Os10g0497300

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Annotated Information

Function

RF5 plays a critical role in the assembly of the fertility restoration complex. The cloning of fertility restorer genes for CMS from petunia, rapeseed, radish, sorghum, and rice has identified PPR proteins as essential components for fertility restoration. Apart from the PPR13 restorer of fertility in sorghum, which belongs to the E type, all other RF-related proteins belong to the P subclass of PPR proteins. Their structural simplicity suggests the need for additional interaction partners to direct transcript binding and cleavage. PPR4 and PPR2 from maize chloroplasts associate with RNase-sensitive high molecular mass complexes that coprecipitate with ribosomes. In the same vein, Arabidopsis thaliana pTAC2 (for plastid transcriptionally active chromosome 2) was copurified with transcription complexes from chloroplasts. To date, very few direct protein partners of PPR proteins have been identified. The conventional model suggests that RF proteins process CMSassociated transcripts directly, owing to the RNA binding activity of PPR proteins. So far, the fertility restoration complex and its partner proteins have not been identified, and few results have shown direct interactions between the RF and CMS-associated transcripts. A Petunia RF restorer protein, PPR592, associates with a soluble, mitochondrial, RNase-sensitive, high molecular mass protein complex attached to the inner membrane. PPR-B has been demonstrated to associate with the orf138RNAin vivo by immunoprecipitation experiments. It remains unclear whether these interactions between the RNA and RF proteins are direct.In this study, we demonstrated that in mitochondria from the NILF1 hybrid, an RFC with a size of 400 to 500 kD functions in the restoration of fertility. These investigations provided evidence that RF5 is not involved in RNA binding and that RNA processing occurs via a GRP with RNA binding activity that is necessary for the restoration of pollen fertility. We postulate that the critical function of RF5 is to assemble the RFC in mitochondria, wherein Rf5 and GRP coordinate the processing of transcripts involved in fertility restoration in HL-CMS.

Expression

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Evolution

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Labs working on this gene

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References

Jun Hu, Kun Wang, Wenchao Huang, and et al. The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162. Plant Cell. 2012 January; 24(1): 109–122.

