Difference between revisions of "Os09g02650"
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(→Knowledge extension) |
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| − | The rice Brittle Culm 12 (BC12) gene encodes a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. | + | The rice Brittle Culm 12 (BC12) gene encodes a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. <ref name="ref1"/> |
== Function == | == Function == | ||
| − | This gene encodes a kinesin-4 protein, which is participated in cell-cycle progression, cellulose microfibril deposition and cell wall composition in the monocot plant rice. bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition. Similar to the typical kinesin-4 in animals,BC12 has an nuclear locational signal (NLS) and functions in mitosis. BC12 is present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division. BC12 interacts with CDKA;3 (cyclin-dependent kinase) and probably undergoing phosphorylation for the purpose of regulation. BC12 is essential to exert multiple roles in cell division and cell wall biogenesis in rice. | + | This gene encodes a kinesin-4 protein, which is participated in cell-cycle progression, cellulose microfibril deposition and cell wall composition in the monocot plant rice. bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition. Similar to the typical kinesin-4 in animals,BC12 has an nuclear locational signal (NLS) and functions in mitosis.<ref name="ref2"/><ref name="ref3"/> BC12 is present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division. BC12 interacts with CDKA;3 (cyclin-dependent kinase) and probably undergoing phosphorylation for the purpose of regulation. BC12 is essential to exert multiple roles in cell division and cell wall biogenesis in rice. |
== Mutation == | == Mutation == | ||
bc12: A 26bp deletion in the 4th exon of one open reading frame (ORF), results in a frameshift. which is related with the dwarfism and brittleness phenotype. | bc12: A 26bp deletion in the 4th exon of one open reading frame (ORF), results in a frameshift. which is related with the dwarfism and brittleness phenotype. | ||
| − | + | [[File:phenotype 1.jpg]] | |
== Expression pattern== | == Expression pattern== | ||
