Os12g0569700 - Revision history

Rice2012: /* References */

2015-06-13T09:18:10Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:34:35Z

‎Structured Information

Zhangye: /* Expression */

2015-01-02T16:58:56Z

‎Expression

Zhangye at 16:57, 2 January 2015

2015-01-02T16:57:32Z

127.0.0.1: Created page with "{{JaponicaGene| GeneName = Os12g0569700| Description = Similar to Non-cell-autonomous heat shock cognate protein 70| Version = NM_001190087.1 GI:297729304 GeneID:9268687| ..."

2012-06-21T10:34:29Z

Created page with "{{JaponicaGene| GeneName = Os12g0569700| Description = Similar to Non-cell-autonomous heat shock cognate protein 70| Version = NM_001190087.1 GI:297729304 GeneID:9268687| ..."

New page

{{JaponicaGene|
GeneName = Os12g0569700|
Description = Similar to Non-cell-autonomous heat shock cognate protein 70|
Version = NM_001190087.1 GI:297729304 GeneID:9268687|
Length = 5024 bp|
Definition = Oryza sativa Japonica Group Os12g0569700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:23645878..23650901|
CDS = 23649325..23649794|
GCID = <gbrowseImage1>
name=NC_008405:23645878..23650901
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:23645878..23650901
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>gatgccaagcgtctcatcggccagagatttactgatgcttctgtccagagtgatattatgctctggcccttcaaggtcatcgctggacctggtgacaagtcaatgattgtggtccagtacaagggtgaagagaagcagtttgcagctgaggagatctcttctatggtcctcatcaagatgcgtgagattgctgaagcttaccttggcaccaccataaaaaaagctgtcgtaacagttcctgcttacttcaactactcacagaggcaggctaccaaggacgctagggtcattgctggcctcaatgtcatgcgtatcatcaatgagcctactgctgctgccattgcctatggtcttgacaagaaggcttccagtgttggtgagaagaacgtcctcatcttcgaccttggtggtggtacctttgatgtctctcttctcaccattgaggagggaggtcaaggccacagctggtga</cdnaseq>|
AA = <aaseq>DAKRLIGQRFTDASVQSDIMLWPFKVIAGPGDKSMIVVQYKGEE KQFAAEEISSMVLIKMREIAEAYLGTTIKKAVVTVPAYFNYSQRQATKDARVIAGLNV MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGGQGHSW</aaseq>|
DNA = <dnaseqindica>3448..3917#actcaagtcctcccatccagcagctcagccgccacaagccatggccgatgcgagctcggccgccgtcgctgcccccaagccccaactggccgccggcacttgagcaccaagcagggccagctcgacgacgccgtcgccgcccgccaccaagctcgaacagatcagctccagcaatgttcaatccaacgacttttgcattgattgattgattttctctgctcgtgtgattgtgtgattggaatcgactagcttgaaccaattgatagtatccctgtgctgtgcgtgcattgcatgtgtacatattaccaaatctctgttgcatatggatccactcttggatatcggttttcgcttgaatgccgttttcttctgttattttgttgttcctttttttaagctataaaacccaaccttctctttatgatctgtttgttttcaatattgtaattttttttctggaatttgttgttaattataaccttttcattatctcctgagctagcagattgatgatgaaacaatttctccaagcgtttgttacgctgacatccatatatgatggagggctatgttaccatagccttcatagttttcaatctgaaccatctgctgctcaatcattttccccagattttttttacagcgattgtttctccattgctaactgttctgtattgatttgggattcaaattcctttcatggtttttgctcgttttctttacaatctcattcatttgtctgtgcaacatttgggaaaaattgggagaagtgatgatattatatccataccacatttctgcattcactatgatgatcattaacttcatgcactaccatctgatctcccactacacataataaagttatggtacaaggtatataaattgaagttgtgcaatgatggactgattttcatttacacgctgaatttattttgttaattaatgtgcaatctgtgatgcaaataatcccatttgctgaatcactgtgattattgcaatcaaaatcaccatctttcggctgagattgcacattgttattctgcaaaatttatctttgttttaaattaaggtttatgccatttggtatctgacacataatcatttctctgattatgtgtctcgaaacatggcagaaagtgagggggaacggtccttgcgacactggttgctactatatgagcttctagttactatatacccgtttacatttccttgcttttttagtatttctgtttaacttttgtcatgcctatgtgttgtgatgcattagttttgtactgtggagattgtacccctgtcaaaccacttaatgaaattacagataattattagactgaaaatgccgtcttttggctgaatcactctgatcaatcaaaatcaccatatttcagctgagattgcacattgttgttacgcgaaacaattttatctttgttttaaattaaggttcagccatttggtatctgacacataatcatttctctgattacatgtctcgaaacatggcagaaagtaagggggaacggtccttgcgatgctggttgctgctatatgagcttctagttactatatacccgtttacatatcctttgctttcttagtatttctgtttggctttattcattagttatgcctatgtgttgtaatgcattagttttgtactgtggagattgtatgtctatcaaactacttaatgaagttacagataaatattagactgaaagtctaaaatcactgtagcatattgggctaaaaggtatagtttgtttacttggaatctctcgaaagtttgtttgattgctgaatttgtgctccagtttgtttgctgtttcttacatgaagcattacctgtcctgatgttatttatcacttatgagatttgtgtatgcagtttccattttttcagtgcctgttatgcagatgccaagcgtctcattggccggagatttactgatgcttctgtccagagtgatattaagctctggcccttcaaggtcattgctggacctggtgacaagtcaacgattgtggttcagtacaagggtgaagagaagcaggttgcagctgaggtaatattcaaattgtttctgtgatggctacttaggatttatgctatgattttaacttgcattgttgtaatgatgttgttaaagtaaacaagtaacggtagcaagtttgcttatcagcttgttgtttttcagtttttcctgtgagcctatcttttcgttcttttccattataaaaacccatctctctttatggtctgtttgttttctatgttataaatgtttttctgtaatttgttgaccttttcattaaatcttgagctagcagattgatgatgaaacaatttctccaagcgtttgttacgctgatccatatgatggagggctatggtaacatagccttcatagttttcaatctgaaccatctgctggtcaatcattttccccagatttttttacagcgattgtttctccatgcatacagttctgtattgatttgggattgtactaacacttagcataacatacattaatgaattcctttcaaattaagatttctattatagtgctattcaaattccttatatgatttttgctcgttttctttacaaaatctcattcaattttctgtgcaacgtttgggaaaaaagtattatttgggagaagtgatgatattatatccataccacatttctgcatttgctatgatgatcattaaattcatgcactaccatctgatctcccactacacatgataaagttattgttacaaggctatataaattgaagttatgcaatgctggactgattttcatttacaagctgaatttattttgttagtgtgcaatctgtgatgcaaataatcccatttgctgaatcactgtgattattgcaataaaaatcaccatcttttggctgagattgcacattgttctgcaaaacatttttatatttgttttaaattaaggttcatgccatttggtatctgacacataatcatttatctgattatgcgtctcgaaacatggcagaaagcgagggggaacggtccttgcgacactggttgctgctatatgagcttctagttactatatacccgtttacatatcccttgctttcttagtatttctgtttggctttattcattagttatgcctatgtgttgtgatacatagttttgtactgtggagattgtacgtctgtcaaactacttaatgaaattacagattattggaccgaaagtctgaaactactatagtatattagactaaggttatatagtttgtttacttggaattgctccaaagtttgtctgattgctgaatttgtactccgtttgtttgctgtttttcgcatgaaacattaccagtcctgaagatatttatcatacatgaggtttgtgtatgcagtttctgattttttagtgcctcttatgcagatgccaagcgtctcatcggccagagatttactgatgcttctgtccagagtgatattatgctctggcccttcaaggtcatcgctggacctggtgacaagtcaatgattgtggtccagtacaagggtgaagagaagcagtttgcagctgaggagatctcttctatggtcctcatcaagatgcgtgagattgctgaagcttaccttggcaccaccataaaaaaagctgtcgtaacagttcctgcttacttcaactactcacagaggcaggctaccaaggacgctagggtcattgctggcctcaatgtcatgcgtatcatcaatgagcctactgctgctgccattgcctatggtcttgacaagaaggcttccagtgttggtgagaagaacgtcctcatcttcgaccttggtggtggtacctttgatgtctctcttctcaccattgaggagggaggtcaaggccacagctggtgacacgcatcttggtggtgaggactttgacaacagaatggtgaagcactttgttcaggagttcaagaggaagagcaagaaggacatcactggcaacccaaggcctgtcgggaggctgaggacagcttgtgagtgggcgaagaggactctctcccctccacttcccagaccacccaccattgagattgactccctgtacgagggcatcgacttctactcgaacatcacctgcgccaggttcgaggagctgaccatggatctcttcaggaagtgcatgaggggataccaagatggacaaagagcagcgtgcacgatgttcatccttgttggtggctccaccaggattcccagagtgcagcagctcctccaggacttcttcaatggcaaggagctctgcaagaacatcaaccctgatgaggccgtcgcctatggcgccgctgtccaggcccccatcttggtctggagacggccagaggagtgatgacctagaaggagcaggccggtcttctccacctactccgacaaccggcctggtgtgttgatccaggtgtacgagggcgatgggagaggaccatgacaccattgagtaagtgaggagatcgagaagatggtccaggacgcggagaagtacaagtcggaggacgatgagctacatcgaagaacatgcacaaacaccatcaaggtccgagcacccacgcggcggccaagaagatgggaggacgcggtgggagattgagccaagatggacgaggacgtgccgttaaattttggctgatcatggtggttaaattttggctacgttgtaatgaaaaatgcattggttgcttcgttaaagtaatagtactacaattgttctgtttcggttcatccgcatttgccttttgatttttgtttagctgctacttaaatcgcgtttattttgcatacagtatctatctgtcttctccaaagcgttggggtgtgcttttcttccccggtggtattcactattcagagtcttttgtcgtcgtgtctgctctaatatgccatccattgtagggagtggattacgtccccccatcgtgtatctttttctttcaaaatcaatgcaaatagttgttaaaaattctg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001190087.1 RefSeq:Os12g0569700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

