Os10g0558900 - Revision history

Xysj2028: /* Annotated Information */

2017-05-08T09:13:57Z

‎Annotated Information

Xysj2028: /* Annotated Information */

2017-05-08T09:13:29Z

‎Annotated Information

Xysj2028 at 09:13, 8 May 2017

2017-05-08T09:13:08Z

Xysj2028 at 09:12, 8 May 2017

2017-05-08T09:12:20Z

Xysj2028 at 09:11, 8 May 2017

2017-05-08T09:11:36Z

Xysj1981 at 06:20, 8 March 2017

2017-03-08T06:20:27Z

Rice2012: /* References */

2015-06-13T08:57:36Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:15:24Z

‎Structured Information

EmmaJune: /* References */

2014-06-10T02:46:52Z

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Somnussunshine: /* References */

2014-06-10T02:29:40Z

‎References

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Os10g0558900.png|right|thumb|327px|'''''Figure 2.''''']]
+[[File:Os10g0558900.png|right|thumb|327px|'''''Figure 2.  Phenotypic analysis of RL14 mutants''''']]
 ===Gene Symbol===
 *'''''Os10g0558900''''' '''''<=>''''' '''''RL14,OsRL14'''''

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Os10g0558900.png|right|thumb|327px|]]
+[[File:Os10g0558900.png|right|thumb|327px|'''''Figure 2.''''']]
 ===Gene Symbol===
 *'''''Os10g0558900''''' '''''<=>''''' '''''RL14,OsRL14'''''

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 [[File:Os10g0558900.png|right|thumb|327px|]]
 ===Function===
 * '''''RL14''''' encode a 2OG-Fe (II) oxygenase of unknown function.

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Os10g0558900.png|right|thumb|327px|'''Figure 2.'''<ref name="ref1" />.'']]
+[[File:Os10g0558900.png|right|thumb|327px|]]
 ===Function===
 * '''''RL14''''' encode a 2OG-Fe (II) oxygenase of unknown function.
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 * The RL14 gene was mapped first to an interval between the simple sequence repeat (SSR) markers RM3123 and RM1162 on the long arm of chromosome 10.
 * Finally, the RL14 locus was mapped to a 24.5-kb DNA region between the InDel marker SID2 and SSR marker SW24 on a single bacterial artificial chromosome, AC079874.
-* RL14 was related most closely to members of the 2OG-Fe (II) oxygenase family from rice and sorghum (Figure 2b). Orthologous of RL14 was identified in other species, such as maize, Arabidopsis, Papaver somniferum, Vitis spp and Ricinus communis (Figure 2b).
+* RL14 was related most closely to members of the 2OG-Fe (II) oxygenase family from rice and sorghum.
 ==Labs working on this gene==

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-Please input one-sentence summary here.
+The rice '''''Os10g0558900''''' was reported as '''''RL14''''' in 2012 <ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Function===
-As an important agronomic trait, leaf rolling in rice (Oryza sativa L.) has attracted much attention from plant biologists and breeders. Moderate leaf rolling increases the amount of photosynthesis in cultivars and hence raises grain yield.
+===Phenotypic analysis===
-RL14 (rolling leaf 14) regulates leaf rolling, which is found to encode a 2OG-Fe (II) oxygenase of unknown function. the RL14 gene affects water transport in leaves by affecting the composition of the secondary cell wall. This change in water transport results in water deficiency, which is the major reason for the abnormal shape of the bulliform cells.
+* rl14 mutant plants had incurved leaves because of the shrinkage of bulliform cells on the adaxial side.
-RL14 mutant plants displayed smaller stomatal complexes and decreased transpiration rates, as compared with the wild type. Defective development could be rescued functionally by the expression of wild-type RL14.
+* In addition, rl14 mutant plants displayed smaller stomatal complexes and decreased transpiration rates, as compared with the wild type. Defective development could be rescued functionally by the expression of wild-type RL14.
 ===Expression===
+* '''''RL14''''' was transcribed in sclerenchymatous cells in leaves that remained wrapped inside the sheath.
-Histochemistry, as well as qRT-PCR analysis, showed that RL14 was highly transcribed in leaves, leaf sheaths and roots. It was also detected in stems and glumes, although its expression in these organs was not as intense as that in the mature leaves, leaf sheaths and roots (Figure 1b).
+* In mature leaves, '''''RL14''''' accumulated mainly in the mesophyll cells that surround the vasculature.
-Expression of RL14 mRNA was detected in the sclerenchymal cell layer of underemerged leaves and sheaths. In the mature leaf, RL14 mRNA accumulated mainly in the mesophyll cells that surrounded the vasculature (Figure 1c).Independent transgenic lines were identified through qRT-PCR analysis of RL14 expression using primers that matched sequences in the coding region. The results showed that the expression of RL14 was restored to the WT level in the transgenic plants.Comparison with the corresponding genomic sequence revealed that the RL14 gene consisted of three exons and two introns, and the coding sequence was 834-bp long and encoded a protein of 278 amino acids (Figure 2a). Protein sequence analysis revealed that the RL14 protein had a 2OG-Fe(II) oxygenase domain(amino acids 128–228).
+* Expression of genes related to secondary cell wall formation was affected in rl14-1 mutants, and cellulose and lignin content were altered in rl14-1 leaves.
 ===Evolution===
-The RL14 gene was mapped first to an interval between the simple sequence repeat (SSR) markers RM3123 and RM1162 on the long arm of chromosome 10.
+==Labs working on this gene==
-Finally, the RL14 locus was mapped to a 24.5-kb DNA region between the InDel marker SID2 and SSR marker SW24 on a single bacterial artificial chromosome, AC079874.
+* Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, China
 ==References==
-Fang L, Zhao F, Cong Y, et al. Rolling‐leaf14 is a 2OG‐Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves[J]. Plant biotechnology journal, 2012, 10(5): 524-532.
+<references>
 [[Category:Genes]]

← Older revision		Revision as of 08:57, 13 June 2015
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	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	==Structured Information==

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 Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, China.
 ==References==
-Likui Fang,Fangming Zhao,Yunfei Cong,et al.Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves.Plant Biotechnology Journal, 2012, 10(5): 524-532.
+Fang L, Zhao F, Cong Y, et al. Rolling‐leaf14 is a 2OG‐Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves[J]. Plant biotechnology journal, 2012, 10(5): 524-532.
 ==Structured Information==