Difference between revisions of "Os02g0491300"
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[[File:MTR2.jpg]] | [[File:MTR2.jpg]] | ||
| − | ===Expression=== | + | ===Expression and Location=== |
| − | Please input expression information here. | + | Please input expression and location information here. |
| + | RT-PCR, RNA in situ hybridization and translational fusion between the full-length ''MTR1'' coding region and GFP (Green Fluorescent Protein) under the control | ||
| + | of MTR1’s native promoter (pMTR1:MTR1-GFP) all show the expression of ''MTR1'' only in the anthers(specifically in the microsporocytes and the | ||
| + | tetrads). | ||
| + | The ''MTR1'' transcripts started to be detectable at stage 7 and reached the maximum level at stage 8a, when meiosis occurs to form dyads. At stage 9, when the young microspores are released from the tetrad, the transcript level of MTR1 began to decrease and was hardly detectable at stage 10. | ||
| + | |||
| + | [[File:MTR3.jpg]] | ||
| + | |||
| + | MTR1 was found to localize in the microsporocyte cytoplasm, in the extracellular matrix between the microsporocytes, and at the interface between the microsporocyte and the tapetal cell. And the N-terminal peptide, which is required for MTR1’s localization on the plasma membrane, is critical for MTR1’s role in rice anther development. | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 15:01, 27 May 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Please input function information here. MICROSPORE AND TAPETUM REGULATOR1 (MTR1) is a rice fascilin glycoprotein protein. It may act as an extracellular adhesion molecule in male reproductive development by regulating the cell-to-cell communication between reproductive cells and their adjacent somatic cells.
- an essential gene for programmed tapetal cell fate and pollen formation in rice.
- is required for both the development of the diploid anther wall cells and maturation of the postmeiotic haploid microspores.
- effect the deposition of sporopollenin precursors on the surface.
Mutation and Phenotype
Please input Mutation and Phenotype information here.
mtr1 shows normal vegetative and nonreproductive floral organ development, but has smaller and pale-yellow anthers that fail to produce viable pollen grains.(A: wiletype; B: mtr1)
The result of (DAPI) stain show that start from stage 9 mtr1 microspores show slow development, did not seem to go through mitosis, and were eventually aborted. Transverse section analysis showe mtr1 anther wall layers were less degenerated than wild-type and microspores were collapsed.transmission electron microscopy (TEM) and scanning electron microscopy (SEM) of the anther show mtr1 tapetal cells were highly vacuolated and microspores were irregularly shaped with less deposition of sporopollenin precursors on the surface. And, at stage 7 and 8b, when wild-type tapetal cells showed TUNEL-positive nuclei. However, the mtr1 tapetal cells had no detectable TUNEL signals. Several rice tapetum-expressed genes, which are known to be involved in tapetal function and degeneration, displayed abnormal expression patterns in mtr1 .
Expression and Location
Please input expression and location information here. RT-PCR, RNA in situ hybridization and translational fusion between the full-length MTR1 coding region and GFP (Green Fluorescent Protein) under the control of MTR1’s native promoter (pMTR1:MTR1-GFP) all show the expression of MTR1 only in the anthers(specifically in the microsporocytes and the tetrads). The MTR1 transcripts started to be detectable at stage 7 and reached the maximum level at stage 8a, when meiosis occurs to form dyads. At stage 9, when the young microspores are released from the tetrad, the transcript level of MTR1 began to decrease and was hardly detectable at stage 10.
