Difference between revisions of "Os10g0577600"
Wangzhen1991 (talk | contribs) (→Labs working on this gene) |
Wangzhen1991 (talk | contribs) (→Annotated Information) |
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===Localization=== | ===Localization=== | ||
| − | |||
JMJ706 is enriched in heterochromatin domains. | JMJ706 is enriched in heterochromatin domains. | ||
| − | To study JMJ706 localization in rice nuclei, a construct expressing a JMJ706 fusion with the GFP was used to transform WT rice plants. Nuclei of the transgenic leaf mesophyll cells showed an enrichment of the fusion protein in spots that overlapped with | + | To study JMJ706 localization in rice nuclei, a construct expressing a JMJ706 fusion with the GFP was used to transform WT rice plants. Nuclei of the transgenic leaf mesophyll cells showed an enrichment of the fusion protein in spots that overlapped with 4,6-diamidino-2-phenylindole (DAPI)–stained regions (Fig. 2B). To confirm this result, a construct expressing FLAG-tagged JMJ706 protein was used to complement JMJ706 |
T-DNA insertion mutant lines (see below). The FLAG-tagged protein was also observed in enriched DAPI-stained domains (Fig. 2C). | T-DNA insertion mutant lines (see below). The FLAG-tagged protein was also observed in enriched DAPI-stained domains (Fig. 2C). | ||
| + | |||
| + | ===Mutant=== | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 11:47, 4 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
JMJ706 encodes a heterochromatin-associated H3K9 demethylase involved in the regulation of flower development in rice. Histone lysine ethylation is an important epigenetic modification with both activating and repressive roles in gene expression. Jumonji C (jmjC) domain-containing proteins have been shown to reverse histone methylation in non plant model systems. Here, we show that plant Jumonji C proteins have both conserved and specific features compared with mammalian homologues. In particular, the rice JMJD2 family jmjC gene JMJ706 is shown to encode a heterochromatin-enriched protein. The JMJ706 protein specifically reverses di- and trimethylations of lysine 9 of histone H3 (H3K9) in vitro. Loss-of-function mutations of the gene lead to increased di- and trimethylations of H3K9 and affect the spikelet development, including altered floral morphology and organ number. Gene expression and histone modification analysis indicates that JMJ706 regulates a ubset of flower development regulatory genes.
Expression
Please input expression information here.
Evolution
A genomic survey revealed 20 and 21 jmjC domain-containing genes in rice and Arabidopsis, respectively (Fig. 1). The sequence alignment resulted in the discrimination of five groups containing the plant proteins. Groups I, II, and IV were found to be related to the JARID, JMJD2, and JHDM2 subfamilies, respectively (12). Group V could be divided into several subgroups, each of which was found to have related members of the human 'JmjC domain-only' subfamily (12).
Localization
JMJ706 is enriched in heterochromatin domains. To study JMJ706 localization in rice nuclei, a construct expressing a JMJ706 fusion with the GFP was used to transform WT rice plants. Nuclei of the transgenic leaf mesophyll cells showed an enrichment of the fusion protein in spots that overlapped with 4,6-diamidino-2-phenylindole (DAPI)–stained regions (Fig. 2B). To confirm this result, a construct expressing FLAG-tagged JMJ706 protein was used to complement JMJ706 T-DNA insertion mutant lines (see below). The FLAG-tagged protein was also observed in enriched DAPI-stained domains (Fig. 2C).
Mutant
Labs working on this gene
Dao-Xiu Zhou, Institut de Biotechnologie des Plantes, Universite´ Paris Sud 11, 91405 Orsay, France
References
Please input cited references here.
