Os12g0597000 - Revision history

Xysj2019 at 02:02, 7 March 2017

2017-03-07T02:02:08Z

Xysj2019 at 02:01, 7 March 2017

2017-03-07T02:01:42Z

Xysj2019 at 02:01, 7 March 2017

2017-03-07T02:01:12Z

Rice2012: /* References */

2015-06-13T09:18:35Z

‎References

Lmw2015: /* Structured Information */

2015-06-12T04:35:35Z

‎Structured Information

Shuimuxixia at 05:52, 2 June 2014

2014-06-02T05:52:13Z

Shuimuxixia at 05:50, 2 June 2014

2014-06-02T05:50:53Z

Shuimuxixia at 05:50, 2 June 2014

2014-06-02T05:50:21Z

Shuimuxixia at 05:49, 2 June 2014

2014-06-02T05:49:05Z

Shuimuxixia at 05:47, 2 June 2014

2014-06-02T05:47:57Z

← Older revision		Revision as of 02:02, 7 March 2017
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	===Expression===		===Expression===
−	[[File:图片4-5.png\|left\|thumb\|200px\|'''Figure 5''' OsCBL2 but not OsCBL1shows GA-specific up-regulation in embryoless rice half-grains. Total RNA was isolated from grains treated with ABA or GA (A) or no hormone (B) for the indicated times. Note that changes in mRNA abundance reflect changes occurring in the aleurone layer.(from reference<ref name="ref1" />)]]
−	[[File:图片1-6.png\|right\|thumb\|200px\|'''Figure 6''' The rice calcineurin B-like gene OsCBL2 is up-regulated by GA treatment of rice aleurone layers. Transcript abundance of OsCBL2 (black circles) and actin (white circles) as measured by hybridization to a rice oligonucleotide chip (A). Total RNA was extracted from embryoless rice half-grains treated with GA, ABA, or no hormone for the indicated time. Expression of GA-induced α-amylase, RAmy1A (B), and ABA-induced dehydrin (C) genes in the same chip experiment are shown for comparison.(from reference<ref name="ref1" />)]]
−
	OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5). GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.		OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5). GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.

@@ Line 18: / Line 18: @@
 [[File:图片4-5.png|left|thumb|200px|'''Figure 5''' OsCBL2 but not OsCBL1shows GA-specific up-regulation in embryoless rice half-grains. Total RNA was isolated from grains treated with ABA or GA (A) or no hormone (B) for the indicated times. Note that changes in mRNA abundance reflect changes occurring in the aleurone layer.(from reference<ref name="ref1" />)]]
 [[File:图片1-6.png|right|thumb|200px|'''Figure 6''' The rice calcineurin B-like gene OsCBL2 is up-regulated by GA treatment of rice aleurone layers. Transcript abundance of OsCBL2 (black circles) and actin (white circles) as measured by hybridization to a rice oligonucleotide chip (A). Total RNA was extracted from embryoless rice half-grains treated with GA, ABA, or no hormone for the indicated time. Expression of GA-induced α-amylase, RAmy1A (B), and ABA-induced dehydrin (C) genes in the same chip experiment are shown for comparison.(from reference<ref name="ref1" />)]]
 OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5).  GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.

@@ Line 19: / Line 19: @@
 [[File:图片1-6.png|right|thumb|200px|'''Figure 6''' The rice calcineurin B-like gene OsCBL2 is up-regulated by GA treatment of rice aleurone layers. Transcript abundance of OsCBL2 (black circles) and actin (white circles) as measured by hybridization to a rice oligonucleotide chip (A). Total RNA was extracted from embryoless rice half-grains treated with GA, ABA, or no hormone for the indicated time. Expression of GA-induced α-amylase, RAmy1A (B), and ABA-induced dehydrin (C) genes in the same chip experiment are shown for comparison.(from reference<ref name="ref1" />)]]
 [[File:图片3-7.png|left|thumb|200px|'''Figure 7''' OsCBL2 is expressed in many rice organs and at all stages of rice plant development. Data are pooled from individual microarray experiments where each radius in the figure represents a separate experiment. RNA samples were pooled prior to hybridization to the chip, and the data are presented as normalized intensity values.(from reference<ref name="ref1" />)]]
 OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5).  GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.

