Difference between revisions of "Os02g0170300"

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'''GO assignment(s):''' GO:0003700, GO:0005634, GO:0043565
 
'''GO assignment(s):''' GO:0003700, GO:0005634, GO:0043565
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===Mutation===
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Figure 1 shows that during the first 6 days after flowering(DAF), there is no obvious difference between wide type line seed (ZH11) and A-MADS29 transgenic lines seeds (A-2, A-14, and A-33) in which the ''OsMADS29'' was suppressed. After 8 DAF, the A-MADS29 seeds become shrunken, and this phenotype persists until 30 DAF because of a defect in dry matter accumulation, whereas the seeds are fully filled with dry matter by 30 DAF in ZH11<ref name="ref1" />.
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[[File:RiceExpressionPattern.jpg|right|thumb|450px|'''Figure 2.''' ''Expression analyses of OsMADS29(from reference <ref name="ref2"/>).'']]
  
 
===Expression===
 
===Expression===

Revision as of 14:18, 16 March 2013

The rice OsMADS29 is a member of MADS-box transcription factors in rice, it belongs to class B genes of the ABC model of flower development in plants[1].

Annotated Information

Function

Figure 1. Seeds from wild type lines Vs transgenic lines (from reference) [1].

OsMADS29 plays an important role during seed development of rice and silencing of OsMADS29 Results in the seeds shrunken as well as a reduced grain-filling rate in rice[1][2]. It is a key regulator of the degradation of the nucellus and nucellar projection, which undergoing PCD during early rice seed development[1]. Down regulation of OsMADS29 does not affect ovule development but causes seeds to abort or shrivel as they are most likely deficient in endosperm development and starch accumulation[2].
After rice fertilization, the increased IAA content induces the expression of MADS29, which then stimulates the degradation of the nucellus and the nucellar projection by directly stimulating a Cys protease and NBS-LRR proteins. Acting through MADS29 or other pathways, auxin regulates the PCDprocesses required for normal endosperm development and grain filling[1].

GO assignment(s): GO:0003700, GO:0005634, GO:0043565

Mutation

Figure 1 shows that during the first 6 days after flowering(DAF), there is no obvious difference between wide type line seed (ZH11) and A-MADS29 transgenic lines seeds (A-2, A-14, and A-33) in which the OsMADS29 was suppressed. After 8 DAF, the A-MADS29 seeds become shrunken, and this phenotype persists until 30 DAF because of a defect in dry matter accumulation, whereas the seeds are fully filled with dry matter by 30 DAF in ZH11[1].

File:RiceExpressionPattern.jpg
Figure 2. Expression analyses of OsMADS29(from reference [2]).

Expression

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Evolution

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Labs working on this gene

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References

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Structured Information

Gene Name

Os02g0170300

Description

Similar to MADS box protein ZMM17

Version

NM_001052563.1 GI:115444496 GeneID:4328443

Length

4006 bp

Definition

Oryza sativa Japonica Group Os02g0170300, complete gene.

Source

Oryza sativa Japonica Group 

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 2

Location

Chromosome 2:3833127..3837132

Sequence Coding Region

3833796..3834074,3834202..3834243,3834352..3834393,3834894..3835055,3835193..3835262
,3836519..3836706

Expression

GEO Profiles:Os02g0170300

Genome Context

<gbrowseImage1> name=NC_008395:3833127..3837132 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008395:3833127..3837132 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atggggcgcggcaagatcgagatcaagaggatcgagaacgcgacgaacaggcaggtgacattctcgaagaggcggggagggctactgaagaaggcgaacgagctcgccgtgctctgcgacgcccgcgtcggcgtcgtcatcttctccagcaccggcaagatgttcgagtactgcagccctacctgcagtttgagggaactcatcgagcattaccagaccgtcaccaacactcatttcgaggagatcaaccacgatcagcaaatatttgtggagatgactcggatgaggaacgagatggagaagctggacggtggcatcaggaggttcaccggcgacgacctctccaacctcacgctcgccgacatcaacgatctcgagcagcagctcgaattctccgtcaccaaagtccgtgcgagaaagcatcagctcctgaaccagcagctggacaaccttcgtcgcaaggagcacatcctggaagaccagaacagcttcctgtgccgcatgatcaacgagaaccatcatcaggcggcggtgggcggcggcgacgtgaaggcgatggtggagatggcgccggtgctgtcgatgctgacggcggcgccggcgtactacggcgaggagtcgtcgagcaccgcgctgcagctcaccccgccgctgcacgccgtcgacgccgccgccgccgccgggttccggctgcagccgacgcagcccaacctgcaggaccccggctgcagcagcagcagcttccatgccgccgccgccggccacggcctgcagctgtggtaa</cdnaseq>