BC12 is universally expressed in all organs. with higher expression in panicles and culms. RNA in situ hybridization reveals that BC12 is expressed in young tissues, includeing the initiating adventitious roots, primary root tips, flower primordia and intercalary meristems, all tissues in which cells are undergoing vigorous cell division. | BC12 is universally expressed in all organs. with higher expression in panicles and culms. RNA in situ hybridization reveals that BC12 is expressed in young tissues, includeing the initiating adventitious roots, primary root tips, flower primordia and intercalary meristems, all tissues in which cells are undergoing vigorous cell division. | ||
| − | + | [[File:expression pattern.jpg]] | |
== Structure == | == Structure == | ||
| Line 24: | Line 24: | ||
The sequence of this gene are as following: | The sequence of this gene are as following: | ||
| − | 1 ATGACGATGG AGCACGGCGA GGATTGCTGC GTCAAGGTGG CCGTCCATGT | + | <nowiki>1 ATGACGATGG AGCACGGCGA GGATTGCTGC GTCAAGGTGG CCGTCCATGT CCGCCCGCTC ATCGGCGACG AGAAGCTTCA GGGCTGTAAG GACTGCGTCT |
| − | + | 101 CCGTCGTCTC CGGCAAGCCG CAGGTTCAGA TCGGGAGCCA TTCCTTCACC TTCGACCATG TGTACGGCAG CTCCGGCACG CCGTCGGCGG CCATGTTCGA | |
| − | 101 CCGTCGTCTC CGGCAAGCCG CAGGTTCAGA TCGGGAGCCA TTCCTTCACC | + | 201 GGAGTGCGTG GCGCCGCTCG TCGACGGCCT CTTCCAGGGC TACAACGCCA CCGTGCTCGC ATACGGCCAG ACTGGGTCAG GGAAGACCTA CACCATGGGG |
| − | + | 301 ACGGCCTGCA AGGAGGGCTC ACACATTGGG ATTATCCCGC GTGCCATGGC CACGCTGTTC GACAAGATCG ACAAGCTCAA GAACCAAGTA GAGTTCCAGC | |
| − | 201 GGAGTGCGTG GCGCCGCTCG TCGACGGCCT CTTCCAGGGC TACAACGCCA | + | 401 TGCGCGTTTC GTTTATTGAG ATTCTGAAAG AAGAGGTGAG GGATTTGCTT GATCCTGCTA CTGCTGCTGT TGGCAAACTT GAGAATGGAA ATGGACATGC |
| − | + | 501 AACCAAGTTG TCGGTTCCAG GTAAACCCCC TGTTCAGATC CGGGAGGCGT CGAACGGGGT CATAACGTTA GCAGGATCGA CCGAAGTGCA TGTCACTACC | |
| − | 301 ACGGCCTGCA AGGAGGGCTC ACACATTGGG ATTATCCCGC GTGCCATGGC | + | 601 CAGAAAGAAA TGACGGCATG CCTTGAGCAA GGATCTCTGA GTCGTGCAAC TGGAAGTACC AACATGAACA ACCAATCAAG TCGTTCCCAT GCCATCTTCA |
| − | + | 701 CAATCACATT GGAGCAGATG CGCAAAGCAG ATCCCATCAT GACTTTAGAT GGAATGCCTA TTGAAGAGAT GAATGAAGAC TATCTCTGTG CCAAACTCCA | |
| − | 401 TGCGCGTTTC GTTTATTGAG ATTCTGAAAG AAGAGGTGAG GGATTTGCTT | + | 801 CTTAGTAGAT CTTGCTGGAT CAGAACGTGC TAAAAGAACT GGTTCTGATG GCCTTCGGTT TAAGGAAGGT GTTCATATCA ACAGAGGACT TCTTGCTCTT |
| − | + | 901 GGCAATGTCA TCAGTGCTCT CGGTGATGAG AAAAAGAGGA AAGAAGGCGC TCATGTTCCT TACCGGGACA GCAAACTCAC TCGCCTTCTG CAGGACTCTC | |
| − | 501 AACCAAGTTG TCGGTTCCAG GTAAACCCCC TGTTCAGATC CGGGAGGCGT | + | 1001 TAGGTGGAAA CAGCAAGACT GTAATGATAG CCTGTATTAG TCCAGCAGAT ATCAATGCTG AAGAAACACT GAACACTTTG AAATATGCTA ACCGCGCTCG |
| − | + | 1101 TAACATCCAG AATAAGCCAA TTGTAAATAG AAATCCTGTT GCTGATGAGA TGAAAAGGAT GCGCCAGCAA ATTGAATACT TGCAAGCAGA ACTCGTTTCA | |