Structured Information

Gene Name	Os10g0497300
Description	Conserved hypothetical protein
Version	NM_001071493.1 GI:115482729 GeneID:4349007
Length	6882 bp
Definition	Oryza sativa Japonica Group Os10g0497300, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 10
Location	Chromosome 10:19392891..19399772
Sequence Coding Region	19392997..19395381
Expression	GEO Profiles:Os10g0497300
Genome Context	<gbrowseImage1> name=NC_008403:19392891..19399772 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008403:19392891..19399772 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggcgcgccgcgccgcttcccgcgtccgcgccggcgctgttggcgcccttcgctcggagggctcgacccaagggcgagggggccgcacggggggcagtggcgccgaggacgcacgccacgtgttcgacgaattgctccggcgtggcaggggcgcctcgatctacggcttgaactgcgccctcgccgacgtcgcgcgtcacagccccgcggccgccgtgtcccgctacaaccgcatggcccgagccggcgccgacgaggtaactcccaacttgtgcacctacggcattctcatcggttcctgctgctgcgcgggccgcttggacctcggtttcgcggccttgggcaatgtcattaagaagggatttagagtggacgccatcgccttcactcctctgctcaagggcctctgtgctgacaagaggacgagcgacgcaatggacatagtgctccgcagaatgacccagcttggctgcataccaaatgtcttctcctacaatattcttctcaaggggctgtgtgatgagaacagaagccaagaagctctcgagctgctccaaatgatgcctgatgatggaggtgactgcccacctgatgtggtgtcgtataccactgtcatcaatggcttcttcaaggagggggatctggacaaagcttacggtacataccatgaaatgctggaccgggggattttaccaaatgttgttacctacagctctattattgctgcgttatgcaaggctcaagctatggacaaagccatggaggtacttaccagcatggttaagaatggtgtcatgcctaattgcaggacgtataatagtatcgtgcatgggtattgctcttcagggcagccgaaagaggctattggatttctcaaaaagatgcacagtgatggtgtcgaaccagatgttgttacttataactcgctcatggattatctttgcaagaacggaagatgcacggaagctagaaagatgttcgattctatgaccaagaggggcctaaagcctgaaattactacctatggtaccctgcttcaggggtatgctaccaaaggagcccttgttgagatgcatggtctcttggatttgatggtacgaaacggtatccaccctaatcattatgttttcagcattctaatatgtgcatacgctaaacaagggaaagtagatcaggcaatgcttgtgttcagcaaaatgaggcagcaaggattgaatccggatacagtgacctatggaacagttataggcatactttgcaagtcaggcagagtagaagatgctatgcgttattttgagcagatgatcgatgaaagactaagccctggcaacattgtttataactccctaattcatagtctctgtatctttgacaaatgggacaaggctaaagagttaattcttgaaatgttggatcgaggcatctgtctggacactattttctttaattcaataattgacagtcattgcaaagaagggagggttatagaatctgaaaaactctttgacctgatggtacgtattggtgtgaagcccgatatcattacgtacagtactctcatcgatggatattgcttggcaggtaagatggatgaagcaacgaagttacttgccagcatggtctcagttggaatgaaacctgattgtgttacatataatactttgattaatggctactgtaaaattagcaggatggaagatgcgttagttctttttagggagatggagagcagtggtgttagtcctgatattattacgtataatataattctgcaaggtttatttcaaaccagaagaactgctgctgcaaaagaactctatgtcgggattaccgaaagtggaacgcagcttgaacttagcacatacaacataatccttcatgggctttgcaaaaacaatctcactgacgaggcacttcgaatgtttcagaacctatgtttgacggatttacagctggagactaggacttttaacattatgattggtgcattgcttaaagttggcagaaatgatgaagccaaggatttgtttgcagctctctcggctaacggtttagtgccagatgttaggacctacagtttaatggcagaaaatcttatagagcaggggttgctagaagaattggatgatctatttctttcaatggaggagaatggctgtactgccaactcccgcatgctaaattccattgttaggaaactgttacagaggggtgatataaccagggctggcacttacctgttcatgattgatgagaagcacttctccctcgaagcatccactgcttccttgtttttagatcttttgtctgggggaaaatatcaagaatatcataggtttctccctgaaaaatataagtcctttatagaatctttgagctgctga</cdnaseq>