MTR1 was found to localize in the microsporocyte cytoplasm, in the extracellular matrix between the microsporocytes, and at the interface between the microsporocyte and the tapetal cell. And the N-terminal peptide, which is required for MTR1’s localization on the plasma membrane, is critical for MTR1’s role in rice anther development.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
| Gene Name |
Os02g0491300 |
|---|---|
| Description |
Conserved hypothetical protein |
| Version |
NM_001053407.2 GI:297599244 GeneID:4329387 |
| Length |
2000 bp |
| Definition |
Oryza sativa Japonica Group Os02g0491300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:17929370..17931369 |
| Sequence Coding Region |
17929425..17931176 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:17929370..17931369 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:17929370..17931369 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgctcctcttccgcatctcgctcctcctcctcctcgtcccactcatccccaccgccgccgcgtcccaccaccactcgccggcgggaggaggaggagcggcggtgccgcttcacccgcgccgccaccaccgctccgtcgcgaacacggccacggcgctgttctaccccgccccttccatgcaccagaaccacatcgaggcggaggagggccagttgctgcacgtgctcgccgaccccttcgccgccgcgcccgccgctgccgaggctccgtccggggagaccgccatcgccgcggtgggagcggcggcggaggaggcgacccctacgctgatcgacgactccccgcagcaagcagcggccgcatcaccaccaccaccaccaccacctcctcctcctcctcctcctctcttcgccaagccggatctggactccacggcgccaccgcagccgaaggaggagggcgtggacgggtacgggtcgacgacggcgacggcgacggtgacagcggctccgccactcgacgaacctgctgcagccacggccacgaccacgaccacgaccacgacgacgcttccgctcccgaggtacagccacgtagcgagccctcctccgcctccagtccacgccggggtcgcgggcttgggcgacgagcagcgcctggagcagctggcgagggtgctctcctcgctggggtacaacgagatggcctcggcggcgctgctcctcgccaactcggcgttgctcgcggcgtggccagggtcgatcaccgtcttcgcggctcccgacgtcttcctccgcgcctcttgccccatgtgctcgcgccgccatgtcctactggagcacatcgccctgggctacttcccctacaccgagctcgccgccgcctccaccgccaagctcccctcggcttccccgggcttgtgcctcaacctcgcctccgaccacgggccgttcgccatccaccacgtcaggctctacgtcgacggcgtcgaggtctcgcacccggagctctacaacgacgggcgctacgtcgtgcacggcctccacggcttcctgccgccgctctcccacggctcgtgctcccacggctcgaatcatcgccaccactaccactaccagtaccaccaccaccaccaccacatcatcgccagttcggccgcgtcgtccgccgcgaccgccgcctccgtggtgcgcatcatgatccgggaggccatcgcccgcctccgcgacagcggctacggcttcgtggcgctggccatgcgcgtcaagttcgccgagctcgagaggttggcgaacatgacggtgttcgcgctcgacgaccaggcaatcttcgtcggcggaggccacgactacgtctcggcggtccgcttccacgtcgtccccgggcaccgcctcacccacgccgacctccagcgcctccacccgggcacgatgctccccacgctggccggcgagggccagaacctcgtcgtcacccagggcgcgtccggctccggctccggccccagagacgtgcgcatcaactacatccccatcaaggaccccgacgtggtgatcaactcgcgcatcgccttgcacggcgtctacgtgacgttcccgcgcctccacctcgccaacctcgccgccgcggtcgccctcgcctcctccaaccagatcaacgcaacctgcggcgtcttcggcgactgcgcctccgccgcggcgacctctaccactgttccggctgctcaccgctacggcgaagggcagtga</cdnaseq> |