| − | 601 CAGAAAGAAA TGACGGCATG CCTTGAGCAA GGATCTCTGA GTCGTGCAAC | + | 1201 GCTCGAGGAG GAGTTGTCTT AGATGATGTT CAGGGTCTCA GGGAAAGGAT CTCAATGCTT GAACAGAAAA ATGAAGACCT TTGCAGGGAA CTGTATGACC |
| − | + | 1301 TTCGCAACCA TGGTTACACT GATCCTTGTG AACCTGAACT GCAAAAAATT GGAACTGGTT ACACTAAAGG TGAAGGGCTC AAAAGAAGCT TGCAAAGTAC | |
| − | 701 CAATCACATT GGAGCAGATG CGCAAAGCAG ATCCCATCAT GACTTTAGAT | + | 1401 AGAACCATTT GATGTCCCCA TGACTGACTC AGTAAGAGCA GGCAGCCCTA AAGATATTGA TGATGAAGTG GCCAAAGAAT GGGAACACAC AATGCTGCAG |
| − | + | 1501 GATAGCATGG GCAAAGAGTT GAATGAATTA AACAGACAAC TGGAGCAAAA GGAGTCTGAG ATGAAAATGT ATGGATCTGA CACTGTTGCA CTTAAACAAC | |
| − | 801 CTTAGTAGAT CTTGCTGGAT CAGAACGTGC TAAAAGAACT GGTTCTGATG | + | 1601 ACTTTGGAAA GAAACTTTTG GAGCTTGAAG AAGAGAAAAG AGCTGTACAG CAAGAAAGGG ACAGATTGTT AGCTGAAGTT GAAAGTCTAA ATGCTGATGG |
| − | + | 1701 ACAAACACAC AAGTTGCGAG ATGCCCAGCT GCAAAAATTA AAAACCCTTG AAGCACAGAT TCTAGACCTC AAGAAAAAGC AGGAGAACCA AGTTCAACTT | |
| − | 901 GGCAATGTCA TCAGTGCTCT CGGTGATGAG AAAAAGAGGA AAGAAGGCGC | + | 1801 CTGAAGGAAA AGCAAAAGAG TGATGAAGCT GCTAAGAAGT TGCAGGAAGA AATTCATTCT ATAAAGGCAC AGAAGGTTCA ACTACAACAT AAAATCAAAC |
| − | + | 1901 AAGAAGCGGA ACAATTCCGG CAATGGAAGG CTACCCGTGA AAAGGAACTC CTGCAGTTGA GGAAAGAGGG GCGGCGAAAT GAGTATGAAC GCCACAAACT | |
| − | 1001 TAGGTGGAAA CAGCAAGACT GTAATGATAG CCTGTATTAG TCCAGCAGAT | + | 2001 TCAAGCACTT AATCAGCGGC AGAAGTTGGT TTTGCAGAGG AAGACTGAAG AAGCTGCGAT GGCTACCAAA AGGCTGAAAG AGTTACTAGA GGCTCGGAAA |
| − | + | 2101 TCATCAGGAC GTGACAACTC AGGCATGAAT GGTACTTCTC CTGGCTCTCA TATGACTGAG AAATCATTGC AAAAATGGCT AGAGCAAGAT TTGGAAGTCA | |
| − | 1101 TAACATCCAG AATAAGCCAA TTGTAAATAG AAATCCTGTT GCTGATGAGA | + | 2201 TGGTGCATGT TCATGAAGTT CGAAACGAAT ATGAAAAGCA AAGTCAATTG CGTGCTGCAC TTGGTGAGGA GCTTGCCATT TTAAAACAAG AAGATGTCAT |
| − | + | 2301 GTCTGGTGCA GCTAGTCCGC CCAGAGGGAA GAATGGAAAC TCTAGGGCAA ATACTTTGTC ACCAAATGCA AGACAAGCTA GGATAGCATC ACTTGAAAGC | |
| − | 1201 GCTCGAGGAG GAGTTGTCTT AGATGATGTT CAGGGTCTCA GGGAAAGGAT | + | 2401 ATGGTGACAA TATCTTCAAA TACTCTCGTT GCTATGGCTT CTCAACTCTC AGAAGCTGAA GAAAGAGAGC GTGCATTCTC TGGGCGTGGT CGGTGGAATC |
| − | + | 2501 AGTTGCGTTC AATGGCAGAG GCAAAGAGTT TACTGCAGTA TATATTTAAC GTTGCTGCAG ATGCAAGATG CCAAGTAAGG GAGAAAGAGA TGGAGATCAA | |
| − | 1301 TTCGCAACCA TGGTTACACT GATCCTTGTG AACCTGAACT GCAAAAAATT | + | 2601 GGAAATGAAG GAGCAAATGA CAGAGCTTGT GACCATCCTT CGACACAGCG AATCACGTAG AAGGGAAACG GAAAAGCAGC TTAAGCAAAG AGAGCAGGCA |
| − | + | 2701 GCTGTAACTG CCACTACATC TCCAGGAAAC GGAAATGGTT CAGTGAAGCA CTCTGCTGAT GACTCCAACA CACCATTGTC ACCAGTTGCG GTGCCTGCAC | |
