Os12g0472500 - Revision history

Rice2012: /* References */

2015-06-13T09:17:29Z

‎References

Lmw2015: /* Headline text */

2015-06-12T04:33:21Z

‎Headline text

Suchao13: /* References */

2014-06-02T05:33:13Z

‎References

Suchao13: /* Lab */

2014-06-02T05:30:53Z

‎Lab

Suchao13: /* Annotated Information */

2014-06-02T05:29:12Z

‎Annotated Information

Suchao13: /* Evolution */

2014-06-02T05:28:32Z

‎Evolution

Suchao13: /* Evolution */

2014-06-02T05:27:51Z

‎Evolution

Suchao13: /* Expresson */

2014-06-02T05:24:48Z

‎Expresson

Suchao13: /* MIL2 sequence analysis */

2014-06-02T05:21:53Z

‎MIL2 sequence analysis

Suchao13: /* Annotated Information */

2014-06-02T05:18:40Z

‎Annotated Information

← Older revision		Revision as of 09:17, 13 June 2015
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	[[Category:Japonica Chromosome 12]]		[[Category:Japonica Chromosome 12]]
	[[Category:Chromosome 12]]		[[Category:Chromosome 12]]
		+	==Structured Information==

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 * Lilan Hong, Ding Tang, Yi Shen, ''et al.'' MIL2 (MICROSPORELESS2) regulates early cell differentiation in the rice anther. New Phytologist (2012) 196: 402–413
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 05:33, 2 June 2014
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	===References===		===References===
		+	* Xinai Zhao, Justina de Palma, Rowena Oane, ''et al.'' OsTDL1A binds to the LRR domain of rice receptor kinase MSP1, and is required to limit sporocyte numbers. The Plant Journal (2008) 54, 375–387
		+	* Lilan Hong, Ding Tang, Yi Shen, ''et al.'' MIL2 (MICROSPORELESS2) regulates early cell differentiation in the rice anther. New Phytologist (2012) 196: 402–413

	== Headline text ==		== Headline text ==

← Older revision		Revision as of 05:30, 2 June 2014
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	===Lab===		===Lab===
		+	* State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;
		+
		+	* Biotechnology Research Institute/National Key Facility for Gene Resources and Gene Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
		+
		+	* College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
		+
		+	* Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, Manila, Philippines
		+
		+	* Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, Canberra, Australia

	===References===		===References===

← Older revision		Revision as of 05:29, 2 June 2014
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	[[File:1A.jpg]]		[[File:1A.jpg]]
		+
		+	===Lab===
		+
		+	===References===

	== Headline text ==		== Headline text ==

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 OsTDL1A and OsTDL1B are rice homologs of TPD1 of Arabidopsis
-The full-length protein sequence of Arabidopsis TPD1 [AAR25553, 176 amino acids (aa)] was used as the query in a tblastn search of the rice genome. We detected two TPD1-like genes and named them OsTDL1A (blaste-value: 5 × 10−27) and OsTDL1B (blaste-value: 1 × 10−18). They are located on chromosomes 12 and 10, respectively, and the corresponding full-length cDNAs are AK108523 and AK121594. When their predicted protein sequences (NP_001066753, 226 aa; NP_001064316, 169 aa) were used as tblastn queries of the Arabidopsis genome, the best hits were to TPD1 and another Arabidopsis protein (ABF59206, 179 aa), which we have named AtTDL1. TPD1 is more similar to AtTDL1 (e-value: 2 × 10−35) than OsTDL1A is to OsTDL1B (e-value: 2 × 10−14), a conclusion supported by CLUSTALW analysis (Figure 1A). The four proteins cluster separately from the next most similar proteins encoded by the rice and Arabidopsis genomes (the boxed proteins are from rice).
+The full-length protein sequence of Arabidopsis TPD1 [AAR25553, 176 amino acids (aa)] was used as the query in a tblastn search of the rice genome. We detected two TPD1-like genes and named them OsTDL1A (blaste-value: 5 × 10−27) and OsTDL1B (blaste-value: 1 × 10−18). They are located on chromosomes 12 and 10, respectively, and the corresponding full-length cDNAs are AK108523 and AK121594. When their predicted protein sequences (NP_001066753, 226 aa; NP_001064316, 169 aa) were used as tblastn queries of the Arabidopsis genome, the best hits were to TPD1 and another Arabidopsis protein (ABF59206, 179 aa), which we have named AtTDL1. TPD1 is more similar to AtTDL1 (e-value: 2 × 10−35) than OsTDL1A is to OsTDL1B (e-value: 2 × 10−14), a conclusion supported by CLUSTALW analysis (Figure 1A). The four proteins cluster separately from the next most similar proteins encoded by the rice and Arabidopsis genomes (the boxed proteins are from rice). Single amplicons of the expected size for OsTDL1A and OsTDL1B (546 and 485 bp, respectively) were generated from RNA of roots and spikelets (1 mm, 3 mm and 5 DAF). Three amplicon sizes were seen for MSP1, as was also reported by Nonomura et al. (2003). The sizes of these amplicons are consistent with the sizes expected for the fully spliced transcript (507 bp), a transcript in which only the first intron has been removed by splicing (752 bp) and an unspliced transcript (1298 bp), based on the full-length cDNA sequence (AK120933). The smallest amplicon was amplified most strongly from 1- and 3-mm spikelets. The largest amplicon was not a PCR product derived from possible DNA contamination, because the RNA preparations lacked DNA contamination as judged by the failure of the GAPDH primers to produce the genomic amplicon (Figure 2, open arrow). We conclude that MSP1, OsTDL1A and OsTDL1B are all expressed in spikelets before and during meiosis.
 [[File:1A.jpg]]

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 ===Expresson===
-qRT-PCR shows MIL2 was expressed at a very low level, it was observed in most of the tissues analyzed, including leaf, internode, leaf sheath and panicle at early developmental stages. MIL2 expression in panicles declined gradually as the panicles elongated, and hardly any transcripts could be detected in panicles that were 10 cm in length.
+qRT-PCR shows MIL2 was expressed at a very low level, it was observed in most of the tissues analyzed, including leaf, internode, leaf sheath and panicle at early developmental stages. MIL2 expression in panicles declined gradually as the panicles elongated, and hardly any transcripts could be detected in panicles that were 10 cm in length.(R, root; I,internode; L, leaf; Sh, sheath; S, seedling; P1, 1-cm panicles; P2, 3-cm panicles; P3, 6-cm panicles; P4, 10-cm panicles; P5, panicles near maturity.)
 [[File:mil2-3.jpg]]
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 RNA in situ hybridization shows MIL2 was expressed conspicuously in archesporial cells located in the four corners of young anthers. As archesporial cells developed into sporogenous cells and parietal cells, MIL2 mRNA preferentially accumulated in parietal cells, first in PPCs, and then in inner SPCs; hybridization signals in sporogenous cells were considerably weaker. After differentiation of the four somatic cell layers, MIL2 was expressed mainly in the somatic cell layers embracing the reproductive cells, including the middle and tapetal layers. A low level of expression was observed in microsporocytes in the
 center of each anther lobe, whereas, in the outer two layers, hardly any hybridization signal above background was detectable. Expression of MIL2 in the anther lobes decreased substantially as the microsporocytes underwent meiosis.
 === '''Evolution''' ===

← Older revision		Revision as of 05:21, 2 June 2014
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	===MIL2 sequence analysis===		===MIL2 sequence analysis===
		+	The predicted MIL2 protein has 226 amino acids, including a 34- amino-acid signal peptide at the N terminus.A BLAST search of the database indicated that MIL2 is well conserved in land plants and generally has two homologs in many angiosperm species, including maize (Zea mays), rice, soybean Glycine max) and grape (Vitis vinifera). The closest homolog of MIL2 is the maize ''MULTIPLE ARCHESPORIAL CELLS 1 (MAC1'') gene. However, no clear MIL2 homologs could be
		+	detected in the Chlorophyta. The predicted MIL2 homolog proteins all have a highly conserved region (corresponding to positions 112–220 in AK713941), which we termed the MIL2 homology domain. These proteins are typically c. 160–230 amino acids in length and carry the MIL2 homology region at the C terminus. In addition, based on Signal IP predictions, most of the homologs have N-terminal signal peptides similar to MIL2.
		+
		+	[[File:mil2-0.jpg]]

	=== Mutation and Phenotype===		=== Mutation and Phenotype===

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 * MIL2 works upstream of UDT1(is an early regulator of rice tapetum development) in controlling anther wall cell development.
 === Mutation and Phenotype===
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 RNA in situ hybridization shows MIL2 was expressed conspicuously in archesporial cells located in the four corners of young anthers. As archesporial cells developed into sporogenous cells and parietal cells, MIL2 mRNA preferentially accumulated in parietal cells, first in PPCs, and then in inner SPCs; hybridization signals in sporogenous cells were considerably weaker. After differentiation of the four somatic cell layers, MIL2 was expressed mainly in the somatic cell layers embracing the reproductive cells, including the middle and tapetal layers. A low level of expression was observed in microsporocytes in the
 center of each anther lobe, whereas, in the outer two layers, hardly any hybridization signal above background was detectable. Expression of MIL2 in the anther lobes decreased substantially as the microsporocytes underwent meiosis.
 === '''Evolution''' ===