Difference between revisions of "Os04g0649100"
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| − | + | The Expression of SHAT1 in AZ Is Positively Regulated bySH4。To exploit the effect ofSHAT1on AZ differentiation, we examinedSHAT1expression during early floral development using in situ hybridization. The inflorescence and spikelet developmental stages used in this study were according to the criteria reported before. Our analyses ofSHAT1gene expression in the wild type showed that when lemma and palea primordia were first visible on the flanks of the floral meristem at sp6 stage,SHAT1mRNA showed hotspots of expression in palea andlemma primordia .Subsequently,SHAT1signal declined in lemma and palea but appeared in the inner floral organ primordia, such as stamens and carpels during stage sp7, when carpel primordia began to differentiate . When the ovule was first visible,SHAT1 transcripts were restricted to the AZ and anthers from early stagesp8. Afterwards, during late sp8 stage, SHAT1 expression accumulated to higher levels in the AZ than during early stage sp8. We also characterized SHAT1 expression patterns in GLA4. Its genetic background is very close to the wild type, as mentioned above, but itssh4-1allele is the cultivated rice type. Therefore, we used GLA4 as the sh4-1 mutant line in this study. TheSHAT1signal in GLA4 was very similar to that in the wild type, showing converging expression in AZ from early stage sp8 and becoming more intense during late stage sp8. By contrast, the sh4-2mutation completely disruptedSHAT1expression in AZ, with no signals observed during stages sp6-sp8, similar to that in theshat1mutant. | |
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===Evolution=== | ===Evolution=== | ||
Revision as of 15:11, 24 May 2014
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Annotated Information
Function
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The Expression of SHAT1 in AZ Is Positively Regulated bySH4。To exploit the effect ofSHAT1on AZ differentiation, we examinedSHAT1expression during early floral development using in situ hybridization. The inflorescence and spikelet developmental stages used in this study were according to the criteria reported before. Our analyses ofSHAT1gene expression in the wild type showed that when lemma and palea primordia were first visible on the flanks of the floral meristem at sp6 stage,SHAT1mRNA showed hotspots of expression in palea andlemma primordia .Subsequently,SHAT1signal declined in lemma and palea but appeared in the inner floral organ primordia, such as stamens and carpels during stage sp7, when carpel primordia began to differentiate . When the ovule was first visible,SHAT1 transcripts were restricted to the AZ and anthers from early stagesp8. Afterwards, during late sp8 stage, SHAT1 expression accumulated to higher levels in the AZ than during early stage sp8. We also characterized SHAT1 expression patterns in GLA4. Its genetic background is very close to the wild type, as mentioned above, but itssh4-1allele is the cultivated rice type. Therefore, we used GLA4 as the sh4-1 mutant line in this study. TheSHAT1signal in GLA4 was very similar to that in the wild type, showing converging expression in AZ from early stage sp8 and becoming more intense during late stage sp8. By contrast, the sh4-2mutation completely disruptedSHAT1expression in AZ, with no signals observed during stages sp6-sp8, similar to that in theshat1mutant.
Evolution
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Structured Information
| Gene Name |
Os04g0649100 |
|---|---|
| Description |
Pathogenesis-related transcriptional factor and ERF domain containing protein |
| Version |
NM_001187194.1 GI:297723518 GeneID:9269072 |
| Length |
2030 bp |
| Definition |
Oryza sativa Japonica Group Os04g0649100, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:33471877..33473906 |
| Sequence Coding Region |
33471878..33472020,33472167..33472296 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:33471877..33473906 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:33471877..33473906 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>tatgtcctaatcgatcaccgtttcttcctctttcgttcgatcgttcgttcttgggcaatctcccgtaaatcatcttgtgggtttttggtgcactgcaggtacgtctacttggggctgttcgacaccgaggaggaagctgccagggcgtacgaccgcgctgccatcaagtgcaatggcaaggatgcggtgacgaacttcgatccaagcatttacgccggggagttcgagccgccgggtgggtgtgtcatgtgtgcgcttactgctcatggctag</cdnaseq> |
| Protein Sequence |
<aaseq>YVLIDHRFFLFRSIVRSWAISRKSSCGFLVHCRYVYLGLFDTEE EAARAYDRAAIKCNGKDAVTNFDPSIYAGEFEPPGGCVMCALTAHG</aaseq> |
| Gene Sequence |
<dnaseqindica>2..144#291..420#gtatgtcctaatcgatcaccgtttcttcctctttcgttcgatcgttcgttcttgggcaatctcccgtaaatcatcttgtgggtttttggtgcactgcaggtacgtctacttggggctgttcgacaccgaggaggaagctgccaggtaaaattattgggccgcactgtgcgcgcgcgcggccattgttgttgaatgtcgagtgtggctgctgcgccgtttgcgagtttacgcttctgggttagatgggcgagcggtgatggtgattgatgtttcttgtctcttggccacagggcgtacgaccgcgctgccatcaagtgcaatggcaaggatgcggtgacgaacttcgatccaagcatttacgccggggagttcgagccgccgggtgggtgtgtcatgtgtgcgcttactgctcatggctaggctgttcgtttgtgagttgtgactgctcatccggacaaaatctgtcactgttttgcagcagcggccacgggtgatgccgccgagcacaacctggacctctcgcttgggagctcggcgggctccaagaggggcaacgttgacggtggcggggacgacgagatcaccggcggcggtggcggtggcgcgggctccgaccagcgcgtccctatggcgttcgaccttgactggcaaacggcggcggcgaggagcaccaaagctaaggtaaaaaaagaatggggactttgctttccgcatcagccgattgtcttctcatcacgtaataaaaaggcttaaaaattcctcgttttcttgtctagttcgaccagaactcgaaccatccccagatgcctccggtcctgcaggtcacccacctgccattcagtcccaggcatcatcaccaagtgggtagctcactatacaactccactccacttcgctccatttgctgctgaatttctcgcgtttggtttcaccggtatccgtgagctgatgaattcttgatgtctgtgctgctagttcttgagcaatggtgatccggggacagcgggaggcctgtcactgacgatcggcgcaggcatggccgggcactggcctccccagcagcagcaggggtggggcaacgccggcggcatgagctggccgcacccgccgcacccgccgccgccgccgaccaacgccgcggccgccgcaaccgctacagcagctgcagcatcatcacgattccctccctacattgcgacgcaagcctcaacctggctgcagaagaacgggttccattccctgacccggcccacctaagatgatcaaagatttaaaattcaagatcgctgcattcatcgtctcctcgtcgatcgatcatggcaaagatcgatggagagagagaggtgtgtgtcactgacgcagggaaaaactcaatgcagatttaccagcaagaacaagagctggatgctattcttttgctctaccttttttcttctctctttcttcttctctgtttcttgattgggcgagcgagatggagatggatctgatcatgaatgggacacaagtgggcatgtaaatgaggagctccctagaaggcgagagagattggccaagcgtcgcctcaagcagtgtaggagtatctatcttttgccgtttcgctcgctttctttccttcttttctttcaccatggccaacaacgatgactctgggtgtcaacatcacagctcattttgttcggtctcatgttgcatcattcactgcatgatgaccatgattcgggaaaatttgctccatgcacctccgcttggccgacaacacttgccctctagtaagcatccttaaacacccacggtttctactactattcatcgctttataaatcctacggagtgctagctttacgctactactactgtcatgtaatctatcatcatcaaaagagacgatcagattaacatatatgtcaactatttaagctttggctctttgctttccaagaaacgtaactgccgagatgtggagcatatagaagtattcgtccttctagatgttggtactaaagtaatgttctttcttttctccctct</dnaseqindica> |
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