OsRPA2 - Revision history

July: /* Structured Information */

2014-06-06T01:13:08Z

‎Structured Information

July: /* Annotated Information */

2014-06-05T15:51:08Z

‎Annotated Information

July at 02:22, 5 June 2014

2014-06-05T02:22:55Z

July at 15:39, 27 May 2014

2014-05-27T15:39:03Z

July at 15:37, 27 May 2014

2014-05-27T15:37:47Z

July at 16:09, 26 May 2014

2014-05-26T16:09:22Z

July: Created page with "Please input one-sentence summary here. OsRPA2, the 32-kDa subunit of RPA from rice (Oryza sativa L.). ==Annotated Information== === Function Replication protein A (RPA) is a..."

2014-05-26T16:05:58Z

Created page with "Please input one-sentence summary here. OsRPA2, the 32-kDa subunit of RPA from rice (Oryza sativa L.). ==Annotated Information== === Function Replication protein A (RPA) is a..."

New page

Please input one-sentence summary here.
OsRPA2, the 32-kDa subunit of RPA from rice (Oryza sativa L.).
==Annotated Information==
===
Function

Replication protein A (RPA) is a heterotrimeric, singlestranded DNA-binding protein with several functions in DNA metabolism in humans and yeast and supposedly
also in plants. The plant-speciﬁc mechanisms of regulation have evolved for RPA2 within the generally well-conserved process of DNA replication, suggesting
speciﬁc requirements for regulation of DNA metabolism in plants as compared to other eukaryotes.
===Expression===
Replication protein A (RPA) is a heterotrimeric protein complex, encoded by RPA1, RPA2 and RPA3.The respective proteins in humans were termed RPA70,RPA32 and RPA14 according to their molecular weights. Even though the precise function of RPA is still not clear, extensive, mostly biochemical analysis of RPA proteins in humans and yeast led to the conclusion that RPA plays essential roles in many aspects of nucleic acid metabolism, including DNA replication, but also DNA repair and DNA recombination .RPA binds single-stranded DNA during replication to stabilize DNA in the single-strand conformation(Treuner et al. 1996). It stimulates DNA synthesisthrough interaction with DNA polymerase a (Kennyet al. 1990). Mutations in RPA2 were found to be lethal in yeast(Philipova et al. 1996). The RPA32 protein isphosphorylated at the G1- to S-phase transition (Fang and Newport 1993) and is hyperphosphorylated after DNA damage. Hyperphosphorylation of RPA32 possibly results in disassembly of the trimeric complex(Treuner et al. 1999 a, 1999b).Much less is known about RPA from plants. An RPA protein complex was isolated from tobacco by binding and elution from single-stranded DNA and was shown to induce DNA polymerase activity (Garcia-Maya and Buck 1997). From rice, cDNAs of the two subunits OsRPA1 (van der Knaap et al. 1997; Ishibashi et al.2001) and OsRPA2 (Ishibashi et al. 2001; this study)
were isolated. The OsRPA1 gene was shown to be expressed mainly in proliferative tissues and was induced coordinately with cell cycle induction by gibberellic acid(van der Knaap 1997) and during re-growth after starvation of suspension cells (Ishibashi et al. 2001). A gene,coding for the RPA3 subunit has not been identiﬁed from plants to date.We describe here, the gene and protein regulation of OsRPA2 from rice. The results presented indicate that despite a high degree of conservation of RPA among eukaryotes, regulation of OsRPA2 displays features that appear to be speciﬁc to plants
===Evolution===
A partial cDNA of 335 nt comprising the 3¢-untranslated region(UTR) and 80 nt of the coding sequence at the C-terminus was isolated by subtractive hybridization as described by Lorbiecke and Sauter (2002). Through PCR from a rice cDNA library (Sauter et al. 1995) using a gene-specific primer (bp 896–915) and a plasmid-derived primer (bp 798–817 of pBluescript II SK) ,a longer clone was obtained that included the coding region up to the start codon. To obtain the 5¢-UTR of the cDNA, a 5¢-rapid amplification of cDNA ends(5¢-RACE; Gibco, Karlsruhe,Germany) was performed using cDNA as template that was reverse-transcribed from total RNA isolated from the intercalary meristem of plants that were submerged for 6 h. The complete sequence with the encoded open reading frame was termed OsRPA2and was submitted to the nucleotide sequence database at EMBL (accession number AJ278822).