← Older revision		Revision as of 09:18, 13 June 2015
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	[[Category:Japonica Chromosome 12]]		[[Category:Japonica Chromosome 12]]
	[[Category:Chromosome 12]]		[[Category:Chromosome 12]]
		+
		+	==Structured Information==

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 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 18: / Line 18: @@
 [[File:图片4-5.png|leftt|thumb|200px|'''Figure 5''' OsCBL2 but not OsCBL1shows GA-specific up-regulation in embryoless rice half-grains. Total RNA was isolated from grains treated with ABA or GA (A) or no hormone (B) for the indicated times. Note that changes in mRNA abundance reflect changes occurring in the aleurone layer.(from reference<ref name="ref1" />)]]
 [[File:图片1-6.png|right|thumb|200px|'''Figure 6''' The rice calcineurin B-like gene OsCBL2 is up-regulated by GA treatment of rice aleurone layers. Transcript abundance of OsCBL2 (black circles) and actin (white circles) as measured by hybridization to a rice oligonucleotide chip (A). Total RNA was extracted from embryoless rice half-grains treated with GA, ABA, or no hormone for the indicated time. Expression of GA-induced α-amylase, RAmy1A (B), and ABA-induced dehydrin (C) genes in the same chip experiment are shown for comparison.(from reference<ref name="ref1" />)]]
-[[File:图片3-7.png|left|thumb|400px|'''Figure 7''' OsCBL2 is expressed in many rice organs and at all stages of rice plant development. Data are pooled from individual microarray experiments where each radius in the figure represents a separate experiment. RNA samples were pooled prior to hybridization to the chip, and the data are presented as normalized intensity values.(from reference<ref name="ref1" />)]]
+[[File:图片3-7.png|left|thumb|200px|'''Figure 7''' OsCBL2 is expressed in many rice organs and at all stages of rice plant development. Data are pooled from individual microarray experiments where each radius in the figure represents a separate experiment. RNA samples were pooled prior to hybridization to the chip, and the data are presented as normalized intensity values.(from reference<ref name="ref1" />)]]
-[[File:图片5-8.png|right|thumb|400px|'''Figure 8''' OsCBLs are expressed in rice seedling tissues. Total RNA was isolated from scutella, shoots, and roots of 1-week-old rice seedlings. RNA blots were probed with gene-specific probes for OsCBL1 to3. Hybridization to actin was used as a loading control.(from reference<ref name="ref1" />)]]
+[[File:图片5-8.png|right|thumb|200px|'''Figure 8''' OsCBLs are expressed in rice seedling tissues. Total RNA was isolated from scutella, shoots, and roots of 1-week-old rice seedlings. RNA blots were probed with gene-specific probes for OsCBL1 to3. Hybridization to actin was used as a loading control.(from reference<ref name="ref1" />)]]
-[[File:图片6-9.png|left|thumb|400px|'''Figure 9''' Expression of OsCBL2 in wild-type rice grain is higher than expression in d1 mutant grain. Total RNA was extracted from embryoless wild-type rice grain or d1mutant grain treated with 0.1 or 5 μM GA for 0, 3, or 8 h. RNA abundance of OsCBL2was determined using microarray (A and B) or northern (C) analysis. The abundance of rice RAmy1Awas also determined using the microarray (B).(from reference<ref name="ref1" />)]]
+[[File:图片6-9.png|left|thumb|200px|'''Figure 9''' Expression of OsCBL2 in wild-type rice grain is higher than expression in d1 mutant grain. Total RNA was extracted from embryoless wild-type rice grain or d1mutant grain treated with 0.1 or 5 μM GA for 0, 3, or 8 h. RNA abundance of OsCBL2was determined using microarray (A and B) or northern (C) analysis. The abundance of rice RAmy1Awas also determined using the microarray (B).(from reference<ref name="ref1" />)]]
 OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5).  GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.

