Difference between revisions of "Os04g0497200"
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===Evolution=== | ===Evolution=== | ||
| − | + | 在突变体中，Osglu3-1突变体的根细胞壁中晶体纤维素含量减少，根细胞长度变短，突变体根的分生组织略微变小。外施葡萄糖能够抑制突变体根细胞壁纤维素含量的降低和短根的表型。OsGLU3在不同组织遍在表达，但是在根尖、侧根和冠状根原基强表达。OsGLU3-GFP融合蛋白在根分生组织和伸长区的质膜和FM4-64标记的细胞器表达。 | |
| − | + | rt突变体根的伸长区的细胞伸长有严重缺陷，而且由于根冠细胞不能顺利剥落，导致根尖表皮细胞和皮层细胞的崩溃。rt突变体根中纤维素含量略有增加而纤维素结晶度明显降低，此外，半纤维素的组分在野生型和突变体根中也有差异，rt根的外植体的延展性明显降低。 | |
| − | |||
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 13:14, 2 June 2014
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Contents
Annotated Information
Function
编码内切-1,4-β左旋葡聚糖酶基因，内切-1,4-β左旋葡聚糖酶是细胞膜结合酶，能影响细胞壁的纤维素合成及根细胞的伸长及分裂，从而调节根的伸长。
Expression
内切-1,4-β左旋葡聚糖酶在水稻中组织特异性表达，在根尖、侧根和冠状根原基强表达，但在其他组织表达量低，如茎、叶和穗。
Evolution
在突变体中，Osglu3-1突变体的根细胞壁中晶体纤维素含量减少，根细胞长度变短，突变体根的分生组织略微变小。外施葡萄糖能够抑制突变体根细胞壁纤维素含量的降低和短根的表型。OsGLU3在不同组织遍在表达，但是在根尖、侧根和冠状根原基强表达。OsGLU3-GFP融合蛋白在根分生组织和伸长区的质膜和FM4-64标记的细胞器表达。 rt突变体根的伸长区的细胞伸长有严重缺陷，而且由于根冠细胞不能顺利剥落，导致根尖表皮细胞和皮层细胞的崩溃。rt突变体根中纤维素含量略有增加而纤维素结晶度明显降低，此外，半纤维素的组分在野生型和突变体根中也有差异，rt根的外植体的延展性明显降低。
Labs working on this gene
Plant Materials
Phosphate Stress
Mapping and Cloning
Complementation Test
Cell Wall Isolation
Cellulose Measurement
Glucose Restoration Experiments
References
[1]Jin-Wei Zhang,Lei Xu.OsGLU3, a Putative Membrane-Bound Endo-1,4-Beta-Glucanase, Is Required for Root Cell Elongation and Division in Rice (Oryza sativa L.)[J].Molecular Plant (2012) 5 (1): 176-186.
Structured Information
| Gene Name |
Os04g0497200 |
|---|---|
| Description |
Glycoside hydrolase, family 9 protein |
| Version |
NM_001059739.2 GI:297602979 GeneID:4336284 |
| Length |
3778 bp |
| Definition |
Oryza sativa Japonica Group Os04g0497200, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:25262593..25266370 |
| Sequence Coding Region |
25264636..25265049 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:25262593..25266370 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:25262593..25266370 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgaatgcagcccttctttctctgacgaacaattttggccatgtctcgttctctgctctgacagccggccggctcccgaagaacaacggcatcaaatggcgcggcaactccggcctgtcggatggctccgatctgacggacgtcaagggcgggctcgtcggcggctactacgacgccggcgacaacatcaagttccatttccccttggccttctccatgacaatgctcagctggtcagtgatcgagtacagcgccaagtacaaggcggtaggggagtatgaccatgtgagggagctcatcaagtggggcaccgactacctgctcctcaccttcaactcctctgcttccaccattgacaaagtctatagccaggtacttgctcaaagtcccaattctgaatatatttcattatga</cdnaseq> |
| Protein Sequence |
<aaseq>MNAALLSLTNNFGHVSFSALTAGRLPKNNGIKWRGNSGLSDGSD LTDVKGGLVGGYYDAGDNIKFHFPLAFSMTMLSWSVIEYSAKYKAVGEYDHVRELIKW GTDYLLLTFNSSASTIDKVYSQVLAQSPNSEYISL</aaseq> |
| Gene Sequence |
<dnaseqindica>1322..1735#aacggcacagtgtcacgcgcgcgcgccagtccgggtgcgccacccaagtcaaccaccgcccgctctctctcccccttcaaaacgcccccacccaaagccaccgcacctcgccaccaccgccccaagaatagcacccgcgtttcatgcgctgatcgaccgatcgacccaccagctaagccacccgcagccgcggggcgagccaagcccaccgccgcagccgcagccacagccatgtactcggcgaaccactggggcgggtcgttcgagatcgccgcggacggcgcggcggaggatgaccacagccgcaacatggacctggaccgcggcgcgctgtcggcgcggcagcaccagctggacgagacgcagcagagctggctgctcggcccgccggaggccaagaagaaggacaagtacgtcgacctcggctgcgtcgtcgtcaagcgcaagctcctctggtgggtcctctggaccctcctcgccgccttcatcctcatcggcctccccgtcatcatcgccaagtccatccccaagaagaagccccacgcccctccccccgaccagtacaccgacgccctccacaaggccctcctcttcttcaacgcccagaaatgtcagtcccccctccatcctgtgatatgatcctctgtttgtgcgttagttaattaactaactaggatggaaacctttgccaaaacaagaatcttttgactttgatcagtagagatctcatcgcatttgccatttgtgttagagcttctgaatctgcagacggatttgctcaaaatgagtagaagtatgttgccttgaatttccttaggtttactgagttttgaacttttgacccgtgatattagctgcatgattatgtgttgatcccgaggagagaggatcgagcaagtacaaatctacaggcgagtagtagcactagttagttttaatcagttcagttgagctgtgcacaactgcaaccccacaaatggaatgttctgtgctactgttcatagtcccacagctttgaagaaagtgacactctgatgtggagagaaatctcccaaaaatccagctgcgctttggcttggagtcttggcaatttggcgtctcacgcacgttcgtgcacatgtaggacagccaattgatccattctttaacgcggcacgaagtactcctctgcaatactagtgtacctgtagatctcgccgcatgatctgcttctggatcttgacagatactctgcacttgtagatcttgagcttacactacagtactactagtcatccgattctgacttccatgctcaaaattcagtttgaaacgtgcactgatgaatgcagcccttctttctctgacgaacaattttggccatgtctcgttctctgctctgacagccggccggctcccgaagaacaacggcatcaaatggcgcggcaactccggcctgtcggatggctccgatctgacggacgtcaagggcgggctcgtcggcggctactacgacgccggcgacaacatcaagttccatttccccttggccttctccatgacaatgctcagctggtcagtgatcgagtacagcgccaagtacaaggcggtaggggagtatgaccatgtgagggagctcatcaagtggggcaccgactacctgctcctcaccttcaactcctctgcttccaccattgacaaagtctatagccaggtacttgctcaaagtcccaattctgaatatatttcattatgaatcttggaagaaatatttgatctgtgaaaattttggtgcattgttactatcttggttcttttctgctgtaatcatagctggaagaccgtagaatttataaatgcagagaagcatactgtttcttactacagcagtaaagtaagtttctgaaataaggtcgtagtgagcaaactggtcagttctgtcaattacagtattacatgccgagcaatctattccatcagtaattgattggcacgcctaacaacctttgactcttatcacatgggctccagtagaataaggttcatcatccaatcatctgcatgtgctgaacaataatataattaaaacttaggagtggtcgatctgtctcgatctctcagaactacattttggcatacaatgaaagatgcagtggtaaaactttgattcacattctcttactctgggttgatccaccaggaaaattatgtaaaaacatttgaagttatcagtattaagtgaaaaagaaaatgatgtcaaatcaagcatactaggtctgaacggtgcatgcatgctcctgcaggtgggcattgcgaagatcaacggcacccagccggacgaccactactgctggaaccggccggaggacatggcgtacccacgccccgtccagacggccggctcggcgccggacctcggcggcgagatggcggcggcgctcgcggcggcctccatcgtgttccgcgacaacgccgcctactccaagaagctcgtgaacggcgcggccgccgtgtacaagttcgcgcgcagcagcggccgccgcaccccgtactcccgcggcaaccagtacatcgagtactactacaactccaccagctactgggacgagtacatgtggagcgcggcgtggatgtactacgccacggggaacaacacctacatcaccttcgccaccgacccgcggctgcccaagaacgccaaggccttctacagcatcctcgacttctccgtcttcagctgggacaacaagctgccgggcgccgagctgctgctgtcgcggctccgcatgttcctcaacccgggttacccctacgaggagtcgctcatcgggtaccacaacaccaccagcatgaacatgtgcacctacttcccgcgcttcggcgccttcaacttcaccaagggtgggctcgcgcagttcaaccacgggaagggccagccgctgcagtacaccgtcgccaactccttcctcgccgcgctctacgccgactacatggagtccgtcaacgtccccggctggtactgcggcccctacttcatgaccgtcgacgatctccgctccttcgccaggtctcaggtacgtgtaatcgacgatcgatcacagattttcacaatgcatgcatcaattggaagttgttttgctgacgacggcgatgtacatgtgcaggtgaactacattcttggggataacccgaagaagatgagctacgtggtggggtacggcaagaagtacccgcggcggctgcaccacaggggggcgtcgacgccgcacaacggcatcaagtactcgtgcaccggcgggtacaagtggagggacaccaagggggccgacccgaacgtgctcgtcggcgccatggtcggcgggccggacaagaacgaccagttcaaggacgcgcgcctcacctacgcgcagaacgagccgacgttggtgggcaacgccggcctcgtggccgccctcgtcgccctcaccaacagcggccgcggcgcgggcgtcaccgccgtcgacaagaacaccatgttctccgccgtgccgcccatgttcccggccacgccgccgccgccgtccaagtggaagccgtgagagagaattcaatccaaccggccggccaagtacatcgccgtgcctagtggttccgaaggtgtttgattagatcaacgaggtgtttcggttcgtttggtcatacagtttttggtgatcacgcaattcttttgtattatacacgttaacttcggcaaaatcgatactgtggatgatggaatgctttatgcatgacctgtagatgaaagatgttttcttcatat</dnaseqindica> |
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