Os10g0567400 - Revision history

Rice2012: /* References */

2015-06-13T08:58:12Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:16:37Z

‎Structured Information

Mr corn: /* Evolution */

2014-06-01T08:11:07Z

‎Evolution

Mr corn: /* Expression */

2014-06-01T08:10:45Z

‎Expression

Mr corn at 08:09, 1 June 2014

2014-06-01T08:09:54Z

Mr corn: /* Annotated Information */

2014-06-01T08:08:50Z

‎Annotated Information

Mr corn at 08:04, 1 June 2014

2014-06-01T08:04:10Z

Mr corn: /* Expression */

2014-06-01T08:00:02Z

‎Expression

Mr corn: /* Function */

2014-06-01T07:59:25Z

‎Function

Mr corn at 07:58, 1 June 2014

2014-06-01T07:58:41Z

← Older revision		Revision as of 08:58, 13 June 2015
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	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	==Structured Information==

@@ Line 79: / Line 79: @@
 [1] Sichul Lee,et.al,''Differential regulation of chlorophyll a oxygenase genes in rice'',Plant Molecular Biology (2005),57:805–818
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 67: / Line 67: @@
 ===Evolution===
-This class of proteins (chlorophyll a oxygenase (CAO) protein) they encode contain two conserved functional motifs – the Rieske Fe–sulfur coordinating center and a non-heme mononuclear Fe-binding site.
+This class of proteins (chlorophyll a oxygenase (CAO) protein) they encode contain two conserved functional motifs – the Rieske Fe–sulfur coordinating center and a non-heme mononuclear Fe-binding site<sup>[1]</sup>.
 [[File:Phylogeneic trees of the proteins.jpg|Figure 10.Phylogeneic trees of the proteins containing the conserved Rieske [2Fe–2S] binding site from ''rice'' and ''Arabidopsis'']]

@@ Line 26: / Line 26: @@
 ===Expression===
-''OsCAO1'' is induced by light and is preferentially expressed in photosynthetic tissues.''OsCAO1'' was expressed strongly in shoots and leaves[1].
+''OsCAO1'' is induced by light and is preferentially expressed in photosynthetic tissues.''OsCAO1'' was expressed strongly in shoots and leaves<sup>[1]</sup>.
 ----

@@ Line 1: / Line 1: @@
-This gene locus named after ''OsCAO1'' of Oryza sativa Japonica Group, whose length is 2983 bps and 541 amino acids ,plays a major role in chlorophyll b biosynthesis[1]
+This gene locus named after ''OsCAO1'' of Oryza sativa Japonica Group, whose length is 2983 bps and 541 amino acids ,plays a major role in chlorophyll b biosynthesis.
 ==Annotated Information==
@@ Line 17: / Line 17: @@
 * Line 1C-039-43, in which T-DNA insertion occurred in the first intron of ''OsCAO1''; the direction of the gus reporter gene was the same.Genotyping our 30
-T2 progeny resulted in the isolation of 4 homozygous(Lanes 7 , 12, 18 and 27), 17 heterozygous(1, 3, 5, 6, 10, 11, 14, 15, 17, 19, 20, 21 23, 24, 25, 26, and 28, and 9), wild-type (Lanes 2, 4, 8, 9, 13, 16, 22, 29, and 30) segregating plants[1].
+T2 progeny resulted in the isolation of 4 homozygous(Lanes 7 , 12, 18 and 27), 17 heterozygous(1, 3, 5, 6, 10, 11, 14, 15, 17, 19, 20, 21 23, 24, 25, 26, and 28, and 9), wild-type (Lanes 2, 4, 8, 9, 13, 16, 22, 29, and 30) segregating plants<sup>[1]</sup>.
 [[File:Genotyping and GUS activity in progeny of Line 1C-039-43.jpg|Figure 3.Genotyping and GUS activity in progeny of Line 1C-039-43. Thirty plants were genotyped with primers a and b (upper), or primers a and RB (lower). The 0.6-kb genomicDNAbands are PCR product using primers a and b, if no T-DNA insertion; 0.4-kb bands are PCR products using primers a and RB, if T-DNA was inserted in first intron]]
-* Line 1B-044-10, in which the Tos17 was inserted into the fourth exon of ''OsCAO1''.In genotyping its T2 progeny, we isolated three homozygous plants, all of which showed the phenotype of pale green leaves, stunted growth, and less tillering[1].
+* Line 1B-044-10, in which the Tos17 was inserted into the fourth exon of ''OsCAO1''.In genotyping its T2 progeny, we isolated three homozygous plants, all of which showed the phenotype of pale green leaves, stunted growth, and less tillering<sup>[1]</sup>.
 [[File:Genotyping of T2 progeny of Line 1B-044-10.jpg|Figure 4.(B), ND1064 (C) and 1B-025-43 (D). LTR2 is the 3' region-specific primer of Tos17.]]
@@ Line 29: / Line 29: @@
 ----
-'''The expression pattern of ''OsCAO1'' was light-dependent'''[1]
+'''The expression pattern of ''OsCAO1'' was light-dependent'''<sup>[1]</sup>
 During the reproductive development stages, its expression level was higher in mature than in immature panicles.
@@ Line 41: / Line 41: @@
 ----
-'''Transcript levels of ''OsCAO1'' followed a circadian rhythm'''[1]
+'''Transcript levels of ''OsCAO1'' followed a circadian rhythm'''<sup>[1]</sup>
 Because the expression pattern of ''OsCAO1'' was light-dependent, we examined whether its transcript level followed a circadian rhythm.Transcript levels
@@ Line 77: / Line 77: @@
 ==References==
-[1] [[1]]Sichul Lee,et.al,''Differential regulation of chlorophyll a oxygenase genes in rice'',Plant Molecular Biology (2005),57:805–818
+[1] Sichul Lee,et.al,''Differential regulation of chlorophyll a oxygenase genes in rice'',Plant Molecular Biology (2005),57:805–818
 ==Structured Information==

