Os12g0438600 - Revision history

Rice2012: /* References */

2015-06-13T09:17:22Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:33:02Z

‎Structured Information

Yangqingsong: /* Function */

2014-06-07T08:52:05Z

‎Function

Yangqingsong: /* Evolution */

2014-06-07T08:49:57Z

‎Evolution

Yangqingsong: /* Expression */

2014-06-07T08:48:52Z

‎Expression

Yangqingsong: /* Expression */

2014-06-07T08:46:26Z

‎Expression

Yangqingsong: /* Expression */

2014-06-07T08:44:22Z

‎Expression

Yangqingsong: /* Expression */

2014-06-07T08:41:54Z

‎Expression

Yangqingsong: /* Expression */

2014-06-07T08:40:45Z

‎Expression

Yangqingsong: /* Evolution */

2014-06-07T08:36:55Z

‎Evolution

← Older revision		Revision as of 09:17, 13 June 2015
Line 59:		Line 59:
	[[Category:Japonica Chromosome 12]]		[[Category:Japonica Chromosome 12]]
	[[Category:Chromosome 12]]		[[Category:Chromosome 12]]
		+	==Structured Information==

@@ Line 53: / Line 53: @@
 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 3: / Line 3: @@
 == Function ==
-[[File:Representation of the AtCLCaNO32Ht antiporter in a plant cell.JPG|right|200px|thumb|Representation of the AtCLCaNO32Ht antiporter in a plant cell.]]
+[[File:Representation of the AtCLCaNO32Ht antiporter in a plant cell.JPG|right|250px|thumb|Representation of the AtCLCaNO32Ht antiporter in a plant cell.]]
-[[File:Vacuolar chloride is transported differently.JPG|right|200px|thumb|Vacuolar chloride is transported differently by native AtCLCaWT andthe mutant AtCLCaP160S.]]
+[[File:Vacuolar chloride is transported differently.JPG|right|250px|thumb|Vacuolar chloride is transported differently by native AtCLCaWT andthe mutant AtCLCaP160S.]]
-[[File:Homology model of the AtCLCa C-terminal region.jpg|right|200px|thumb|Homology model of the AtCLCa C-terminal region. a, the alignment used for the homology modeling of AtCLCa C-terminal region on the hCLC-5 C-terminal region. b, detail of the region putatively involved in the ATP-AtCLCa interaction.]]
+[[File:Homology model of the AtCLCa C-terminal region.jpg|right|250px|thumb|Homology model of the AtCLCa C-terminal region. a, the alignment used for the homology modeling of AtCLCa C-terminal region on the hCLC-5 C-terminal region. b, detail of the region putatively involved in the ATP-AtCLCa interaction.]]
 AtCLC-a, a member of the CLC family of anion transporters, is involved mainly in nitrate storage in the vacuole. CLCa (AtCLCa) is localized to an intracellular membrane, the tonoplast of the plant vacuole, with anion transport ability. AtCLCa is able to accumulate specifically nitrate in the vacuole and behaves as a NO32-/H+ exchanger.
@@ Line 16: / Line 16: @@
 ATP reversibly inhibits AtCLCa by interacting with the C-terminal domain. Applying the patch clamp technique to isolated Arabidopsis thaliana vacuoles, Alexis De Angeli demonstrate that ATP reduces AtCLCa activity with a maximum inhibition of 60%. ATP inhibition of nitrate influx into the vacuole at cytosolic physiological nitrate concentrations suggests that ATP modulation is physiologically relevant. ADP and AMP do not decrease the AtCLCa transport activity; nonetheless, AMP (but not ADP)  competes with ATP, preventing inhibition<ref name="ref6" />.
 == Expression ==

@@ Line 32: / Line 32: @@
 == Evolution ==
-[[File:08.jpg|200px|thumb|left|Phylogeny of predicted AtCLC proteins.]]
+[[File:08.jpg|200px|thumb|right|Phylogeny of predicted AtCLC proteins.]]
 The AtCLC proteins were predicted to consist of 9–11 transmembrane spanning domains and two conserved CBS domains at the carboxyl end. These domains were similar in sizes and positions to those of CLC-0 and mammalian CLCs.<ref name="ref2" />.
 Phylogeny of predicted AtCLC proteins shown in fiugre.

@@ Line 25: / Line 25: @@
-<gallery widths=230px heights=490px perrow=3 caption="The expression of AtCLC-a (1)AtCLC-Promoter::GUS activity;(2)Organ and tissue-specific expression of AtCLC members; (3)Transient expression of AtCLCa–GFP fusion proteins in protoplasts.">
+<gallery widths=250px heights=530px perrow=3 caption="The expression of AtCLC-a (1)AtCLC-Promoter::GUS activity;(2)Organ and tissue-specific expression of AtCLC members; (3)Transient expression of AtCLCa–GFP fusion proteins in protoplasts.">
 File:Organ and tissue-specific expression of AtCLC members.jpg
 File:Histochemical staining of AtCLC-Promoter.jpg

@@ Line 24: / Line 24: @@
 To determine the subcellular localization of the AtCLCa protein,  green fluorescent protein (GFP) fusion proteins were transiently expressed in protoplasts from Arabidopsis cell suspensions. Confocal microscopy studies revealed a green fluorescent labelling that coincided with the tonoplast<ref name="ref1" />.
-[[File:Transient expression of AtCLCa–GFP fusion proteins in protoplasts.JPG|right|300px|thumb|Representation of the AtCLCaNO32Ht antiporter in a plant cell.]]
-<gallery widths=200px heights=400px perrow=2 caption="sunflowers are groovy">
+<gallery widths=200px heights=400px perrow=2 caption="(1)AtCLC-Promoter::GUS activity;(2)Organ and tissue-specific expression of AtCLC members; (3)Transient expression of AtCLCa–GFP fusion proteins in protoplasts.">
 File:Organ and tissue-specific expression of AtCLC members.jpg
 File:Histochemical staining of AtCLC-Promoter.jpg
 </gallery>