Difference between revisions of "BGIOSGA000325"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | This gene was cloned from the root of Shanyou 63, the famous hybrid rice variety (Oryza sativa ssp. indica). It belongs to cohesin-like family, which mediates rice sister chromatid cohesion during mitosis and meiosis. The complete ORF of OsRad21-i, containing the restriction sites of BamHI and SalI introduced by primers, was named Eqfr and cloned into pGEM-T vector. The recombinant clone, DH5α [pGEM-T-Eqfr], was screened and identified using PCR. The plasmids of pGEM-T-Eqfr and pQE30 vector and digested Eqfr were ligated by T4 DNA ligase at 4℃ overnight. The competent cell of M15 [pREP-4] was transformed by the diluted ligation product. The recombinant clones of M15[pQE30-Eqfr, pREP-4] were identified. All of the plasmids in these recombinant clones were respectively extracted and digested with both endonucleases BamHI and SalI. The restriction pattern was in agreement with the predicted pattern. It showed that the target clone containing pQE30-Eqfr was obtained. In addition, the target clones were cultivated and induced to express the desired protein by IPTG. The molecular mass of expressed protein was about 116 000.The expression quantity was high in the first to third hour after induction, but declined after the third hour. This revealed that the ORF of OsRad21-i theoretically deducted at before was right. The expressed protein and system would be beneficial to next step experiment. | |
===Expression=== | ===Expression=== | ||
Revision as of 03:18, 22 May 2014
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Annotated Information
Function
This gene was cloned from the root of Shanyou 63, the famous hybrid rice variety (Oryza sativa ssp. indica). It belongs to cohesin-like family, which mediates rice sister chromatid cohesion during mitosis and meiosis. The complete ORF of OsRad21-i, containing the restriction sites of BamHI and SalI introduced by primers, was named Eqfr and cloned into pGEM-T vector. The recombinant clone, DH5α [pGEM-T-Eqfr], was screened and identified using PCR. The plasmids of pGEM-T-Eqfr and pQE30 vector