Os10g0127900 - Revision history

Xysj1981 at 06:32, 8 March 2017

2017-03-08T06:32:30Z

Rice2012: /* References */

2015-06-13T08:43:35Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T03:26:53Z

‎Structured Information

Sunnymi: /* Expression */

2014-06-08T16:19:32Z

‎Expression

Sunnymi: /* Expression */

2014-06-08T16:16:33Z

‎Expression

Sunnymi: /* Expression */

2014-06-08T16:12:42Z

‎Expression

Jieomlong: /* Expression */

2014-06-08T15:01:36Z

‎Expression

Jieomlong: /* Expression */

2014-06-08T15:00:49Z

‎Expression

Jieomlong: /* Expression */

2014-06-08T15:00:03Z

‎Expression

Jieomlong: /* Expression */

2014-06-08T14:58:42Z

‎Expression

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 ===Expression===
 .OsFbx352 encodes a F-box domain protein and is suppressed by glucose
-A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]
+A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.
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 To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed
 by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.
-[[File:2.jpg]]
 Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.
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 germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA
 on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the
-presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]
+presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.
 Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P < 0.05; **, P < 0.01 (this figure is available in colour at JXB online).
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 effect. These results suggest that overexpression of OsFbx352 disrupts glucose-dependent ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.
-[[File:5.jpg]]
 Fig. 4. Effects of water, mannitol, and glucose treatments on endogenous ABA contents in germinating seeds. Seeds were treated with water, mannitol, or glucose for 6 h at 28 °C and were subsequently collected for ABA quantification. Data are mean ± SE with three replicates. Data were statistically analysed with t-test. Statistically significant difference from the wild type within a treatment: *, P < 0.05; **, P < 0.01.

← Older revision		Revision as of 08:43, 13 June 2015
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	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	== Structured Information ==

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 . Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 To investigate whether the altered sensitivity of seed germination in the OsFbx352-overexpressing and RNAi lines to glucose is related to ABA, changes in ABA contents in seeds of WT, overexpressing and RNAi lines were monitored during imbibition in the absence and presence of glucose. There were no differences in ABA contents in dry seeds among WT, overexpressing, and RNAi lines (Fig. 4). ABA contents in WT, overexpressing, and RNAi seeds were sharply reduced upon imbibition (Fig. 4). Moreover, ABA contents in the imbibed seeds were increased when glucose was present in the incubation medium, and the glucose-induced increase in ABA contents in the overexpressing seeds was significantly lower than in WT seeds, while ABA contents in the RNAi seeds were significantly higher than in WT seeds in the presence of glucose (Fig. 4). In contrast to glucose, addition of the same concentration of mannitol to the incubation medium had no effect on ABA levels in seeds of both WT and transgenic lines (Fig. 4）, discounting the possibility that the glucose-induced increases in ABA levels in seeds are due to osmotic
 effect. These results suggest that overexpression of OsFbx352 disrupts glucose-dependent ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.
 [[File:5.jpg]]
 Fig. 4. Effects of water, mannitol, and glucose treatments on endogenous ABA contents in germinating seeds. Seeds were treated with water, mannitol, or glucose for 6 h at 28 °C and were subsequently collected for ABA quantification. Data are mean ± SE with three replicates. Data were statistically analysed with t-test. Statistically significant difference from the wild type within a treatment: *, P < 0.05; **, P < 0.01.

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 Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P < 0.05; **, P < 0.01 (this figure is available in colour at JXB online).
 .Overexpression of OsFbx352 suppressed glucose-induced increase in seed ABA content
 To investigate whether the altered sensitivity of seed germination in the OsFbx352-overexpressing and RNAi lines to glucose is related to ABA, changes in ABA contents in seeds of WT, overexpressing and RNAi lines were monitored during imbibition in the absence and presence of glucose. There were no differences in ABA contents in dry seeds among WT, overexpressing, and RNAi lines (Fig. 4). ABA contents in WT, overexpressing, and RNAi seeds were sharply reduced upon imbibition (Fig. 4). Moreover, ABA contents in the imbibed seeds were increased when glucose was present in the incubation medium, and the glucose-induced increase in ABA contents in the overexpressing seeds was significantly lower than in WT seeds, while ABA contents in the RNAi seeds were significantly higher than in WT seeds in the presence of glucose (Fig. 4). In contrast to glucose, addition of the same concentration of mannitol to the incubation medium had no effect on ABA levels in seeds of both WT and transgenic lines (Fig. 4）, discounting the possibility that the glucose-induced increases in ABA levels in seeds are due to osmotic
 effect. These results suggest that overexpression of OsFbx352 disrupts glucose-dependent ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.
 ===Evolution===

← Older revision		Revision as of 16:12, 8 June 2014
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	Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: , P < 0.05; *, P < 0.01 (this figure is available in colour at JXB online).		Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: , P < 0.05; *, P < 0.01 (this figure is available in colour at JXB online).
		+	4.Overexpression of OsFbx352 suppressed glucose-induced increase in seed ABA content
		+	To investigate whether the altered sensitivity of seed germination in the OsFbx352-overexpressing and RNAi lines to glucose is related to ABA, changes in ABA contents in seeds of WT, overexpressing and RNAi lines were monitored during imbibition in the absence and presence of glucose. There were no differences in ABA contents in dry seeds among WT, overexpressing, and RNAi lines (Fig. 4). ABA contents in WT, overexpressing, and RNAi seeds were sharply reduced upon imbibition (Fig. 4). Moreover, ABA contents in the imbibed seeds were increased when glucose was present in the incubation medium, and the glucose-induced increase in ABA contents in the overexpressing seeds was significantly lower than in WT seeds, while ABA contents in the RNAi seeds were significantly higher than in WT seeds in the presence of glucose (Fig. 4). In contrast to glucose, addition of the same concentration of mannitol to the incubation medium had no effect on ABA levels in seeds of both WT and transgenic lines (Fig. 4）, discounting the possibility that the glucose-induced increases in ABA levels in seeds are due to osmotic
		+	effect. These results suggest that overexpression of OsFbx352 disrupts glucose-dependent ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.

	===Evolution===		===Evolution===