Difference between revisions of "Os12g0597000"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | Many developmental and environmental signals are transduced through changes in intracellular calcium concentrations, yet only a few calcium-binding proteins have been identified in plants. Calcineurin B-like (CBL) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements. RNA profiling using a rice (Oryza sativa cv Nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains. A putative rice CBL gene responded to gibberellic acid, but not abscisic acid, treatment. The CBL gene family in rice contains at least 10 genes and these have extensive similarity to the CBLs of Arabidopsis (Arabidopsis thaliana). In yeast (Saccharomyces cerevisiae) two-hybrid assays, rice CBLs interact with the kinase partners of Arabidopsis CBLs. Only one rice CBL gene, OsCBL2, is up-regulated by GA in the aleurone layer. | + | Many developmental and environmental signals are transduced through changes in intracellular calcium concentrations, yet only a few calcium-binding proteins have been identified in plants. Calcineurin B-like (CBL) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements. RNA profiling using a rice (Oryza sativa cv Nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains. A putative rice CBL gene responded to gibberellic acid, but not abscisic acid, treatment. The CBL gene family in rice contains at least 10 genes and these have extensive similarity to the CBLs of Arabidopsis (Arabidopsis thaliana). In yeast (Saccharomyces cerevisiae) two-hybrid assays, rice CBLs interact with the kinase partners of Arabidopsis CBLs. Only one rice CBL gene, OsCBL2, is up-regulated by GA in the aleurone layer.<ref name="ref1" /> |
| + | |||
===Expression=== | ===Expression=== | ||
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===Knowledge Extension=== | ===Knowledge Extension=== | ||
| − | + | A homolog with 91% sequence identity to OsCBL2 was cloned from barley (Hordeum | |
| + | vulgare cv Himalaya), and designated HvCBL2. We examined the localization and function of OsCBL2 and HvCBL2 in rice and | ||
| + | barley aleurone because changes in cytosolic calcium have been implicated in the response of the aleurone cell to GA. Green | ||
| + | fluorescent protein translational fusions of OsCBL2 and OsCBL3 were localized to the tonoplast of aleurone cell protein storage | ||
| + | vacuoles and OsCBL4-green fluorescent protein was localized to the plasma membrane. Data from experiments using antisense | ||
| + | expression of OsCBL2 and HvCBL2 are consistent with a role for OsCBL2 in promoting vacuolation of barley aleurone cells | ||
| + | following treatment with GA.<ref name="ref1" /> | ||
===Highlight=== | ===Highlight=== | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
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==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1">A Gibberellin-Regulated Calcineurin B in Rice Localizes to the Tonoplast and Is Implicated in Vacuole Function Yong-sic Hwang, Paul C. Bethke, Yong Hwa Cheong, Hur-Song Chang, Tong Zhu, Russell L. Jones Plant Physiology, 2005, 138(3): 1347-1358</ref> | ||
==Structured Information== | ==Structured Information== | ||
Revision as of 02:48, 29 May 2014
Class B enzyme calcineurin subunit (Calcineurin B-like, CBL) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements.
Contents
Annotated Information
Function
Many developmental and environmental signals are transduced through changes in intracellular calcium concentrations, yet only a few calcium-binding proteins have been identified in plants. Calcineurin B-like (CBL) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements. RNA profiling using a rice (Oryza sativa cv Nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains. A putative rice CBL gene responded to gibberellic acid, but not abscisic acid, treatment. The CBL gene family in rice contains at least 10 genes and these have extensive similarity to the CBLs of Arabidopsis (Arabidopsis thaliana). In yeast (Saccharomyces cerevisiae) two-hybrid assays, rice CBLs interact with the kinase partners of Arabidopsis CBLs. Only one rice CBL gene, OsCBL2, is up-regulated by GA in the aleurone layer.[1]
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Mutation
Knowledge Extension
A homolog with 91% sequence identity to OsCBL2 was cloned from barley (Hordeum vulgare cv Himalaya), and designated HvCBL2. We examined the localization and function of OsCBL2 and HvCBL2 in rice and barley aleurone because changes in cytosolic calcium have been implicated in the response of the aleurone cell to GA. Green fluorescent protein translational fusions of OsCBL2 and OsCBL3 were localized to the tonoplast of aleurone cell protein storage vacuoles and OsCBL4-green fluorescent protein was localized to the plasma membrane. Data from experiments using antisense expression of OsCBL2 and HvCBL2 are consistent with a role for OsCBL2 in promoting vacuolation of barley aleurone cells following treatment with GA.[1]
Highlight
Labs working on this gene
Please input related labs here.
