Os09g0452200 - Revision history

Rice2012: /* Structured Information */

2015-06-12T09:45:05Z

‎Structured Information

Henikanxue at 14:57, 7 June 2014

2014-06-07T14:57:58Z

Henikanxue: /* Annotated Information */

2014-06-07T14:49:12Z

‎Annotated Information

Henikanxue: /* References */

2014-06-07T03:45:13Z

‎References

Henikanxue: /* Labs working on this gene */

2014-06-07T03:30:02Z

‎Labs working on this gene

Henikanxue: /* Annotated Information */

2014-06-07T03:26:49Z

‎Annotated Information

Henikanxue: /* Function */

2014-06-06T15:47:42Z

‎Function

Henikanxue: /* Function */

2014-06-06T14:26:55Z

‎Function

Henikanxue at 09:02, 6 June 2014

2014-06-06T09:02:43Z

Henikanxue: /* Function */

2014-06-06T09:01:07Z

‎Function

@@ Line 49: / Line 49: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 1: / Line 1: @@
-This gene is named as LYP4.
+This gene is named as LYP4. LYP4 and LYP6 are dual function receptors sensing both bacterial PGN and fungal chitin in rice innate immunity.
 ==Annotated Information==
@@ Line 44: / Line 44: @@
 [http://www.plantcell.org/content/24/8/3406 fullText]
 </ref>
 </references>

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 '''Rice LYP4 and LYP6 Are LysM-Containing Proteins Localized at the Plasma Membrane'''
 <br/>
-Bioinformatic analysis predicted that LYP4 and LYP6 both have an N-terminal signal peptide, two characteristic LysMs, and a putative C-terminal glycosylphosphatidylinositol (GPI) anchor signal sequence. All characterized homologs of LYP4 and LYP6 have been verified as plasma membrane proteins. <ref name="ref1"/>
+&nbsp;&nbsp;&nbsp;&nbsp; Bioinformatic analysis predicted that LYP4 and LYP6 both have an N-terminal signal peptide, two characteristic LysMs, and a putative C-terminal glycosylphosphatidylinositol (GPI) anchor signal sequence. All characterized homologs of LYP4 and LYP6 have been verified as plasma membrane proteins. <ref name="ref1"/>
 <br/>
 ===Expression===
-''LYP4'' and ''LYP6'' were most abundantly expressed in rice callus cells, and both transcripts progressively decreased during maturation (Figure 1D). Furthermore, analysis of ''LYP4'' and ''LYP6'' expression patterns in ''Promoter:GUS''(for b-glucuronidase) transgenic rice demonstrated strong GUS staining in young seedlings, particularly in the root meristem region and the lateral root primordium (see Supplemental Figure 2 online), resembling the expression patterns of their orthologLYM1 in ''M. truncatula''(Fliegmann et al., 2011). Interestingly, expression ofLYP4andLYP6in rice seedlings, mature leaves, and roots could be quickly induced upon exposure to the rice bacterial pathogen ''Xanthomonas oryzae'' pv ''oryzae''.<ref name="ref1"/>
+&nbsp;&nbsp;&nbsp;&nbsp; ''LYP4'' and ''LYP6'' were most abundantly expressed in rice callus cells, and both transcripts progressively decreased during maturation (Figure 1D). Furthermore, analysis of ''LYP4'' and ''LYP6'' expression patterns in ''Promoter:GUS''(for b-glucuronidase) transgenic rice demonstrated strong GUS staining in young seedlings, particularly in the root meristem region and the lateral root primordium, resembling the expression patterns of their orthologLYM1 in ''M. truncatula''(Fliegmann et al., 2011 <ref name="ref2"/>). Interestingly, expression ofLYP4andLYP6in rice seedlings, mature leaves, and roots could be quickly induced upon exposure to the rice bacterial pathogen ''Xanthomonas oryzae'' pv ''oryzae''. <ref name="ref1"/>
-----
+<br />
-[[File:Figure_1.LysM-Containing_LYP4_and_LYP6_Are_Rice_Plasma_Membrane_Proteins.png|thumb|Figure 1]]
+&nbsp;&nbsp;&nbsp;&nbsp; ''LYP4'' and ''LYP6'' expressed in rice protoplasts also could be precipitated by different bacterial PGNs and commercial chitin products (Figure 2E), confirming the physical association of PGN and chitin to LYPs in rice. By contrast, the CEBiP proteins successfully expressed in rice protoplasts could be precipitated only by chitin but not PGN, while the full-length rice CERK1 proteins expressed in rice protoplasts could be pulled down by neither MAMP (Figure 2E). <ref name="ref1"/>
-[[File:Fig_2.png|thumb|Figure 2]]
+<br/><br/>
 ===Evolution===
 Please input evolution information here.

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 ==References==
 Bing Liu;Jian-Feng Li;Ying Ao;Jinwang Qu;Zhangqun Li;Jianbin Su;Yang Zhang;Jun Liu;Dongru Feng;Kangbiao Qi;Yanming He;Jinfa Wang;Hong-Bin Wang. Lysin Motif–Containing Proteins LYP4 and LYP6 Play Dual Roles in Peptidoglycan and Chitin Perception in Rice Innate Immunity. The Plant Cell, 2012, 24(8): 3406-3419.
 [http://www.plantcell.org/content/24/8/3406 fullText]
 ==Structured Information==

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 ==Labs working on this gene==
-State Key Laboratory of Biocontrol,Key Laboratory of Gene Engineering of the Ministry of Education,
+*State Key Laboratory of Biocontrol
-Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University
+*Key Laboratory of Gene Engineering of the Ministry of Education
 ==References==

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 ===Function===
 <p>
 LYP4 and LYP6 are dual function receptors sensing both bacterial PGN and fungal chitin in rice innate
-immunity. This gene's sign is LYP4 which function is PGN and chitin oligosaccharide elicitor-binding protein.
+immunity. in vitro ligand binding assays demonstrated that these proteins could not only bind to PGN but also to chitin. Silencing of either LYPgene significantly compromised PGN- and chitin-induced defense responses in rice, leading to increased susceptibility to both bacterial and fungal pathogens. </p>
-</p>
+<br/>
 <br/>
 ===Expression===

← Older revision		Revision as of 09:02, 6 June 2014
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−	~~Please input one-sentence summary here.~~

	This gene is named as LYP4.		This gene is named as LYP4.