Os12g0610200

The OsPIL11 gene is a member of the rice Phytochrome‐interacting factors (PIFs) family, in regulating seedling growth.

Annotated Information

Function

Nakamura et al. (2007) identified six phytochrome‐interacting factor‐like (PIL) homologs in rice, designated as OsPIL11 through OsPIL16. OsPIL11 contains six introns and encodes a predicted protein of 637 amino acids. Alignment using the amino acid sequence of the bHLH DNA domain revealed that OsPIL11 is highly similar to Arabidopsis PIF3 [ 1 ]. OsPIL11 encodes a basic helix‐loop‐helix factor localized in the nucleus. OsPIL11‐OX seedlings exhibit an exaggerated shorter aboveground part and undeveloped root system relative to wild‐type seedling. OsPIL11 represses a set of genes involved in auxin pathways and cell wall organization or biogenesis. Exposure to red light or far‐red light relieved growth retardation and promoted seedling elongation in the OsPIL11‐OX lines. The results suggest that light regulation of OsPIL11 expression is probably involved in photomorphogenesis in rice [ 2 ]. In addition, OsPIL13 and OsPIL11 colocalize with OsPRR1, an ortholog of the Arabidopsis APRR1 gene that controls photoperiodic flowering response through clock function. Overexpression of OsLF might repress expression of OsGI and Hd1 by competing with OsPRR1 in interacting with OsPIL13 and OsPIL11 and thus induce late flowering [ 3 ].

Expression

OsPIF11 gene in leaf, root, stem, old and young ear, ear and embryo widely expressed in organization, and the expression level in the blade is significantly higher than other groups, especially in flag leaf, explain OsPIL11 gene expression is regulated by the development, and closely related to the gene expression and phyB.Semi-quantitative rt-pcr test showed that the gene expression is also affected by photoperiod and biological clock. OsPIF11 gene is widely expressed in leaves, roots, stems, young spike, spike, old embryos and other tissues. The expression level in the leaves is significantly higher than other tissues, especially in the flag leaf. The xpression of OsPIF11 gene is regulated by development and is closely related to phyB.

Evolution

The OsPIL11‐OX lines: To construct the OsPIL11‐overexpression vector, the ORF region of OsPIL11 was amplified by PCR from its full length cDNA clone J033088H. The primer pair used for the amplification and introduction of the restriction sites was 5’‐AAACTAGTATGTCCGACGGCAACGAC‐3’ (SpeI site underlined) and 5’ ‐ATGGTCACCTTATGTTTCAGCCCCATCTC‐3’ (BstEII site underlined). The resulting PCR product was digested with SpeI and BstEII and subcloned into the p1390‐Ubi vector between the maize ubiquitin promoter and the nos terminator. The plasmid was introduced into Agrobacterium tumefaciens strain EHA105 [4] by electroporation. [2]. You can also add sub-section(s) at will.

Labs working on this gene

Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China· Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China

References

[ 1 ] Nakamura Y, Kato Y, Yamashino Y, Murakami M, Mizuno T (2007) Characterization of a set of phytochrome‐interacting‐factor‐like bHLH proteins in Oryza sativa. Biosci Biotech Biochem 71: 1183–1191 [ 2 ] Zhou J, Liu Q, Zhang F, Wang Y, Zhang S, Cheng H, Yan L, Li L,Chen F, Xie X Overexpression of OsPIL11, a phytochromeinteracting factor‐like protein gene, represses etiolated seedling growth in rice. Journal Of Integrative Plant Biology  56: 373-387 [ 3 ] Zhao, Xiao-Ling; Shi, Zhen-Ying; Peng, Ling-Tao (2011) An atypical HLH protein OsLF in rice regulates flowering time and interacts with OsPIL13 and OsPIL11. New Biotechnology   28: 788-797 [ 4 ] Hood E, Gelvin S, Melchers L, Hoekema A (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res 2: 208–218

