Os10g0496900 - Revision history

Rice2012: /* References */

2015-06-13T08:47:06Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:03:05Z

‎Structured Information

Yueliya: /* Evolution */

2014-06-10T09:44:23Z

‎Evolution

Yueliya: /* Evolution */

2014-06-10T09:21:59Z

‎Evolution

Yueliya: /* Evolution */

2014-06-10T09:19:35Z

‎Evolution

Yueliya: /* Expression */

2014-06-10T09:18:53Z

‎Expression

Yueliya: /* Expression */

2014-06-10T09:18:12Z

‎Expression

Yueliya: /* Function */

2014-06-10T09:16:07Z

‎Function

Yueliya: /* Function */

2014-06-10T09:15:20Z

‎Function

Yueliya: /* Function */

2014-06-10T09:14:38Z

‎Function

← Older revision		Revision as of 08:47, 13 June 2015
Line 88:		Line 88:
	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	== Structured Information ==

@@ Line 82: / Line 82: @@
 , 44, 963–974.
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 61: / Line 61: @@
 ===Evolution===
-There are two rice POR isoforms and three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana. The following is the phylogenetic tree of PORs in Arabidopsis, rice and barley.
+There are two rice POR isoforms and three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana. Both Arabidopsis and rice ''PORB'' genes are constitutively expressed throughout development, but their responses to light intensity were somewhat different. ''OsPORB'' expression was rapidly upregulated by HL treatment, similar to the expression of ''AtPORC''. In summary, Sakuraba et al propose that ''OsPORA'' has overlapping functions corresponding to ''AtPORA'' and ''AtPORB'', but that ''OsPORB'' has functions corresponding to ''AtPORB'' and ''AtPORC''. In agreement with this hypothesis, a phylogenic tree of Arabidopsis, rice and barley PORs suggests that OsPORA and HvPORA are evolutionarily closer to AtPORA and AtPORB, but that OsPORB and HvPORB are similar to AtPORC.
 [[File:figure S7 yue.jpg|500px|center|Figure 4. Phylogenetic tree of PORs in Arabidopsis, rice and barley.]]

@@ Line 61: / Line 61: @@
 ===Evolution===
-NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the light-dependent reduction of protochlorophyllide. There are two rice POR isoforms and three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana. The following is the phylogenetic tree of PORs in Arabidopsis, rice and barley.
+There are two rice POR isoforms and three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana. The following is the phylogenetic tree of PORs in Arabidopsis, rice and barley.
 [[File:figure S7 yue.jpg|500px|center|Figure 4. Phylogenetic tree of PORs in Arabidopsis, rice and barley.]]

@@ Line 63: / Line 63: @@
 NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the light-dependent reduction of protochlorophyllide. There are two rice POR isoforms and three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana. The following is the phylogenetic tree of PORs in Arabidopsis, rice and barley.
-[[File:figure S7 yue.jpg|500px|center|Figure 3. Phylogenetic tree of PORs in Arabidopsis, rice and barley.]]
+[[File:figure S7 yue.jpg|500px|center|Figure 4. Phylogenetic tree of PORs in Arabidopsis, rice and barley.]]
 ==Labs working on this gene==

← Older revision		Revision as of 09:18, 10 June 2014
Line 42:		Line 42:


−	'''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure5-1y.png\|350px\|Figure 2. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]][[File:figure5-2y.png\|300px\|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]]	+	'''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure5-1y.png\|350px\|Figure 2. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]][[File:figure5-2y.png\|300px\|Figure 2. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]]

	First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure 2a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 2b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in ''OsPORB''.		First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure 2a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 2b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in ''OsPORB''.