You can also add sub-section(s) at will.

==Labs working on this gene==
Department of Applied Biological Science,Faculty of Science and Technology,
Science University of Tokyo, 2641 Yamazaki,278-8510 Noda-shi, Chiba-ken, Tokyo, Japan

==References==
[1] Plant-specific regulation of replication protein A2 (OsRPA2) from rice during the cell cycle and in response to ultraviolet light exposure
Planta, 2003, 217(3): 457-465.
[2]Wold MS (1997) Replication protein A: a heterotrimeric, singlestranded DNA-binding protein required for eukaryotic DNA metabolism. Annu Rev Biochem 66:61–92.

[3]Treuner K, Ramsperger U, Knippers R (1996) Replication protein A induces the unwinding of long double-stranded DNA regions. J Mol Biol 259:104–112.

==Structured Information==
{{JaponicaGene|

Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:36526606..36529420|
CDS = 36526967..36527051,36527165..36527233,36527599..36527681,36527762..36527857,36527983..36528061<br>,36528143..36528220,36528357..36528398,36528803..36528900,36528996..36529115<br>,36529208..36529297|
GCID = <gbrowseImage1>
name=NC_008395:36526606..36529420
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:36526606..36529420
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgatgtccttcagccagcccgacgccttctcgccgtcgcagttcacctcctcccagaatgccgccgccgactctaccaccccttccaagagccggggcgcatcgagcaccatgccgctcaccgtgaagcagatctctgaagcgcagcaatctggcatcaccggggagaagggcgctcccttcgtcgtcgacggcgtcgagacagccaacgttaggcttgtgggattggtcagtggcaagacggagcggaacaccgatgtgtccttcacgatcgacgatggcaccggccgcctcgatttcatcagatgggtcaatgatggtgctgattctgctgaaaccgctgctgttcagaatggaatgtatgtttctgtcattggtagcctcaaggggcttcaggagaggaagcgtgctactgctttcgctatcaggcctgtaactgattataatgaggtcacactgcatttcattcagtgcgtgcgaatgcatttagagaacaccaaatcacagattggtagccctgcaaaaacatattctgccatgggatcctcatcttccaatggcttcagtgaaatgaccactccgacctctgtgaaatccaacccggcaccagtgctcagtgtaacaaatggatcaaaaactgatttgaacacagaagtgctgaatgttttccgcgaaccagcaaacgtcgagagcgagcatggtgtgcacattgatgaaatcgtgaagcgcttcaggctaccggaggccaagatcaaggtagctattgattacctcgctgacattgggcacatatactcaacaatcgacgagtctcactacaagtcagcgttcaatgaataa</cdnaseq>|
AA = <aaseq>MMSFSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQ ISEAQQSGITGEKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIR WVNDGADSAETAAVQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVR MHLENTKSQIGSPAKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTE VLNVFREPANVESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSA FNE</aaseq>|