| − | 1401 AGAACCATTT GATGTCCCCA TGACTGACTC AGTAAGAGCA GGCAGCCCTA | + | 2801 AGAAGCAGCT GAAGTACTCT GCTGGAATTG TAAATAGCCC CAGCAAAGGG GTTCCTGCAT TCAACAAACA ACATCTTAAG ATGGTTCCTA TGGCACAGTT |
| − | + | 2901 GCCTGTTGGC AAGAAGGTTT CAATAGCAGG GCAATCAGGA AAACTTTGGA GATGGAAAAG AAGCCACCAC CAGTGGCTAC TGCAGTTCAA GTGGAAGTGG | |
| − | 1501 GATAGCATGG GCAAAGAGTT GAATGAATTA AACAGACAAC TGGAGCAAAA | + | 3001 CAAAAGCCCT GGAAATTGTC CGAGATGATT CGACACAGTG ACGAAACGAT GACAAGGACT CGACCCAGAC CCCAGCTTCT TCCTCATAGA CCTCAAAGAG |
| − | |||
| − | 1601 ACTTTGGAAA GAAACTTTTG GAGCTTGAAG AAGAGAAAAG AGCTGTACAG | ||
| − | |||
| − | 1701 ACAAACACAC AAGTTGCGAG ATGCCCAGCT GCAAAAATTA AAAACCCTTG | ||
| − | |||
| − | 1801 CTGAAGGAAA AGCAAAAGAG TGATGAAGCT GCTAAGAAGT TGCAGGAAGA | ||
| − | |||
| − | 1901 AAGAAGCGGA ACAATTCCGG CAATGGAAGG CTACCCGTGA AAAGGAACTC | ||
| − | |||
| − | 2001 TCAAGCACTT AATCAGCGGC AGAAGTTGGT TTTGCAGAGG AAGACTGAAG | ||
| − | |||
| − | 2101 TCATCAGGAC GTGACAACTC AGGCATGAAT GGTACTTCTC CTGGCTCTCA | ||
| − | |||
| − | 2201 TGGTGCATGT TCATGAAGTT CGAAACGAAT ATGAAAAGCA AAGTCAATTG | ||
| − | |||
| − | 2301 GTCTGGTGCA GCTAGTCCGC CCAGAGGGAA GAATGGAAAC TCTAGGGCAA | ||
| − | |||
| − | 2401 ATGGTGACAA TATCTTCAAA TACTCTCGTT GCTATGGCTT CTCAACTCTC | ||
| − | |||
| − | 2501 AGTTGCGTTC AATGGCAGAG GCAAAGAGTT TACTGCAGTA TATATTTAAC | ||
| − | |||
| − | 2601 GGAAATGAAG GAGCAAATGA CAGAGCTTGT GACCATCCTT CGACACAGCG | ||
| − | |||
| − | 2701 GCTGTAACTG CCACTACATC TCCAGGAAAC GGAAATGGTT CAGTGAAGCA | ||
| − | |||
| − | 2801 AGAAGCAGCT GAAGTACTCT GCTGGAATTG TAAATAGCCC CAGCAAAGGG | ||
| − | |||
| − | 2901 GCCTGTTGGC AAGAAGGTTT CAATAGCAGG GCAATCAGGA AAACTTTGGA | ||
| − | |||
| − | 3001 CAAAAGCCCT GGAAATTGTC CGAGATGATT CGACACAGTG ACGAAACGAT | ||
| − | |||
3101 TGATGTGA | 3101 TGATGTGA | ||
| + | </nowiki> | ||
== Knowledge extension == | == Knowledge extension == | ||
| − | ''FRA1''(FRAGILE FIBER 1) is the homolog gene of ''BC12'' in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The ''fra1'' mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall. | + | ''FRA1''(FRAGILE FIBER 1) is the homolog gene of ''BC12'' in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The ''fra1'' mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall.<ref name="ref4"/> |
| + | |||
| + | == References == | ||
| + | |||
| + | <references> | ||
| + | <ref name="ref1">Mu Zhang, Baocai zhang, Qian Qian, Yanchun Yu, Rui Li, Junwen Zhang, Xiangling Liu, Dali Zeng, Jiayang Li, Yihua Zhou. Brittle Culm 12, adual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. Plant J. 2010 Jul;63(2):312-28.</ref> | ||
