at 11:55, 30 May 2014

2014-05-30T11:55:41Z

Chenyaping at 11:51, 30 May 2014

2014-05-30T11:51:45Z

Chenyaping at 11:47, 30 May 2014

2014-05-30T11:47:39Z

Chenyaping: /* Annotated Information */

2014-05-30T11:32:24Z

‎Annotated Information

← Older revision		Revision as of 06:31, 8 March 2017
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	===Expression===		===Expression===
−	~~[[File: ZhangF5.jpg\|right\|thumb\|100px\|Fig 5. ''SLL1'' expression pattern analysis <ref name="ref1" />.]]~~
−
	Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Fig. 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel) <ref name="ref1" />.		Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Fig. 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel) <ref name="ref1" />.

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-[[File: ZhangF1.jpg|right|thumb|100px|Fig 1. ''sll1'' has extremely incurved leaves, with deficiency of sclerenchymatous cells at the abaxial side <ref name="ref1" />.]]  [[File: ZhangF3.jpg|right|thumb|100px|Fig 3. Map-based cloning of ''SLL1'', which encodes an MYB transcription factor <ref name="ref1" />.]]  [[File: ZhangF8.jpg|right|thumb|100px|Fig 8. ''Sll1'' alters adaxial-abaxial pattern formation <ref name="ref1" />.]]  [[File: ZhangF10.jpg|right|thumb|100px|Fig 10. ''SLL1'' Affected multiple developmental processes in rice <ref name="ref1" />.]]
+[[File: ZhangF1.jpg|right|thumb|100px|Fig 1. ''sll1'' has extremely incurved leaves, with deficiency of sclerenchymatous cells at the abaxial side <ref name="ref1" />.]]  [[File: ZhangF3.jpg|right|thumb|100px|Fig 3. Map-based cloning of ''SLL1'', which encodes an MYB transcription factor <ref name="ref1" />.]]  [[File: ZhangF8.jpg|right|thumb|100px|Fig 8. ''Sll1'' alters adaxial-abaxial pattern formation <ref name="ref1" />.]]
 ''SLL1'' arrests normal sclerenchymatous cell formation and controls leaf rolling, controls the establishment of rice leaf polarity, and modulates ohotosynthesis by regulating cell fate <ref name="ref1" />.

@@ Line 36: / Line 36: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 33: / Line 33: @@
 <references>
 <ref name="ref1">Zhang GH, et al. (2009) ''SHALLOT-LIKE1'' is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. The Plant Cell Online 21: 719-735.</ref>
 ==Structured Information==

← Older revision		Revision as of 12:03, 30 May 2014
Line 1:		Line 1:
−	The rice ''SHALLOT-LIKE1'' ('' SLL1'') gene, a member of KANADI family, is a key gene controlling rice leaf rolling.	+	The rice ''SHALLOT-LIKE1'' (''SLL1'') gene, a member of KANADI family, is a key gene controlling rice leaf rolling.

	==Annotated Information==		==Annotated Information==

@@ Line 5: / Line 5: @@
 [[File: ZhangF1.jpg|right|thumb|100px|Fig 1. ''sll1'' has extremely incurved leaves, with deficiency of sclerenchymatous cells at the abaxial side <ref name="ref1" />.]]  [[File: ZhangF3.jpg|right|thumb|100px|Fig 3. Map-based cloning of ''SLL1'', which encodes an MYB transcription factor <ref name="ref1" />.]]  [[File: ZhangF8.jpg|right|thumb|100px|Fig 8. ''Sll1'' alters adaxial-abaxial pattern formation <ref name="ref1" />.]]  [[File: ZhangF10.jpg|right|thumb|100px|Fig 10. ''SLL1'' Affected multiple developmental processes in rice <ref name="ref1" />.]]
-To investigate the molecular mechanisms of rice leaf rolling, a rice mutant population (''Oryza sativa'' L. ssp. ''japonica'' variety Nipponbare) generated by ethyl methanesulphonate mutagenesis was screened. Two allelic mutants with extremely incurved leaves were identified, designated as ''sll1-1'' and ''sll1-2'' (Fig. 1A ). ''sll1'', a rice mutant with shallot-like Leaves, displays abnormal sclerenchymatous cell development in the abaxial cell layers, altered mesophyll cell distribution, increased amounts of chlorophyll, and enhanced photosynthesis <ref name="ref1" />.
+''SLL1'' arrests normal sclerenchymatous cell formation and controls leaf rolling, controls the establishment of rice leaf polarity, and modulates ohotosynthesis by regulating cell fate <ref name="ref1" />.
 Genetic analysis revealed that the abnormal character of ''sll1'' was controlled by a single recessive gene. To isolate the relevant mutant gene, ''SLL1'' was mapped to the long arm of rice chromosome 9 between markers RM1896 and RM3700. A large F2 mapping population was then generated, allowing the fine-mapping of ''SLL1'' to a 29.57-kb region, using the sequence tagged site and simple sequence repeat markers (Fig. 3A). Three annotated candidate genes, encoding a hypothetical protein, an En/Spm-like transposon, and a transcription factor containing a MYB-like domain, respectively, were located in this region. Further amplification of the relevant DNA fragments and sequence comparison revealed differences in ''sll1-1'' and ''sll1-2'' alleles in the gene encoding the transcription factor containing the MYB-like domain. Comparison with the corresponding genomic sequence revealed that the ''SLL1'' gene consists of six exons and five introns. The gene encodes a 377–amino acid MYB family transcription factor (Fig. 3B). Homologous analysis showed that ''SLL1'' shares high similarity with the KANADI family members in ''Arabidopsis''.
@@ Line 16: / Line 18: @@
 [[File: ZhangF5.jpg|right|thumb|100px|Fig 5. ''SLL1'' expression pattern analysis <ref name="ref1" />.]]
-Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Fig. 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel).
+Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Fig. 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel) <ref name="ref1" />.
 To assess the expression pattern comprehensively, β-glucuronidase (GUS) activity was examined histochemically in transgenic plants carrying an ''SLL1'' promoter-GUS reporter gene. Results showed that ''SLL1'' was transcribed in stem (Fig. 5B, panels 1 and 2), anthers of young or mature flowers (Fig. 5B, panels 3 to 5), pistil tip (Fig. 5B, panel 6), glume (Fig. 5B, panel 7), vascular tissues of mature seeds (Fig. 5B, panels 8 and 9), coleoptile and embryonic root of germinating seedlings (Fig. 5B, panel 10), and root vascular tissues (Fig. 5B, panel 11). In addition, ''SLL1'' is highly transcribed in leaf veins and leaf sheath (Fig. 5C, panels 1 to 3), guard cells, and tracheal elements (Fig. 5C, panels 4 and 5).
@@ Line 23: / Line 25: @@
 ===Evolution===
 ==Labs working on this gene==

