Difference between revisions of "Os12g0189500"
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The increase in IAA content was strongly correlated with the expression of putative | The increase in IAA content was strongly correlated with the expression of putative | ||
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by | IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by | ||
| − | quantitative reverse transcriptase polymerase chain reaction. These results | + | quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results |
confirm the importance of the tryptophan aminotransferase/YUCCA pathway | confirm the importance of the tryptophan aminotransferase/YUCCA pathway | ||
in this system. All three genes were expressed in endosperm; expression | in this system. All three genes were expressed in endosperm; expression | ||
| − | of OsYUC11 appeared to be confined to endosperm tissue. Phylogenetic | + | of OsYUC11 appeared to be confined to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic |
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of | analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of | ||
| − | YUCCAs, which do not have orthologues outside the Angiosperms. This clade | + | YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade |
may have evolved with a specific role in endosperm. Expression of tryptophan | may have evolved with a specific role in endosperm. Expression of tryptophan | ||
decarboxylase in developing rice grains did not correlate with IAA levels, | decarboxylase in developing rice grains did not correlate with IAA levels, | ||
indicating that tryptamine is unlikely to be important for IAA synthesis in this | indicating that tryptamine is unlikely to be important for IAA synthesis in this | ||
| − | system. In light of these observations, we hypothesize that IAA production | + | system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production |
in developing rice grains is controlled via expression of OsTAR1, OsYUC9, | in developing rice grains is controlled via expression of OsTAR1, OsYUC9, | ||
OsYUC11 and that IAA may be important during starch deposition in addition | OsYUC11 and that IAA may be important during starch deposition in addition | ||
| Line 34: | Line 34: | ||
<references> | <references> | ||
<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref> | <ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref> | ||
| + | <ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref> | ||
| + | <ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref> | ||
| + | <ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for purification of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
Please input cited references here. | Please input cited references here. | ||
{{JaponicaGene| | {{JaponicaGene| | ||
Revision as of 12:49, 3 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Please input function information here. The increase in IAA content was strongly correlated with the expression of putative IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by quantitative reverse transcriptase polymerase chain reaction[1]. These results confirm the importance of the tryptophan aminotransferase/YUCCA pathway in this system. All three genes were expressed in endosperm; expression of OsYUC11 appeared to be confined to endosperm tissue[2]. Phylogenetic analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of YUCCAs, which do not have orthologues outside the Angiosperms[3]. This clade may have evolved with a specific role in endosperm. Expression of tryptophan decarboxylase in developing rice grains did not correlate with IAA levels, indicating that tryptamine is unlikely to be important for IAA synthesis in this system[4]. In light of these observations, we hypothesize that IAA production in developing rice grains is controlled via expression of OsTAR1, OsYUC9, OsYUC11 and that IAA may be important during starch deposition in addition to its pr eviously suggested role early in grain development.
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
| Gene Name |
Os12g0189500 |
|---|---|
| Description |
Pyridine nucleotide-disulphide oxidoreductase, class I family protein |
| Version |
NM_001072867.2 GI:297612801 GeneID:4351695 |
| Length |
8650 bp |
| Definition |
Oryza sativa Japonica Group Os12g0189500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 12:4507750..4516399 |
| Sequence Coding Region |
