Difference between revisions of "Os06g0600400"
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===Function=== | ===Function=== | ||
Please input function information here. | Please input function information here. | ||
| − | Rice CYP734As are multifunctional, multisubstrate enzymes that control the endogenous bioactive brassinosteroid content both by direct inactivation of CS and by the suppression of CS biosynthesis by decreasing the levels of brassinosteroid precursors.In addition, rice CYP734As can catalyze hydroxylation and the second and third oxidations to produce aldehyde and carboxylate groups at C-26 in vitro. | + | Rice CYP734As are multifunctional, multisubstrate enzymes that control the endogenous bioactive brassinosteroid content both by direct inactivation of CS and by the suppression of CS biosynthesis by decreasing the levels of brassinosteroid precursors.In addition, rice CYP734As can catalyze hydroxylation and the second and third oxidations to produce aldehyde and carboxylate groups at C-26 in vitro.Two P450s,CYP734A1/BAS1 and CYP72C1/SOB7/CHI2/SHK1, also act in the inactivation of brassinosteroids by means of different enzymatic activities. |
===Expression=== | ===Expression=== | ||
Revision as of 07:56, 6 June 2014
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Contents
Annotated Information
Function
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Rice CYP734As are multifunctional, multisubstrate enzymes that control the endogenous bioactive brassinosteroid content both by direct inactivation of CS and by the suppression of CS biosynthesis by decreasing the levels of brassinosteroid precursors.In addition, rice CYP734As can catalyze hydroxylation and the second and third oxidations to produce aldehyde and carboxylate groups at C-26 in vitro.Two P450s,CYP734A1/BAS1 and CYP72C1/SOB7/CHI2/SHK1, also act in the inactivation of brassinosteroids by means of different enzymatic activities.
Expression
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Expression of CYP 734A genes in wild-type and mutant rice.
Evolution
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Labs working on this gene
Please input related labs here. 1,overexpression of CYP734A genes in transgenic rice. 2,isolation of CYP734A1/BAS1-like genes from rice. 3,catalytic activities of rice CYP734As. 4,determination off the position oxidized by CYP734A2. 5,substrate specificity of CYP734A2.
References
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Structured Information
| Gene Name |
Os06g0600400 |
|---|---|
| Description |
Cytochrome P450 family protein |
| Version |
NM_001064535.1 GI:115468801 GeneID:4341450 |
| Length |
2534 bp |
| Definition |
Oryza sativa Japonica Group Os06g0600400, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 6:24581718..24584251 |
| Sequence Coding Region |
24581820..24582093,24582172..24582395,24582502..24582749,24582848..24583250,24583353..24583820 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008399:24581718..24584251 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008399:24581718..24584251 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgatggaggcggtggccgtggcggcggcggtgctgctgctgctgcacgtggcggcgagggtggcggacgcggtgtggtggcggccgaggcggctggaggcgcacttcgcggggcagggggtgcgcggcccgccgtaccggttcctcgtcgggtgcgtgagggagatggtggcgctcatggcggaggccaccgcgaagcccatgccgcccgccgcgccgcacaacgcgctccccagggtgctcgcgttctaccactactggaggaagatctacgggccgacgttcttgatttggttcgggccgacaccgcggctcacggtggcggagccggagatggtgcgggagatcttcctcacgcgcgccgaggcgttcgaccgctacgaggcgcaccccgtggtccggcagctggagggcgacgggctcgtcagcctccacggcgacaagtgggctcaccaccgccgcgtcctcacccccggcttctaccccgacaacctcaaccggctggtgccgcacgtcggcaggtcggtggcggcgctggcggagaggtggcgcgccatggcgtgcgccggcggcggcgaggtggaggtggacgtggcggagtggttccaggcggtggcggaggaggccatcacgcgcgccacgttcggccgcagctacgactccggccgcgtcgtgttccgcttgcaggcccgcctcatggcgttcgcctccgaggccttccgcaaggtgctcgtcccgggatacaggttcctgccgaccaagaagaacaggatgtcgtggggcctggacagggagatcaggcgcggcctggtccggctcatcggccggcgcagtggcggcgacggcggcgaggaagacgagaccaccaccgagctcaaagacaagcaggacagcggcttcaacgacttgctggggctcatgatcaatgccggcgtggacaggacgatgccggtggaggacatggtggaggagtgcaagaccttcttcttcgccggcaagcagacgaccaccaacctgctcacctgggccaccgtgctgctcgccatgcacccggactggcaggaccgcgcccgccgcgaggtcctcgccgtctgcggcgatgccgccggcgagctccccaccaaggaccacctccccaagctcaagacgctcgggatgatcctcaacgagacgctgcgcctgtacccgccggcggtggccaccatccgccgcgccaagttcgacgtcaccctcggcggcggtggcgacggcgacgccggaggcatccatatcccgcgcgacacggagctgctcgtcccgatcatggcgatccaccacgacgcccggttgtgggggcccgacgcggcccagttcaacccggcgaggttcgccagcggcgcggcgcgcgcggcgaagcacccgctcgccttcatcccgttcgggctgggctcccgcatgtgcatcggccagagcctcgccatcctcgaggccaagctcaccatggccgtcctcctccagcgcttcgacctcgcgctctcgcccacctacgtgcacgcccccaccgtgctgatgctgctccacccgcagtacggcgcgccgttgatcttccggccgcgccaatctcagccgtccaattag</cdnaseq> |
| Protein Sequence |
<aaseq>MMEAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGP PYRFLVGCVREMVALMAEATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPT PRLTVAEPEMVREIFLTRAEAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGF YPDNLNRLVPHVGRSVAALAERWRAMACAGGGEVEVDVAEWFQAVAEEAITRATFGRS YDSGRVVFRLQARLMAFASEAFRKVLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGR RSGGDGGEEDETTTELKDKQDSGFNDLLGLMINAGVDRTMPVEDMVEECKTFFFAGKQ TTTNLLTWATVLLAMHPDWQDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETL RLYPPAVATIRRAKFDVTLGGGGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQ FNPARFASGAARAAKHPLAFIPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPT YVHAPTVLMLLHPQYGAPLIFRPRQSQPSN</aaseq> |
| Gene Sequence |
<dnaseqindica>103..376#455..678#785..1032#1131..1533#1636..2103#aaacccccaacccaaccgccgccaccactcaccggcgcaaccaccggcggcgacctgatctctctgcgtttgtgtgctctgtttcaagaaacaggggaggagatgatggaggcggtggccgtggcggcggcggtgctgctgctgctgcacgtggcggcgagggtggcggacgcggtgtggtggcggccgaggcggctggaggcgcacttcgcggggcagggggtgcgcggcccgccgtaccggttcctcgtcgggtgcgtgagggagatggtggcgctcatggcggaggccaccgcgaagcccatgccgcccgccgcgccgcacaacgcgctccccagggtgctcgcgttctaccactactggaggaagatctacggtatgttgaggacggatggattttgtgtgcttgctcgtgtctgatcagatggattaatggcggtcgtcgcggttgcagggccgacgttcttgatttggttcgggccgacaccgcggctcacggtggcggagccggagatggtgcgggagatcttcctcacgcgcgccgaggcgttcgaccgctacgaggcgcaccccgtggtccggcagctggagggcgacgggctcgtcagcctccacggcgacaagtgggctcaccaccgccgcgtcctcacccccggcttctaccccgacaacctcaacgtgagtctctcctctgtttcttcatcctccgatcgatcggggcgcacccgcgatgacgacgacgacgatggctgacacgtgctctgtctctgtctctctcttgcagcggctggtgccgcacgtcggcaggtcggtggcggcgctggcggagaggtggcgcgccatggcgtgcgccggcggcggcgaggtggaggtggacgtggcggagtggttccaggcggtggcggaggaggccatcacgcgcgccacgttcggccgcagctacgactccggccgcgtcgtgttccgcttgcaggcccgcctcatggcgttcgcctccgaggccttccgcaaggtgctcgtcccgggatacaggtacggtacacgccacgaacaacccaaaaaactcccaaacgattcgccattctcgccgaaattgggctcacggttggcgccggaattcgatcgaacaggttcctgccgaccaagaagaacaggatgtcgtggggcctggacagggagatcaggcgcggcctggtccggctcatcggccggcgcagtggcggcgacggcggcgaggaagacgagaccaccaccgagctcaaagacaagcaggacagcggcttcaacgacttgctggggctcatgatcaatgccggcgtggacaggacgatgccggtggaggacatggtggaggagtgcaagaccttcttcttcgccggcaagcagacgaccaccaacctgctcacctgggccaccgtgctgctcgccatgcacccggactggcaggaccgcgcccgccgcgaggtcctcgccgtctgcggcgatgccgccggcgagctccccaccaaggaccacctccccaagctcaagacggtacgcacaccaaaccatatccatggcccagtgggggtattcccgtaattccacgccgacaaaagtctcaccttgttggttctcgctgtcatcttcttccagctcgggatgatcctcaacgagacgctgcgcctgtacccgccggcggtggccaccatccgccgcgccaagttcgacgtcaccctcggcggcggtggcgacggcgacgccggaggcatccatatcccgcgcgacacggagctgctcgtcccgatcatggcgatccaccacgacgcccggttgtgggggcccgacgcggcccagttcaacccggcgaggttcgccagcggcgcggcgcgcgcggcgaagcacccgctcgccttcatcccgttcgggctgggctcccgcatgtgcatcggccagagcctcgccatcctcgaggccaagctcaccatggccgtcctcctccagcgcttcgacctcgcgctctcgcccacctacgtgcacgcccccaccgtgctgatgctgctccacccgcagtacggcgcgccgttgatcttccggccgcgccaatctcagccgtccaattagctactattactaggtaggagtactagtattgctagctaggaaaagacaggaaagcccctgtccgttgtgtgcatagtgacacaggaaaaggccaagagacaactaacaaaaggctctgcccgtggcaggggcagggccggattccgaaaatgccctcgctaacagactagtggtacgatcagagcacaacttttagggatttgatgggtcagaaactctcagaagagaagaagagagacagtagtagggtagtagcagtagccccacacatccactgctcggttgcttccatttgttctcttctccttcgcgtggattctggtgtatgtatcgatcaatgcatgtacctgtatgtgtgtgttgtgtacaggggtgtgattattttgattcgggacacccggcacttcacagcaaaggagaattccatttgg</dnaseqindica> |
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