← Older revision		Revision as of 09:18, 13 June 2015
Line 85:		Line 85:
	[[Category:Japonica Chromosome 12]]		[[Category:Japonica Chromosome 12]]
	[[Category:Chromosome 12]]		[[Category:Chromosome 12]]
		+
		+	==Structured Information==

@@ Line 25: / Line 25: @@
 {| class='wikitable' style="text-align:center"
 |-
-! | Primer(<ref name="ref1"/>)
+! | Primer<ref name="ref1"/>
 ! | Forward primer
 ! | Reverse primer

← Older revision		Revision as of 16:57, 2 January 2015
Line 1:		Line 1:
		+	''OsHsp23.7'' is a member of Heat shock proteins(Hsps), it belongs to HSP70 family<ref name="ref1"/><ref name="ref2"/>.
		+	==Annotated Information==
		+	===Function===
		+	[[File: OsHsp23.7 expression1.jpg\|left\|thumb\|200px\|'''Figure 1.'''''Drought tolerance assays of OsHsp23.7-OE transgenic rice(from reference <ref name="ref1"/>).'']]
		+	[[File: OsHsp23.7 expression2.jpg\|right\|thumb\|200px\|'''Figure 2.'''''Salt tolerance assays of OsHsp23.7-OE transgenic rice(from reference <ref name="ref1"/>).'']]
		+	Both OsHsp17.0-OE and OsHsp23.7-OE transgenic lines demonstrated higher germination ability compared to wild-type (WT) plants when subjected to mannitol and NaCl. Phenotypic analysis showed that transgenic rice lines displayed a higher tolerance to drought and salt
		+	stress compared to WT plants. In addition, transgenic rice lines showed significantly lower REC, lower MDA content and higher free proline content than WT under drought and salt stresses. These results suggest that ''OsHsp17.0'' and ''OsHsp23.7'' play an important role in rice acclimation to salt and drought stresses and are useful for engineering drought and salt tolerance rice<ref name="ref1"/>.
		+
		+	'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0005524 GO:0005524]
		+
		+	===Mutation===
		+	[[File: nine OsHSP genes.jpg\|left\|thumb\|300px\|'''Figure 3.'''''Gene tree of the nine rice HSPs(from reference <ref name="ref2"/>).'']]
		+	*The expression of ''OsHsp17.0'' and ''OsHsp23.7'' in transgenic lines (T2 generation) was checked by real-time qPCR. Three highly
		+	over-expressed lines were identified in the OsHsp17.0-OE and OsHsp23.7-OE lines respectively (Fig. 1A) and were used for later
		+	characterization.
		+	*Three-week-old seedlings were exposed to air for 9.5 h, and then were transferred to normal hydroponic conditions and recovered for 10 d.(Figure 1D) Seedlings before treatment. (Figure 1E) Seedlings were exposed to air for 9.5 h. (Figure 1F) Seedlings were transferred to normal hydroponic conditions and recovered for 10 d.
		+	*Three-week-old seedlings were watered with 200 mM NaCl solution for 24 h and then were transferred to normal hydroponic conditions and recovered for 10 d. (Figure 2D) Seedlings before treatment. (Figure 2E) Seedlings were subjected to 200 mM NaCl solution for
		+	24 h. (Figure 2F) Seedlings were transferred to normal hydroponic conditions and recovered for 10 d.
		+
		+	===Expression===
		+	*Overexpression of ''OsHsp17.0'' and ''OsHsp23.7'' enhanced drought tolerance and salt tolerance.
		+	*After temperature treatment, leaf tips of both transgenic plants and WT plants were rolled or withered, no significant differences were observed between them. After recovery for 7 d, both transgenic plants and WT plants remained alive and produced new leaves.
		+	*There were no significant differences in content of MDA content and proline content between WT and OE lines under normal conditions. However, after stress treatments (20%PEG6000, 200 mM NaCl, 45℃ and 5℃), the MDA content was significantly higher in WT than in OE lines. The accumulation of proline in OE plants was significantly more than that in WT plants, which suggesting that less membrane damage and improved osmotic adjustment ability in transgenic OE plants under stresses.
		+
		+	{\| class='wikitable' style="text-align:center"
		+	\|-
		+	! \| Primer(<ref name="ref1"/>)
		+	! \| Forward primer
