Os10g0577600 - Revision history

Xysj1980 at 08:37, 24 March 2017

2017-03-24T08:37:50Z

Xysj1980 at 08:37, 24 March 2017

2017-03-24T08:37:17Z

Rice2012: /* References */

2015-06-13T08:59:19Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:18:17Z

‎Structured Information

Wangzhen1991: /* Function */

2014-06-05T13:02:27Z

‎Function

Wangzhen1991 at 03:53, 5 June 2014

2014-06-05T03:53:05Z

Wangzhen1991: /* Annotated Information */

2014-06-05T03:50:35Z

‎Annotated Information

Wangzhen1991: /* Function */

2014-06-05T03:12:14Z

‎Function

Wangzhen1991: /* Function */

2014-06-05T03:11:52Z

‎Function

Wangzhen1991: /* Function */

2014-06-05T03:11:08Z

‎Function

← Older revision		Revision as of 08:37, 24 March 2017
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−	The Rice JMJ706 is a jmjC domain-containing gene, encoding H3K9 demethylase which is required for floral organ development.	+	The Rice '''''JMJ706''''' is a jmjC domain-containing gene, encoding H3K9 demethylase which is required for floral organ development.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 08:37, 24 March 2017
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−	Rice jmjC domain-containing gene ~~JMJ706 encodes~~ H3K9 demethylase required for floral organ development.	+	The Rice JMJ706 is a jmjC domain-containing gene, encoding H3K9 demethylase which is required for floral organ development.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 08:59, 13 June 2015
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	[[Category:Japonica Chromosome 10]]		[[Category:Japonica Chromosome 10]]
	[[Category:Chromosome 10]]		[[Category:Chromosome 10]]
		+	==Structured Information==

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 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 (H3K9) in vitro. Loss-of-function mutations of the gene lead to increased di- and trimethylations of H3K9 and affect the spikelet development, including altered floral morphology and organ number. Gene expression and histone modification analysis indicates that JMJ706 regulates a ubset of flower development regulatory genes.
-Histone demethylation activity has been shown to be jmjC domain dependent <ref name="ref4" /> <ref name="ref6" />. To study JMJ706 function, a truncated JMJ706 protein containing the jmjN and jmjC domains and an additional GST tag was purified from Escherichia coli cells and tested for in vitro histone demethylase activity. The incubated histones were analyzed by Western blot tests with antibodies specific to histone H3 modification modules. As shown in Fig.1, the GST-JMJ706 fusion protein reduced the level of H3K9me2 and H3K9me3 but slightly increased the level of H3K9me1. The methylation of other lysine residues was not significantly altered, except a slight decrease observed for H3K36me2. Glutathione S-transferase alone had no effect on histone modification. This suggests that JMJ706 may be mainly involved in the demethylation of H3K9me2 and H3K9me3.
+Histone demethylation activity has been shown to be jmjC domain dependent <ref name="ref4" /> <ref name="ref6" />. To study JMJ706 function, a truncated JMJ706 protein containing the jmjN and jmjC domains and an additional GST tag was purified from ''Escherichia coli'' cells and tested for in vitro histone demethylase activity. The incubated histones were analyzed by Western blot tests with antibodies specific to histone H3 modification modules. As shown in Fig.1, the GST-JMJ706 fusion protein reduced the level of H3K9me2 and H3K9me3 but slightly increased the level of H3K9me1. The methylation of other lysine residues was not significantly altered, except a slight decrease observed for H3K36me2. Glutathione S-transferase alone had no effect on histone modification. This suggests that JMJ706 may be mainly involved in the demethylation of H3K9me2 and H3K9me3.
 ===Evolution===

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-Please input one-sentence summary here.
+Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development.
 ==Annotated Information==
-===Bacground===
+===Background===
 Histone lysine methylation is an important epigenetic modification with both activating and repressive roles in gene expression <ref name="ref1" /> . There are six lysine residues in histone N-termini that are predominantly methylated, with the methylation of histone H3 lysine 4 (H3K4) and lysine 36 (H3K36) primarily having an activating function, whereas the methylation of histone H3 lysine 9 (H3K9) and lysine 27 (H3K27) and histone H4lysine 20 (H4K20) is essentially associated with repressed chromatin <ref name="ref2" />. Histone lysine residues can be mono-, di- or trimethylated. Each distinct methyl state is associated with different biological functions <ref name="ref1" />.

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 T-DNA insertion mutant lines . The FLAG-tagged protein was also observed in enriched DAPI-stained domains (Fig. 3C).
-===Mutant===
+===Mutation===
 [[File:Mutant.jpg|left|thumb|200px|"Fig.4 JMJ706 loss-of-function mutation affects spikelet development.(from reference <ref name="ref5"/>)."]]

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 ===Function===
-[[File:Function_甲基化.jpg|right|thumb|200px|"Fig.1 In vitro assays of JMJ706 histone demethylation activity (from reference <ref name="ref5"/>)."]]
+[[File:Function_甲基化.jpg|right|thumb|150px|"Fig.1 In vitro assays of JMJ706 histone demethylation activity (from reference <ref name="ref5"/>)."]]
 JMJ706 encodes a heterochromatin-associated H3K9 demethylase involved in the regulation of flower development in rice. Histone lysine ethylation is an important epigenetic modification with both activating and repressive roles in gene expression. Jumonji C (jmjC) domain-containing proteins have been shown to reverse histone methylation in non plant model systems. Here, we show that plant Jumonji C proteins have both conserved and specific features compared with mammalian homologues. In particular, the rice JMJD2 family jmjC gene JMJ706 is shown to encode a heterochromatin-enriched protein. The JMJ706 protein specifically reverses di- and trimethylations of lysine 9 of histone H3