Structured Information
| Gene Name |
Os10g0577600 |
|---|---|
| Description |
Similar to PLU-1 protein |
| Version |
NM_001072024.1 GI:115483643 GeneID:4349499 |
| Length |
5887 bp |
| Definition |
Oryza sativa Japonica Group Os10g0577600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:23481090..23486976 |
| Sequence Coding Region |
23482626..23483016,23483139..23483295,23483373..23483592,23483708..23483761,23483848..23483927 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:23481090..23486976 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:23481090..23486976 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgcaacaggtggagggcaggaactgtcttcctgcggaggtcaggattggcctcgagacgctcaagaggcgccggcttgagaggatgcgtttgactgctcagaacaatgccggcgacggtcctccggtgcccgcaaggagcggtggggatgcgctaaggactcccgcaaactgcggggtcaggttgcatgctaacaatggcacagctctacctagcagaaccacccagaacaaggacccttttgcaaagcgcagggtggacaagtttgatatgtctagcctagaatggattgacaagatcgaagaatgccctgtgtactatcctaccaaggaggagttcgaggatcccattggttatatacagaagattgcacctgtggcttcgaaatacggaatttgcaaaatcgtatctccagtaagcgcttctgttcctgctggtgtcgtgttgatgaaggaacagcctggtttcaagttcatgaccagggttcagccgcttcgcctcgccaaatgggctgaagatgacacggtcactttcttcatgagcgaaagaaagtacactttccgggattatgagaaaatggccaacaaggtgttcgccaagaaatactcaagtgctagttgtctcccagctaagtacgtggaggaggaattctggcgcgaaattgcttttggtaaaatggattttgttgaatatgcctgtgatgttgatggtagtgctttctcctcttctcctcatgatcaacttgggaaaagcaactggaacttgaagaatttttcacggctttccaattctgtgcttagacttctgcagacaccaattccaggagtaacagatccaatgctttatatcgggatgctcttcagcatgtttgcttggcatgtggaagatcattatttgtacagcatcaattaccatcattgtggggcatttaagacatggtatggcataccgggtgatgctgctcctgggtttgaaaaggtggctagccagtttgtatacaacaaggatattttggttggtgaaggagaggatgcagcatttgatgttctcttggggaagacaacaatgttccccccaaatgtcttgttagaccacaacgttcctgtttataaagctgtgcaaaaacctggggagtttgtcattactttccctcgttcctaccacgcgggtttcagccacggcttcaattgtggcgaggctgtcaactttgctatcagtgactggtttcctctgggttctgtggccagcagacgctacgcgcttctgaacagaacacccttgcttgcacacgaggagttactttgccgttctgcagtgcttctgtcccacaaactgttaaacagcgacccaaaatccctcaataaatctgagcatccacattcacagcgttgtttgaagtcttgctttgtgcagttgatgcgattccagagaaacacacgtggcctacttgctaaaatgggctctcagatacattataagccaaaaacatacccgaatctctcatgtagcatgtgtcggcgtgattgctacattacacatgtgttgtgtggatgcaactttgacccagtctgtcttcatcacgaacaagaactccggagctgcccttgtaaatctaaccaggttgtctacgttagggaggacatacaggagctagaagctctatcaagaaaatttgagaaggatatttgcttggataaggaaataagtggttttgactcatacaagcaggccgaaaagaatgagccattttttgagataactcggaacctcaggaacactgaagtaaatttgatagaggatgccttctcaggagcaactgctgctgatgctgcaaagagttctcctgcaacgtcaacactgacatcttttgcacaacatgatgtgcctgttcttgctgaagcaattgtctgtgctaatcaagccgaccaattatactccaccaccgagcaaaccatcagctcacctttagtcaaaggaactgatgctgtgggtgcaaattcatccagcatggctgatgctaataacggaactggttcttgtaatgcttcagctgtggaatacagtggaaattcagattctgaatctgaaatatttcgagtcaagcgcaggtctggcgtatcagtaaagcctgcatctgatgccaagacatcaaacttgtctgatcaacaggttctcaggcggttgaagaaggtgcgccctgaaatacaacagcacaataagcgaccagaagactatggtcactgttcagttccctcaggtcgtatgagtatgaagaatttgaattcatcctcctcatgtggtgaagaacactggaggatgaagcggcggcagttggagactcagcaggatgagagcagttattctgcaaagcagaagtcgtactcgtatccatccaccagctattctttccgaggagagtttgtggaaatgagtagagatgctgctgcagaagtccgaccaaagcgactgaaaatccggctaccttcttctagcacgaacagagtggttgagcagggcagttcagggcaaagatttacaagggatgacaagtcgcttggttgttggcctgcaatttag</cdnaseq> |
| Protein Sequence |
<aaseq>MQQVEGRNCLPAEVRIGLETLKRRRLERMRLTAQNNAGDGPPVP ARSGGDALRTPANCGVRLHANNGTALPSRTTQNKDPFAKRRVDKFDMSSLEWIDKIEE CPVYYPTKEEFEDPIGYIQKIAPVASKYGICKIVSPVSASVPAGVVLMKEQPGFKFMT RVQPLRLAKWAEDDTVTFFMSERKYTFRDYEKMANKVFAKKYSSASCLPAKYVEEEFW REIAFGKMDFVEYACDVDGSAFSSSPHDQLGKSNWNLKNFSRLSNSVLRLLQTPIPGV TDPMLYIGMLFSMFAWHVEDHYLYSINYHHCGAFKTWYGIPGDAAPGFEKVASQFVYN KDILVGEGEDAAFDVLLGKTTMFPPNVLLDHNVPVYKAVQKPGEFVITFPRSYHAGFS HGFNCGEAVNFAISDWFPLGSVASRRYALLNRTPLLAHEELLCRSAVLLSHKLLNSDP KSLNKSEHPHSQRCLKSCFVQLMRFQRNTRGLLAKMGSQIHYKPKTYPNLSCSMCRRD CYITHVLCGCNFDPVCLHHEQELRSCPCKSNQVVYVREDIQELEALSRKFEKDICLDK EISGFDSYKQAEKNEPFFEITRNLRNTEVNLIEDAFSGATAADAAKSSPATSTLTSFA QHDVPVLAEAIVCANQADQLYSTTEQTISSPLVKGTDAVGANSSSMADANNGTGSCNA SAVEYSGNSDSESEIFRVKRRSGVSVKPASDAKTSNLSDQQVLRRLKKVRPEIQQHNK RPEDYGHCSVPSGRMSMKNLNSSSSCGEEHWRMKRRQLETQQDESSYSAKQKSYSYPS TSYSFRGEFVEMSRDAAAEVRPKRLKIRLPSSSTNRVVEQGSSGQRFTRDDKSLGCWP AI</aaseq> |
| Gene Sequence |
<dnaseqindica>1537..1927#2050..2206#2284..2503#2619..2672#2759..2838#2925..3202#3284..3685#3759..4081#4156..4419#5019..5426#ctctttcctcctccgccgcccgcccgcccattgccgagctccccgccgcacgccccagctaactaccactccggcacggcagggcagggcagggctcgccgccggaggggaatgtggcatccttaccttccccccttgccgtaaaacaaagaaagaaagtctcccgtctcggctccttgcgccatcgtcgacaggggaggggaggtggcgagcagtagcaggggaggggatcctccaggtgaccatcctctttctcacaaattcttctctctcccaccgggactacaatttgcttgcgttccttaatttcgcaccctcttttagatctggtgcaagaagctcttctcctccttaatttcgcaccctcctttaccgcaatactacaatctagtttgttgtctgttcccccccatccaatttaccctagattcctcatcaaatcacaccattttccctcaccctcctcagccccacggattaccgccttctttttcttactactaattgccgtccgtccgttgatgtttggaaaaagtaccacttttcttcttccttaatcttaatttgacgacgacgcgtagaggagaggaggtacagaaccaccggtatctaatcaaacagagacaagaaagttggaatcctaatttggccccgggtcgctgcaacagaatgcaagactagattgacacctagatttaccttaccttagaccgggccacaagtcaacgcgctcaattcatttcatttaccaccacaccactagtcgttcctccatcctggagtactactgtagtaggagtagtttttagtattcattactctctgtatggatgggatattactactactgtagtagagtagtcaagaggcggtcttccattcttgccgtgtggggttcagggggcatctcctctcctctcatctttcttttttgacgatttcttcgtctctctaaaatggcctggtcatatcgcatgtgcgtaattccccccctctgaccacgtccagtccactgcaaaaattttaaccttttcttctctttttctcttccaaggccctacatttttgtactactccacatttatatgcccttcttctcctcatcctcaatctgaatccgccgttattttctctctctaactcatgtaatggtttctgttcgtgtgagcacccaccattgccacgaatcgttagagatgcagcactgctaccattttttatacaactaaaatactaatacaattaattaatggctggttgattgtgacttctggctgtggtaggggaaaaccaccccaatgtgccgtgtctgaatccaatactgtagctttctttgacttggttgggacttgcccttctttcggtgctacctggctgtgaccaattcatacaaaccgttggtgcttgttcactctgctgtctctgttggtcaatgccactggccttcccagattttgcacatgaaccttcttcgttgctccttcattccgtaatgtgtccgtttacctatgcaacaggtggagggcagaaacacctatgcaacaggtggagggcaggaactgtcttcctgcggaggtcaggattggcctcgagacgctcaagaggcgccggcttgagaggatgcgtttgactgctcagaacaatgccggcgacggtcctccggtgcccgcaaggagcggtggggatgcgctaaggactcccgcaaactgcggggtcaggttgcatgctaacaatggcacagctctacctagcagaaccacccagaacaaggacccttttgcaaagcgcagggtggacaagtttgatatgtctagcctagaatggattgacaagatcgaagaatgccctgtgtactatcctaccaaggaggagttcgaggatcccattggttatatacagaagattgcacctgtggcttcgaaatacggtactgcttccgtttctcgttgccctcatcgtctgttgacagtgtattactgattgatctgccagtctacgcagaatcttactttctaaatgaatgatcatgggctattattttctccccaggaatttgcaaaatcgtatctccagtaagcgcttctgttcctgctggtgtcgtgttgatgaaggaacagcctggtttcaagttcatgaccagggttcagccgcttcgcctcgccaaatgggctgaagatgacacggtcactttcttcatgagcgaaaggtgtggtatttcttggttcattttcattattggctgcttatctcccaggtctcatgatgcttggcatgattttgcagaaagtacactttccgggattatgagaaaatggccaacaaggtgttcgccaagaaatactcaagtgctagttgtctcccagctaagtacgtggaggaggaattctggcgcgaaattgcttttggtaaaatggattttgttgaatatgcctgtgatgttgatggtagtgctttctcctcttctcctcatgatcaacttgggaaaagcaactggaacttgaaggtgactaactctcttctgtgtttgtgtgtgtggatgtgtcgtcacatgagtgcatgtttaaatatttgctcactggtagtatgtgttttactgatcgtatttttttgtttcgtagaatttttcacggctttccaattctgtgcttagacttctgcagacaccaattccagtaagtcatttatttactacttaatgtgtttcacattctttaacatggccaaaatttcatgattgtattacactcatccaaaacagggagtaacagatccaatgctttatatcgggatgctcttcagcatgtttgcttggcatgtggaagatcattatttgtacaggtataaattctcggttctccatggttactctttcttttttgctcgtaattaatactggtgttctgacattttattcatatgaccagcatcaattaccatcattgtggggcatttaagacatggtatggcataccgggtgatgctgctcctgggtttgaaaaggtggctagccagtttgtatacaacaaggatattttggttggtgaaggagaggatgcagcatttgatgttctcttggggaagacaacaatgttccccccaaatgtcttgttagaccacaacgttcctgtttataaagctgtgcaaaaacctggggagtttgtcattactttccctcgttcctaccacgcgggtttcagccacggtatgtatctttcagtttgtgcatgtgttataacgccactatcatatgcatagtattctaaggaatgtttcaactacccaggcttcaattgtggcgaggctgtcaactttgctatcagtgactggtttcctctgggttctgtggccagcagacgctacgcgcttctgaacagaacacccttgcttgcacacgaggagttactttgccgttctgcagtgcttctgtcccacaaactgttaaacagcgacccaaaatccctcaataaatctgagcatccacattcacagcgttgtttgaagtcttgctttgtgcagttgatgcgattccagagaaacacacgtggcctacttgctaaaatgggctctcagatacattataagccaaaaacatacccgaatctctcatgtagcatgtgtcggcgtgattgctacattacacatgtgttgtgtggatgcaactttgacccagtctgtcttcatcacggtaggaaatgttagcattgctcttttgcactttcttttgtattttacctaatactttggttattggactgcagaacaagaactccggagctgcccttgtaaatctaaccaggttgtctacgttagggaggacatacaggagctagaagctctatcaagaaaatttgagaaggatatttgcttggataaggaaataagtggttttgactcatacaagcaggccgaaaagaatgagccattttttgagataactcggaacctcaggaacactgaagtaaatttgatagaggatgccttctcaggagcaactgctgctgatgctgcaaagagttctcctgcaacgtcaacactgacatcttttgcacaacatgatgtgcctgttcttgctgaagcaattgtaggtttaccatcatgcatatatttccagctgttatctttctgttgggttaatgttcttgttattttatttaggtctgtgctaatcaagccgaccaattatactccaccaccgagcaaaccatcagctcacctttagtcaaaggaactgatgctgtgggtgcaaattcatccagcatggctgatgctaataacggaactggttcttgtaatgcttcagctgtggaatacagtggaaattcagattctgaatctgaaatatttcgagtcaagcgcaggtctggcgtatcagtaaagcctgcatctgatgccaagacatcaaacttgtctgatcaacaggtacgttaaattttcatcttttacacccctttggcttatcatcaacttttaggtactccctccggttctaaattaattgacgttttggacaaggttgagatcaattttttataactttgaccatcaataactttaaaaatatttagtttaaagaaactaaaacaacatatatagatttgtcttttaaagcactataataaaagtaaacatgtatttatttattatatatattatagtagaaaaataaggtcaaagatatatcttgtagaccgtgtcattgtccaaaatgtcaattaaaatggaaccggagggagtaattgcactgtttaagggaaaatacttcatgtaagaataaatgacatgctatataaatggccaggcatctccagttgtcgaggtcatttgatagataataatgggcaatcatgtcataaataaataagttgcagctctagttatgtattaattaagttcctgtaccattttacaccaaggtccaagggtctttatatgaaggatgacatacgggcccatagcatttagttgtcctattttagatagtgcttacttttggttctgatgtaaaatactggaatcaggttctcaggcggttgaagaaggtgcgccctgaaatacaacagcacaataagcgaccagaagactatggtcactgttcagttccctcaggtcgtatgagtatgaagaatttgaattcatcctcctcatgtggtgaagaacactggaggatgaagcggcggcagttggagactcagcaggatgagagcagttattctgcaaagcagaagtcgtactcgtatccatccaccagctattctttccgaggagagtttgtggaaatgagtagagatgctgctgcagaagtccgaccaaagcgactgaaaatccggctaccttcttctagcacgaacagagtggttgagcagggcagttcagggcaaagatttacaagggatgacaagtcgcttggttgttggcctgcaatttagaatttagaaaaaaatgctgacgcaggtgctaacctctgatggagataaagatttccttgtttaggtggggctgttgactacacgaaatgatagggctgggatggatcgatgaggttgaatataaatgattagcacagaagctgactgactttagaaggctggttaatcgattctttgtgcagcagtgcctccaatttgaggcacatctaggcacggttagaagagacagacacatgacgattcttgttgctggaggttctgggtcactgctttgctgctgtttgatgcctgcactgcaagtgacgtacacacactataggacctcgctctaccctttgattatatctctctatttttcttcttctttgtcagcaaccttagctgctgctactctatagctaactgtattctttcatacgcttgttccatcaagttaatctgctgcttaatgccattgattc</dnaseqindica> 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