Os09g0422500 - Revision history

Rice2012: /* Structured Information */

2015-06-12T09:42:09Z

‎Structured Information

Haoyajing cathy at 06:31, 10 June 2014

2014-06-10T06:31:54Z

Haoyajing cathy at 06:20, 10 June 2014

2014-06-10T06:20:45Z

Haoyajing cathy at 06:18, 10 June 2014

2014-06-10T06:18:26Z

Haoyajing cathy at 06:17, 10 June 2014

2014-06-10T06:17:11Z

Haoyajing cathy at 06:16, 10 June 2014

2014-06-10T06:16:42Z

Haoyajing cathy at 06:16, 10 June 2014

2014-06-10T06:16:21Z

Haoyajing cathy at 06:14, 10 June 2014

2014-06-10T06:14:36Z

Haoyajing cathy at 06:13, 10 June 2014

2014-06-10T06:13:45Z

Haoyajing cathy at 05:08, 10 June 2014

2014-06-10T05:08:36Z

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 .The Rice Annotation Project Database (RAP-DB): hub for Oryza sativa ssp. japonica genome information. Ohyanagi H, et al. Nucleic Acids Res, 2006 Jan 1. PMID 16381971, Free PMC Article
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Introduction===
 A genomic fragment containing the mutant Bc6 gene (9374 bp), including 4.8 kbp of upstream sequence and 492 bp downstream, was digested with HindIII and subcloned into the binary vector pBIGRZ to yield the mutant construct, gBc6/pBIGRZ. The wild-type BC6 gene construct, gBC6/pBIGRZ, was generated by changing the mutated nucleotide at position 7112 from G to wild-type A by PCR mutagenesis with primers, PM-F1 (5′-GCAGTTCCCGCAGAGGTTCGACGGC-3′) and PM-R1 (5′-ATCGACGTCCACGACCGATACGCC-3′). These constructs were introduced into the wild-type plant, T65, by an Agrobacterium (Rhizobium radiobacter)-mediated method using strain EHA101. The T2 generation of transgenic plant was used for cell wall analysis.
-[[File: haoyajing.jpg]]
+[[File: haoyajing.jpg|right|thumb|250px|'''Figure 1.''' ''rice from Brittle phenotype lines VS. Normal lines ]]
 .Promoter

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 ==Annotated Information==
 ===Introduction===
-A genomic fragment containing the mutant Bc6 gene (9374 bp), including 4.8 kbp of upstream sequence and 492 bp downstream, was digested with HindIII and subcloned into the binary vector pBIGRZ to yield the mutant construct, gBc6/pBIGRZ. The wild-type BC6 gene construct, gBC6/pBIGRZ, was generated by changing the mutated nucleotide at position 7112 from G to wild-type A by PCR mutagenesis with primers, PM-F1 (5′-GCAGTTCCCGCAGAGGTTCGACGGC-3′) and PM-R1 (5′-ATCGACGTCCACGACCGATACGCC-3′). These constructs were introduced into the wild-type plant, T65, by an Agrobacterium (Rhizobium radiobacter)-mediated method using strain EHA101. The T2 generation of transgenic plant was used for cell wall analysis.[[File: haoyajing.jpg]]
+A genomic fragment containing the mutant Bc6 gene (9374 bp), including 4.8 kbp of upstream sequence and 492 bp downstream, was digested with HindIII and subcloned into the binary vector pBIGRZ to yield the mutant construct, gBc6/pBIGRZ. The wild-type BC6 gene construct, gBC6/pBIGRZ, was generated by changing the mutated nucleotide at position 7112 from G to wild-type A by PCR mutagenesis with primers, PM-F1 (5′-GCAGTTCCCGCAGAGGTTCGACGGC-3′) and PM-R1 (5′-ATCGACGTCCACGACCGATACGCC-3′). These constructs were introduced into the wild-type plant, T65, by an Agrobacterium (Rhizobium radiobacter)-mediated method using strain EHA101. The T2 generation of transgenic plant was used for cell wall analysis.
 .Promoter