DNA = <dnaseqindica>2370..2454#2188..2256#1740..1822#1564..1659#1360..1438#1201..1278#1023..1064#521..618#306..425#124..213#ctttttagtctactcgatctaactccaaatcccatcaaccccgccgccgccgccgccgccgccaccaccgcgagacgcgagaggagagtgaaagaaagattcagagagagagagagagtaagcatgatgtccttcagccagcccgacgccttctcgccgtcgcagttcacctcctcccagaatgccgccgccgactctaccaccccttccaaggtagtaatctccattccattctcatccgtgtgtgtcttggtttcgagaaacaagggggataaaatcatctttcttttcttttgattttgtagagccggggcgcatcgagcaccatgccgctcaccgtgaagcagatctctgaagcgcagcaatctggcatcaccggggagaagggcgctcccttcgtcgtcgacggcgtcgagacagccaacgtatgctgcaacacaacacggaaaatgccccccccctccctgctttaatttcctcttagcttgttgatttgggtgtttccttgttgctttttcaggttaggcttgtgggattggtcagtggcaagacggagcggaacaccgatgtgtccttcacgatcgacgatggcaccggccgcctcgatttcatcagatggtgagcccatttcgccgattcaatcgcttctgtatcttcctagtcaatactatttctgcttcaactgccatcactggttgtttactactacttgtagttcagcttgctcgttctagacttccaaacaattcaaggccgggaattcggaaaatgttttttcaatcctgaatgctaattactactagtataaacattctactacaaacggtgtcttttggtttctagaagcattggttgagcatcagcacatatctggtctgtcttttcgtattcaaggcaatgatataagtagtactcctacatctttagttctgaaattaggttaattactacaacatcctgttgttcttttgccaatggcttcagcagatttacatggttatttatctccatccattttacagggtcaatgatggtgctgattctgctgaaaccgctgctgttcagtacgtaccagtcatagctcccatttatgcttctatatatgcacttcatcatcttttatttttgaggattgttagcatctttctccttgaggatcatcaccttttctcataactctattttttttcctaattaaaggaatggaatgtatgtttctgtcattggtagcctcaaggggcttcaggagaggaagcgtgctactgctttcgctatcaggtacgtaacattcatatattctgctggtgcaaatatgcaataagacatgcaactaattttctcttattttgttcatttcaggcctgtaactgattataatgaggtcacactgcatttcattcagtgcgtgcgaatgcatttagagaacaccaaatcacaggtatgctgctgtgatgtacaattgttctatacaaatactctcgtgatagcaatctcgtgttcattagttagaaggttcatgagtttgaacaaaaaaagaactaatgcttaccttcaacactgtagattggtagccctgcaaaaacatattctgccatgggatcctcatcttccaatggcttcagtgaaatgaccactccgacctctgtgaaatccaacccggtaagttgtatttacagttattgcattgatcaaacaatcaaactcctgtgctgccgtgatttattccctcatccttacaggcaccagtgctcagtgtaacaaatggatcaaaaactgatttgaacacagaagtgctgaatgttttccgcgaaccagcaaacgtgtaagttgcacatccgtcttccttgtcatctaggaaaaagaaatgcttgtcctgttaaagacttaaagtcaattactcaggaatgaattccgaatgcatgtctctgtttcttttgcctttttttatctgcttagaaataccagctcccgtgaaaacctgatgctccatcaatatctacttttcctagattttccaattaaacacaagtagaattggctaggattgctagctaggagaaatggagagtatttgtctgttaatattgtcgtgttggctgaagaagcggcctaccgattgtattaagatttgaggatctggtgactgctgttttgctcatcttgtttgaccaacgaataaactgacagcgagagcgagcatggtgtgcacattgatgaaatcgtgaagcgcttcaggctaccggaggccaagatcaagtctgtatttcactccttcgctcacacacacacacgcaacagcgagacattaattcgacgaaaaattatggctgctggttgatgcttacctcgctctctgttaatttatgcagggtagctattgattacctcgctgacattgggcacatatactcaacaatcgacgagtctcactacaagtcagcgttcaatgaataaaggggattgaagcctcagttacttactagtatgtatcctctagcctcgaagctggctgcagctgaaactgaagaacaatcgagatgcttcatgtttgcttgtcatactaaccagtgtgcgtttgttttgctccatccagtgaaattacatgtaaccgtatctgtattacgagtagcacatatcgagtgacaaaacatataatatatgtatgtttgttgttttgcagcatcagcattatttggactgtaacatgcttgattgatctctttggatgcagttaatttcattgtgatattaactcacgcatcatcgatcgatcgtgtgttttttggtagctagatggtccaatcacaataatctg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055139.1 RefSeq:Os02g0829100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

@@ Line 38: / Line 38: @@
 name=OsRPA2
-source=RiceChromosome 5'-untranslated region
+source=Rice Chromosome2
 preset=GeneLocation
 name=OsRPA2
-source=RiceChromosome 5'-untranslated region
+source=Rice Chromosome2
 preset=GeneLocation