References
<references> [1]
Structured Information
| Gene Name |
Os12g0597000 |
|---|---|
| Description |
Similar to Calcineurin B-like protein 2 (SOS3-like calcium binding protein 1) |
| Version |
NM_001073722.2 GI:297613475 GeneID:4352701 |
| Length |
4999 bp |
| Definition |
Oryza sativa Japonica Group Os12g0597000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 12:25266920..25271918 |
| Sequence Coding Region |
25267406..25267526,25267658..25267740,25267878..25267937,25269012..25269120,25269336..25269388 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008405:25266920..25271918 source=RiceChromosome12 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008405:25266920..25271918 source=RiceChromosome12 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggtgcagtgtctcgacggggtgaggcagctgctggcggtcgtgttcaagtgctgcgacctcgagctgaagcagccgcgggggctcgaggatccccaggtcctcgcgagggagaccgtcttcagcgtgagcgaggtcgaggcgctgtacgagctcttcaagaagataagcagtgctgtgattgatgacgggctgattaacaaggaggagtttcagttggcgctgttcaagaccagcaagaaggagagcctgttcgccgaccgtgtatttgatttgtttgacacaaaacacaatggaattttaggatttgatgaatttgctcgtgcactctcagtatttcatccaagtgctccacttgatgagaagattgacttttcattccagttatatgatctcaagcaacaaggctatattgagagacaagaggttaagcagatggttgttgctacacttgctgagtctggaatgaatctttctgatgaaattatagagagcataatcgataagacatttgaggaggcagacacaaagcatgatggaagaattgataaagaagagtggcgcaatctggttcttcgtcatccctctttgctgaagaacatgactctccagtacctcaaggacatcaccactacatttccaagctttgtcttccattcccaggtcgatgacacctga</cdnaseq> |
| Protein Sequence |
<aaseq>MVQCLDGVRQLLAVVFKCCDLELKQPRGLEDPQVLARETVFSVS EVEALYELFKKISSAVIDDGLINKEEFQLALFKTSKKESLFADRVFDLFDTKHNGILG FDEFARALSVFHPSAPLDEKIDFSFQLYDLKQQGYIERQEVKQMVVATLAESGMNLSD EIIESIIDKTFEEADTKHDGRIDKEEWRNLVLRHPSLLKNMTLQYLKDITTTFPSFVF HSQVDDT</aaseq> |
| Gene Sequence |
<dnaseqindica>487..607#739..821#959..1018#2093..2201#2417..2469#2963..3043#3651..3763#4484..4541#agacgcacgcgcacacgcatacgcatcgcagccagttcatcctcgattcctcccccgaatttccttcgcgatctcaccccgcgtgccctagctagctatcgaatcccgtcacctcggcggcggcggcgagatcggagctccgggttaacctcatccaccgcgaggcgtggaacctctctctctctcgctctcaccacctgaggtgcgcgtggccccgctctgtgatccgattcgatcggtcgcgttggagggagggctcctctattctgcgatggcgattgattgattgattgatctggttggggcttggttgcaggatcgaagatcgaaacccctggcaccggctcgggccgcggaatgggcggccgcggtgagtagctaggtctgcggcggcggagttgttgttgttggtggtggtggtggtggaggaggagttggcggcggagggggcgggggcgttggtggtggtggtggtgggagagtatcatggtgcagtgtctcgacggggtgaggcagctgctggcggtcgtgttcaagtgctgcgacctcgagctgaagcagccgcgggggctcgaggatccccaggtcctcgcgagggagaccgtctgtaagtccgccgcattcgccattggttggggcgccgtactcctccttccttttgttgctgagcctgtctcattgggggaatttgactggtgtgtgtggttgggttggattttgtggccttgtgttgtgcagtcagcgtgagcgaggtcgaggcgctgtacgagctcttcaagaagataagcagtgctgtgattgatgacgggctgattaacaaggttggtgtggagtcctctgttcgcgccacgattttgtatcgtagaattgggattgattgattggttgattgatgaaggcatgttattgtgaacctgtatgcaatcactgactgtgtttgtccgttgaccttgtgcaggaggagtttcagttggcgctgttcaagaccagcaagaaggagagcctgttcgccgaccgtgtaagtctgcattgattgcaaacttgttaattattgtatggtgctgcatgccgttgctctgattcggtatatcgcgctcttctcttttggaagttttgaaaagcgacagatttaggcgcgcctagtcaatgaagcatgagcaattgaacactagagactatttgactatactggacaaaattgaatgcttctaattgcatggaaatagtattagttatctaggattatattggtctaagtttcggatatgctatataccgcatatgctttaatttaggatttttcctttcctgtattgtttcctgccaaacatgtctccaaataacatctgaccgttggttagtcatttccctgccatggcttgtcttccgccctatggcctgcaccatttcattatttcccgctctaatttggccttgtccagaattctctaggcttttccgacatcttatctagtaccttttttaatgttaatcctcatgcatgtttgtttgtaaccaaatcattaattactttatacgtgaaatttcttgaactgttatgctatagcatcagtattacattttacatctcgtgtgtacttgattcgtctgtatcagttacaagtttccttaatcacaatgttcatgtttcgcagatggagtgctgtattatgcataattgggactttgcacatgttttaagtgttttggctattatgttattatgctatcattatgagtgtcttttaccttttgcattgcatctagcacacttctgtatttgagttctgatttgtgagctttgatccgtttgttatccaaatttgagtagtacctttcattggccattcgtactataagcgaatgccttatcgtgtaaatatatttttgtagttaaattccattgaaatcaattggtattgttgtatggctaatactgtccatcacaactgttgtcttgttacatttatcagttgaagctacatgtgaccaaattctttgtagaaaaatagctgtatgatagatgtcagtgcagattttgcctttatctccgtgctgcctcatgttcttaatattctccctgctcttacaggtatttgatttgtttgacacaaaacacaatggaattttaggatttgatgaatttgctcgtgcactctcagtatttcatccaagtgctccacttgatgagaagattgactgtgagttcagacgtactgtagctagatattttactcctcatctatgcagttctaaattatgtattgtcacaataatggcttggagcttatttgcaaaagtatttattgtagacttaaagttgatagtctgacttctatgctgtcctccaggttttctcttgtttacttatcttttctcaacaaaaaattctgaagaagctatgcaatttttgcagtttcattccagttatatgatctcaagcaacaaggctatattgagagacaagaggtattgatgccaaattactcattgcacagtgctccttcaagctagcatgttggcttgtctatatggctcacacacttcgattaattcaacattttcttttattgtttctgatacatgaaatcgtggttttattcatgtaactattctgtacttgatggtctatatttgttgttttgttatggcatactgcagaaagaaatgtcattttttcattaatactgtcagatcactgtcgttgaattttacaagattttaaatcacgtagtagcagcaatagcttaatcatgttgcgtttaggtgagttaggttttgattccaatgaattgcactgggctttgttgctgagagatgatgactatatctgccaatctttaaatagctgatatgccattatcaaagctaaatattttaaatagttttgttgtggatgtcattcttttgggtaggatatttccttttacttcatctatagtttataaaattgatttatcaggttaagcagatggttgttgctacacttgctgagtctggaatgaatctttctgatgaaattatagagagcataatcgataaggtatgttacggtttccatttatattttgttgtgtttggttataccctgtattggatggtatttttttagttggtattttgtggtaatcagtaattctcagcctgcttatataggtcagacttttgtatagcctagccttatcatactacatatatactgtatttactttgcaaatgaaaaactgcatcggtatgtttccttattatataagatgttggtaaaaaaaacattgaacttcttggtgaaacatagtaagaaatgtgccaatttgtaacaataagatgacacgtactaaaagaactataaatgtgtaatattatcatagtattttatgtataaatttactcttcattttcatccaaattatatattaatatattatagacaataatgttcgcacaaattattgtggtttaattagatgtgttctggatggcactatttttaaactgatagaagtaatactgattttgtgctattacttttttttaatagatagatatttagatgacatatcctcaataggcttggcataagatatggtcagtttatcttgtttctttttaattgtaacttccaatgctaaactattttttcttatttgtagacatttgaggaggcagacacaaagcatgatggaagaattgataaagaagagtggcgcaatctggttcttcgtcatccctctttgctgaagaacatgactctccagtacctcaagtaagatatgttttttttttgtttatactctgtatatttttaatagcttggctctttttgttcctgtcttttatttacatctgggcacaattctgaattctctcaacttaatgttggacaccttaagttttcatcttgatagttgtaatggcaatgtttactgctgttcttgaacaaaaatggttggattcggattcccattccaaattttatttgccattgcaatgtcttgcaaaggaaagaatgttacggagagagatgcatgcttgaaatgtgtaaatcaagcatcctctaattaaaattgcgcatgcacatagagtcgcatggaggagtgaggagagcatgttgtatcatctcttgattttaaatagtaattgttttatctaatccataatatgtaagaggaaaagaacaagttcatgattttaaatacatgccttgtcaactcactgcgcatatttttatttctaaaaaaacatgtgttgaactactggatgagttggtcgctccatgtttcagattcaagcaatccacttcacagatacaacagcacttcatatattgacaaaaaataacacactgccttttttgttcttaactaaagaagtattttttttcctaaaaaagattaggtgttcataaaaatgttcaagtctataaaaatgttgtagacacttttgtttttctatagcacaagctaatcttgccacatgtgtgcagggacatcaccactacatttccaagctttgtcttccattcccaggtcgatgacacctgaattcttggatttgtttgaactcaaaccatcagaagaatgctcatgccggttcattaagaattttgatattgggttgttggattgggcagagactccagagctctgtaggatgttgcatgttcgatttgagaagttctcaaatctttaaattgatgtaactttgtttctttcgtttggaagtttagtgtggctggagactttcggggagatgttaaatttctgtctgaatccgtagatcctttattctcttcccctcttttctgtttttctggctagcaatcagaatatcttttctgtttttttggctagcaatcagaatatcggatatagttttgtgaatctttcattttgcgtacaaaataggaaccttcaaaaaaattgatcgtgctggaaaatgttctgttggagtttgcaaattgcaatgatcatgaaatgctctccttttttgagagtttgca</dnaseqindica> 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