Gene Name	Os12g0610200
Description	Similar to Phytochrome-interacting factor 3 (Phytochrome-associated protein 3) (Basic helix-loop-helix protein 8) (bHLH8) (AtbHLH008)
Version	NM_001073778.1 GI:115489517 GeneID:4352761
Length	4245 bp
Definition	Oryza sativa Japonica Group Os12g0610200, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 12
Location	Chromosome 12:25995255..25999499
Sequence Coding Region	25996330..25996391,25996490..25997264,25997440..25997505,25997637..25997702,25997789..25998031 ,25998268..25998393
Expression	GEO Profiles:Os12g0610200
Genome Context	<gbrowseImage1> name=NC_008405:25995255..25999499 source=RiceChromosome12 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008405:25995255..25999499 source=RiceChromosome12 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgaaccagttcgtccctgattggaacaccaccagcatgggcgacggctttgcgccattaggcgaagacgacgggctcgtcgagctgctatggtgcaatggccacgtcgtcatgcagagccaggcgccgcggaagccgccgaggccggagaagacgacggcggcggcggcggcggcgatggcggaggatgagtcggcgtcgtggtttcagtacccggtcgacgacgtgcttgagaaggacctgttcaccgagctgttcggcgaaatgacggcggccggcggcggcggcggcgacgtccgcagggcggcgtgcaaggaggagcgcggcgcggtcgccgcgttccagagcaggatgatgccgccgccgtggccggcgagggggaaggcggagttcggtgacgtcgacgacgtgtgcggcgtctcggaggtcgtcatggcgaagatggacggggcggcggcggcggagacggtcggcgagtcatcgatgctgacaatcgggtcgagcatctgcgggagcaaccacgtccagacgccgccggtggggaacgggaaggccggcgccggcaccgccggcgccgccagaagggcgcacgacacggcgacggtggcgtcgtcgtcgatgaggtcgaggtcctgcaccgccaaggccgagccgcgcgacgtcgcagccgccggcgtcggcggcaagcggaagcagcgcggcggcgccgccatggagtccgggagccccagcgaggacgtggagttcgagtccgccgccgcaacgtgctcgccggcgcagaagacgacgacggcgaagcggcggcgcgccgccgaggtgcacaacctctccgagaggaggagaagagataggatcaatgagaagatgaaagcattacaggagctcatacctcactgcaacaaaacggacaaagcatcgatgctggatgaagcgatcgagtatctcaagtcactgcagctccagctacagatgatgtggatgggcggcggaatggcgccgccggcggtgatgttcccggcggccggcgtgcaccagtacatgcagcggatgggcgccgtcgggatgggcccaccacacatggcgtccctgccgaggatgccgccgttcatggcgccgccgcccgccgccgtgcagagctcgccggtggtcagcatggccgacccctacgcccgctgcctcgccgtcgaccacctccagccaccgcctccgatgcattacctgcaggggatgagcttctaccagctcgccgcggccaagaaccttcagcagcagcagaacacggcggaggcgccgccaccgccaccggccggaggcaaccgcgcagccgctgactcctga</cdnaseq>
Protein Sequence	<aaseq>MNQFVPDWNTTSMGDGFAPLGEDDGLVELLWCNGHVVMQSQAPR KPPRPEKTTAAAAAAMAEDESASWFQYPVDDVLEKDLFTELFGEMTAAGGGGGDVRRA ACKEERGAVAAFQSRMMPPPWPARGKAEFGDVDDVCGVSEVVMAKMDGAAAAETVGES SMLTIGSSICGSNHVQTPPVGNGKAGAGTAGAARRAHDTATVASSSMRSRSCTAKAEP RDVAAAGVGGKRKQRGGAAMESGSPSEDVEFESAAATCSPAQKTTTAKRRRAAEVHNL SERRRRDRINEKMKALQELIPHCNKTDKASMLDEAIEYLKSLQLQLQMMWMGGGMAPP AVMFPAAGVHQYMQRMGAVGMGPPHMASLPRMPPFMAPPPAAVQSSPVVSMADPYARC LAVDHLQPPPPMHYLQGMSFYQLAAAKNLQQQQNTAEAPPPPPAGGNRAAADS</aaseq>
Gene Sequence	<dnaseqindica>1076..1137#1236..2010#2186..2251#2383..2448#2535..2777#3014..3139#gctctccggcgagtcctcttcctgccctgccctgccctgccctgcattctttctttctccaccaggggaatccagttcacccccagtgctgcttctgctgctgcttctgcatcatcttgccctgttaaaaagacacagtgcccttgttctttcgcagttgcaactagcatctcctcctctacttgtactcacttcacacctcagctcagctcagctcatctcctgtcatctcagctcaaagagaaagagctgaaggtgtaagctgatcaccaggaagcagaggctttttttcagattacagttatctgaaacaaccaacttcagaatcaatcagcaaaggtagagggagtttcttgtttcttttgtgtactactacatgactatatccatatctcctttttcttctacttctttttcttttgtttggttgtgagtgttgttgctgtgtgtttcaacaagtcaactggatagatagattcatagatatgatagtttttatctgaggttggaggtttcccctctcatccaccacacacattttggcaccccaatcctgaatttactgttcaattctcttctttttattcttcctctgttgattgatcatccatgcatgcatactggtatatagtactagtgctcaagtgctcatccttcctgatggtgaatctcttttgagaaaagaaaagaaaaagatctctctcatcttggctttttttcctgttgatctgagcactgctcttctccaggttgtttcattctgcaggtctacagcttttgtcatcaaagtggtagggtttgtactttgtagtagattgcagtgatggcccttctctgcttttttttcattctctgtttgttcttgttgctcttattctgtttcattctactttttttaaacaaatccatccttagagttcagttttcccttaaaatttttccaaacctacaaattttgttctctgtgaattcatttttgcatgattcttgcagaatctgaaatgatcattggctttgcaggtagaaacaagacagagctgctgtgcttctgtgattaattagggttgttaatgccatgaaccagttcgtccctgattggaacaccaccagcatgggcgacggctttgcgccattagggttcgtgcgattcttggatcgaagcttgagatctttccatcgattggaggcgatcggatgagtgattctttggtttcttgttcttcttgttgttgcagcgaagacgacgggctcgtcgagctgctatggtgcaatggccacgtcgtcatgcagagccaggcgccgcggaagccgccgaggccggagaagacgacggcggcggcggcggcggcgatggcggaggatgagtcggcgtcgtggtttcagtacccggtcgacgacgtgcttgagaaggacctgttcaccgagctgttcggcgaaatgacggcggccggcggcggcggcggcgacgtccgcagggcggcgtgcaaggaggagcgcggcgcggtcgccgcgttccagagcaggatgatgccgccgccgtggccggcgagggggaaggcggagttcggtgacgtcgacgacgtgtgcggcgtctcggaggtcgtcatggcgaagatggacggggcggcggcggcggagacggtcggcgagtcatcgatgctgacaatcgggtcgagcatctgcgggagcaaccacgtccagacgccgccggtggggaacgggaaggccggcgccggcaccgccggcgccgccagaagggcgcacgacacggcgacggtggcgtcgtcgtcgatgaggtcgaggtcctgcaccgccaaggccgagccgcgcgacgtcgcagccgccggcgtcggcggcaagcggaagcagcgcggcggcgccgccatggagtccgggagccccagcgaggacgtggagttcgagtccgccgccgcaacgtgctcgccggcgcagaagacgacgacggcgaagcggcggcgcgccgccgaggtgcacaacctctccgagagggtacgtgtcatgcacgctcacattttgttcttcttgctttgacttttctctcttattattattattattattattattattattattattattattattattattattattattattattattattattattgctgctgctgctaataaaaaatgttaaattgattgtgatgcagaggagaagagataggatcaatgagaagatgaaagcattacaggagctcatacctcactgcaacaaagtaaactactctaaatttaatctgcaagcgccattacataattacaactagcaacattgctatacatctcattaaaatttaaatcgaatttatttgaaaatggtgacctcattcttgtttcatattttcagacggacaaagcatcgatgctggatgaagcgatcgagtatctcaagtcactgcagctccagctacaggtttgtactacgtaaaatgaatgtaactcaatactaaaattgattaacgataatccgacgatgatgatgtgaatggcgtggttcagatgatgtggatgggcggcggaatggcgccgccggcggtgatgttcccggcggccggcgtgcaccagtacatgcagcggatgggcgccgtcgggatgggcccaccacacatggcgtccctgccgaggatgccgccgttcatggcgccgccgcccgccgccgtgcagagctcgccggtggtcagcatggccgacccctacgcccgctgcctcgccgtcgaccacctccagccaccgcctccgatggtaagtaaagcacgtgcctgtacagtcaccatgcatcgatcagtaattcagtagctgccatgaaatgtattttcagtttcgacgcgaatactagggaaggaactaatatcaaataatagaaggggtgagccttcgaatcgagatcgtctagcccaccaccttatagagctagccggaaggccctcgagcgtttctcatattttcagtttcctaagagtttttttttttgtcagcagcattacctgcaggggatgagcttctaccagctcgccgcggccaagaaccttcagcagcagcagaacacggcggaggcgccgccaccgccaccggccggaggcaaccgcgcagccgctgactcctgacgacattctgcacaaaaaatacggtgtgtaagtactatctactccacatttcagtgtcaatgtacatgaaacaaaaatgaaaatcagtgatctgctactagtctactagtagtactagtatcgtaccacaaagaatgcttcaaggcctgaaatttcgacccaaatacccgaaatttccgggttccgatcctcgtacaagtactacatatgtcctaaaatataacaacttttacctatgaatctagccaagtgcatgtctagattcgtagtcaaaagttaatgtattttaggatggatgaattatgaaatttcggaacgaaatacctgagaattcgggggtattgatcctccagtagtactatatgttggaaaagaaaaatcagacagaataaatatatgcattttttcgcttgaaattttatttgaaattgccctgtaaaatgagccagaaaagtctaaggaatttaaagatgaataaaatctgagggtgtgaacacaggtttgctttcagcaaagacaatgtgatctggttagctcaagaacctgtcattgtcatgcactgtaggctagagtttactttgctgatttcactcggctacatgcactgcatccatcggtgtcaaattcacggactagttgatcatttatctcttgtagatccgtggatagctgcatcacagaaactctgaatttctctgttcttaaatgcaactgtttgttcatctctgacttgacttatcactcacatatctgtctcttttttgtccctgaaatattgcagacaactgtagtaagtctgagagcaagggtggcaccagttgattgccgtcaatctgaagatatgtatataaggctctgatgcaattgagttgagaggctggatcatgatggctacctaatcattaaggggaatttaagatcatcccttttactgacgtctaaattcggtaggcagtgtagatcgatcagcggttaagagttaacaagtcgtgaattcttttttgctatgtatgtagtactattttttcccctttggggagacattggttatgtatcagttgttgatgtacctgttcaaatttatgccaaccagagcaaattgtatt</dnaseqindica>
External Link(s)	NCBI Gene:Os12g0610200, RefSeq:Os12g0610200