Protein Sequence

<aaseq>MGRGKIEIKRIENATNRQVTFSKRRGGLLKKANELAVLCDARVG VVIFSSTGKMFEYCSPTCSLRELIEHYQTVTNTHFEEINHDQQIFVEMTRMRNEMEKL DGGIRRFTGDDLSNLTLADINDLEQQLEFSVTKVRARKHQLLNQQLDNLRRKEHILED QNSFLCRMINENHHQAAVGGGDVKAMVEMAPVLSMLTAAPAYYGEESSSTALQLTPPL HAVDAAAAAGFRLQPTQPNLQDPGCSSSSFHAAAAGHGLQLW</aaseq>

Gene Sequence

<dnaseqindica>3059..3337#2890..2931#2740..2781#2078..2239#1871..1940#427..614#agcctttgaagcaagcttagcacttcacaaggtatgtacatagcacattactactcctgccctatctatctaccctgttcatgagtaattcaagaaaaaaaaaaggacaccttttctagctagttcttcatcctttcgcagcaaagagtttttacatctctagctactagttgttctcttctctacatgaaattttttctacttctgattgcaccctttacatagctgtggggggaaatccaatttgagcacccatgccttatgttgattttaacaaaattcgtccatgtatcgatatgttcataacttggccatttctgttcttcttcttcttcttcttcttcttcagtttcctcttgtgcagaagaaggagaaacaaaagaacacagaaattaaattaaactcgatcaggaaggtatagcaggcatggggcgcggcaagatcgagatcaagaggatcgagaacgcgacgaacaggcaggtgacattctcgaagaggcggggagggctactgaagaaggcgaacgagctcgccgtgctctgcgacgcccgcgtcggcgtcgtcatcttctccagcaccggcaagatgttcgagtactgcagccctacctgcaggtacggttcttgttttgttctcttatattatcagatgctgttcaatctttcctttctccagcttagtgtctcttgttctctttttagtgcttgatttgatgggttaattaatttcttgttttttgtcaaccaagagattaagttttcggctaagtttctctcggcctttattttgtttttcagagaaaattaactaactcatgcagtttctaccctttgttttctttgggcaagagtgtttaattatgatcatttgatctgttcatataatggatggatgccttgcatttattcttcatctgaactatttttcttgtcctgaactcctgatcgatcgagtttttcctaccttcattttaactattttcagtgtatatgtatgcatctgcagtttcctttgatatattttcgaagagccaagaatctattacaaaattaaatcatgatcaaaggcatacataagcaatgatctatatgccctagtgtctcttgtgacttgattacgcacttgtcttttacatctctgagcaacatatatgtatgtagatttgcaacttgatcctgaatttatagtcaactagttaattaatccttatttccctctttctaatgatttctaggatcgagctgggttttttaggtggatttccccaaaaaactatttcatatgtggctaatttaatcaaactaaaagatgtatatcaacagggaatataatttgaacaagtgtacgacagtacgaccatatcttatcttatgttatgcatgtttttatacttaaaaaaagatgcgaatataagagaatgacctaatattaaataattagaagaggtgaggtttcgaaccttggttgtctagtccaccactttgtggagctagccggaagatccccagacgtttctcacatgattttatactgttctgcagcatattatagttaattaatatgtgaatattcatgtgaagttctttcttccgatcccctgttcattcctgttattagttaagaaattttcgtttgtttcattttgatctaaaataatatactctctgtaagtatatacatcaccaagagaaaattctagctacaaaattaatttataccatctgatttgtacatcagagattgtcatttcttttttgaatgcgcaaaagaattacacatgcaccattccaattagatacatgaacttatcaaagtattaattaactcacaactaatcaaggtacttgtttcaatgtttgacaatatatagtttgagggaactcatcgagcattaccagaccgtcaccaacactcatttcgaggagatcaaccacgatcaggtatacattaattttacaatcagcaacacaatgtctgatcaatatcagaatatatatcgatctatctgccagcagttgaaatgaaattttctttcaaatgcactgatcaaatataattaatgtgcacatatatatagcaaatatttgtggagatgactcggatgaggaacgagatggagaagctggacggtggcatcaggaggttcaccggcgacgacctctccaacctcacgctcgccgacatcaacgatctcgagcagcagctcgaattctccgtcaccaaagtccgtgcgagaaaggtaattacttcctccatatcatattataagtcgttttaattttttttacaaatttatttaagtttaatcaagtttataaaaaatatattagtatttcaaaaacaaacattatcaaaatgtattcagtgatagatttaataaaattaatttggtattacatgtgttgctatttttttctataaatttaatcaaacttaaaagaaaaaaaattaaaatgacttgtaatatgaaatagagctagtaattgattaagaagatagtatttgatgaatgaatcattcttcagagttaatttagaatgatcagtctctgattcaatggtaaatggtaagtttagggtttagggattaattgatcccatagttttttcctcttttctgtaaagctttgctttcgtaagttttttattttgttatattcaatgaaataggtgtatctaagtgccgattcgttaaaaaaataaaaaatagttgtttcacattaattcttgcttgttgcagcatcagctcctgaaccagcagctggacaaccttcgtcgcaaggtaattaatttgcactactaaactgatcatgaaacaccaaatactttgctgtatgatgatgaaaaaaaaaatgattgattcaattctatgcatgaaattgatgaacaggagcacatcctggaagaccagaacagcttcctgtgccgcatggtatacgtatagttagttaatcagtaatcaagaattcattaaaatgttttgattcttgaagcacatatatgtacattaattatggtgtttggttgattcttgtacatgaaactatatatgaattcagatcaacgagaaccatcatcaggcggcggtgggcggcggcgacgtgaaggcgatggtggagatggcgccggtgctgtcgatgctgacggcggcgccggcgtactacggcgaggagtcgtcgagcaccgcgctgcagctcaccccgccgctgcacgccgtcgacgccgccgccgccgccgggttccggctgcagccgacgcagcccaacctgcaggaccccggctgcagcagcagcagcttccatgccgccgccgccggccacggcctgcagctgtggtaagaacaaacgaagcaaagcatacatggcacatcctgatactctcatcaggtgtgtgatgtgaatatatatatcgcaatgattttatctactagtttatgtacttcatccgttttataatataagtcattctagtatttttcatattcatattgatattaatgaatacatatatatatcaatataaatgtaggaaatgctagaataacttacattgtgaaacggagggagtataattcatcgcaacacagctgcgaattaatcatgcattgatatcattctgcagagatctagatgattgcttgctaattaagcacatgatggcattgtcgattctgggagctaggagtaacttggagaccatgaagatgcatgatcagctgatgatagacgatcgagttgggttgctgtgttgttcaattgttcatcgttctggacatatatatatatatatatgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctaattagcacatcagaataatcaagcttttctacctagttaactagtacagctgctaagtaccttttgatttgttggtgtgtgaactactgaactggagttggatattaaggtctccatgcatttgcatggttaattagcagagagatcattgaggtttttaggatgtaac</dnaseqindica>

External Link(s)

NCBI Gene:Os02g0170300, RefSeq:Os02g0170300

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Cite error: Invalid <ref> tag; no text was provided for refs named ref1
  2. ↑ 2.0 2.1 2.2 Cite error: Invalid <ref> tag; no text was provided for refs named ref2
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