@@ Line 4: / Line 4: @@
 ===Function===
-''OsCAO1'' plays a major role in chlorophyll b biosynthesis.Knockout mutant lines tagged by T-DNA or Tos17 have pale green leaves, indicating chlorophyll b deficiency.And the manifested phenotype was one of pale green leaves and retarded growth,besides those particular plants produced fewer tillers, usually 3–5 compared with 13–15 in the wild types[1].
+''OsCAO1'' plays a major role in chlorophyll b biosynthesis.Knockout mutant lines tagged by T-DNA or Tos17 have pale green leaves, indicating chlorophyll b deficiency.And the manifested phenotype was one of pale green leaves and retarded growth,besides those particular plants produced fewer tillers, usually 3–5 compared with 13–15 in the wild types<sup>[1]</sup>.
 [[File:Phenotype comparisons between OsCAO1 KO and WT.jpg|Figure 1.Phenotype comparisons between ''OsCAO1'' KO and WT plants at 30 DAG. T/T, homozygous mutant plant; W/W, wild-type segregant from tagged line; W/T, heterozygous plant.]]
@@ Line 70: / Line 70: @@
 [[File:Phylogeneic trees of the proteins.jpg|Figure 10.Phylogeneic trees of the proteins containing the conserved Rieske [2Fe–2S] binding site from ''rice'' and ''Arabidopsis'']]
 ==Labs working on this gene==

@@ Line 50: / Line 50: @@
 ''OsCAO1'' was inducible by all light treatments, but most significantly under blue light.
-[[File:Analysis of ''OsCAO1'' under different light conditions.jpg|Figure 9.Expression levels were determined in 4-day-old dark-grown wild-type seedlings after exposure to various light sources. D, darkness; Fr, far-red light; R, red light; B, blue light; W, white light.]]
+[[File:Analysis of OsCAO1 under different light conditions.jpg|Figure 9.Expression levels were determined in 4-day-old dark-grown wild-type seedlings after exposure to various light sources. D, darkness; Fr, far-red light; R, red light; B, blue light; W, white light.]]
 ----
 {| class="wikitable"

@@ Line 14: / Line 14: @@
 The schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17''.
-[[File:Schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17''.jpg|Figure 2.Schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17'']]
+[[File:Schematic diagrams of the rice CAO genes and insertion positions of T-DNA and Tos17.jpg|Figure 2.Schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17'']]
 * Line 1C-039-43, in which T-DNA insertion occurred in the first intron of ''OsCAO1''; the direction of the gus reporter gene was the same.Genotyping our 30

