Difference between revisions of "Os09g0494200"
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===Function=== | ===Function=== | ||
The rice Os09g0494200 was reported as ''Brittle Culm15'' (''BC15'') which encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant chitinases, a class of glycosyl hydrolases, participate in various aspects of normal plant growth and development, including cell wall metabolism and disease resistance. ''BC15'' was designated as ''Chitinase-Like Protein1''(''OsCTL1'') and will henceforth be referred to as ''BC15/OsCTL1''. | The rice Os09g0494200 was reported as ''Brittle Culm15'' (''BC15'') which encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant chitinases, a class of glycosyl hydrolases, participate in various aspects of normal plant growth and development, including cell wall metabolism and disease resistance. ''BC15'' was designated as ''Chitinase-Like Protein1''(''OsCTL1'') and will henceforth be referred to as ''BC15/OsCTL1''. | ||
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BC15/OsCTL1 is an N-glycosylated membrane protein. Mutation of BC15/OsCTL1 causes reduced cellulose content and mechanical strength without obvious alterations in plant growth. Biochemical assays demonstrated that BC15/OsCTL1 is a Golgi-localized type II membrane protein that lacks classical chitinase activity. Investigation of the global expression profile of wild-type and ''bc15'' plants, using Illumina RNA sequencing, further suggested a possible mechanism by which BC15/OsCTL1 mediates cellulose biosynthesis and cell wall remodeling. And it is localized in the Golgi apparatus with its C-terminus facing the Golgi lumen. | BC15/OsCTL1 is an N-glycosylated membrane protein. Mutation of BC15/OsCTL1 causes reduced cellulose content and mechanical strength without obvious alterations in plant growth. Biochemical assays demonstrated that BC15/OsCTL1 is a Golgi-localized type II membrane protein that lacks classical chitinase activity. Investigation of the global expression profile of wild-type and ''bc15'' plants, using Illumina RNA sequencing, further suggested a possible mechanism by which BC15/OsCTL1 mediates cellulose biosynthesis and cell wall remodeling. And it is localized in the Golgi apparatus with its C-terminus facing the Golgi lumen. | ||
Revision as of 12:00, 3 June 2014
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Contents
Annotated Information
Function
The rice Os09g0494200 was reported as Brittle Culm15 (BC15) which encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant chitinases, a class of glycosyl hydrolases, participate in various aspects of normal plant growth and development, including cell wall metabolism and disease resistance. BC15 was designated as Chitinase-Like Protein1(OsCTL1) and will henceforth be referred to as BC15/OsCTL1.
BC15/OsCTL1 is an N-glycosylated membrane protein. Mutation of BC15/OsCTL1 causes reduced cellulose content and mechanical strength without obvious alterations in plant growth. Biochemical assays demonstrated that BC15/OsCTL1 is a Golgi-localized type II membrane protein that lacks classical chitinase activity. Investigation of the global expression profile of wild-type and bc15 plants, using Illumina RNA sequencing, further suggested a possible mechanism by which BC15/OsCTL1 mediates cellulose biosynthesis and cell wall remodeling. And it is localized in the Golgi apparatus with its C-terminus facing the Golgi lumen.
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os09g0494200 |
|---|---|
| Description |
Similar to Chitinase-like protein (EC 3.2.1.14) |
| Version |
NM_001070084.1 GI:115479910 GeneID:4347451 |
| Length |
1807 bp |
| Definition |
