Os10g0536100 - Revision history

Xysj2018 at 01:34, 17 February 2017

2017-02-17T01:34:37Z

2017-02-17T01:33:58Z

‎References

2017-02-17T01:33:02Z

‎Labs working on this gene

2017-02-17T01:32:15Z

2017-02-17T01:30:36Z

2015-06-13T08:52:41Z

‎References

2015-06-12T04:05:55Z

‎Structured Information

2014-06-10T03:45:51Z

‎Expression

2014-06-10T03:38:55Z

‎Knowledge Extension

2014-06-10T03:34:57Z

‎Knowledge Extension

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 ===Function===
 * '''''OsMADS50''''' and '''''OsMADS56''''' may form a complex that regulates down- stream target genes.
 ===Phenotypic analysis===
 * Overexpression of OsMADS56 (56OX) resulted in delayed flowering under LD.
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 * Yeast two-hybrid and co-immunoprecipitation analyses indicated an interaction between those two proteins as well as their formation of homodimers.
 You can also add sub-section(s) at will.
 ==Labs working on this gene==

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 ==References==
-. Choong-Hwan Ryu;Shinyoung Lee;Lae-Hyeon Cho;Song Lim Kim;Yang-Seok Lee;Sang Chul Choi;Hee Joong Jeong;Jakyung Yi;Soon Ju Park;Chang-Deok Han;Gynheung An OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)-dependent flowering in rice Plant, Cell & Environment, 2009, 32(10): 1412-1427
+<references>
-. Jongmin Nam;Kerstin Kaufmann;Günter Theißen;Masatoshi Nei A simple method for predicting the functional differentiation of duplicate genes and its application to MIKC-type MADS-box genes Nucleic Acids Research, 2005, 33(2): e12
+</ref>
 [[Category:Genes]]
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 [[Category:Japonica Chromosome 10]]
 [[Category:Chromosome 10]]
 ==Structured Information==

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 ==Labs working on this gene==
-.CHOONG-HWAN RYU1 ,Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
+* CHOONG-HWAN RYU1 ,Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
-.SOON JU PARK2&CHANG-DEOK HAN, Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 660-701, Korea
+* SOON JU PARK2&CHANG-DEOK HAN, Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 660-701, Korea
 ==References==

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 ==Annotated Information==
 ===Gene Symbol===
 *'''''Os10g0536100''''' '''''<=>''''' '''''OsMADS56''''','''''MADS56,RMADS214'''''
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 * These observations imply that OsMADS50 and OsMADS56 function antagonistically through OsLFL1-Ehd1 in regulating LD-dependent flowering.
 * Yeast two-hybrid and co-immunoprecipitation analyses indicated an interaction between those two proteins as well as their formation of homodimers.
-                        [[File:fig2a.jpg]]
 ===Expression===

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-Please input one-sentence summary here.
+The rice '''''Os10g0536100''''' was reported as '''''OsMADS56''''' in 2009 <ref name="ref1" /> by researchers from Korea.
 ==Annotated Information==
 ===Function===
-OsMADS50 and OsMADS56 , which is highly homologous to Arabidopsis SOC1, are affected by the circadian clock.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a. OsMADS50 is a flowering activator that functions only under LD conditions. But OsMADS50 and OsMADS56 function antagonistically in regulating LD-dependent flowering by controlling expression of OsLFL1 and Ehd1. So OsMADS56 is an LD-specific floral repressor.OsMADS50 and OsMADS56 form a complex that regulates downstream target genes in order to regulate the Rice's flowering time.
+* '''''OsMADS50''''' and '''''OsMADS56''''' may form a complex that regulates down- stream target genes.
-OsMADS56 is highly homologous to OsMADS50. OsMADS56 is located on chromosome 10, and consists of eight exons that encode a typical MIKC-type MADS-box protein (Fig. 2a). When their full-length protein sequences are aligned, this MADS-box protein shares 60.8% homology with OsMADS50. This implies that OsMADS56 may also regulate flowering time.
+===Phenotypic analysis===
                          [[File:fig2a.jpg]]

← Older revision		Revision as of 08:52, 13 June 2015
Line 59:		Line 59:
	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	==Structured Information==

@@ Line 53: / Line 53: @@
 .Lee S., Kim J., Han J.J., Han M.J. & An G. (2004) Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice. ThePlant Journal 38, 754–764.
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 (2)Real-time RT-PCR analyses indicates that OsMADS56 transcript levels were low at the seedling stage but gradually increased to a maximum at 4 weeks after
 germination as the observation made by CHOONG-HWAN RYU1 etc. (fig.3b)
 (3)OsMADS56 and OsMADS50 control the Rice's flowering time through regulating their communal downstream genes OsLFL1-Ehd1 .The expression patterns of Ehd1, Hd3a RFT1 and OsLFL1 reveal it.(fig3c-f)
 (4)The mRNA levels of OsMADS50 and OsMADS56 along with other flowering regulators such as Ehd1, Hd1 reveals that OsMADS50 and OsMADS56 also have daily rhythms.(fig9) The transcript levels of them is similar to that of Ehd1.
 (5)OsMADS56 is located on chromosome 10, and consists of eight exons that encode a typical MIKC-type MADS-box protein.
    [[File:f3b-f.jpg]][[File:fig9.jpg]]

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 === Knowledge Extension ===
 By conducting Co-IP experiments, they confirm the physical interaction between OsMADS50 and OsMADS56. Myc-tagged OsMADS50 was first co-transfected with HA-tagged OsMADS56 into rice protoplasts before whole-protoplast extracts expressing epitope-tagged proteins were immunoprecipitated with anti-Myc antibodies. Immunoblot analysis with anti-HA antibodies revealed that HA-tagged OsMADS56 was co-precipitated with the Myc-tagged OsMADS50 (Fig. 8b). Similarly, HA-tagged OsMADS50 was co-precipitated with the Myc-tagged OsMADS56 (Fig. 8b). However, neither OsMADS50-HA nor OsMADS56-Myc alone showed such co-precipitation.These experiments supported our findings from the yeast two-hybrid trials that OsMADS50 and OsMADS56 do interact with each other. We also showed that OsMADS50 and OsMADS56 form homodimers, based on those yeast-two hybrid (data not shown) and Co-IP experiments (Fig. 8c).
-[[File:fig8a.jpg]] [[File.fig8bc.jpg]]
+[[File:fig8a.jpg]] [[File:fig8bc.jpg]]
-You can also add sub-section(s) at will.
+ You can also add sub-section(s) at will.
 ==Labs working on this gene==