@@ Line 6: / Line 6: @@
 ''OsCAO1'' plays a major role in chlorophyll b biosynthesis.Knockout mutant lines tagged by T-DNA or Tos17 have pale green leaves, indicating chlorophyll b deficiency.And the manifested phenotype was one of pale green leaves and retarded growth,besides those particular plants produced fewer tillers, usually 3–5 compared with 13–15 in the wild types.
-[[File:Phenotype comparisons between OsCAO1 KO and WT.jpg|Phenotype comparisons between OsCAO1 KO and WT plants at 30 DAG. T/T, homozygous mutant plant; W/W, wild-type segregant from tagged line; W/T, heterozygous plant.]]
+[[File:Phenotype comparisons between OsCAO1 KO and WT.jpg|Figure 1.Phenotype comparisons between ''OsCAO1'' KO and WT plants at 30 DAG. T/T, homozygous mutant plant; W/W, wild-type segregant from tagged line; W/T, heterozygous plant.]]
@@ Line 12: / Line 12: @@
 '''Mutant'''
-The schematic diagrams of the rice CAO genes and insertion positions of T-DNA and Tos17.
+The schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17''.
-[[File:Schematic diagrams of the rice CAO genes and insertion positions of T-DNA and Tos17.jpg]]
+[[File:Schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17''.jpg|Figure 2.Schematic diagrams of the rice ''CAO'' genes and insertion positions of T-DNA and ''Tos17'']]
-* Line 1C-039-43, in which T-DNA insertion occurred in the first intron of OsCAO1; the direction of the gus reporter gene was the same.Genotyping our 30
+* Line 1C-039-43, in which T-DNA insertion occurred in the first intron of ''OsCAO1''; the direction of the gus reporter gene was the same.Genotyping our 30
 T2 progeny resulted in the isolation of 4 homozygous(Lanes 7 , 12, 18 and 27), 17 heterozygous(1, 3, 5, 6, 10, 11, 14, 15, 17, 19, 20, 21 23, 24, 25, 26, and 28, and 9), wild-type (Lanes 2, 4, 8, 9, 13, 16, 22, 29, and 30) segregating plants.
-[[File:Genotyping and GUS activity in progeny of Line 1C-039-43.jpg|Genotyping and GUS activity in progeny of Line 1C-039-43. Thirty plants were genotyped with primers a and b (upper), or primers a and RB (lower). The 0.6-kb genomicDNAbands are PCR product using primers a and b, if no T-DNA insertion; 0.4-kb bands are PCR products using primers a and RB, if T-DNA was inserted in first intron]]
+[[File:Genotyping and GUS activity in progeny of Line 1C-039-43.jpg|Figure 3.Genotyping and GUS activity in progeny of Line 1C-039-43. Thirty plants were genotyped with primers a and b (upper), or primers a and RB (lower). The 0.6-kb genomicDNAbands are PCR product using primers a and b, if no T-DNA insertion; 0.4-kb bands are PCR products using primers a and RB, if T-DNA was inserted in first intron]]
-* Line 1B-044-10, in which the Tos17 was inserted into the fourth exon of OsCAO1.In genotyping its T2 progeny (Figure 5B), we isolated three homozygous plants, all of which showed the phenotype of pale green leaves, stunted growth, and less tillering.
+* Line 1B-044-10, in which the Tos17 was inserted into the fourth exon of ''OsCAO1''.In genotyping its T2 progeny, we isolated three homozygous plants, all of which showed the phenotype of pale green leaves, stunted growth, and less tillering.
-[[File:Genotyping of T2 progeny of Line 1B-044-10.jpg|(B), ND1064 (C) and 1B-025-43 (D). LTR2 is the 3' region-specific primer of Tos17.]]
+[[File:Genotyping of T2 progeny of Line 1B-044-10.jpg|Figure 4.(B), ND1064 (C) and 1B-025-43 (D). LTR2 is the 3' region-specific primer of Tos17.]]
 ===Expression===
-OsCAO1 is induced by light and is preferentially expressed in photosynthetic tissues.OsCAO1 was expressed strongly in shoots and leaves.
+''OsCAO1'' is induced by light and is preferentially expressed in photosynthetic tissues.''OsCAO1'' was expressed strongly in shoots and leaves.
 ----
@@ Line 32: / Line 32: @@
 During the reproductive development stages, its expression level was higher in mature than in immature panicles.
-[[File:Expression profiles of OsCAO1.jpg|PCR was performed with cDNA prepared from various vegetative and reproductive organs: 1, calli; 2 and 3, shoots and roots of 7-day-old seedlings; 4, 5, and 6, leaves, stems and roots from 30-day-old plants; 7, fully expanded mature leaves at the flowering stage; 8, panicles <5 cm long; 9, panicles between 10 and 20 cm; 10, panicles between 20 and 30 cm; 11, immature seeds 2 to 5 d after pollination. Rice actin1 OsAct1 transcript was amplified as control.]]
+[[File:Expression profiles of OsCAO1.jpg|Figure 5.PCR was performed with cDNA prepared from various vegetative and reproductive organs: 1, calli; 2 and 3, shoots and roots of 7-day-old seedlings; 4, 5, and 6, leaves, stems and roots from 30-day-old plants; 7, fully expanded mature leaves at the flowering stage; 8, panicles <5 cm long; 9, panicles between 10 and 20 cm; 10, panicles between 20 and 30 cm; 11, immature seeds 2 to 5 d after pollination. Rice actin1 OsAct1 transcript was amplified as control.]]
-When seedlings were grown under continuous light, they accumulated higher levels of OsCAO1 transcript than were measured in darkgrown plants.
+When seedlings were grown under continuous light, they accumulated higher levels of ''OsCAO1'' transcript than were measured in darkgrown plants.
-[[File:Expression profiles of OsCAO1 B.jpg|Expression levels of OsCAO1 and OsCAO2 in seedlings 4, 5, and 6 d after germination; DAG grown under continuous light L or darkness D.]]
+[[File:Expression profiles of OsCAO1 B.jpg|Figure 6.Expression levels of OsCAO1 and OsCAO2 in seedlings 4, 5, and 6 d after germination; DAG grown under continuous light L or darkness D.]]
-When transferred dark-grown seedlings to light conditions,Within the first half hour after transfer, OsCAO1 transcript levels started to increase rapidly, reaching a maximum level in 2 h; when light-grown plants were transferred to dark conditions, OsCAO1 transcript levels decreased.
+When transferred dark-grown seedlings to light conditions,Within the first half hour after transfer, ''OsCAO1'' transcript levels started to increase rapidly, reaching a maximum level in 2 h; when light-grown plants were transferred to dark conditions, OsCAO1 transcript levels decreased.
-[[File:Effect of light on OsCAO1.jpg|(A) Expression levels after etiolated 4 DAG seedlings were exposed to illumination.(B) Expression patterns of 4 DAG light-grown seedlings after transfer to dark conditions.]]
+[[File:Effect of light on OsCAO1.jpg|Figure 7.(A) Expression levels after etiolated 4 DAG seedlings were exposed to illumination.(B) Expression patterns of 4 DAG light-grown seedlings after transfer to dark conditions.]]
 ----
 '''Transcript levels of ''OsCAO1'' followed a circadian rhythm'''
-Because the expression pattern of OsCAO1 was light-dependent, we examined whether its transcript level followed a circadian rhythm.Transcript levels
+Because the expression pattern of ''OsCAO1'' was light-dependent, we examined whether its transcript level followed a circadian rhythm.Transcript levels
-of OsCAO1 were higher during the daytime, but much lower in the late afternoon and at night.During the nighttime,OsCAO1 transcript levels were high.the change in expression for OsCAO1 was gradual, reaching a maximum level at 2 PM; its accumulation began in the dark.
+of ''OsCAO1'' were higher during the daytime, but much lower in the late afternoon and at night.During the nighttime,''OsCAO1'' transcript levels were high.the change in expression for ''OsCAO1'' was gradual, reaching a maximum level at 2 PM; its accumulation began in the dark.
-[[File:Circadian regulation of OsCAO1.jpg|Plants were grown in greenhouse 14 h/10 h light/dark, and then transferred to continuous darkness. Flag leaves of two or three plants were harvested for RNA extraction.]]
+[[File:Circadian regulation of OsCAO1.jpg|Figure 8.Plants were grown in greenhouse 14 h/10 h light/dark, and then transferred to continuous darkness. Flag leaves of two or three plants were harvested for RNA extraction.]]
-OsCAO1 was inducible by all light treatments, but most significantly under blue light.
+''OsCAO1'' was inducible by all light treatments, but most significantly under blue light.
-[[File:Analysis of OsCAO1 under different light conditions.jpg|Expression levels were determined in 4-day-old dark-grown wild-type seedlings after exposure to various light sources. D, darkness; Fr, far-red light; R, red light; B, blue light; W, white light.]]
+[[File:Analysis of ''OsCAO1'' under different light conditions.jpg|Figure 9.Expression levels were determined in 4-day-old dark-grown wild-type seedlings after exposure to various light sources. D, darkness; Fr, far-red light; R, red light; B, blue light; W, white light.]]
 ----
 {| class="wikitable"
@@ Line 69: / Line 69: @@
 This class of proteins (chlorophyll a oxygenase (CAO) protein) they encode contain two conserved functional motifs – the Rieske Fe–sulfur coordinating center and a non-heme mononuclear Fe-binding site.
-[[File:Phylogeneic trees of the proteins.jpg|Phylogeneic trees of the proteins containing the conserved Rieske [2Fe–2S] binding site from ''rice'' and ''Arabidopsis'']]
+[[File:Phylogeneic trees of the proteins.jpg|Figure 10.Phylogeneic trees of the proteins containing the conserved Rieske [2Fe–2S] binding site from ''rice'' and ''Arabidopsis'']]
 ==Labs working on this gene==