Os09g0396900 - Revision history

Xysj2027: /* Function */

2017-05-07T11:02:19Z

‎Function

Xysj2027: /* Function */

2017-05-07T11:02:05Z

‎Function

Xysj2027 at 11:01, 7 May 2017

2017-05-07T11:01:50Z

Xysj2017: /* Annotated Information */

2017-02-14T14:51:26Z

‎Annotated Information

Rice2012: /* Structured Information */

2015-06-12T09:41:20Z

‎Structured Information

Littlescrew: /* Expression */

2014-06-06T10:14:26Z

‎Expression

Littlescrew: /* Labs working on this gene */

2014-06-06T05:24:28Z

‎Labs working on this gene

Littlescrew: /* References */

2014-06-06T05:19:38Z

‎References

Littlescrew: /* Expression */

2014-06-06T05:14:35Z

‎Expression

Littlescrew: /* Subcellular localization */

2014-06-06T05:13:38Z

‎Subcellular localization

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 ===Function===
-[[File:OsVIT2.jpg|right|thumb|250px|'''Figure 1.'''Subcellular localization of OsVIT1 and OsVIT2<ref name="ref1" />.]]
+[[File:OsVIT2.jpg|right|thumb|327px|'''Figure 1.'''Subcellular localization of OsVIT1 and OsVIT2<ref name="ref1" />.]]
 * OsVIT1 and OsVIT2 may function primarily in flag leaves to mediate vacuolar sequestration of Fe and Zn, due to highly expression levels of ''OsVIT1'' and ''OsVIT2'' in flag leaves rather than embryos.

@@ Line 6: / Line 6: @@
 ===Function===
-[[File:OsVIT2.jpg|right|thumb|250px|'''Figure 1.'''Subcellular localization of OsVIT1 and OsVIT2<ref name="ref1" />.(a–c) Bright-field images of ''Arabidopsis'' protoplasts expressing enhanced green fluorescent protein (EGFP) (a), OsVIT1: EGFP (b) and OsVIT2: EGFP(c).(d–f) Fluorescence images of Arabidopsis protoplasts expressing EGFP (d), OsVIT1: EGFP (e) and OsVIT2: EGFP(f).(g–i) Merged fluorescence images of EGFP (g), OsVIT1: EGFP (h) and OsVIT2: EGFP (i). Bars = 10μm.]]
+[[File:OsVIT2.jpg|right|thumb|250px|'''Figure 1.'''Subcellular localization of OsVIT1 and OsVIT2<ref name="ref1" />.]]
 * OsVIT1 and OsVIT2 may function primarily in flag leaves to mediate vacuolar sequestration of Fe and Zn, due to highly expression levels of ''OsVIT1'' and ''OsVIT2'' in flag leaves rather than embryos.
 * And they might also mediate allocation of Fe/Zn between flag leaves and the grains<ref name="ref1" />.
 ===Phenotypic analysis===

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-Please input one-sentence summary here.
+The rice '''''Os09g0396900''''' was reported as '''''OsVIT2''''' in 2012 by researchers from China.
 ==Annotated Information==
 ===Function===
 [[File:OsVIT2.jpg|right|thumb|250px|'''Figure 1.'''Subcellular localization of OsVIT1 and OsVIT2<ref name="ref1" />.(a–c) Bright-field images of ''Arabidopsis'' protoplasts expressing enhanced green fluorescent protein (EGFP) (a), OsVIT1: EGFP (b) and OsVIT2: EGFP(c).(d–f) Fluorescence images of Arabidopsis protoplasts expressing EGFP (d), OsVIT1: EGFP (e) and OsVIT2: EGFP(f).(g–i) Merged fluorescence images of EGFP (g), OsVIT1: EGFP (h) and OsVIT2: EGFP (i). Bars = 10μm.]]
 OsVIT1 and OsVIT2 may function primarily in flag leaves to mediate vacuolar sequestration of Fe and Zn, due to highly expression levels of ''OsVIT1'' and ''OsVIT2'' in flag leaves rather than embryos. And they might also mediate allocation of Fe/Zn between flag leaves and the grains<ref name="ref1" />. Knockdown of the vacuolar Fe transporter gene OsVIT1 or OsVIT2 may be useful to further increase the Fe concentration of seeds <ref name="ref2" />.
 ===Expression===
-''OsVIT2'' locates in chromosome 9, and encodes putative protein as the vacuolar iron transporter with 247 amino acids which shares 79.8% amino acid similarity with the VIT in ''Arabidopsis''. There are five conserved transmembrane domains in OsVIT2 by further analyses. This gene expresses in flag leaf sheaths with a significantly high level, but it can also be detected in various tissues/organs including embryos at low levels. Different from ''OsVIT2'', ''OsVIT1'' expresses mainly in flag leaf blades, but also occurs ubiquitously in various tissues/organs including embryos with relatively low levels<ref name="ref1" />. OsVIT2 was quickly induced in rice seedling roots and shoots after the treatment of 4.5 mM FeSO4, but OsVIT1 was not highly induced. Fe starvation caused downregulated ''OsVIT2'' expression in roots and shoots, and no OsVIT1 response until 7 days after treatment in roots. These data suggest that OsVIT1 may represent a housekeeping mechanism for Fe accumulation in vacuoles, while OsVIT2 functions more in response to external Fe levels <ref name="ref1" />.
+* This gene expresses in flag leaf sheaths with a significantly high level, but it can also be detected in various tissues/organs including embryos at low levels.
 ===Subcellular localization===
-OsVIT1 and OsVIT2 were localized to vacuolar membrane<ref name="ref1" /> when they combined with enhanced green fluorescent protein and expressed(Figure 1).
+* OsVIT1 and OsVIT2 were localized to vacuolar membrane<ref name="ref1" /> when they combined with enhanced green fluorescent protein and expressed(Figure 1).
 You can also add sub-section(s) at will.

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 ==Annotated Information==
 ===Function===
 OsVIT1 and OsVIT2 may function primarily in flag leaves to mediate vacuolar sequestration of Fe and Zn, due to highly expression levels of ''OsVIT1'' and ''OsVIT2'' in flag leaves rather than embryos. And they might also mediate allocation of Fe/Zn between flag leaves and the grains<ref name="ref1" />. Knockdown of the vacuolar Fe transporter gene OsVIT1 or OsVIT2 may be useful to further increase the Fe concentration of seeds <ref name="ref2" />.
@@ Line 9: / Line 10: @@
 ===Subcellular localization===
 OsVIT1 and OsVIT2 were localized to vacuolar membrane<ref name="ref1" /> when they combined with enhanced green fluorescent protein and expressed(Figure 1).
 You can also add sub-section(s) at will.

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ==Labs working on this gene==
-Please input related labs here.
+*National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
 ==References==

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 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==