@@ Line 4: / Line 4: @@
 [[File:Table1.jpg|left|thumb|200px|'''Table1''' Amino acid similarity and identity of rice CBLs (OsCBL1–10) and Arabidopsis CBLs (AtCBL1–10).    For each pairwise comparison, similarity values are followed by identity values in parentheses.(from reference<ref name="ref1" />)]]
 [[File:图片1.png|left|thumb|200px|'''Figure 1''' Yeast two-hybrid analysis demonstrates an interaction between OsCBLs and AtCIPKs. OsCBLs and AtCIPKs were translationally fused to the GAL4 DNA-binding domain (BD) and activation domain (AD) as indicated. Nutritional reporter systems minus Leu plus Trp (−LT) and minus Leu, Trp, and His (−LHT) and filter-lift GAL assays were employed to examine the interaction between OsCBLs and AtCIPKs (A). A positive control showing the interaction of AtCBL1 with AtCIPK1 is shown in B.(from reference<ref name="ref1" />)]]
-[[File:图片2.png|left|thumb|200px|'''Figure 2''' OsCBL2 to 4 are localized to membranes. OsCBL1 to 4 were translationally fused to GFP and transiently expressed in barley aleurone protoplasts. The figure shows representative epifluorescence images (top) and bright-field images (bottom) of single, transformed cells. The unmagnified width of each image is approximately 40 μm.(from reference<ref name="ref1" />)]]
+[[File:图片2.png|right|thumb|200px|'''Figure 2''' OsCBL2 to 4 are localized to membranes. OsCBL1 to 4 were translationally fused to GFP and transiently expressed in barley aleurone protoplasts. The figure shows representative epifluorescence images (top) and bright-field images (bottom) of single, transformed cells. The unmagnified width of each image is approximately 40 μm.(from reference<ref name="ref1" />)]]
 [[File:图片970009-3.png|left|thumb|200px|'''Figure 3''' Antisense OsCBL2 or HvCBL2delays the GA-induced vacuolation of barley aleurone protoplasts. Barley protoplasts were cotransfected with GFP andAsOsCBL2, GFP, andAsHvCBL2, or with GFP and empty cassette (pLZUbi) using the constructs diagrammed in A. The extent of vacuolation for individual protoplasts was scored using the five categories indicated in B. Vacuoles are seen as dark regions surrounded by bright regions of cytoplasm. The number of protoplasts in each category 48 h after transfection and 42 h after treatment with GA are shown in C forAsOsCBL2 and in D for AsHvCBL2.(from reference<ref name="ref1" />)]]
-[[File:图片10-4.gif|left|thumb|200px|'''Figure 4''' Antisense OsCBL2 does not delay GA-induced transcription of GUS from anα-amylase promoter in rice half-grain. A diagram of the constructs introduced by particle bombardment is shown in A. Transcription of GUS from a GA-regulated α-amylase promoter was measured relative to expression of LUX (GUS:LUX ratio) driven by a constitutive ubiquitin promoter (B). Half-grains were incubated for 24 h without hormone (−GA) or with GA and the ratio of GUS-to-LUX expression determined in the presence and absence of the antisense construct.(from reference<ref name="ref1" />)]]
+[[File:图片10-4.gif|right|thumb|200px|'''Figure 4''' Antisense OsCBL2 does not delay GA-induced transcription of GUS from anα-amylase promoter in rice half-grain. A diagram of the constructs introduced by particle bombardment is shown in A. Transcription of GUS from a GA-regulated α-amylase promoter was measured relative to expression of LUX (GUS:LUX ratio) driven by a constitutive ubiquitin promoter (B). Half-grains were incubated for 24 h without hormone (−GA) or with GA and the ratio of GUS-to-LUX expression determined in the presence and absence of the antisense construct.(from reference<ref name="ref1" />)]]
 Many developmental and environmental signals are transduced through changes in intracellular calcium concentrations. Calcineurin B-like (CBL) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements. RNA profiling using a rice (Oryza sativa cv Nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains. This analysis showed that a putative rice CBL gene responded to gibberellic acid, but not abscisic acid, treatment. The CBL gene family in rice contains at least 10 genes and these have extensive similarity to the CBLs of Arabidopsis (Arabidopsis thaliana). In yeast (Saccharomyces cerevisiae) two-hybrid assays, rice CBLs interact with the kinase partners of Arabidopsis CBLs. Only one rice CBL gene, OsCBL2, is up-regulated by GA in the aleurone layer.<ref name="ref1" />