@@ Line 5: / Line 5: @@
 ===Function
 Replication protein A (RPA) is a heterotrimeric, singlestranded DNA-binding protein with several functions in DNA metabolism in humans and yeast and supposedly
 also in plants. OsRPA2, the 32-kDa subunit of RPA from rice (Oryza sativa L.).The plant-speciﬁc mechanisms of regulation have evolved for RPA2 within the generally well-conserved process of DNA replication, suggesting speciﬁc requirements for regulation of DNA metabolism in plants . Even though the precise function of RPA is still not clear, extensive, mostly biochemical analysis of RPA proteins in humans and yeast led to the conclusion that RPA plays essential roles in many aspects of nucleic acid metabolism, including DNA replication, but also DNA repair and DNA recombination .
 ===Expression===
 Replication protein A (RPA) is a heterotrimeric protein complex, encoded by RPA1, RPA2 and RPA3.RPA binds single-stranded DNA during replication to stabilize DNA in the single-strand conformation. It stimulates DNA synthesisthrough interaction with DNA polymerase α. Mutations in RPA2 were found to be lethal in yeast(Philipova et al. 1996). Much less is known about RPA from plants. An RPA protein complex was isolated from tobacco by binding and elution from single-stranded DNA and was shown to induce DNA polymerase activity (Garcia-Maya and Buck 1997). From rice, cDNAs of the two subunits OsRPA1 (van der Knaap et al. 1997; Ishibashi et al.2001) and OsRPA2 (Ishibashi et al. 2001; this study) were isolated. The OsRPA1 gene was shown to be expressed mainly in proliferative tissues and was induced coordinately with cell cycle induction by gibberellic acid(van der Knaap 1997) and during re-growth after starvation of suspension cells (Ishibashi et al. 2001). The results of the gene and protein regulation of OsRPA2 from rice presented indicate that despite a high degree of conservation of RPA among eukaryotes, regulation of OsRPA2 displays features that appear to be speciﬁc to plants.In rice, OsRPA2 expression was regulated at the level of transcription as well as at the protein level. Regulation of OsRPA2 protein abundance appeared to occur at more than one level since induction of gene expression during S phase lagged behind elevated protein levels.
 ===Evolution===
   Through PCR from a rice cDNA library (Sauter et al. 1995) using a gene-specific primer (bp 896–915) and a plasmid-derived primer (bp 798–817 of pBluescript II SK) ,a longer clone was obtained that included the coding region up to the start codon. To obtain the 5'-UTR of the cDNA, a 5'-rapid amplification of cDNA ends(5'-RACE; Gibco, Karlsruhe,Germany) was performed  using cDNA as template that was reverse-transcribed from total RNA isolated from the intercalary meristem of plants that were submerged for 6 h. The complete sequence with the encoded open reading frame was termed OsRPA2and was submitted to the nucleotide sequence database at EMBL (accession number AJ278822).

