Os09g0470500 - Revision history

Xysj2017 at 00:39, 15 February 2017

2017-02-15T00:39:26Z

Xysj2017: /* Annotated Information */

2017-02-15T00:38:58Z

‎Annotated Information

Xysj2017: /* Mutant */

2017-02-15T00:38:14Z

‎Mutant

Xysj2017 at 00:37, 15 February 2017

2017-02-15T00:37:28Z

Rice2012: /* Structured Information */

2015-06-12T09:46:55Z

‎Structured Information

Littlescrew: /* Labs working on this gene */

2014-06-10T10:43:12Z

‎Labs working on this gene

Littlescrew: /* Mutant */

2014-06-10T10:36:58Z

‎Mutant

Littlescrew: /* Subcellular localization */

2014-06-10T10:30:17Z

‎Subcellular localization

Littlescrew: /* Expression */

2014-06-10T09:28:38Z

‎Expression

Littlescrew: /* References */

2014-06-10T08:47:23Z

‎References

@@ Line 14: / Line 14: @@
 ===Subcellular localization===
 To understand the subcellular localization of ''Oshox4'', researchers fused Oshox4 with enhanced green fluorescence protein to produce recombinant proteins at the downstream of cauliflower mosaic virus (CaMV) 35S promoter and introduced into the onion cells. The confocal microscopy pictures show that fusion protein was localized in the nuclei of the onion cells <ref name="ref4" />(Fig. 2B).
-[[File:Oshox-3.jpg|right|thumb|430px|'''Figure 5.''' Growth of ''Oshox4''-over expressing and wild type plants treated with GA3 at different concentrations. Wild type (a) and ''Oshox4''-overexpressing plants (b) at four-leaf stage were transferred to hydroponic culture media containing GA3 at the concentration of 0 (1), 0.01 (2), 0.05 (3), 0.1 (4), 0.5 (5), 1 (6), 10 (7), or 20lM (8). The lengths of the second leaf sheaths were measured 12 days after the treatment. (from reference) <ref name="ref4" />.'']]
+[[File:Oshox-3.jpg|right|thumb|327px|'''Figure 5.''' Growth of ''Oshox4''-over expressing and wild type plants treated with GA3 at different concentrations. Wild type (a) and ''Oshox4''-overexpressing plants (b) at four-leaf stage were transferred to hydroponic culture media containing GA3 at the concentration of 0 (1), 0.01 (2), 0.05 (3), 0.1 (4), 0.5 (5), 1 (6), 10 (7), or 20lM (8). The lengths of the second leaf sheaths were measured 12 days after the treatment. (from reference) <ref name="ref4" />.'']]
 ===Mutant===

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Oshox-1.jpg|right|thumb|430px|'''Figure 2B.''' Nuclear localization of Oshox4 protein. GFP alone was localized in cytosol of onion skin cells. (a)Oshox4-GFP; Nuclear localised; (c) DAPI staining; (d) a bright light image. Bar=50lm.(from reference) <ref name="ref4" />.]]
+[[File:Oshox-1.jpg|right|thumb|327x|'''Figure 2B.''' Nuclear localization of Oshox4 protein. GFP alone was localized in cytosol of onion skin cells. (a)Oshox4-GFP; Nuclear localised; (c) DAPI staining; (d) a bright light image. Bar=50lm.(from reference) <ref name="ref4" />.]]
 ===Function===
 ''Oshox4'' belongs to the homeodomain leucine zipper (HD-Zip) genes that encode transcription factors that contain conserved DNA motif called the homeobox<ref name="ref1" />. There are four family members in HD-Zip including I, I, II, III and IV <ref name="ref2" />, and Oshox4 which locates in chromosome 9 belongs to HD-Zip I. In addition, it is suggested that all HD-Zip I and II proteins family members can form homodimers and also heterodimers<ref name="ref3" />.
@@ Line 9: / Line 9: @@
 ''Oshox4'' expresses in leaf primordial of shoot apex, and in young leaves without polar distribution, even though being enhanced in upper and lower epidermis and vascular bundles surrounding cells. In addition, from floral meristems, floral organ primordia and the embryonic axis, we can detect Oshox4 mRNA. ''Oshox4'' expresses more in drought resistant than in drought-sensitive rice cultivars <ref name="ref1" />.
-[[File:Oshox-2.jpg|right|thumb|300px|'''Figure 3A.'''Over-expression of ''Oshox4'' induced a semi-dwarf phenotype in transgenic rice. (A) Comparison between wild type and an ''Oshox4'' over-expressing line (H4) at vegetative and reproductive stages. Bars=15 cm.(from reference) <ref name="ref4" />.'']]
+[[File:Oshox-2.jpg|right|thumb|327px|'''Figure 3A.'''Over-expression of ''Oshox4'' induced a semi-dwarf phenotype in transgenic rice. (A) Comparison between wild type and an ''Oshox4'' over-expressing line (H4) at vegetative and reproductive stages. Bars=15 cm.(from reference) <ref name="ref4" />.'']]
 ===Evolution===
 Please input evolution information here.