Oryza sativa Japonica Group Os09g0494200, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 9:19801754..19803560 |
| Sequence Coding Region |
19802078..19802480,19802568..19802724,19802797..19803217 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008402:19801754..19803560 source=RiceChromosome09 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008402:19801754..19803560 source=RiceChromosome09 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgaagaggaagactcggaacaagataatcctgacgacgctgctggtgtcggcggcggcgatcctgatcggcgggacggtggcgctgatcctgacggcggggacatggaaggtgaagatgaaggagtcgcgcgagaagatctgcgacaaggggtgggagtgctcgggtagcaagtactgctgcaacgacaccatcaccgacttcttcaaggtgtaccagttcgagaacctcttctccaagcgcaacagccccgtcgcccacgccgtcggcttctgggactaccagtccttcatcaccgccgccgccctcttcgagcccctcggcttctgcaccaccggcggcaagcagatgcagatgatggagctctgcgccttcctcggccatgtcggctccaagacctcatgtggatttggtgtggcgaccggcgggccgacggcgtgggggctctgctacaaccacgagatgagccctaaggaggattactgcgacaagacgaacctgcagtatccctgcgtcgagggcgccgagtactacggccgcggcgccatccccgtcttctggaactacaactatggcgcggcgggcgacgggatacacgaggacctgctccaccacccggagtacctggagcagaacgcgacgatggcgttcatggcggcgatgtggcggtggatgacgccgatgaagaagaagcagccgtcggcgcacgacgtgttcgtgggcaactggaagcccaccaagaacgacacgctcgccaagcgcctccccgggttcggcgccaccatgaacgtgctctacggcgaccagatctgcggcaagggctacatcgacgacatgaacgtcatcatctcgcactaccagtactacctcgacctcatgggcgtcggccgcgagcactccggcgacaaccgcgactgcgccgagcaggccgccttcaacccctcctacaagaagcccgacgatcagcagcaacaaagctag</cdnaseq> |
| Protein Sequence |
<aaseq>MKRKTRNKIILTTLLVSAAAILIGGTVALILTAGTWKVKMKESR EKICDKGWECSGSKYCCNDTITDFFKVYQFENLFSKRNSPVAHAVGFWDYQSFITAAA LFEPLGFCTTGGKQMQMMELCAFLGHVGSKTSCGFGVATGGPTAWGLCYNHEMSPKED YCDKTNLQYPCVEGAEYYGRGAIPVFWNYNYGAAGDGIHEDLLHHPEYLEQNATMAFM AAMWRWMTPMKKKQPSAHDVFVGNWKPTKNDTLAKRLPGFGATMNVLYGDQICGKGYI DDMNVIISHYQYYLDLMGVGREHSGDNRDCAEQAAFNPSYKKPDDQQQQS</aaseq> |
| Gene Sequence |
<dnaseqindica>325..727#815..971#1044..1464#acacacaacggcaacgtcttaccagctcggagctctctcgcttgctgcctcttctcttcttcctcccgccaccgacaccacctccaccagcagcttcgccttcgccgcgtcggcatctgcagttgccacttcgctttcttccactccctcctcctcctcgcttacctcacactcctcccccccaatttcatcccccacccaccaccagatcccccaccacctgccgcattctcccccccaagatccagatcgccggtcacccccacgaactcgctcgagatccagagagagagagagaggcagtttcttggttgattttcgaggatgaagaggaagactcggaacaagataatcctgacgacgctgctggtgtcggcggcggcgatcctgatcggcgggacggtggcgctgatcctgacggcggggacatggaaggtgaagatgaaggagtcgcgcgagaagatctgcgacaaggggtgggagtgctcgggtagcaagtactgctgcaacgacaccatcaccgacttcttcaaggtgtaccagttcgagaacctcttctccaagcgcaacagccccgtcgcccacgccgtcggcttctgggactaccagtccttcatcaccgccgccgccctcttcgagcccctcggcttctgcaccaccggcggcaagcagatgcagatgatggagctctgcgccttcctcggccatgtcggctccaagacctcatgtgtgtagcttcttcgttttcgctatggtttgatttggatttgaggttttaagctcgccattgatggggatttgtgtgtgtgtgcaggtggatttggtgtggcgaccggcgggccgacggcgtgggggctctgctacaaccacgagatgagccctaaggaggattactgcgacaagacgaacctgcagtatccctgcgtcgagggcgccgagtactacggccgcggcgccatccccgtcttctggtaagcttcgcttcgtcgccgccatctcgatcgcttgatttgatgattgattcggattcggattcggaataggaactacaactatggcgcggcgggcgacgggatacacgaggacctgctccaccacccggagtacctggagcagaacgcgacgatggcgttcatggcggcgatgtggcggtggatgacgccgatgaagaagaagcagccgtcggcgcacgacgtgttcgtgggcaactggaagcccaccaagaacgacacgctcgccaagcgcctccccgggttcggcgccaccatgaacgtgctctacggcgaccagatctgcggcaagggctacatcgacgacatgaacgtcatcatctcgcactaccagtactacctcgacctcatgggcgtcggccgcgagcactccggcgacaaccgcgactgcgccgagcaggccgccttcaacccctcctacaagaagcccgacgatcagcagcaacaaagctagaacagatcacccccaattccccccaattccacggtaaagaattcacccaatgtgttgttgtatacatcttcttgctactgcaatcgctattgccaatgcaaaccaaccattgccattgctgttgcctcgtgagctatacagattaacggattcttcatcagtgtgaggagctgtggtttgcattggtgtatttctgagtagcggattttgagggaagtgtcaactgtgtgtgtgatgcctatgggatttgggaaatttgttgtagcccttgttgtaccatagtgtacattttcattccatttgatttttgtcacatatacatgtttaaagaggaggattcagg</dnaseqindica> |
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