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-[[File:ZhangF1.jpg|right|thumb|200px|Fig 1. ''sll1'' has extremely incurved leaves, with deficiency of sclerenchymatous cells at the abaxial side <ref name="ref1" />.]]  [[File:ZhangF3.jpg|right|thumb|200px|Fig 3. Map-based cloning of ''SLL1'', which encodes an MYB transcription factor <ref name="ref1" />.]]
+[[File: ZhangF1.jpg|right|thumb|200px|Fig 1. ''sll1'' has extremely incurved leaves, with deficiency of sclerenchymatous cells at the abaxial side <ref name="ref1" />.]]  [[File: ZhangF3.jpg|right|thumb|200px|Fig 3. Map-based cloning of ''SLL1'', which encodes an MYB transcription factor <ref name="ref1" />.]]  [[File: ZhangF8.jpg|right|thumb|200px|Fig 8. ''Sll1'' alters adaxial-abaxial pattern formation <ref name="ref1" />.]]  [[File: ZhangF10.jpg|right|thumb|200px|Fig 10. ''SLL1'' Affected multiple developmental processes in rice <ref name="ref1" />.]]
 To investigate the molecular mechanisms of rice leaf rolling, a rice mutant population (''Oryza sativa'' L. ssp. ''japonica'' variety Nipponbare) generated by ethyl methanesulphonate mutagenesis was screened. Two allelic mutants with extremely incurved leaves were identified, designated as ''sll1-1'' and ''sll1-2'' (Fig. 1A ). ''sll1'', a rice mutant with shallot-like Leaves, displays abnormal sclerenchymatous cell development in the abaxial cell layers, altered mesophyll cell distribution, increased amounts of chlorophyll, and enhanced photosynthesis <ref name="ref1" />.
@@ Line 11: / Line 11: @@
 A distinguishing characteristic of the monocot leaf is that microhairs or ligules are present only at the adaxial surface. In ''sll1-1'', the microhairs can be observed on both epidermal surfaces of some plants (Fig. 8A ), and the ligules exist on the abaxial side of the joint (Fig. 8B). This suggests an adaxialized trend in the abaxial epidermis of ''sll1-1'' and indicates the involvement of ''SLL1'' in the development of polarity throughout the leaf abaxial epidermis. ''SLL1'' overexpression resulted in dwarf plants with twisted and abnormal inner rolled leaves. We also observed enlarged phloem in the midrib as well as large and small veins, revealing the enhanced abaxial features of leaves following ''SLL1'' overexpression.
-''SLL1'' affected developmental processes in multiple developmental stages, in accordance with its expression patterns. Aside from altered leaf morphology, other tissues, including seeds, anthers, and roots, displayed abnormal development in ''sll1'' mutants.
+''SLL1'' affected developmental processes in multiple developmental stages, in accordance with its expression patterns. Aside from altered leaf morphology, other tissues, including seeds, anthers, and roots, displayed abnormal development in ''sll1'' mutants (Fig. 10).
 ===Expression===
-[[File:ZhangF5.jpg|right|thumb|200px|Fig 5. ''SLL1'' Expression Pattern Analysis <ref name="ref1" />.]]
+[[File: ZhangF5.jpg|right|thumb|200px|Fig 5. ''SLL1'' expression pattern analysis <ref name="ref1" />.]]
 Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Fig. 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel).