Difference between revisions of "Os02g0141300"
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===Expression=== | ===Expression=== | ||
| − | + | Analyzing by RT-PCR, a very weak signal was detectable in meiotic-stage spikelets (stages 7 and 8) and in microspore stage anthers (stages 9 and 10), and no signal was detected in leaf blades, roots, lemmas/paleas, or flowering-stage pistils, nor in tricellular pollen stage anthers (stages 12 and 13), while a prominent signal was detected in anthers at the bicellular pollen stage (stage 11). | |
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| + | By in situ hybridization, no signal was detected in the anther of microspore (stage 10), bicellular pollen (stage 11), and tricellular pollen (stage 13) stages using a digoxigenin (DIG)-labeled CAP1 sense probe as a control. By contrast, the hybridization signals by a DIG-labeled CAP1 antisense probe were present not only in developing pollen, but also in tapetum and endothecium (anther wall). | ||
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| + | Thus, CAP1 is preferentially expressed in anthers during pollen development. The developmental stage, when increased CAP1 expression was observed, coincided with the timing of morphological and biochemical alterations in cap1 mutants, suggesting that CAP1 is closely associated with bicellular-stage pollen at stage 11. | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 11:21, 9 May 2014
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Contents
Annotated Information
The gene CAP1 is a highly conserved plant-specific gene that encode an arabinokinase-like protein which is critical for pollen development in both monocotyledonous and dicotyledonous plants.
Function
The gene Os02g0141300 locates at chromosome 2 and is composed of 28 exons that encode a protein of 996 amino acids. It appears to be a member of the galactokinase, homo-Ser kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) superfamily. The N-terminal half of CAP1 contained a glycosyltransferase family 1 domain (30–338 amino acids), while the C-terminal half contained both a Gal-binding (GB) signature (496–540 amino acids) and a GHMP N-terminal (GHMP-N) domain (638–704 amino acids). The GHMP-N domain is involved in ATP binding (Tsay and Robinson, 1991;Lee and Leustek, 1999).
The protein encoded by CAP1 shows high similarity to Arabidopsis (Arabidopsis thaliana) l-arabinokinase, which catalyzes the conversion of l-arabinose to l-arabinose 1-phosphate. This indicates that CAP1 is a arabinokinase-like protein.
Expression
Analyzing by RT-PCR, a very weak signal was detectable in meiotic-stage spikelets (stages 7 and 8) and in microspore stage anthers (stages 9 and 10), and no signal was detected in leaf blades, roots, lemmas/paleas, or flowering-stage pistils, nor in tricellular pollen stage anthers (stages 12 and 13), while a prominent signal was detected in anthers at the bicellular pollen stage (stage 11).
By in situ hybridization, no signal was detected in the anther of microspore (stage 10), bicellular pollen (stage 11), and tricellular pollen (stage 13) stages using a digoxigenin (DIG)-labeled CAP1 sense probe as a control. By contrast, the hybridization signals by a DIG-labeled CAP1 antisense probe were present not only in developing pollen, but also in tapetum and endothecium (anther wall).
Thus, CAP1 is preferentially expressed in anthers during pollen development. The developmental stage, when increased CAP1 expression was observed, coincided with the timing of morphological and biochemical alterations in cap1 mutants, suggesting that CAP1 is closely associated with bicellular-stage pollen at stage 11.
Evolution
A closely related protein, Os06g0702500, is also found in the rice genome. It encodes a protein with 79% identity to CAP1 and is termed OsARA1.
Similar proteins are found in many higher plants, including Gramineae and Arabidopsis (between 90% and 71% identity), the fern Selaginella moellendorffii (67%), and the moss Physcomitrella patens (63%).
In Gramineae, there are at least two similar proteins in a genome that are divided into two phylogenetically distinct clades, the CAP1 clade (with about 90% identity to CAP1) and the OsARA1 clade (about 80% identity).
The Arabidopsis genome also contains two genes similar to CAP1; AtARA1 (At4g16130) has 75% identity to CAP1 and encods an arabinokinase (Dolezal and Cobbett, 1991; Gy et al., 1998; Sherson et al., 1999), and AtARA2, has 71% identity.
No highly homologous proteins are found in bacteria or animal genomes, which suggests that CAP1is a highly conserved plant-specific gene whose product appears to act as an arabinokinase.