@@ Line 42: / Line 42: @@
-'''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure5-1y.png|350px|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]][[File:figure5-2y.png|300px|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]]
+'''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure5-1y.png|350px|Figure 2. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]][[File:figure5-2y.png|300px|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]]
-First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure 1a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 1b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in ''OsPORB''.
+First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure 2a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 2b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in ''OsPORB''.
-Next, to examine whether OsPORA contributes to light-dependent Chl synthesis during leaf greening, Sakuraba et al compared the greening speed of wild-type and fgl seedlings. The visible speed of greening (Figure 1c) and the rates of Chl synthesis (Figure 1d) were not significantly altered in fgl, indicating that low levels of OsPORA are sufficient for leaf greening in rice, even in the absence of OsPORB activity in fgl mutants.
+Next, to examine whether OsPORA contributes to light-dependent Chl synthesis during leaf greening, Sakuraba et al compared the greening speed of wild-type and fgl seedlings. The visible speed of greening (Figure 2c) and the rates of Chl synthesis (Figure 2d) were not significantly altered in ''fgl'', indicating that low levels of OsPORA are sufficient for leaf greening in rice, even in the absence of OsPORB activity in ''fgl'' mutants.
 '''Enhanced ''OsPORB'' activity is essential for Chl synthesis under HL.'''
-[[File:figure 7 yue.png|left|400px|Figure 2. Effect of high light(HL) on Chl synthesis and ''OsPOR'' expression.]]
+[[File:figure 7 yue.png|left|400px|Figure 3. Effect of high light(HL) on Chl synthesis and ''OsPOR'' expression.]]
 In Arabidopsis, ''AtPORC'' expression increases under HL conditions, whereas both ''AtPORA'' and ''AtPORB'' are downregulated (Masuda et al., 2003), indicating that ''AtPORC'' is responsible for Chl synthesis under HL conditions. Thus, Sakuraba et al examined whether ''OsPORB'' has a similar function to ''AtPORC'' for Chl synthesis in response to HL.
-The 14–day-old wild-type and fgl seedlings grown under mild light intensity (200 μmol m−2 s−1) were transferred to HL (1500 μmol m−2 s−1) for 3 days in SD conditions (Figure 2). Compared with the wild type, fgl exhibited a severely photobleached phenotype (Figure 2a,b), possibly as a result of photooxidative damage. In the wild type, ''OsPORA'' mRNA levels were lower after 3 h of HL treatment (Figure 2c), whereas ''OsPORB'' mRNA levels drastically increased (Figure 2d), indicating that the rice plant requires increased expression of ''OsPORB'' in response to HL conditions. The levels of OsPORA and OsPORB were consistent with their mRNA levels (Figure 2e), indicating that rice POR activity is regulated at the transcriptional level.
+The 14–day-old wild-type and fgl seedlings grown under mild light intensity (200 μmol m−2 s−1) were transferred to HL (1500 μmol m−2 s−1) for 3 days in SD conditions (Figure 3). Compared with the wild type, fgl exhibited a severely photobleached phenotype (Figure 3a,b), possibly as a result of photooxidative damage. In the wild type, ''OsPORA'' mRNA levels were lower after 3 h of HL treatment (Figure 3c), whereas ''OsPORB'' mRNA levels drastically increased (Figure 3d), indicating that the rice plant requires increased expression of ''OsPORB'' in response to HL conditions. The levels of OsPORA and OsPORB were consistent with their mRNA levels (Figure 3e), indicating that rice POR activity is regulated at the transcriptional level.
 These results strongly suggest that OsPORB activity is necessary for Chl synthesis under HL conditions in the paddy field, reminiscent of AtPORC function in Arabidopsis (Masuda et al., 2003).

← Older revision		Revision as of 09:16, 10 June 2014
Line 29:		Line 29:

	(e) dCAPS analysis was performed to confirm the 1-bp deletion in ''OsPORB''. AatII digests the genomic PCR products from the ''fgl'' mutant (lane 1) but not from the wild type ‘Kinmaze’ (lane 2). Lane 3, a mapping parent ‘Dasanbyeo’; lane 4, F1hybrid (Dasanbyeo/fgl).		(e) dCAPS analysis was performed to confirm the 1-bp deletion in ''OsPORB''. AatII digests the genomic PCR products from the ''fgl'' mutant (lane 1) but not from the wild type ‘Kinmaze’ (lane 2). Lane 3, a mapping parent ‘Dasanbyeo’; lane 4, F1hybrid (Dasanbyeo/fgl).
−

← Older revision		Revision as of 09:15, 10 June 2014
Line 29:		Line 29:

	(e) dCAPS analysis was performed to confirm the 1-bp deletion in ''OsPORB''. AatII digests the genomic PCR products from the ''fgl'' mutant (lane 1) but not from the wild type ‘Kinmaze’ (lane 2). Lane 3, a mapping parent ‘Dasanbyeo’; lane 4, F1hybrid (Dasanbyeo/fgl).		(e) dCAPS analysis was performed to confirm the 1-bp deletion in ''OsPORB''. AatII digests the genomic PCR products from the ''fgl'' mutant (lane 1) but not from the wild type ‘Kinmaze’ (lane 2). Lane 3, a mapping parent ‘Dasanbyeo’; lane 4, F1hybrid (Dasanbyeo/fgl).
−

@@ Line 12: / Line 12: @@
 [[File:figure 0y.png|350px|left|Figure 1. Map-based cloning of the ''fgl'' locus.]]
@@ Line 25: / Line 26: @@
 (c) Candidate genes (black boxes) in the 47–kb genomic DNA.
-(d) A frameshift mutation of ''OsPORB'' in the fgl mutant. Four exons and three introns are indicated by rectangles and thick lines, respectively. The position of the 1-bp deletion (G109) is marked in the second exon.
+(d) A frameshift mutation of ''OsPORB'' in the fgl mutant. Four exons and three introns are indicated by rectangles and thick lines, respectively. The  position of the 1-bp deletion (G109) is marked in the second exon.
 (e) dCAPS analysis was performed to confirm the 1-bp deletion in ''OsPORB''. AatII digests the genomic PCR products from the ''fgl'' mutant (lane 1) but not from the wild type ‘Kinmaze’ (lane 2). Lane 3, a mapping parent ‘Dasanbyeo’; lane 4, F1hybrid (Dasanbyeo/fgl).