| + | <ref name="ref2">Kwon M, Morales-Mulia S, Brust-Mascher I, Rogers GC, Sharp DJ, Scholey JM. The chromokinesin, KLP3A, drives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility. Mol. Biol. Cell. 2004;15:219–233.</ref> | ||
| + | <ref name="ref3">Mazumdar M, Sundareshan S, Misteli T. Human chromokinesin KIF4A functions in chromosome condensation and segregation. J. Cell Biol. 2004;166:613–620.</ref> | ||
| + | <ref name="ref4">Zhong R, Burk DH, Morrison WH, 3rd, Ye ZH. A kinesin-like protein is essential for oriented deposition of cellulose microfibrils and cell wall strength. Plant Cell. 2002;14:3101–3117.</ref> | ||
Latest revision as of 11:01, 1 June 2014
The rice Brittle Culm 12 (BC12) gene encodes a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. [1]
Contents
Function
This gene encodes a kinesin-4 protein, which is participated in cell-cycle progression, cellulose microfibril deposition and cell wall composition in the monocot plant rice. bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition. Similar to the typical kinesin-4 in animals,BC12 has an nuclear locational signal (NLS) and functions in mitosis.[2][3] BC12 is present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division. BC12 interacts with CDKA;3 (cyclin-dependent kinase) and probably undergoing phosphorylation for the purpose of regulation. BC12 is essential to exert multiple roles in cell division and cell wall biogenesis in rice.
Mutation
bc12: A 26bp deletion in the 4th exon of one open reading frame (ORF), results in a frameshift. which is related with the dwarfism and brittleness phenotype.
Expression pattern
BC12 is universally expressed in all organs. with higher expression in panicles and culms. RNA in situ hybridization reveals that BC12 is expressed in young tissues, includeing the initiating adventitious roots, primary root tips, flower primordia and intercalary meristems, all tissues in which cells are undergoing vigorous cell division.
Structure
The CDS coordinates(5'-3') of this gene is from 1158082 to 1167873. The ORF of the BC12 is 3108 nucleotides long and encodes a kinesin protein of the kinesin-4 subfamily which is 1036 amino acid residues long. The predicted molecular weight is about 116386, and the predicted pI is 9.4858.
BC12 harbors a conserved motor domain at the N-terminus, a neck linker after the motor domain,a long coiled-coil region and the NLS at the C-terminus. The coiled-coil region is responsible for dimerization. BC12 shows microtubule-dependent ATPase activity, and the neck linker plays a critical role for this activity.