and digested Eqfr were ligated by T4 DNA ligase at 4℃ overnight. The competent cell of M15 [pREP-4] was transformed by the diluted ligation product. The recombinant clones of M15[pQE30-Eqfr, pREP-4] were identified. All of the plasmids in these recombinant clones were respectively extracted and digested with both endonucleases BamHI and SalI. The restriction pattern was in agreement with the predicted pattern. It showed that the target clone containing pQE30-Eqfr was obtained. In addition, the target clones were cultivated and induced to express the desired protein by IPTG. The molecular mass of expressed protein was about 116 000.The expression quantity was high in the first to third hour after induction, but declined after the third hour. This revealed that the ORF of OsRad21-i theoretically deducted at before was right. The expressed protein and system would be beneficial to next step experiment.
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
BGIOSGA000325 |
|---|---|
| Description |
Os01g0897800 proteinPutative RAD21-3Putative |
| Definition |
Oryza sativa Indica Group BGIOSGA000325, complete gene. |
| LocusTag |
P0506A10.9 |
| Ensembl Transcript ID |
BGIOSGA000325-TA |
| Ensembl Protein ID |
BGIOSGA000325-PA |
| Length |
8833 |
| Source |
Oryza sativa Indica Group ORGANISM Oryza sativa Indica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:42682925..42691757 |
| Sequence Coding Region |
42691634..42691757,42688459..42688717,42688281..42688375,42688133..42688182,42687529..42687578 |
| Genome Context |
<gbrowseImage1> name=IndicaChromosome01:42682925..42691757 source=RiceIndica01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=IndicaChromosome01:42682925..42691757 source=RiceIndica01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>ATGTTCTACTCGCAGTTCATCTTGGCCAAGAAGGGGCCCCTCGGGACCATATGGATCGCGGCGCATTTGGAGCGGAAGCTGCGGAAGAATCAGGTCGCGGATACGGATATCGGCGTCTCCGTAGATTCAATTATATTCCCTGAAGTTCCGATTGCACTTCGATTATCAAGCCATCTTATGCTTGGTGTGGTCAGGATCTATTCTCGCAAGGTGAACTACCTATTCCATGATTGCAGTGAAGCGTTGCTGAAGATAAAACAAGCCTTTAGGTCTACTGCCGTTGATCTTCCCCCTGAAGAATCAACTGCTCCTTATCATTCTATAACACTTCCAGAGACATTCCATCTGGATGATTTTGAATTACCGGAGGCTGCATTTCAGGGTGACATTGATCATCATGTAAGCACAAAAGAACAGATCACCTTGCAAGACAACCCAGAGAAAACTGGATACTCAACATCCCAATTTGGCTTAGATGAAAGATTTGGTGATGGCAATTCTTCACATATTGGGTTGGATTTGGAAGAGGAATTAATGCTGAACAAGGACCAGTCAATTCAACTTGAGGCTGATGATGGCATTATTATTCAAGGTCGATCAGCAGTTCATCCTACCGATATGGAAGTTGATGATAACCAAAATAAAGATGAAAGCGCTGAAGGATACAACATGGAGGATGGACCTTCCAGTCACAACAAACTAAATCCGTTAAGTGCAGATGGTCTAGTAGGAAACAGTCTTCCTAATTGGCATACATACAATGTGCAAACACCTGATCTGAATGATATGCTATTGAACAATGATGGGAATGCAGGACCATCAGCTTCATACTATCAACCTAGTCCCTTCCCTTGTGATGAGCCTGCATCACCAGAGTTTGTAAGTGCCCAGGCCCCGGCTACACCTGGCCTAATGGAAGAGACTGTTCCTTCCAGAGTGCATGAAAGTCCTGTTCTGAGTCCACAGCGAAAAGCTTCACCATCAAGCAATGATGAAACTGCAAAGACAGCTGAAAATGCTAATGATCTTGTGGGTGCTGAGACGACTGAACTTGCATTGACAAAGCCTGTGCAAATTGAATCTTCTGGTGCTGTACAGGAGATTGATTCCTTGAGTCAACAATGTGCAACTGAGGAATTGCCACCCCAAGCTGAAACCTCAAACTTGGAAGCTACCATGGACAAGTCAATGATAAATACTGATGGTGTAGCTGCATCAGGTGAAGCTACAGCTGCTAAAGAAACCACTGAAGTGTCATTGGTTGAAAATTCTTTAGAGCTATGCGCTGATGGTCCTACTGAACCTGTGATTGAGAACCAAACACAGTTTAATGATGGATCAGTTGATGTGCAAGGGGGCGGTCAGCATGCACCTCATGCTATCTTAGCAACTGCAGATTCCCAAGTGAATGTAGAAGCATCAACACAAGAAATGGCATCTAACGATATACCAAGTGACCTACCAACCTTGGAATTTCCAGAACGTGAGAAAATGCTGTCAGCTCCAGACGTTGAATTGTACCAGGCAAATGACTTGGGCCAGGTAACTGCAGAAAAGGGAACCACTGAATCTGATGGAAGTAACAAAGTAGGTAGTCTCACTAGCAGAAAAAGACACTTGGAGGACAGTTTGCCAGCTCTCGAAAGTGCAACTACTGAAAAATTGTCTGGCAGACCACGTGGTAAAAGAACCATTGATGGCATTCCTGATGATGATGATTTACTGGCGTCTATATTAGTTGGTAGAAGGACCCCTGGACTGAGGCTTGATTCAACACCAATACCACCTAAGACGTCATCTTTGAAACGTCCAAGGTTGACGTCAAAGACTACCACACCGAAGAGAAAAGTGCAAATGGATGATGCCATGGTTATACATGCTGATACCATACGGCAGCAGTTGATCAGCACTGAGGATATAAGGCGCATTCGTAAAAAGGCTCCATGTACCCGTTCCGAAATATGGATGATCGAGAAAGGTTCCTTAGAAGATGATATATTCCATGAGCCCATCTTTTCCTGCATGTGCAAGGAGCTAAACGATTTGCAATATCGGACCTATGAGATTGTTGCTCATCCCACCATTCATAATATGGAAATACATGTACGGCTAGACATGTCCCAAACTATGGCAGATGGCAGCAATGATGTTGGCACCTCTGGCGCAAAGGACAGTGGAAATCACCAAGATCATGTAGTGCTACCAGATGGAGCTGAGTCAGATGCGATGCATCCGGAAGCAACTGATGCAGCAGATGCTAGAACTGATTTTGATTCACACATGCCATCTGACAAACAGGTTAACAATGTTGAAGGAGTAACTGAACAATTAACTGATAATGAGAAAGAAACTGCAGTTGTTGAGAAAGCAACCACTAATATGGGTGACAGTGCTCAAGTTGATTCACTTGATAAGGAATATCTTCAAGATGTTCCAGCTGATCTGCAGAGGAGCACAAATACAAATACACCTCTGTTTGTGCTAGATGACATGCCTGGTCCAGATGTGGTTCTGGATAGCTCTGATCCAGTTTCTGCACAAGCAGTGGATGATATGAAAGGGGAGTTGAGTGACATTGTCCATGACAATGTCAATGCATTTGACAATAAGGATATGCCGACTTCTGAGATTACCGTACTGGAATTTACTCAAAACGCTTCTGGTTTTCCTCAGCCAACAGAGGATGAAAATGTTCTGTCCGCAATGGGTGAAAATTCTGGATTGCAAGAAAATCATGTAGGATCTGTTATGGATCTGGACAATATGGGGCATGACTTTTCACTAAAGGAATGCAGTGATTTTGGCAGTGCGATTCAGGGTGTTGACACAGATTTTCTGATGTATGATGATGAGGTGGACTGTGATGAGGCTAATGATGATGAGCCTAATCCTGAGGAATTCCAGTCTCTTGATAACAGTGGATGGTCTTCACGTACTAGGGGTGTTGCAAGATATCTCAAAACTTTATTTGATGAGGAATCTGGTCTGGGAAGAAAGAGTGTGGCCATTGATCATCTGTTAAGTGGAAAGACTAGGAAAGAAGCATCAAGGATGTTTTTTGAGACATTGGTTCTGACAACAAAGGACTTCATAAGTGTGGATCAACCAAACTCCTTCGATTTTGTCAGCGTTAAGCCAGGGCCAAAGCTCCTGAAGTCAGATTTCTAG</cdnaseq> |
| Protein Sequence |
<aaseq>MFYSQFILAKKGPLGTIWIAAHLERKLRKNQVADTDIGVSVDSIIFPEVPIALRLSSHLMLGVVRIYSRKVNYLFHDCSEALLKIKQAFRSTAVDLPPEESTAPYHSITLPETFHLDDFELPEAAFQGDIDHHVSTKEQITLQDNPEKTGYSTSQFGLDERFGDGNSSHIGLDLEEELMLNKDQSIQLEADDGIIIQGRSAVHPTDMEVDDNQNKDESAEGYNMEDGPSSHNKLNPLSADGLVGNSLPNWHTYNVQTPDLNDMLLNNDGNAGPSASYYQPSPFPCDEPASPEFVSAQAPATPGLMEETVPSRVHESPVLSPQRKASPSSNDETAKTAENANDLVGAETTELALTKPVQIESSGAVQEIDSLSQQCATEELPPQAETSNLEATMDKSMINTDGVAASGEATAAKETTEVSLVENSLELCADGPTEPVIENQTQFNDGSVDVQGGGQHAPHAILATADSQVNVEASTQEMASNDIPSDLPTLEFPEREKMLSAPDVELYQANDLGQVTAEKGTTESDGSNKVGSLTSRKRHLEDSLPALESATTEKLSGRPRGKRTIDGIPDDDDLLASILVGRRTPGLRLDSTPIPPKTSSLKRPRLTSKTTTPKRKVQMDDAMVIHADTIRQQLISTEDIRRIRKKAPCTRSEIWMIEKGSLEDDIFHEPIFSCMCKELNDLQYRTYEIVAHPTIHNMEIHVRLDMSQTMADGSNDVGTSGAKDSGNHQDHVVLPDGAESDAMHPEATDAADARTDFDSHMPSDKQVNNVEGVTEQLTDNEKETAVVEKATTNMGDSAQVDSLDKEYLQDVPADLQRSTNTNTPLFVLDDMPGPDVVLDSSDPVSAQAVDDMKGELSDIVHDNVNAFDNKDMPTSEITVLEFTQNASGFPQPTEDENVLSAMGENSGLQENHVGSVMDLDNMGHDFSLKECSDFGSAIQGVDTDFLMYDDEVDCDEANDDEPNPEEFQSLDNSGWSSRTRGVARYLKTLFDEESGLGRKSVAIDHLLSGKTRKEASRMFFETLVLTTKDFISVDQPNSFDFVSVKPGPKLLKSDF</aaseq> |
| Gene Sequence |
<dnaseqindica>1..124#3041..3299#3383..3477#3576..3625#4180..4229#4629..5404#5498..5881#5963..6107#6375..6511#6688..7463#7623..7656#8125..8233#8323..8452#8735..8833#ATGTTCTACTCGCAGTTCATCTTGGCCAAGAAGGGGCCCCTCGGGACCATATGGATCGCGGCGCATTTGGAGCGGAAGCTGCGGAAGAATCAGGTCGCGGATACGGATATCGGCGTCTCCGTAGGTGGGTGATTTTGCTGTTTCACAAGCTTTTTTCTTTTCGGATCTACTACCATTTTTTTTTGTTTCTGTTCGAGCATGTTAGGGCCTCACACGTAGACTGCTAAAGCATTGTCTTTGCTAATGCTCGTTTGTGCTGTAGGAGGGGGAAAACATTTTTTTTCTCGCTTATTTATGGTTATATGTATTCTTACTGCTCATATGTTGCGATTAATTAGTAAAAGATGTTAGCTTTTTTGGGATAGTGTTAGTTCCTAGTTGACCTAGTTGTTGCTGGCAGAGCAACCATATTTTTACAACTATGCTAATGAGCTGTCTCTTCATTTTTGCTTATACGTGCTCGTTTGCTGGATTATCACTGTTGTACTACAGTAATAGGAAAAGAAACCACCTGTATCAATGCAGTCGCTGATTAGCTTCTTACAACTGATAGATTGGTAAAAAGTTTCAACCTTGAGTTGCACTGGTCATTTGGTTGTTAGTATTAATGCTTCATGTGGATTTTGTTATGCCATCACTAGAGTTGTAAAAGCTATATGCTTTTAGATGTCTGACAAATTGCGGATATAGTGTTTTTGTTCTTTCTCGGGGACAAAGAAAACACTAACTCCCTCACTAGTTACAAGCCACTTGTTCACTAATATGATCGCTAGTTGTTTTATAGATTTAATCGTAATAGTTAAGTACAGCTTATTAGATTGAGAATGCTGGTAGCTTGCCTTCAAGCAGAACTACTACTGGACTGGTTTTATCACGGATATAGTGGTAGAGATGAGATCTCTTTTTTTCCTCTGATGTTGCTCTGTTGCTAATGCTATTATATGCAAGTCAAGCCAGTTCAAAATCTGGGTTTACGATGACCAGAATTCAATACTTCTGTGAAGGTCTCAGACAACATAAGTGCACAGGGTCTGTAGAGAAGGAAGCAAAATTGTAGCATAGGATTTGATTGCATGATAGCTGATAATGCATTTGTACACAGTGAAGCTTGTGTTAAGGATTGGTATCTGTGATGCATGTGGTATGTAAGGACTTGGTTACGGAATTCTAGTTGAAGTGTAGCAAACATGAGAAAGAACATCTTTTTTTTTAGTGCCATGATCATGCAATATTGTGAAGTTGGTTAAGGCTCCGTGATATTAGCTGTCAAGTCGTGTCTCTACACAGTGAAGCTTGTGTTAGGAATAGGAATGTGATAATTTGTAAATGAAGCCCAGCGAACAGAAAGTTATGGAATTTAGAAAAGTGTAAAGCTAGTTAAATGTTGTTTTGACCAAAAAAAAAAAACTACACAAGCATCATTCGGACAAATTTCAAGTAATATTGGAGTGTATATGGGGAATGCCATGAGCTATCCATAGATTCCATTCACAACTGGATTGGGGTGTATATGGGAATTTTATTGAAATATGCAAAGAGGTGCGCCCAACTAAACAATACTCCAATTCACTTTCACCATGTACATCACTTTCCTGCCAAATTTAGAAAGATATGAGGATTCCAAATACAGATTAAAATAATGTTCCAAAGGTACCAACTGATCCGTCTCATTATTTTTTAAAGCATACCATATACGGTCAATATAGAGGATGCGATCCCGACAATAAAGATATAGAGAGAGAGGACTCAAGAAAACCTCTAGTAGCTGTGTCCTGTATGCATGTGCAGCAAAATGAGTAAACCAGAAGCTTCTTTGGTTATTTTCCCATTTATTTAAAGGTAAAAGCAGGCTAGAGTGGCAATGGTTTGATGGAGATCAAGGCTGATAACAACATTATTTCCATCTCCTTATACCAAATTTAATACGTGTCTGTTTCATTGCATGCATATTGAAGGGTGACTAGTATACGACTTGAACCATGTAATTTGTAAAATAAAATAAATACGTGCATGTTAACCCACTTCTTACTTAGAAGATCACTCAAACCTACATAAGTGTATGTAGCATGTAAGAGCCATCGAGCAATGTAAGGTTCCATGCCATATATGCATGAGTTATATATTGAAGAAGGAAAAGAAATCATGTTTTGTTCGCATATGAGAAAAATGCAAGCTGTCGTTGTCTTCTCATTTCGTTCTTACCATTTTGGGATATATTTGTTTTCTTTCGTGCAATTACTGTGTAGTTACTTTTATGTTCCTAGCCTCCTTTTTTTCCGTATTGCTTGCATGGGTTTTAGTAATGCCTGTGTCAATGCATGCTATGCCAATTGGTTGCCAGCTATCTTTGGTAGCCTTTAATTTTCTCCAATGGAAAAATGTGATCAATTTACTTTTTTATGAATGTAAATATTCCACTACATCGGAAATCTAGTGGTGCGGTGTACGCGTGAATGCCTTTGGCCTTGTCCAGGCACTACATATCCTTCCAGCTGTCCTACATCATCCTGTAGCTGCAGCTGCAGTCCCACATTGCTCAGTAGATCCACCCCTGGCCACCACATGTCCTTACATCTAATATGTTAATTTCTTCTAAAAGGATAGCATTGATGATAGAAAGCGCTGTCACGCCATCATTGGGCAAGCAAACCAGCTTATAGCAGTTAATTGGTGAAAAACATTCAACTCCATTCCTTTCTCCAAAGATAAAAATAGAAGTACTTAGATGCCGCATGTGTCTGTTATGATTCTAAATAGTACTCCACTATTTAGTAGTATAAATACAGGAAAAGGTTTCCATTATAAGTTTATTATAACATCAACACATGGACTTAAAATCTTAGAGCAGCTGAAGCATGGCATTAACTCTGCCCATGATACAGGGCAAACCACCATGTGCATCACTAAAACACTCACTAGTGCAATTCACAGAATTTTGTGATGGAAATGTTATGTTTTCCTAACAGTGGTCATATATCTGCCATATGTCTTTTTTTTTTTCATTTTGAGCTTAGTATTAATAATCATTTTTTTCCTGCAGATTCAATTATATTCCCTGAAGTTCCGATTGCACTTCGATTATCAAGCCATCTTATGCTTGGTGTGGTCAGGATCTATTCTCGCAAGGTGAACTACCTATTCCATGATTGCAGTGAAGCGTTGCTGAAGATAAAACAAGCCTTTAGGTCTACTGCCGTTGATCTTCCCCCTGAAGAATCAACTGCTCCTTATCATTCTATAACACTTCCAGAGACATTCCATCTGGATGATTTTGAATTACCGGAGGCTGCATTTCAGGGGTAAGTTCATTGGTTTTGTTGTGATAAGTTTGTTATTTAAAATGTGCCCTTCAACTCTCTTAAGATTTTCCACCATGATCCAGTGACATTGATCATCATGTAAGCACAAAAGAACAGATCACCTTGCAAGACAACCCAGAGAAAACTGGATACTCAACATCCCAATTTGGCTTAGATGGTTGGTCCTTCTGTATTCACGCCATCTACTTTTCGTCATCTACTTTTCAAAATTTGAAGCCAGCCATTTAATCATATTAAGCATATCTCTATTTACAGAAAGATTTGGTGATGGCAATTCTTCACATATTGGGTTGGATTTGGAAGAGGTAATCTCAGCTAATATATCTTTTCTCCAAAAATTCATTGAAATGTTTGTGGTCTTCATCCTGCATTATCTTGTATGTGCTGTTTGGATCTTTTTTTATCATCCCAGTCTTCTCCGCATTATCATAGATGTACTTCCAGAGTAAATATTTACTGTCATGCTCCATAACATGGCAGTTCTTCACATATTGGTTTGTTTTTAGGTCATAATGATAGGGAATGCAGATTATGCAGATGAAAACTCATTTTGAGTTGTACAAGAATGTTTAGAGCATGAACATTGAACATAACAATGATTTTACCATGTGCCAATTCCTTGCGGCCATTGTCTATGCCTTGTCTTCTGGTTGTATATTTGGCAGCTTTGGTTCATTACCTGTTAGGAATCAATATTTCTTTTGAGTATAGGGTAACACATGGCTAATAGATGACTGATTAATGGAAACTCTCTATTTCTGGTTGTTGTGTTATTGTGTCCTATCACTTGATAAACTGCAATTCCAAAACAAAACTTTGTATATCTTCAGCAGCTAAATTTTATTTGTTTCCCCCTCAGGAATTAATGCTGAACAAGGACCAGTCAATTCAACTTGAGGCTGATGATGGGTAAAATCACCTCTTCCACTTTTTTTTTATAAAAAATAGCTATTCAAGTCCCTTGGAAATTTTATCTACACGTCTCTGCTCCCATATAAAGGGTCTTTGATCCTATCTAAAGGAGCCTTTGTGGGTTTTCACAGATGAGGTTAAGTTTCTCTAGGCAATTTTATGTTTTCCTATAACTTCTGACCTTATATTGTACACTTGGACTGAGTCTGAATCAACCCAGGGTCTAGTTACTACTTTCTCCTGATTTGAGTCCAGATGGCCATACTGGGGTGTGACACTGATATATTTGCTACACGTGTTGTGATTTTTTTCCATGACATTTGAAAGTGGCAGCATCTTGCATTTGCCTTTTTCTGTATTTTTACTTTATTTACATTGGTTTGTGCCTCTCTTCAGCATTATTATTCAAGGTCGATCAGCAGTTCATCCTACCGATATGGAAGTTGATGATAACCAAAATAAAGATGAAAGCGCTGAAGGATACAACATGGAGGATGGACCTTCCAGTCACAACAAACTAAATCCGTTAAGTGCAGATGGTCTAGTAGGAAACAGTCTTCCTAATTGGCATACATACAATGTGCAAACACCTGATCTGAATGATATGCTATTGAACAATGATGGGAATGCAGGACCATCAGCTTCATACTATCAACCTAGTCCCTTCCCTTGTGATGAGCCTGCATCACCAGAGTTTGTAAGTGCCCAGGCCCCGGCTACACCTGGCCTAATGGAAGAGACTGTTCCTTCCAGAGTGCATGAAAGTCCTGTTCTGAGTCCACAGCGAAAAGCTTCACCATCAAGCAATGATGAAACTGCAAAGACAGCTGAAAATGCTAATGATCTTGTGGGTGCTGAGACGACTGAACTTGCATTGACAAAGCCTGTGCAAATTGAATCTTCTGGTGCTGTACAGGAGATTGATTCCTTGAGTCAACAATGTGCAACTGAGGAATTGCCACCCCAAGCTGAAACCTCAAACTTGGAAGCTACCATGGACAAGTCAATGATAAATACTGATGGTGTAGCTGCATCAGGTGAAGCTACAGCTGCTAAAGAAACCACTGAAGTGTCATTGGTTGAAAATTCTTTAGAGCTATGCGCTGATGGTCCTACTGAACCTGTGATTGAGAACCAAACACAGTTTAATGATGGATCAGTTGATGTGCAAGGTAAATTGACTATTCGTTGATTTTGTTTTTAAAGTCATTGTATTTGATAAAAAAAAGGTGAAATCATCTGCTTTTTGATTATTTGTTTTGCAGGGGGCGGTCAGCATGCACCTCATGCTATCTTAGCAACTGCAGATTCCCAAGTGAATGTAGAAGCATCAACACAAGAAATGGCATCTAACGATATACCAAGTGACCTACCAACCTTGGAATTTCCAGAACGTGAGAAAATGCTGTCAGCTCCAGACGTTGAATTGTACCAGGCAAATGACTTGGGCCAGGTAACTGCAGAAAAGGGAACCACTGAATCTGATGGAAGTAACAAAGTAGGTAGTCTCACTAGCAGAAAAAGACACTTGGAGGACAGTTTGCCAGCTCTCGAAAGTGCAACTACTGAAAAATTGTCTGGCAGACCACGTGGTAAAAGAACCATTGATGGCATTCCTGATGATGATGATTTACTGGCGTCTATATTAGGTATGGATGAACCTCCTCAACAATAATTGTGCCTGGCTTTTGCTGCATTGTCTAACACTAACTGTGTGGACTTGTATTTAGTTGGTAGAAGGACCCCTGGACTGAGGCTTGATTCAACACCAATACCACCTAAGACGTCATCTTTGAAACGTCCAAGGTTGACGTCAAAGACTACCACACCGAAGAGAAAAGTGCAAATGGATGATGCCATGGTTATACATGCTGAGTATGTATACGCCTCATAGCCTTCAAACAAGTTTGTTGAATGGAACTGTGTGACTGTTGTTCTTTAGTGTTATGTCCTTAAATTTCTGCCATCACTGTCTTCTTATGGGAAACAAATAGTATATTTTTTCCGTATATTCTTGTAAATTTTGTGCAGTATATACATTGAGTTGATTGGTCCATCTCTGGAAATCAGATTGCGGACTATTTTGCTGTCCAAATTATTTTCTGCTGTGATATTATTTACTGAGTTTCTTCCATTCTCCAGTACCATACGGCAGCAGTTGATCAGCACTGAGGATATAAGGCGCATTCGTAAAAAGGCTCCATGTACCCGTTCCGAAATATGGATGATCGAGAAAGGTTCCTTAGAAGATGATATATTCCATGAGCCCATCTTTTCCTGTAAGCTACTTAAATTGGTTTTTATTCTCTGCTTCCTCTTTACACATTTACTGTATGTTCAATTCAATTAGGAAAGAACTGAAACAGAAGTGAAATTAGTTTTGATATATGAGGATAATTAGTGGTTAGTTTTATGCTCTGAAATTCTACTGATGCATGTTTGTGTTCCCAACTAGGCATGTGCAAGGAGCTAAACGATTTGCAATATCGGACCTATGAGATTGTTGCTCATCCCACCATTCATAATATGGAAATACATGTACGGCTAGACATGTCCCAAACTATGGCAGATGGCAGCAATGATGTTGGCACCTCTGGCGCAAAGGACAGTGGAAATCACCAAGATCATGTAGTGCTACCAGATGGAGCTGAGTCAGATGCGATGCATCCGGAAGCAACTGATGCAGCAGATGCTAGAACTGATTTTGATTCACACATGCCATCTGACAAACAGGTTAACAATGTTGAAGGAGTAACTGAACAATTAACTGATAATGAGAAAGAAACTGCAGTTGTTGAGAAAGCAACCACTAATATGGGTGACAGTGCTCAAGTTGATTCACTTGATAAGGAATATCTTCAAGATGTTCCAGCTGATCTGCAGAGGAGCACAAATACAAATACACCTCTGTTTGTGCTAGATGACATGCCTGGTCCAGATGTGGTTCTGGATAGCTCTGATCCAGTTTCTGCACAAGCAGTGGATGATATGAAAGGGGAGTTGAGTGACATTGTCCATGACAATGTCAATGCATTTGACAATAAGGATATGCCGACTTCTGAGATTACCGTACTGGAATTTACTCAAAACGCTTCTGGTTTTCCTCAGCCAACAGAGGATGAAAATGTTCTGTCCGCAATGGGTGAAAATTCTGGATTGCAAGAAAATCATGTAGGATCTGTTATGGATCTGGACAATATGGGGCATGACTTTTCACTAAAGGAATGCAGTGTAAGTCCATATAGAAGTTTCCACTTGTCTTCAGACACTTGTCAGATGACTTGTGTTTCTCTTAATTCCACAATGTAGCATGCTATACAGTACTTGCACTATTTTATTCAGTCGTTATTTAAAGTCCATGGCATAATTGGTATGCTGTTCACATTGCAGGATTTTGGCAGTGCGATTCAGGGTGTTGACACAGGTATTGCCTTTTGTTCTGATACATAAGTGTAAACTAGTGTAGTAGTGTTCTAATATAATGTGGATTATGTGTACAATGCTTTGTAGGTGCAGCAGAAAACTAGCTAAATACCCATGCACAGATTTTCTAATAAATATATCATCAGTTCGAAACTCAAAAAGTGAGCATATTTATCCTGAGAATCATATGTAATTTGCAAGACGCATAACCATAAAGAAGTGGCGGTGGGATTTCAGACTCACCATAACCACCATTCCTAGGTTCTTTTGTATTAGTAGAAATAAACATGACATTAACCTTCATGACTAGTGCAATATCCGTTCTTAATTGTATTTTATATGCAAATTTTGTGATGGGAACTTCCACACGCCCGTATGTGACACTGGTGGATCTTACTGTTCTAGTGTTGCATCGCGAGAGGTTTATAAGTTTTATGACTTGTGTCATATTTGTACTCTACACTTTCAGATTTTCTGATGTATGATGATGAGGTGGACTGTGATGAGGCTAATGATGATGAGCCTAATCCTGAGGAATTCCAGTCTCTTGATAACAGTGGATGGTCTTCACGTACTAGGTACGATTGGATTTCGTGATAATATTCTCAGGGTTTAATGCTCAATATTTTTTTGAGTTCTATAATGAGTTGTTACAATTGCTCTTCAGGGGTGTTGCAAGATATCTCAAAACTTTATTTGATGAGGAATCTGGTCTGGGAAGAAAGAGTGTGGCCATTGATCATCTGTTAAGTGGAAAGACTAGGAAAGAAGCATCAAGGATGTTTTTTGAGACATTGGTAAGACGGTTATGCTTTTCTGTTTCTTCACTCAAGAAATGGAATACCTACCTTCAGCTCCAGTGTGATTGGAGGCACCTAGAATTGCATGATCAAGAATACGCCATTCATTTTATATTGCATGTTTCGATTAGCCATCTTCTCATTAGACAATCTTACTGGCATCAACCAATTTCTGTAGTCATTGTCAAACTAAACCTGCAATGGTAGCTTCAATTGTTTTCTCGAATCATTTCTGTTGACATTATCCTTGGTACTTATTGTGTTCTGTTCCATTCACAGGTTCTGACAACAAAGGACTTCATAAGTGTGGATCAACCAAACTCCTTCGATTTTGTCAGCGTTAAGCCAGGGCCAAAGCTCCTGAAGTCAGATTTCTAG</dnaseqindica> 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| External Link(s) |