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os02g0141300 |
|---|---|
| Description |
Galactokinase family protein |
| Version |
NM_001052393.1 GI:115444156 GeneID:4328266 |
| Length |
8534 bp |
| Definition |
Oryza sativa Japonica Group Os02g0141300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:2230686..2239219 |
| Sequence Coding Region |
2231383..2231493,2231646..2231849,2231945..2232016,2232293..2232458,2232543..2232758 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:2230686..2239219 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:2230686..2239219 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgaggattcggggagacggcggaggtgatgaggaggaggcggcgatggcggtggtgtcggcgccgccgcagcatctggtcttcgcgtactacatcaccggccatggcttcggccacgccacccgcgcgctcgaggtggtgaggcacctgatcgcggcggggcacgacgtgcacgtggtcaccggcgcgccggagttcgtcttcaccaccgagatcagctcgcccaacctccacatccgcaaggtgctcctcgactgcggcgccgtccaggccgacgccctcaccgtcgatcgcctcgcctccctcgagaagtatcatcagacggccgtgatgccacgcgaatcgattctgaggactgaggtggaatggctcaacacgatcaaggccgacctagtggtttcagatgtcgtccccgtcgcgtgcagggcggctgcggatgctggcattcgatcggtgtgcgtcaccaatttcagctgggacttcatttatgcagagtatgtcgtagtagctggacatcatcatcgctcaattgtgtggcagatagcagaagattattcacattgtgaattcttactacgactccctggatactgccctatgcctgctttccgtgatgtcattgatgttcctcttgtggtgagaagattgcataaatctagatctgaggtgaggaaggaattaggaattaaagatgatgttaaggtggtcattttcaactttggaggacagcctgcaggatggaaactgaagaaagaatggctgcctgatggttggctctgtttggtatgtggtgcatctgaaactcaagagcttccaccaaatttcattaaacttgcaaaggacgcctatacacctgatttgatggcagcatctgactgcatgcttgggaaaattggatatggcactgtgagtgaggctttggcttacaagctgccatttgtatttgttcgtagagattatttcaatgaagagccgtttttgaggaatatgcttgagcattatcaatgtggcgttgagatggtacggagggatttacttactggacactggaaaccttatctgcaacgtgctatgacacttcatccatgctatgatggtccaataaatggtggtgaggtggctgcacatatcctccaggacactgctgttggcaagaagtatatttctggcaagttgagcggagcaagacgtttgcgtgatgccatagtgctaggatatcaactacaaagggctccagggagagatgtcggaattcctgactggtattctgtgtctgagaaagaaatcggtgttcgtccagcaccaacatatcatgaggttaatggaagtgcagagtcatcttttgaggacttcgagatactccatggggacatacaaggattaactgatacaatggcatttttaactagtttatcaggacttgttggaaatgatccaaggagccctgagaagcaatctcgagagagggttgctgcttctgttttctttgattgggaggaggaaatatatgttgcaagagcacctggacgtttagatgtcatgggtggcatcgcagattattcaggaagtcttgtattacagatgcccattcgagaggcttgtcatgttgctattcagagaagtaatcctatgaagcagaagctatggaagcatacacaagctaggcaactagcaaatggcagagcagtgccgttgttacaaattgtatcatttggttctgaattgagtaatcgtgcaccaacctttgatatggacctgtctgattttatggatggtgacaaaccaatatcctatgataaggccaaagaatatttctctcaggacccatcccaaaaatgggctgcatatgttgctggaacaatccttgtgttgatgaccgagctaggtgtggtcttcacagacagcatgagcattctggtttcttcatcagttcctgaaggtaaaggtgtctcctcttctgcatcagtggaggttgccagtatgtctgctattgctgctgcctatggtctaaatatccctccacgggatcttgccatactctgccaaaaggttgagaatcgcattgttggagcaccttgtggtgtaatggatcaaatgacatctgcttgtggagaagctaacaaacttcttgcaatgatttgtcagcctgcagaagtgaaagagttggtcagcattccaactcacattcgattttggggtcttgactctgggatacgacatagtgttggtgggactgattatggctctgtaagggtaggcacttacatgggacgcaagatgatcaagtgtgctgcatctgatcttctttcagaatcattaccctcctgtcctcctattcaatcaggcaacacaaattctgatgaatacgaagaacatggtgtagatcttttgaaatctgaagcgtcgctggagtatttatgcaacctaccacctcatagatatgaagctgtttacgcgagagatattccagagatcataaccggggatgcatttttggagaagtatggagatcataatgatgcagtaacaactgttgacccaaaacgatcttactgtgtgaaggctcctactagacatcctatatacgagaacttccgagttgaggccttcaaagcattgctaacagcagccaaaacagttgagcaactttcggcccttggagaattgatgtatcagtgccactacagctacaatgcttgtgggcttggttctgatggaaccgacaggcttgttaatatggtacaagaagtccagcacaggaagacctcacaggatggtggtcctagtttatttggtgcaaagataactggtggaggatctgggggctcggtttgtgtcattgggaaaaattgcttgaaaagcagtgaagagatttttgagattcagaagagatacaaagctgctaccgggtacctaccaattgttttcgagggctcatctccaggtgcaggcaagtttggatacctgaagattcgacggcgatccacatag</cdnaseq> |
| Protein Sequence |
<aaseq>MRIRGDGGGDEEEAAMAVVSAPPQHLVFAYYITGHGFGHATRAL EVVRHLIAAGHDVHVVTGAPEFVFTTEISSPNLHIRKVLLDCGAVQADALTVDRLASL EKYHQTAVMPRESILRTEVEWLNTIKADLVVSDVVPVACRAAADAGIRSVCVTNFSWD FIYAEYVVVAGHHHRSIVWQIAEDYSHCEFLLRLPGYCPMPAFRDVIDVPLVVRRLHK SRSEVRKELGIKDDVKVVIFNFGGQPAGWKLKKEWLPDGWLCLVCGASETQELPPNFI KLAKDAYTPDLMAASDCMLGKIGYGTVSEALAYKLPFVFVRRDYFNEEPFLRNMLEHY QCGVEMVRRDLLTGHWKPYLQRAMTLHPCYDGPINGGEVAAHILQDTAVGKKYISGKL SGARRLRDAIVLGYQLQRAPGRDVGIPDWYSVSEKEIGVRPAPTYHEVNGSAESSFED FEILHGDIQGLTDTMAFLTSLSGLVGNDPRSPEKQSRERVAASVFFDWEEEIYVARAP GRLDVMGGIADYSGSLVLQMPIREACHVAIQRSNPMKQKLWKHTQARQLANGRAVPLL QIVSFGSELSNRAPTFDMDLSDFMDGDKPISYDKAKEYFSQDPSQKWAAYVAGTILVL MTELGVVFTDSMSILVSSSVPEGKGVSSSASVEVASMSAIAAAYGLNIPPRDLAILCQ KVENRIVGAPCGVMDQMTSACGEANKLLAMICQPAEVKELVSIPTHIRFWGLDSGIRH SVGGTDYGSVRVGTYMGRKMIKCAASDLLSESLPSCPPIQSGNTNSDEYEEHGVDLLK SEASLEYLCNLPPHRYEAVYARDIPEIITGDAFLEKYGDHNDAVTTVDPKRSYCVKAP TRHPIYENFRVEAFKALLTAAKTVEQLSALGELMYQCHYSYNACGLGSDGTDRLVNMV QEVQHRKTSQDGGPSLFGAKITGGGSGGSVCVIGKNCLKSSEEIFEIQKRYKAATGYL PIVFEGSSPGAGKFGYLKIRRRST</aaseq> |
| Gene Sequence |
<dnaseqindica>7727..7837#7371..7574#7204..7275#6762..6927#6462..6677#6271..6347#6069..6164#5940..5983#5698..5785#5334..5415#5129..5259#4834..4956#4333..4416#4076..4235#3806..3969#3341..3397#2942..3061#2724..2833#2531..2633#2378..2431#2052..2114#1895..1962#1751..1808#1470..1536#911..987#704..787#392..568#76..210#gtggagtctctgataataagtgtcgatttttgttgttccgggagaaattccgggagatttggggcggcggcggcgatgaggattcggggagacggcggaggtgatgaggaggaggcggcgatggcggtggtgtcggcgccgccgcagcatctggtcttcgcgtactacatcaccggccatggcttcggccacgccacccgcgcgctcgaggtctcgtcttctcttttttctcgttttggggaggattgattcgtgtttgtctccgtgcgatgaaattttggtttctgcgcggaattttggtttcgtggctggatggatggattcgctgctgtttgagtttttttttaatttttatttggggtgatggaattttggtttgtggttagtgcaggtggtgaggcacctgatcgcggcggggcacgacgtgcacgtggtcaccggcgcgccggagttcgtcttcaccaccgagatcagctcgcccaacctccacatccgcaaggtgctcctcgactgcggcgccgtccaggccgacgccctcaccgtcgatcgcctcgcctccctcgagaaggttccagaaacatactccaatatctccactctactacctgctactgctacaacatccaccactcttgctcttgctgtgctgtgctttgcctgtggatttgtggctgattgagtgattgtgtggtgcggaatgcagtatcatcagacggccgtgatgccacgcgaatcgattctgaggactgaggtggaatggctcaacacgatcaaggccgacctagtggtaatgctcctgaggatgctaacaccaactacttgctcgatttttttaatctgtttttatccggggtctccatgaatctttacagcatctgaagcaaatgtggtgttgttggtctttggtcaggtttcagatgtcgtccccgtcgcgtgcagggcggctgcggatgctggcattcgatcggtgtgcgtcaccaatttcaggttagtgtttctgtggttgcttcatgctaggggtcaaaaggagcaatgaggatgtagagtgagctttagaattattagttacagcctttgtgggttcccatattattccaagtttatgttaacattttgcttggggttatatgtttcatcagtgcatagttgctacagttacctgacttttatatgttttgcctgctaacattcatttcctgatgtaatcagtactaacgctgaacacaaaagttatctactaacccacaagaaatctgcagctctgagtgatgctaacaataaattttagttatggctttacccgaaattgactagttgtaaacataattttttctctccatttatttttataattcgggctattgaagaaatttacatggatagttttatttgcagtttttcttgcttcttttttgagtagttatttttgaatcaaagcacggtctttaattttcaccttctatttgcagctgggacttcatttatgcagagtatgtcgtagtagctggacatcatcatcgctcaattgtgtggcaggtgcctgtcatttcatttcccaaaaatggtgcaaaattgtctaattggtttgctaatgctaactactaaattcctaaagccacctagacaccggattcaaatttcttgatttttttttgagaaaactgttctctgtaatatcgttctttagttgcaagttgagactaattcagcccgttcaataaccatgcatttatgttggcatcaactgcagatagcagaagattattcacattgtgaattcttactacgactccctggatactgccctagtacgtctatatgtctgaatgaataatatatccaaagatcctgcatgttggtttgaagtactcgtgattaattctatcttctgcagtgcctgctttccgtgatgtcattgatgttcctcttgtggtgagaagattgcataaatctagatctgaggtgcgtacaactctgctggacaatgtttgctctgaacactcaacccatgttctcagttcttgatgacttaaaaaatctccatatatcaggtgaggaaggaattaggaattaaagatgatgttaaggtggtcattttcaactttggaggacaggtaaaccattaaatagcctgaatgttctttctcgtttatagatgctcgattttgttcaattaatggatgttagttgcatatttctgttaaatcatttttttttgaaagggctatatttatgttaaatcaatatgtccttttagaccagctaaggtttctgtctttctgctgttattggtagtcagtacaatccatagttgaatttgctaccatcttatgcaagtattgctgatgcaatgccattgtgatggtttcctcttcagcctgcaggatggaaactgaagaaagaatggctgcctgatggttggctctgtttggtatattttctttgaaactacgtgttttcttctctcttctttctctctctcttaataatgtctggacaacatttgtgaccaaattctattttcataaaggtatgtggtgcatctgaaactcaagagcttccaccaaatttcattaaacttgcaaaggacgcctatacacctgatttgatggcagcatctgactgcatgcttggtaaatacagtgctatattatctgtgttagggctatttaccgccttatttcttttctagaaatgtaacaattctgttggttgttgcatagggaaaattggatatggcactgtgagtgaggctttggcttacaagctgccatttgtatttgttcgtagagattatttcaatgaagagccgtttttgaggaatatgcttgaggttgaaacatctcgaagtatagtttcattttaagaattcgtacttatagagcatcagtcatgaaccttgatgtcctgttctatatcctcttttcttcctaataaacagcattatcaatgtggcgttgagatggtacggagggatttacttactggacactggaaaccttatctgcaacgtgctatgacacttcatccatgctatgatggtccaataaatggtggtgaggtaatgtccttacagttctcagatatgggtctgtgagggttgtttgggaactcaagctagtaaccatcttgtaaattttattttttttttatgatggctgatttttaaaactgaaataccatactagccataggttgaagttcatcctgaatcaattcttccgtaagtcatgaagagtcaactggatacttgcatcatgttttcctcaaaccagatgtgattcacagactttcttgattgcatttataatacaagataagctaataattattcgttcaggtggctgcacatatcctccaggacactgctgttggcaagaagtatatttctggcaaggtgagttactaacataaattgttgatacattattgttcagttgtaccttgaaactttcagataaaagtgagactttgatatcttcagttatctgtatttggtgtcgtatggaacttttgttgctttcatactacacatataaagtgataagttgaacataagtaaacacttctgccttttcttgtcatgttactcattaaaagcatttcacccctggaggtttttattaattcttttgctcaagaatattttttttatggaactgtccacctagttgctaattcccaattcagaacagaggatttatctactgctctggaacttgaaaacctatgaagttttaaagttacaagagttttttttttcacaacttctggacttagtatttgcactactctacaattacagttgagcggagcaagacgtttgcgtgatgccatagtgctaggatatcaactacaaagggctccagggagagatgtcggaattcctgactggtattctgtgtctgagaaagaaatcggtgttcgtccagcaccaacatatcatgaggttaatggaagtgcagagtcgtaagtttccatatcctttcttttcccctcataataacatgtactttatccaataacacactttatcatatccccgtccattcaaataattttgttttggtgtcagatcttttgaggacttcgagatactccatggggacatacaaggattaactgatacaatggcatttttaactagtttatcaggacttgttggaaatgatccaaggagccctgagaagcaatctcgagagagggttgctgcttctgttttctttgattgggaggtactatacaagcataaaaataatacactgatttttttgggaaactattgatatgttataggggaaaatcattttcgaatgatttattctgttgtaggaggaaatatatgttgcaagagcacctggacgtttagatgtcatgggtggcatcgcagattattcaggaagtcttgtattacaggtagtttcaaatgcaagagaaagtggcatttcttatagatgagccttcctgtaattccactatgtgataaaactttcacacaaatgatttcaaattttcaatatttaaagattcttcaattctgcatttgactatcattttagtttgtttctgtaactttccttgcatctcctcttgataccattgaaggtaggggttcctcctgcatgtattgtggaatgatatattgacatatacctggaaaactctgaatacatgcatgttctatagaccttcctacaattatttttagatagatgaataaatattatgtctccttgtaagttactaagttattgattgatttgatatatttaaaacataaagaaagtttcttttggcatatgcgattgacattgagatggtccaaattaccagatgcccattcgagaggcttgtcatgttgctattcagagaagtaatcctatgaagcagaagctatggaagcatacacaagctaggcaactagcaaatggcagagcagtgccgttgttacaaattgtatgttatttatcactaactctctaagcatcttcgcttaccttttcttatccttattatgcgttcttaaaacttaattcatttgacaaccaaagatgtagcatatatttagttggatgcttcacagctcatttggacgactgggatcttcttactaaatatgtctgtacaggtatcatttggttctgaattgagtaatcgtgcaccaacctttgatatggacctgtctgattttatggatggtgacaaaccaatatcctatgataaggccaaagaatatttctctcaggacccatcccaaaagtaagaaagatcgtttgttttactctataaaaatcataacttgtcatgcatattcattcttggttctggttcagatgggctgcatatgttgctggaacaatccttgtgttgatgaccgagctaggtgtggtcttcacagacagcatgagcattctggtaagctttgatcctttctgttttcaatcagaatgcagtaatatatgtaaaattccacctctttgtccagtttgtcacctttctcatattggggagataaagagatattcagaactcaaaaggaatattgtttattagtcacaaaaagaaaacagcatctttcacactttctccttccacattctaatcaatcaagtgtttccttccacattctaatcaatcaagtgttctaactcgaatgtttgtattggtcattcatgataattttatttgaatcctcaggtttcttcatcagttcctgaaggtaaaggtgtctcctcttctgcatcagtggaggttgccagtatgtctgctattgctgctgcctatggtaaacgattgacttgaacatctttgttgtaataatgccactattttctttcttttggttggatatctagttctgcagcagccaccactgttgcacatttaacttaactgggcgtgctagttgacatcaattcatgttcttgtcttggcttcaggtctaaatatccctccacgggatcttgccatactctgccaaaaggtaaagtactaataaatgaagggatatagcttcagaatgtggtaggtcttctattgcagagagtcattctttggtgactatgcaggttgagaatcgcattgttggagcaccttgtggtgtaatggatcaaatgacatctgcttgtggagaagctaacaaacttcttgcaatgatttgtcaggttagtaagggtacccagctcttggaggaagtagctaccagacccagtcattcctcagtccaactttgcatttcctctataattaattgatcaaattttgctgcagcctgcagaagtgaaagagttggtcagcattccaactcacattcgattttggggtcttgactctgggatacgacataggtaaatactttccatattgatatcttttgcaagattggttttattgttgggatgcatggtttggttttattgttgggatgcatagttaaccataatcttctttggttcatgcagtgttggtgggactgattatggctctgtaagggtaggcacttacatgggacgcaagatgatcaagtgtgctgcatctgatcttctttcagaatcattaccctcctgtcctcctattcaatcaggcaacacaaattctgatgaatacgaagaacatggtgtagatcttttgaaatctgaagcgtcgctggagtatttatgcaacctaccacctcataggtctcattaccttgatattgacagcaaattttatatggtccttcatagtgtatgccaggttttatgactttggcaaaatttcagatatgaagctgtttacgcgagagatattccagagatcataaccggggatgcatttttggagaagtatggagatcataatgatgcagtaacaactgttgacccaaaacgatcttactgtgtgaaggctcctactagacatcctatatacgagaacttccgagttgaggtttgatatcataaaaccagaatatctacacctctcgattcataatggctgttgatgagatccctgcatgttgttgtctgatgttctactgcttttgctgctttatcctcgttgtttgtatttttaaactttaaaataattctttgagatgtttgcaaatcagaaaactatcttgagagcgcatggttttcagaaccagctttattttttctccataaagaatagcttattcctttcactgatatttttgacacttgttatgcttctcacgtgtaggccttcaaagcattgctaacagcagccaaaacagttgagcaactttcggcccttggagaattgatgtatcaggtatctgtattacaaatgaaagcccagcactgtacatgttgattcacaaaattagcagtcagtggcactgcattgacccttttgttttctggcagtgccactacagctacaatgcttgtgggcttggttctgatggaaccgacaggcttgttaatatggtacaagaagtccagcacaggaagacctcacaggatggtggtcctagtttatttggtgcaaagataactggtggaggatctgggggctcggtttgtgtcattgggaaaaattgcttgaaaagcagtgaagagatttttgaggtattttcgacatcgttttttattgtcagttgtacacaatggatgaccataactacaaagatccaagataaaccctccaagagagttgtgattcaccataatgtttgcattttcatggtattgcatgctaacaaaatatcatttgttttcagattcagaagagatacaaagctgctaccgggtacctaccaattgttttcgagggctcatctccaggtgcaggcaagtttggatacctgaagattcgacggcgatccacatagccatcaatcagcctaactgaagatttggctgtattttaggtccacacaaacatttgtcaggaacctgaatattacctgttcatatggaacacatatcacatcatcccattatgtagtagtaataagctctctagttcatgcaataattctagtgattagtgcacctctggtgcagacttccattgtgtaagtgcagtatccagataataaaatggaagtctttagattgatgttctgcaatagctgatagcctcaacaccatccatttgcatgggtacacagtggctaccgacttcaactattggatcggaggctatgcatgcatcaaggagcagttgcatttgcaagggacaagacatggcttctgatcaagcagacagcagatgcaaactgggcattgtagagtagcagcatggccttctgttttcagatggcaagttgtgctaacctggagagatctgaatgttcagagagtaacacatccttttctccttttttggtagtatcaagcagctggatgatgatttcattcagccttatcatgaatagggcttagttagtgtattatctggaacaatcatttagcagaataattgatcagttagcttagctctagcttcttgttagagttttgttactgtttgcagaagttgatgccagaaagttgtctagaatcagagttatactgttgcctgac</dnaseqindica> 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