		+	! \| Reverse primer
		+	\|-
		+	\| rowspan="2"\|PCR-amplifie
		+	\| \| 5'-CGGGATCCCACCCAACTCTTCTTCTTCC-3'
		+	\| \| 5'-GCTCTAGACTTGACCTTGACAAACTCCC-3'
		+	\|-
		+	\| \| 5'-CGGGATCCATGAGCCTACTGCTGCT-3'
		+	\| \| 5'-AATCTAGATCGTACACCTGGATCAACA-3'
		+	\|-
		+	\| rowspan="3"\|real-time quantitative PCR
		+	\| \| 5'-AAGGTGGACCAGGTGAAGG-3'
		+	\| \| 5'-GACCTTGACAAACTCCCGTT-3'
		+	\|-
		+	\| \| 5'-AGACCACCCACCATTGAGATT-3'
		+	\| \| 5'-GCCACCAACAAGGATGAACAT-3'
		+	\|-
		+	\| \| 5'-GGAAGTAAGGAAGGAGGAGGAA-3'
		+	\| \| 5'-CAGAGGTGATGCTAAGGTGTTC-3'
		+	\|}
		+
		+	===Evolution===
		+	Gene tree were conducted using the software Molecular Evolutionary Genetics Analysis (MEGA) Version 4.0 by the neighbor-joining method with pairwise deletion and the Poisson correction model.As shown in Figure 1, based on amino acids sequence homology, nine OsHSP genes were divided into three classes, among them ''OsHSP80.2'', ''OsHSP74.8'' and ''OsHSP50.2'' belong to ''HSP90'' family; ''OsHSP71.1'', ''OsHSP58.7'' and ''OsHSP23.7'' belong to ''HSP70'' family; ''OsHSP26.7'', ''OsHSP24.1'' and ''OsHSP17.0'' belong to sHSP family<ref name="ref2"/>.
		+
		+	===Knowledge Extension===
		+	Heat shock proteins (Hsps) belong to a class of proteins that are conserved in prokaryotes and eukaryotes and are especially abundant in plants. Hsps are highly expressed in plants and other organisms after being stimulated by high temperature and other stresses. The sHsps are much more abundant in higher plants than in other organisms<ref name="ref3"/>.
		+	Expression of nine ''OsHSP'' genes was affected differentially by abiotic stresses and abscisic acid (ABA). All nine OsHSP genes were induced strongly by heat shock treatment, whereas none of them were induced by cold. The transcripts of ''OsHSP80.2'', ''OsHSP71.1'' and
		+	''OsHSP23.7'' were increased during salt tress treatment. Expression of ''OsHSP80.2'' and ''OsHSP24.1'' genes were enhanced while treated with 10% PEG. Only ''OsHSP71.1'' was induced by ABA while OsHSP24.1 was suppressed by ABA. These observations imply
		+	that the nine ''OsHSP'' genes may play different roles in plant development and abiotic stress responses<ref name="ref2"/>.
		+	According to their approximate molecular weights, HSPs are grouped into five families: HSP100s, HSP90s, HSP70s, HSP60s and sHSPs (small
		+	HSPs, o40 kDa)<ref name="ref4"/>. Most HSPs function as molecular chaperones in maintaining homeostasis of protein folding and are thought to be responsible for the acquisition of thermo tolerance<ref name="ref3"/>. It is believed that the accumulations of HSPs play a pivotal role in abiotic stress.
		+
		+	==Labs working on this gene==
		+	*Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, China
		+	*College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
		+	*Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China
		+
		+	==References==
		+	<references>
		+	* <ref name="ref1">
		+	Zou J, Liu C, Liu A, et al. Overexpression of< i> OsHsp17. 0</i> and< i> OsHsp23. 7</i> enhances drought and salt tolerance in rice[J]. Journal of plant physiology, 2012, 169(6): 628-635.
		+	</ref>
		+	* <ref name="ref2">
		+	Zou J, Liu A, Chen X, et al. Expression analysis of nine rice heat shock protein genes under abiotic stresses and ABA treatment[J]. Journal of plant physiology, 2009, 166(8): 851-861.
		+	</ref>
		+	* <ref name="ref3">
		+	Vierling E. The roles of heat shock proteins in plants[J]. Annual review of plant biology, 1991, 42(1): 579-620.
		+	</ref>
		+	* <ref name="ref4">
		+	Trent J D. A review of acquired thermotolerance, heat‐shock proteins, and molecular chaperones in archaea[J]. FEMS microbiology reviews, 1996, 18(2‐3): 249-258.
		+	</ref>
		+	</references>
		+
		+	==Structured Information==
	{{JaponicaGene\|		{{JaponicaGene\|
	GeneName = Os12g0569700\|		GeneName = Os12g0569700\|

@@ Line 79: / Line 79: @@
 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]