| Protein Sequence |
<aaseq>MALLFRISLLLLLVPLIPTAAASHHHSPAGGGGAAVPLHPRRHH RSVANTATALFYPAPSMHQNHIEAEEGQLLHVLADPFAAAPAAAEAPSGETAIAAVGA AAEEATPTLIDDSPQQAAAASPPPPPPPPPPPPPLFAKPDLDSTAPPQPKEEGVDGYG STTATATVTAAPPLDEPAAATATTTTTTTTTLPLPRYSHVASPPPPPVHAGVAGLGDE QRLEQLARVLSSLGYNEMASAALLLANSALLAAWPGSITVFAAPDVFLRASCPMCSRR HVLLEHIALGYFPYTELAAASTAKLPSASPGLCLNLASDHGPFAIHHVRLYVDGVEVS HPELYNDGRYVVHGLHGFLPPLSHGSCSHGSNHRHHYHYQYHHHHHHIIASSAASSAA TAASVVRIMIREAIARLRDSGYGFVALAMRVKFAELERLANMTVFALDDQAIFVGGGH DYVSAVRFHVVPGHRLTHADLQRLHPGTMLPTLAGEGQNLVVTQGASGSGSGPRDVRI NYIPIKDPDVVINSRIALHGVYVTFPRLHLANLAAAVALASSNQINATCGVFGDCASA AATSTTVPAAHRYGEGQ</aaseq> |
| Gene Sequence |
<dnaseqindica>56..1807#gtcttcctctccccaagccattcggcaaaagctctctcgagggaagcgcgcatccatggcgctcctcttccgcatctcgctcctcctcctcctcgtcccactcatccccaccgccgccgcgtcccaccaccactcgccggcgggaggaggaggagcggcggtgccgcttcacccgcgccgccaccaccgctccgtcgcgaacacggccacggcgctgttctaccccgccccttccatgcaccagaaccacatcgaggcggaggagggccagttgctgcacgtgctcgccgaccccttcgccgccgcgcccgccgctgccgaggctccgtccggggagaccgccatcgccgcggtgggagcggcggcggaggaggcgacccctacgctgatcgacgactccccgcagcaagcagcggccgcatcaccaccaccaccaccaccacctcctcctcctcctcctcctctcttcgccaagccggatctggactccacggcgccaccgcagccgaaggaggagggcgtggacgggtacgggtcgacgacggcgacggcgacggtgacagcggctccgccactcgacgaacctgctgcagccacggccacgaccacgaccacgaccacgacgacgcttccgctcccgaggtacagccacgtagcgagccctcctccgcctccagtccacgccggggtcgcgggcttgggcgacgagcagcgcctggagcagctggcgagggtgctctcctcgctggggtacaacgagatggcctcggcggcgctgctcctcgccaactcggcgttgctcgcggcgtggccagggtcgatcaccgtcttcgcggctcccgacgtcttcctccgcgcctcttgccccatgtgctcgcgccgccatgtcctactggagcacatcgccctgggctacttcccctacaccgagctcgccgccgcctccaccgccaagctcccctcggcttccccgggcttgtgcctcaacctcgcctccgaccacgggccgttcgccatccaccacgtcaggctctacgtcgacggcgtcgaggtctcgcacccggagctctacaacgacgggcgctacgtcgtgcacggcctccacggcttcctgccgccgctctcccacggctcgtgctcccacggctcgaatcatcgccaccactaccactaccagtaccaccaccaccaccaccacatcatcgccagttcggccgcgtcgtccgccgcgaccgccgcctccgtggtgcgcatcatgatccgggaggccatcgcccgcctccgcgacagcggctacggcttcgtggcgctggccatgcgcgtcaagttcgccgagctcgagaggttggcgaacatgacggtgttcgcgctcgacgaccaggcaatcttcgtcggcggaggccacgactacgtctcggcggtccgcttccacgtcgtccccgggcaccgcctcacccacgccgacctccagcgcctccacccgggcacgatgctccccacgctggccggcgagggccagaacctcgtcgtcacccagggcgcgtccggctccggctccggccccagagacgtgcgcatcaactacatccccatcaaggaccccgacgtggtgatcaactcgcgcatcgccttgcacggcgtctacgtgacgttcccgcgcctccacctcgccaacctcgccgccgcggtcgccctcgcctcctccaaccagatcaacgcaacctgcggcgtcttcggcgactgcgcctccgccgcggcgacctctaccactgttccggctgctcaccgctacggcgaagggcagtgatcgcgtgagtgcgtctctttcgtcctctgcttctaactagctaggatttagcacctgccggttttgtctgtctctgattaggatgagtaatgtgctgtgatgacatatcgtgctactgcctgtacggttctcggttcatgccatggatcagcaactgcttgaaatgaatgagatcttgaaagaattttcagcg</dnaseqindica> |
| External Link(s) |