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-The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File: haoyajing.jpg]]
+The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.
 ==Annotated Information==
 ===Introduction===
-A genomic fragment containing the mutant Bc6 gene (9374 bp), including 4.8 kbp of upstream sequence and 492 bp downstream, was digested with HindIII and subcloned into the binary vector pBIGRZ to yield the mutant construct, gBc6/pBIGRZ. The wild-type BC6 gene construct, gBC6/pBIGRZ, was generated by changing the mutated nucleotide at position 7112 from G to wild-type A by PCR mutagenesis with primers, PM-F1 (5′-GCAGTTCCCGCAGAGGTTCGACGGC-3′) and PM-R1 (5′-ATCGACGTCCACGACCGATACGCC-3′). These constructs were introduced into the wild-type plant, T65, by an Agrobacterium (Rhizobium radiobacter)-mediated method using strain EHA101. The T2 generation of transgenic plant was used for cell wall analysis.
+A genomic fragment containing the mutant Bc6 gene (9374 bp), including 4.8 kbp of upstream sequence and 492 bp downstream, was digested with HindIII and subcloned into the binary vector pBIGRZ to yield the mutant construct, gBc6/pBIGRZ. The wild-type BC6 gene construct, gBC6/pBIGRZ, was generated by changing the mutated nucleotide at position 7112 from G to wild-type A by PCR mutagenesis with primers, PM-F1 (5′-GCAGTTCCCGCAGAGGTTCGACGGC-3′) and PM-R1 (5′-ATCGACGTCCACGACCGATACGCC-3′). These constructs were introduced into the wild-type plant, T65, by an Agrobacterium (Rhizobium radiobacter)-mediated method using strain EHA101. The T2 generation of transgenic plant was used for cell wall analysis.[[File: haoyajing.jpg]]
 .Promoter

← Older revision		Revision as of 06:17, 10 June 2014
Line 1:		Line 1:
−	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File:haoyajing.jpg]]	+	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File: haoyajing.jpg]]

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 06:16, 10 June 2014
Line 1:		Line 1:
−	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File:~~桌面/~~haoyajing.jpg]]	+	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File:haoyajing.jpg]]

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 06:14, 10 June 2014
Line 1:		Line 1:
−	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File:图1.jpg]]	+	The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.[[File:桌面/图1.jpg]]

	==Annotated Information==		==Annotated Information==

@@ Line 1: / Line 1: @@
 The Os09g0422500 gene can be called by another gene symbols like BC6 and OsCESA9 which similar to cellulose synthase .The OsCESA9 gene is predominantly expressed in the culms of mature stage plants, consistent with the brittle phenotype in the culm and plays an important role in cell wall biosynthesis and plant growth.
 ==Annotated Information==
 ===Function===
 ;This study characterizes a brittle culm (bc88) mutant of rice (Oryza sativa L.) obtained by ethylene methylsulfonate (EMS)-induced mutagenesis of Wuyunjing 7. The bc88 mutant exhibits a diversity of pleiotropic phenotypes, including brittle culm at the whole-plant growth stages, withered leaf tips at the seedling stage, and 18-d delay in heading date at the mature stage. Genetic analysis indicates that the bc88 mutant is controlled by a single recessive nuclear gene. The mutated bc88 gene isolated by map-based cloning contains only one point mutation in the 5th exon relative to its wild-type BC88 (LOC_Os09g25490 and Os09g0422500), leading to an amino acid change from P to L in bc88 plants. Alignment of the putative protein sequence with its homologs indicates that the mutation is located in the conserved region of the sequence. Detection of the transcription level of BC88 in rice plants shows that the expression level of BC88 is higher in spikes and culms than in leaves, roots, and leaf sheaths. These contribute to understanding of the molecular mechanism of cellulose synthesis. The target gene BC88 can be a useful tool in molecular marker-assisted selection for rice culm trait breeding.
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 ;The brittle culm (bc) mutants of Gramineae plants having brittle skeletal structures are valuable materials for studying secondary cell walls. In contrast to other recessive bc mutants, rice Bc6 is a semi-dominant bc mutant with easily breakable plant bodies. In this study, the Bc6 gene was cloned by positional cloning. Bc6 encodes a cellulose synthase catalytic subunit, OsCesA9, and has a missense mutation in its highly conserved region. In culms of the Bc6 mutant, the proportion of cellulose was reduced by 38%, while that of hemicellulose was increased by 34%. Introduction of the semi-dominant Bc6 mutant gene into wild-type rice significantly reduced the percentage of cellulose, causing brittle phenotypes. Transmission electron microscopy analysis revealed that Bc6 mutation reduced the cell wall thickness of sclerenchymal cells in culms. In rice expressing a reporter construct, BC6 promoter activity was detected in the culms, nodes, and flowers, and was localized primarily in xylem tissues. This expression pattern was highly similar to that of BC1, which encodes a COBRA-like protein involved in cellulose synthesis in secondary cell walls in rice. These results indicate that BC6 is a secondary cell wall-specific CesA that plays an important role in proper deposition of cellulose in the secondary cell walls.
 ===Expression===