RiceWiki:Users - Revision history

Leilei Yang at 17:05, 10 June 2014

2014-06-10T17:05:13Z

Leilei Yang at 17:04, 10 June 2014

2014-06-10T17:04:56Z

Leilei Yang at 17:03, 10 June 2014

2014-06-10T17:03:28Z

Leilei Yang at 17:03, 10 June 2014

2014-06-10T17:03:09Z

Leilei Yang at 16:34, 10 June 2014

2014-06-10T16:34:38Z

Leilei Yang at 16:29, 10 June 2014

2014-06-10T16:29:04Z

Leilei Yang at 16:19, 10 June 2014

2014-06-10T16:19:33Z

Leilei Yang: Created page with "The gene ''Oryza sativa indoleacetic acid 13(OsIAA13)'' is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in ri..."

2014-06-10T16:12:51Z

Created page with "The gene ''Oryza sativa indoleacetic acid 13(OsIAA13)'' is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in ri..."

New page

The gene ''Oryza sativa indoleacetic acid 13(OsIAA13)'' is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice.
The open reading frame (ORF) length of OsIAA13 was 2116 bp. It's located on the long arm of chromosome 3.

A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13.
OsIAA13 encodes a member of the rice Aux/IAA protein family and is believed to play an essential part in blocking auxin signaling by repressing ARF function as a transcriptional regulator.

← Older revision		Revision as of 17:05, 10 June 2014
Line 47:		Line 47:

	[1]Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122		[1]Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
		+
	[2]M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.		[2]M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.

@@ Line 7: / Line 7: @@
 '''''OsIAA13 (Os03g0742900)'' sequence'''
 Position: chr03:31291676..31294263 (- strand)
 Length:	2588 bp
@@ Line 44: / Line 46: @@
 TGAGACTATT ATTATTATGG TATCTGGT
-[[1]]Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
+[1]Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
-[[2]]M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.
+[2]M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.

@@ Line 9: / Line 9: @@
 Position: chr03:31291676..31294263 (- strand)
 Length:	2588 bp
 GGCTCCTCTC TCTTCTTCCA ACACCTCCTA ATCACCACAA AGGCTTAAAA ATCTCCAGTC TCCATAGAGA GAAAGAGAGA
 GAAGAGAAGG CAGCTTGATT TGGGGCGAGG AGACGCGATA GCCGCGATAG GCTAAGCAGC TGGCAGCGGC GGCTGCTGCG

@@ Line 1: / Line 1: @@
-The gene ''Oryza sativa indoleacetic acid 13(OsIAA13)'' is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice[[1]].
+The gene ''Oryza sativa indoleacetic acid 13(OsIAA13)'' is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice[1].
 The open reading frame (ORF) length of OsIAA13 was 2116 bp. It's located on the long arm of chromosome 3.
-A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13.
+A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13[1].
 OsIAA13 encodes a member of the rice Aux/IAA protein family and is believed to play an essential part in blocking auxin signaling by repressing ARF function as a transcriptional regulator.
-The gain-of-function mutant OsIAA13 shares high sequence similarity with OsIAA11 and OsIAA30 in rice, and with AXR2/IAA7, SLR/IAA14, IAA16, and AXR3/IAA17 in ''Arabidopsis''[[2]]. Osiaa11 showed a similar phenotype to Osiaa13: the lateral root number of Osiaa11 was reduced because of a defect in LRP initiation but the crown root number was not reduced. Examination of the RiceXPro database revealed that ''OsIAA13'', ''OsIAA1''1, and ''OsIAA30'' show similar gene expression patterns indicating that the function of OsIAA30 might be redundant with OsIAA13 and OsIAA11 in lateral root initiation. ''OsIAA13'', ''OsIAA11'', and ''OsIAA30'' may play an important role in lateral root formation but not in crown root formation.
+The gain-of-function mutant OsIAA13 shares high sequence similarity with OsIAA11 and OsIAA30 in rice, and with AXR2/IAA7, SLR/IAA14, IAA16, and AXR3/IAA17 in ''Arabidopsis''[2]. Osiaa11 showed a similar phenotype to Osiaa13: the lateral root number of Osiaa11 was reduced because of a defect in LRP initiation but the crown root number was not reduced. Examination of the RiceXPro database revealed that ''OsIAA13'', ''OsIAA1''1, and ''OsIAA30'' show similar gene expression patterns indicating that the function of OsIAA30 might be redundant with OsIAA13 and OsIAA11 in lateral root initiation. ''OsIAA13'', ''OsIAA11'', and ''OsIAA30'' may play an important role in lateral root formation but not in crown root formation.
-[[1]].Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
+'''''OsIAA13 (Os03g0742900)'' sequence'''
-[[2]].M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.
+Position: chr03:31291676..31294263 (- strand)

@@ Line 6: / Line 6: @@
 The gain-of-function mutant OsIAA13 shares high sequence similarity with OsIAA11 and OsIAA30 in rice, and with AXR2/IAA7, SLR/IAA14, IAA16, and AXR3/IAA17 in ''Arabidopsis''[[2]]. Osiaa11 showed a similar phenotype to Osiaa13: the lateral root number of Osiaa11 was reduced because of a defect in LRP initiation but the crown root number was not reduced. Examination of the RiceXPro database revealed that ''OsIAA13'', ''OsIAA1''1, and ''OsIAA30'' show similar gene expression patterns indicating that the function of OsIAA30 might be redundant with OsIAA13 and OsIAA11 in lateral root initiation. ''OsIAA13'', ''OsIAA11'', and ''OsIAA30'' may play an important role in lateral root formation but not in crown root formation.
-.Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
+[[1]].Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
-.M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.
+[[2]].M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (''Oryza sativa''), Functional and Integrative Genomics 6 (2006) 47–59.

← Older revision		Revision as of 16:19, 10 June 2014
Line 4:		Line 4:
	A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13.		A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13.
	OsIAA13 encodes a member of the rice Aux/IAA protein family and is believed to play an essential part in blocking auxin signaling by repressing ARF function as a transcriptional regulator.		OsIAA13 encodes a member of the rice Aux/IAA protein family and is believed to play an essential part in blocking auxin signaling by repressing ARF function as a transcriptional regulator.
		+	[[File:]]