@@ Line 1: / Line 1: @@
 ==Annotated Information==
 ===Function
 ===Expression===
-Replication protein A (RPA) is a heterotrimeric protein complex, encoded by RPA1, RPA2 and RPA3.The respective proteins in humans were termed RPA70,RPA32 and RPA14 according to their molecular weights. Even though the precise function of RPA is still not clear, extensive, mostly biochemical analysis of RPA proteins in humans and yeast led to the conclusion that RPA plays essential roles in many aspects of nucleic acid metabolism, including DNA replication, but also DNA repair and DNA recombination .RPA binds single-stranded DNA during replication to stabilize DNA in the single-strand conformation(Treuner et al. 1996). It stimulates DNA synthesisthrough interaction with DNA polymerase a (Kennyet al. 1990). Mutations in RPA2 were found to be lethal in yeast(Philipova et al. 1996). The RPA32 protein isphosphorylated at the G1- to S-phase transition (Fang and Newport 1993) and is hyperphosphorylated after DNA damage. Hyperphosphorylation of RPA32 possibly results in disassembly of the trimeric complex(Treuner et al. 1999 a, 1999b).Much less is known about RPA from plants. An RPA protein complex was isolated from tobacco by binding and elution from single-stranded DNA and was shown to induce DNA polymerase activity (Garcia-Maya and Buck 1997). From rice, cDNAs of the two subunits OsRPA1 (van der Knaap et al. 1997; Ishibashi et al.2001) and OsRPA2 (Ishibashi et al. 2001; this study)
+Replication protein A (RPA) is a heterotrimeric protein complex, encoded by RPA1, RPA2 and RPA3.RPA binds single-stranded DNA during replication to stabilize DNA in the single-strand conformation. It stimulates DNA synthesisthrough interaction with DNA polymerase α. Mutations in RPA2 were found to be lethal in yeast(Philipova et al. 1996). Much less is known about RPA from plants. An RPA protein complex was isolated from tobacco by binding and elution from single-stranded DNA and was shown to induce DNA polymerase activity (Garcia-Maya and Buck 1997). From rice, cDNAs of the two subunits OsRPA1 (van der Knaap et al. 1997; Ishibashi et al.2001) and OsRPA2 (Ishibashi et al. 2001; this study) were isolated. The OsRPA1 gene was shown to be expressed mainly in proliferative tissues and was induced coordinately with cell cycle induction by gibberellic acid(van der Knaap 1997) and during re-growth after starvation of suspension cells (Ishibashi et al. 2001). The results of the gene and protein regulation of OsRPA2 from rice presented indicate that despite a high degree of conservation of RPA among eukaryotes, regulation of OsRPA2 displays features that appear to be speciﬁc to plants.In rice, OsRPA2 expression was regulated at the level of transcription as well as at the protein level. Regulation of OsRPA2 protein abundance appeared to occur at more than one level since induction of gene expression during S phase lagged behind elevated protein levels.
-were isolated. The OsRPA1 gene was shown to be expressed mainly in proliferative tissues and was induced coordinately with cell cycle induction by gibberellic acid(van der Knaap 1997) and during re-growth after starvation of suspension cells (Ishibashi et al. 2001). A gene,coding for the RPA3 subunit has not been identiﬁed from plants to date.We describe here, the gene and protein regulation of OsRPA2 from rice. The results presented indicate that despite a high degree of conservation of RPA among eukaryotes, regulation of OsRPA2 displays features that appear to be speciﬁc to plants
 ===Evolution===
-A partial cDNA of 335 nt comprising the 3¢-untranslated region(UTR) and 80 nt of the coding sequence at the C-terminus was isolated by subtractive hybridization as described by Lorbiecke and Sauter (2002). Through PCR from a rice cDNA library (Sauter et al. 1995) using a gene-specific primer (bp 896–915) and a plasmid-derived primer (bp 798–817 of pBluescript II SK) ,a longer clone was obtained that included the coding region up to the start codon. To obtain the 5¢-UTR of the cDNA, a 5¢-rapid amplification of cDNA ends(5¢-RACE; Gibco, Karlsruhe,Germany) was performed  using cDNA as template that was reverse-transcribed from total RNA isolated from the intercalary meristem of plants that were submerged for 6 h. The complete sequence with the encoded open reading frame was termed OsRPA2and was submitted to the nucleotide sequence database at EMBL (accession number AJ278822).
+ Through PCR from a rice cDNA library (Sauter et al. 1995) using a gene-specific primer (bp 896–915) and a plasmid-derived primer (bp 798–817 of pBluescript II SK) ,a longer clone was obtained that included the coding region up to the start codon. To obtain the 5'-UTR of the cDNA, a 5'-rapid amplification of cDNA ends(5'-RACE; Gibco, Karlsruhe,Germany) was performed  using cDNA as template that was reverse-transcribed from total RNA isolated from the intercalary meristem of plants that were submerged for 6 h. The complete sequence with the encoded open reading frame was termed OsRPA2and was submitted to the nucleotide sequence database at EMBL (accession number AJ278822).
 You can also add sub-section(s) at will.
@@ Line 29: / Line 31: @@
 ==Structured Information==
-{{JaponicaGene|
+{{Oryza Gene|
 name=OsRPA2
-source=RiceChromosome02
+source=RiceChromosome 5'-untranslated region
 preset=GeneLocation
-</gbrowseImage1>|
 name=OsRPA2
-source=RiceChromosome02
+source=RiceChromosome 5'-untranslated region
 preset=GeneLocation
-</gbrowseImage2>|
 CDNA =
-AA = <aaseq>MMSFSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQ                     ISEAQQSGITGEKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIR                     WVNDGADSAETAAVQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVR                     MHLENTKSQIGSPAKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTE                     VLNVFREPANVESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSA                     FNE</aaseq>|
+AA = <aaseq>---------MNSFSQPDAFSPSQ---FTSSQNAAADSTTPSKSRGAS--;35
 DNA =
 Link = http://www.ricedata.cn/gene/list/1988.htm
 }}
 [[Category:Genes]]
-[[Category:Japonica mRNA]]
+[[Category:Oryza cDNA]]
-[[Category:Oryza Sativa Japonica Group]]
+[[Category:Oryza Sativa ]]