@@ Line 17: / Line 17: @@
 ===Mutant===
-When Oshox4 is over expressed in more than 40 independent transgenic T0 plants, there are 27 semi-dwarf phenotype plants that possess only a half of the wild ones as well as two times tillers than the normal wild plants (Fig. 3A).
+* When Oshox4 is over expressed in more than 40 independent transgenic T0 plants, there are 27 semi-dwarf phenotype plants that possess only a half of the wild ones as well as two times tillers than the normal wild plants (Fig. 3A).
- This may due to the reduced transgenic plants cell elongation that occurs in epidermal, elongating and intercalary meristem cells compared to wild type. And Oshox4 may interact with the fragment corresponding to -1080 to-1662 from the initiation ATG codon of the ''YAB1''. Further experiments show that these Oshox4-over-expression plants with semi-dwarf cannot be rescued by adding GA3 (Fig 5), but the mutant plants with semi-dwarf caused by YAB1-over-expression can rehabilitate to the same height of the wild type <ref name="ref5" />.
+* In Oshox4 over expressed mutants, both the GA metabolic gene expression and the amylase production change. At the same time, the GA upregulate the expression level of ''Oshox4''<ref name="ref4" />. Oshox4 knock-down plants by the expression of RNAi vector with a gene-specific segmet of  ''Oshox4'' show no significant changes, which may due to the function of other HD-Zip I in rice <ref name="ref4" />.
-In Oshox4 over expressed mutants, both the GA metabolic gene expression and the amylase production change. At the same time, the GA upregulate the expression level of ''Oshox4''<ref name="ref4" />. Oshox4 knock-down plants by the expression of RNAi vector with a gene-specific segmet of  ''Oshox4'' show no significant changes, which may due to the function of other HD-Zip I in rice <ref name="ref4" />.
+* Considering the previous reports about Arabidopsis<ref name="ref6" /> and gibberellin biosynthesis <ref name="ref7" /> and other evidences, it is predicted that Oshox4 function in GA signaling.
 ==Labs working on this gene==

@@ Line 2: / Line 2: @@
 ==Annotated Information==
 ===Function===
 ''Oshox4'' belongs to the homeodomain leucine zipper (HD-Zip) genes that encode transcription factors that contain conserved DNA motif called the homeobox<ref name="ref1" />. There are four family members in HD-Zip including I, I, II, III and IV <ref name="ref2" />, and Oshox4 which locates in chromosome 9 belongs to HD-Zip I. In addition, it is suggested that all HD-Zip I and II proteins family members can form homodimers and also heterodimers<ref name="ref3" />.
@@ Line 8: / Line 9: @@
 ''Oshox4'' expresses in leaf primordial of shoot apex, and in young leaves without polar distribution, even though being enhanced in upper and lower epidermis and vascular bundles surrounding cells. In addition, from floral meristems, floral organ primordia and the embryonic axis, we can detect Oshox4 mRNA. ''Oshox4'' expresses more in drought resistant than in drought-sensitive rice cultivars <ref name="ref1" />.
 ===Evolution===
 Please input evolution information here.
 ===Subcellular localization===
-To understand the subcellular localization of ''Oshox4'', researchers fused Oshox4 with enhanced green fluorescence protein to produce recombinant proteins at the downstream of cauliflower mosaic virus (CaMV) 35S promoter and introduced into the onion cells. The confocal microscopy pictures show that fusion protein was localized in the nuclei of the onion cells <ref name="ref4" />(Fig. 2B).[[File:Oshox-1.jpg|right|thumb|430px|'''Figure 2B.''' Nuclear localization of Oshox4 protein. GFP alone was localized in cytosol of onion skin cells. (a)Oshox4-GFP; Nuclear localised; (c) DAPI staining; (d) a bright light image. Bar=50lm.(from reference) <ref name="ref4" />.]]
+To understand the subcellular localization of ''Oshox4'', researchers fused Oshox4 with enhanced green fluorescence protein to produce recombinant proteins at the downstream of cauliflower mosaic virus (CaMV) 35S promoter and introduced into the onion cells. The confocal microscopy pictures show that fusion protein was localized in the nuclei of the onion cells <ref name="ref4" />(Fig. 2B).
 ===Mutant===
 When Oshox4 is over expressed in more than 40 independent transgenic T0 plants, there are 27 semi-dwarf phenotype plants that possess only a half of the wild ones as well as two times tillers than the normal wild plants (Fig. 3A).
   This may due to the reduced transgenic plants cell elongation that occurs in epidermal, elongating and intercalary meristem cells compared to wild type. And Oshox4 may interact with the fragment corresponding to -1080 to-1662 from the initiation ATG codon of the ''YAB1''. Further experiments show that these Oshox4-over-expression plants with semi-dwarf cannot be rescued by adding GA3 (Fig 5), but the mutant plants with semi-dwarf caused by YAB1-over-expression can rehabilitate to the same height of the wild type <ref name="ref5" />.
 In Oshox4 over expressed mutants, both the GA metabolic gene expression and the amylase production change. At the same time, the GA upregulate the expression level of ''Oshox4''<ref name="ref4" />. Oshox4 knock-down plants by the expression of RNAi vector with a gene-specific segmet of  ''Oshox4'' show no significant changes, which may due to the function of other HD-Zip I in rice <ref name="ref4" />.

@@ Line 45: / Line 45: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 26: / Line 26: @@
 ==Labs working on this gene==
-Please input related labs here.
+*National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, China
 ==References==

@@ Line 6: / Line 6: @@
 ===Expression===
-''Oshox4'' expresses in leaf primordial of shoot apex, and in young leaves without polar distribution (Fig. 2A, a,b), even though being enhanced in upper and lower epidermis and vascular bundles surrounding cells. In addition, from floral meristems, floral organ primordia and the embryonic axis, we can detect Oshox4 mRNA (Fig. 2A, c–e). ''Oshox4'' expresses more in drought resistant than in drought-sensitive rice cultivars <ref name="ref1" />.
+''Oshox4'' expresses in leaf primordial of shoot apex, and in young leaves without polar distribution, even though being enhanced in upper and lower epidermis and vascular bundles surrounding cells. In addition, from floral meristems, floral organ primordia and the embryonic axis, we can detect Oshox4 mRNA. ''Oshox4'' expresses more in drought resistant than in drought-sensitive rice cultivars <ref name="ref1" />.
 ===Evolution===

@@ Line 23: / Line 23: @@
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==