4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008405:4507750..4516399 source=RiceChromosome12 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008405:4507750..4516399 source=RiceChromosome12 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq> |
| Protein Sequence |
<aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq> |
| Gene Sequence |
<dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagttactccaattttggtccatgtgcatgcatcttaattttttttcttttgttttgaaatccggttgtcatagccatctcaatataggccaaaataagtttttttggtgtttctttccttattagccctaaaaataggcgacaaatgtccaaaattgcaccactgacctataaggatgggcccctagctaagaattaggtgcgactgagacgaggtaaagacctatatatatactaagacatgtaacaccttttttatttttctatttggaaatattcttttccctatttcataggaatgaactaaatttcaaaaaagaaaaagcacacaattactacaaaaaagtatgtgattgaaccattgaccaaagatggcgattattgtctttttcacttggcaaagtaggcaacataatccatctctcggccgccgcttggccatgtcaaactggcttagtgataaaggtcatccccttcgtcgattggctcacaagtgctagaaaaaggggaaaataggacatggcctttggcaggatgtgtttgttgtgtacttgcggtgtgggcggaaagtacaagaagcatgataattgggctgttcagtacataccgatgagtgaggcaacttgaggtagacaaagcaatgaagtgtatggctttggtggtaagattgagtagcctcgtcctccacatttgggtgaacaacaatgcttgatacatgaatatagaaaaagcattggacaatatgcataatggcatgaggcaataacactaacaagccacattgtagactgagatatatattgagtaccacaaccttgttgtaggtagcaaggtcagaacaacactgcaatacattgaaagacataattgactatctttcttggaattatgagcatgttgatacacattccactacagaacaaccattgttcttcaattacctgaataatttgatcctggcccattactggaaaaaataagatggatgagattgcatgcagtcatagttagtgctctggctaggttgctcgtgccaccaagatgcgagattagcacccgagagagtataatgtgtgaaggcacttcctgtgagctgagcctaagggagattacagtgcgtgaagttgcatctgaagtctcaggtctctcgcccacttatggttttgcataatcttcaggacttaacatatgtacctgatgcaagttcagctttgtagaagcataaaatatatgtgacacaagttcaatatgggcgcaactaaaaaacacaatactaactaattaaaagacgcttaattcttttgttcatcaagtctttgtaagagaacttgtcgatgcaaaaaaacataccaaatttggtcgttaacagaactgtacgaagtctttttcatgagagtttaagctaatgagatggctaataagcgagtgacctagtgcttatttctctgtgtcatattcagttgtgtgcgtggatgtatcacgtgacaagtgtttcgctcaacaacaatggtacatatatccaaatgacatagaatatgttgcttgaataaaacataactgatactcgcccaattggaaaattagtcaccgaagccctaccacagttggttttgttttgtacttttatttgagagaaatcaagagatagacaaggataaaagagtgaccagagcgatggtgattatagtgttaaattgtcgttaggaaatcactcaatgtttaaggggctgtttggttcatgatcctggtgaaaaggagcctggcctaatccctccctgtcacatggttgagtgttgtttggttcaaaggcctaaaggattttcaggatgcctgacttgaaccactgccttgcatcctttagcctgcgtccggcttcccattttggagctggcccgagcttcaggcacgtcaactccatgcatgcagttgcttgcgacattgtggtcggaataaaaaatttggcaatagtcccatctatattgattaaatcggaagtatgagcttgttttgattttgattcaaatccttagtttttttcctctaaattaatacttgttaattgctataattgaggagtacgagtttatcagtgttcattgttactgatagacaagagataatcgatgaatgatgaatcttccataacttaggtaagtgttttaccaacatatgtagtgtagtaaaaaaatgaattcaaccctttcgtgcttactataactaattaatttggttttctatttgctcctttaactctaaccctagctctatttattggcttgaacaaggtacatcttatttgaaatgaattgaatgaataaggcaaaaaagaaaaaaaatcattcttttggattcctggtatcctacaactctttttcacactacttacaaattctttttttcttggtcatggataaggctatacataaaatcaatgaatacgttaatatgtatctattaagttgatacatatcttaattaccaatgtgtaatcttgaactcaggctttcctagtcaagcatattttaccaaattctcaagtagctctcagacatacgtttttatcaatcatgttgctcaggtcactaaccaaacaagccctaagttgcaagaacacaaacacgactgatttcgtcaatgagcccaagtcgttggtgtttttaccagatgactcatcactatcggttcctttagagatgacactgatatcactgatggttcgtaattaattaacaagaaattactatgatacttaagtttccactaatgtatcacggttagttcataacaacaaccagcaccgatattcaatggaccagacctcaatggatcggatcatattcaagactatcatgtccattgagtatcaaactatatgtcattagtactcaaatgataatttgaaagaaaatggtaactttttcataagtcaggtgaagacaaacattatgtaaaagtatatagtttaaccaaagctacagctttgtagttgatgattttttgatttttgagattgtttacatgcccaagttgtcaaatctactcggacaaattttttgatattcccaaatgtccttggattgagactaactctacataaaaattggcgacacgatctattaatttttagttgacaatattttcattgtaagtcatttatatgcccgaatatatgttagaagtgttggctctatttggataaacgaaattttaaaatttttcaacgacttcagacaaacatgaactctatatggaagttgtggagatcgatgagatctacagatttgtcattgaagaccgaaaataatagcataagctatttaggcaaaaagagcattatagttgagatgggaggctgtggagatatagttgatcctatgtttgaatcctatgtacctcacacgcacgtacttcatgtgagaaatcatgtgaattgtgatttgtgatgtgtgtagatacttgatacgaaatacttttccaagtttttcatcgtggagagaattcaaaacccggtgcacaactatcaaatacatgttgatgtctttttgttgttctaactagtattaattgttcttaaataaagtggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</dnaseqindica> |
| External Link(s) |
- ↑ 1.0 1.1 Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.
- ↑ 2.0 2.1 Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188
- ↑ 3.0 3.1 Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513.
- ↑ 4.0 4.1 Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for purification of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825