@@ Line 16: / Line 16: @@
 ===Expression===
-[[File:图片4-5.png|right|thumb|200px|'''Figure 5''' OsCBL2 but not OsCBL1shows GA-specific up-regulation in embryoless rice half-grains. Total RNA was isolated from grains treated with ABA or GA (A) or no hormone (B) for the indicated times. Note that changes in mRNA abundance reflect changes occurring in the aleurone layer.(from reference<ref name="ref1" />)]]
+[[File:图片4-5.png|leftt|thumb|200px|'''Figure 5''' OsCBL2 but not OsCBL1shows GA-specific up-regulation in embryoless rice half-grains. Total RNA was isolated from grains treated with ABA or GA (A) or no hormone (B) for the indicated times. Note that changes in mRNA abundance reflect changes occurring in the aleurone layer.(from reference<ref name="ref1" />)]]
 [[File:图片1-6.png|right|thumb|200px|'''Figure 6''' The rice calcineurin B-like gene OsCBL2 is up-regulated by GA treatment of rice aleurone layers. Transcript abundance of OsCBL2 (black circles) and actin (white circles) as measured by hybridization to a rice oligonucleotide chip (A). Total RNA was extracted from embryoless rice half-grains treated with GA, ABA, or no hormone for the indicated time. Expression of GA-induced α-amylase, RAmy1A (B), and ABA-induced dehydrin (C) genes in the same chip experiment are shown for comparison.(from reference<ref name="ref1" />)]]
-[[File:图片3-7.png|right|thumb|400px|'''Figure 7''' OsCBL2 is expressed in many rice organs and at all stages of rice plant development. Data are pooled from individual microarray experiments where each radius in the figure represents a separate experiment. RNA samples were pooled prior to hybridization to the chip, and the data are presented as normalized intensity values.(from reference<ref name="ref1" />)]]
+[[File:图片3-7.png|left|thumb|400px|'''Figure 7''' OsCBL2 is expressed in many rice organs and at all stages of rice plant development. Data are pooled from individual microarray experiments where each radius in the figure represents a separate experiment. RNA samples were pooled prior to hybridization to the chip, and the data are presented as normalized intensity values.(from reference<ref name="ref1" />)]]
 [[File:图片5-8.png|right|thumb|400px|'''Figure 8''' OsCBLs are expressed in rice seedling tissues. Total RNA was isolated from scutella, shoots, and roots of 1-week-old rice seedlings. RNA blots were probed with gene-specific probes for OsCBL1 to3. Hybridization to actin was used as a loading control.(from reference<ref name="ref1" />)]]
-[[File:图片6-9.png|right|thumb|400px|'''Figure 9''' Expression of OsCBL2 in wild-type rice grain is higher than expression in d1 mutant grain. Total RNA was extracted from embryoless wild-type rice grain or d1mutant grain treated with 0.1 or 5 μM GA for 0, 3, or 8 h. RNA abundance of OsCBL2was determined using microarray (A and B) or northern (C) analysis. The abundance of rice RAmy1Awas also determined using the microarray (B).(from reference<ref name="ref1" />)]]
+[[File:图片6-9.png|left|thumb|400px|'''Figure 9''' Expression of OsCBL2 in wild-type rice grain is higher than expression in d1 mutant grain. Total RNA was extracted from embryoless wild-type rice grain or d1mutant grain treated with 0.1 or 5 μM GA for 0, 3, or 8 h. RNA abundance of OsCBL2was determined using microarray (A and B) or northern (C) analysis. The abundance of rice RAmy1Awas also determined using the microarray (B).(from reference<ref name="ref1" />)]]
 OsCBL2 high expression in booting culms, young spikes, seedling roots and shoots. Expression of OsCBL2 is not induced by salt, drought, cold or ABA treatment. Although both OsCBL1 and 2 were expressed in rice half-grains, OsCBL2 was specifically up-regulated by GA (Fig. 5).  GeneChip and RNA blotting experiments showed that OsCBL2 was most strongly expressed in aleurone and root and, using an expression intensity value of 50 as a cutoff, it is clear thatOsCBL2 is expressed in most tissues of the rice plant.