Sequence
The sequence of this gene are as following:
1 ATGACGATGG AGCACGGCGA GGATTGCTGC GTCAAGGTGG CCGTCCATGT CCGCCCGCTC ATCGGCGACG AGAAGCTTCA GGGCTGTAAG GACTGCGTCT 101 CCGTCGTCTC CGGCAAGCCG CAGGTTCAGA TCGGGAGCCA TTCCTTCACC TTCGACCATG TGTACGGCAG CTCCGGCACG CCGTCGGCGG CCATGTTCGA 201 GGAGTGCGTG GCGCCGCTCG TCGACGGCCT CTTCCAGGGC TACAACGCCA CCGTGCTCGC ATACGGCCAG ACTGGGTCAG GGAAGACCTA CACCATGGGG 301 ACGGCCTGCA AGGAGGGCTC ACACATTGGG ATTATCCCGC GTGCCATGGC CACGCTGTTC GACAAGATCG ACAAGCTCAA GAACCAAGTA GAGTTCCAGC 401 TGCGCGTTTC GTTTATTGAG ATTCTGAAAG AAGAGGTGAG GGATTTGCTT GATCCTGCTA CTGCTGCTGT TGGCAAACTT GAGAATGGAA ATGGACATGC 501 AACCAAGTTG TCGGTTCCAG GTAAACCCCC TGTTCAGATC CGGGAGGCGT CGAACGGGGT CATAACGTTA GCAGGATCGA CCGAAGTGCA TGTCACTACC 601 CAGAAAGAAA TGACGGCATG CCTTGAGCAA GGATCTCTGA GTCGTGCAAC TGGAAGTACC AACATGAACA ACCAATCAAG TCGTTCCCAT GCCATCTTCA 701 CAATCACATT GGAGCAGATG CGCAAAGCAG ATCCCATCAT GACTTTAGAT GGAATGCCTA TTGAAGAGAT GAATGAAGAC TATCTCTGTG CCAAACTCCA 801 CTTAGTAGAT CTTGCTGGAT CAGAACGTGC TAAAAGAACT GGTTCTGATG GCCTTCGGTT TAAGGAAGGT GTTCATATCA ACAGAGGACT TCTTGCTCTT 901 GGCAATGTCA TCAGTGCTCT CGGTGATGAG AAAAAGAGGA AAGAAGGCGC TCATGTTCCT TACCGGGACA GCAAACTCAC TCGCCTTCTG CAGGACTCTC 1001 TAGGTGGAAA CAGCAAGACT GTAATGATAG CCTGTATTAG TCCAGCAGAT ATCAATGCTG AAGAAACACT GAACACTTTG AAATATGCTA ACCGCGCTCG 1101 TAACATCCAG AATAAGCCAA TTGTAAATAG AAATCCTGTT GCTGATGAGA TGAAAAGGAT GCGCCAGCAA ATTGAATACT TGCAAGCAGA ACTCGTTTCA 1201 GCTCGAGGAG GAGTTGTCTT AGATGATGTT CAGGGTCTCA GGGAAAGGAT CTCAATGCTT GAACAGAAAA ATGAAGACCT TTGCAGGGAA CTGTATGACC 1301 TTCGCAACCA TGGTTACACT GATCCTTGTG AACCTGAACT GCAAAAAATT GGAACTGGTT ACACTAAAGG TGAAGGGCTC AAAAGAAGCT TGCAAAGTAC 1401 AGAACCATTT GATGTCCCCA TGACTGACTC AGTAAGAGCA GGCAGCCCTA AAGATATTGA TGATGAAGTG GCCAAAGAAT GGGAACACAC AATGCTGCAG 1501 GATAGCATGG GCAAAGAGTT GAATGAATTA AACAGACAAC TGGAGCAAAA GGAGTCTGAG ATGAAAATGT ATGGATCTGA CACTGTTGCA CTTAAACAAC 1601 ACTTTGGAAA GAAACTTTTG GAGCTTGAAG AAGAGAAAAG AGCTGTACAG CAAGAAAGGG ACAGATTGTT AGCTGAAGTT GAAAGTCTAA ATGCTGATGG 1701 ACAAACACAC AAGTTGCGAG ATGCCCAGCT GCAAAAATTA AAAACCCTTG AAGCACAGAT TCTAGACCTC AAGAAAAAGC AGGAGAACCA AGTTCAACTT 1801 CTGAAGGAAA AGCAAAAGAG TGATGAAGCT GCTAAGAAGT TGCAGGAAGA AATTCATTCT ATAAAGGCAC AGAAGGTTCA ACTACAACAT AAAATCAAAC 1901 AAGAAGCGGA ACAATTCCGG CAATGGAAGG CTACCCGTGA AAAGGAACTC CTGCAGTTGA GGAAAGAGGG GCGGCGAAAT GAGTATGAAC GCCACAAACT 2001 TCAAGCACTT AATCAGCGGC AGAAGTTGGT TTTGCAGAGG AAGACTGAAG AAGCTGCGAT GGCTACCAAA AGGCTGAAAG AGTTACTAGA GGCTCGGAAA 2101 TCATCAGGAC GTGACAACTC AGGCATGAAT GGTACTTCTC CTGGCTCTCA TATGACTGAG AAATCATTGC AAAAATGGCT AGAGCAAGAT TTGGAAGTCA 2201 TGGTGCATGT TCATGAAGTT CGAAACGAAT ATGAAAAGCA AAGTCAATTG CGTGCTGCAC TTGGTGAGGA GCTTGCCATT TTAAAACAAG AAGATGTCAT 2301 GTCTGGTGCA GCTAGTCCGC CCAGAGGGAA GAATGGAAAC TCTAGGGCAA ATACTTTGTC ACCAAATGCA AGACAAGCTA GGATAGCATC ACTTGAAAGC 2401 ATGGTGACAA TATCTTCAAA TACTCTCGTT GCTATGGCTT CTCAACTCTC AGAAGCTGAA GAAAGAGAGC GTGCATTCTC TGGGCGTGGT CGGTGGAATC 2501 AGTTGCGTTC AATGGCAGAG GCAAAGAGTT TACTGCAGTA TATATTTAAC GTTGCTGCAG ATGCAAGATG CCAAGTAAGG GAGAAAGAGA TGGAGATCAA 2601 GGAAATGAAG GAGCAAATGA CAGAGCTTGT GACCATCCTT CGACACAGCG AATCACGTAG AAGGGAAACG GAAAAGCAGC TTAAGCAAAG AGAGCAGGCA 2701 GCTGTAACTG CCACTACATC TCCAGGAAAC GGAAATGGTT CAGTGAAGCA CTCTGCTGAT GACTCCAACA CACCATTGTC ACCAGTTGCG GTGCCTGCAC 2801 AGAAGCAGCT GAAGTACTCT GCTGGAATTG TAAATAGCCC CAGCAAAGGG GTTCCTGCAT TCAACAAACA ACATCTTAAG ATGGTTCCTA TGGCACAGTT 2901 GCCTGTTGGC AAGAAGGTTT CAATAGCAGG GCAATCAGGA AAACTTTGGA GATGGAAAAG AAGCCACCAC CAGTGGCTAC TGCAGTTCAA GTGGAAGTGG 3001 CAAAAGCCCT GGAAATTGTC CGAGATGATT CGACACAGTG ACGAAACGAT GACAAGGACT CGACCCAGAC CCCAGCTTCT TCCTCATAGA CCTCAAAGAG 3101 TGATGTGA
Knowledge extension
FRA1(FRAGILE FIBER 1) is the homolog gene of BC12 in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The fra1 mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall.[4]
References
[4]- ↑ 1.0 1.1 Mu Zhang, Baocai zhang, Qian Qian, Yanchun Yu, Rui Li, Junwen Zhang, Xiangling Liu, Dali Zeng, Jiayang Li, Yihua Zhou. Brittle Culm 12, adual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. Plant J. 2010 Jul;63(2):312-28.
- ↑ 2.0 2.1 Kwon M, Morales-Mulia S, Brust-Mascher I, Rogers GC, Sharp DJ, Scholey JM. The chromokinesin, KLP3A, drives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility. Mol. Biol. Cell. 2004;15:219–233.
- ↑ 3.0 3.1 Mazumdar M, Sundareshan S, Misteli T. Human chromokinesin KIF4A functions in chromosome condensation and segregation. J. Cell Biol. 2004;166:613–620.
- ↑ 4.0 4.1 Zhong R, Burk DH, Morrison WH, 3rd, Ye ZH. A kinesin-like protein is essential for oriented deposition of cellulose microfibrils and cell wall strength. Plant Cell. 2002;14:3101–3117.