Protein Sequence	<aaseq>MARRAASRVRAGAVGALRSEGSTQGRGGRTGGSGAEDARHVFDE LLRRGRGASIYGLNCALADVARHSPAAAVSRYNRMARAGADEVTPNLCTYGILIGSCC CAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTQLGCIP NVFSYNILLKGLCDENRSQEALELLQMMPDDGGDCPPDVVSYTTVINGFFKEGDLDKA YGTYHEMLDRGILPNVVTYSSIIAALCKAQAMDKAMEVLTSMVKNGVMPNCRTYNSIV HGYCSSGQPKEAIGFLKKMHSDGVEPDVVTYNSLMDYLCKNGRCTEARKMFDSMTKRG LKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPNHYVFSILICAYAKQGKVDQ AMLVFSKMRQQGLNPDTVTYGTVIGILCKSGRVEDAMRYFEQMIDERLSPGNIVYNSL IHSLCIFDKWDKAKELILEMLDRGICLDTIFFNSIIDSHCKEGRVIESEKLFDLMVRI GVKPDIITYSTLIDGYCLAGKMDEATKLLASMVSVGMKPDCVTYNTLINGYCKISRME DALVLFREMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVGITESGTQLELSTYNI ILHGLCKNNLTDEALRMFQNLCLTDLQLETRTFNIMIGALLKVGRNDEAKDLFAALSA NGLVPDVRTYSLMAENLIEQGLLEELDDLFLSMEENGCTANSRMLNSIVRKLLQRGDI TRAGTYLFMIDEKHFSLEASTASLFLDLLSGGKYQEYHRFLPEKYKSFIESLSC</aaseq>
Gene Sequence	<dnaseqindica>107..2491#tcccctctatcctccactgctgccgcccacctctccgtatcagctgcgtcgcgttggtttccgccggcgctgctgctgctgcaccagtccgctagggcgggcgggcatggcgcgccgcgccgcttcccgcgtccgcgccggcgctgttggcgcccttcgctcggagggctcgacccaagggcgagggggccgcacggggggcagtggcgccgaggacgcacgccacgtgttcgacgaattgctccggcgtggcaggggcgcctcgatctacggcttgaactgcgccctcgccgacgtcgcgcgtcacagccccgcggccgccgtgtcccgctacaaccgcatggcccgagccggcgccgacgaggtaactcccaacttgtgcacctacggcattctcatcggttcctgctgctgcgcgggccgcttggacctcggtttcgcggccttgggcaatgtcattaagaagggatttagagtggacgccatcgccttcactcctctgctcaagggcctctgtgctgacaagaggacgagcgacgcaatggacatagtgctccgcagaatgacccagcttggctgcataccaaatgtcttctcctacaatattcttctcaaggggctgtgtgatgagaacagaagccaagaagctctcgagctgctccaaatgatgcctgatgatggaggtgactgcccacctgatgtggtgtcgtataccactgtcatcaatggcttcttcaaggagggggatctggacaaagcttacggtacataccatgaaatgctggaccgggggattttaccaaatgttgttacctacagctctattattgctgcgttatgcaaggctcaagctatggacaaagccatggaggtacttaccagcatggttaagaatggtgtcatgcctaattgcaggacgtataatagtatcgtgcatgggtattgctcttcagggcagccgaaagaggctattggatttctcaaaaagatgcacagtgatggtgtcgaaccagatgttgttacttataactcgctcatggattatctttgcaagaacggaagatgcacggaagctagaaagatgttcgattctatgaccaagaggggcctaaagcctgaaattactacctatggtaccctgcttcaggggtatgctaccaaaggagcccttgttgagatgcatggtctcttggatttgatggtacgaaacggtatccaccctaatcattatgttttcagcattctaatatgtgcatacgctaaacaagggaaagtagatcaggcaatgcttgtgttcagcaaaatgaggcagcaaggattgaatccggatacagtgacctatggaacagttataggcatactttgcaagtcaggcagagtagaagatgctatgcgttattttgagcagatgatcgatgaaagactaagccctggcaacattgtttataactccctaattcatagtctctgtatctttgacaaatgggacaaggctaaagagttaattcttgaaatgttggatcgaggcatctgtctggacactattttctttaattcaataattgacagtcattgcaaagaagggagggttatagaatctgaaaaactctttgacctgatggtacgtattggtgtgaagcccgatatcattacgtacagtactctcatcgatggatattgcttggcaggtaagatggatgaagcaacgaagttacttgccagcatggtctcagttggaatgaaacctgattgtgttacatataatactttgattaatggctactgtaaaattagcaggatggaagatgcgttagttctttttagggagatggagagcagtggtgttagtcctgatattattacgtataatataattctgcaaggtttatttcaaaccagaagaactgctgctgcaaaagaactctatgtcgggattaccgaaagtggaacgcagcttgaacttagcacatacaacataatccttcatgggctttgcaaaaacaatctcactgacgaggcacttcgaatgtttcagaacctatgtttgacggatttacagctggagactaggacttttaacattatgattggtgcattgcttaaagttggcagaaatgatgaagccaaggatttgtttgcagctctctcggctaacggtttagtgccagatgttaggacctacagtttaatggcagaaaatcttatagagcaggggttgctagaagaattggatgatctatttctttcaatggaggagaatggctgtactgccaactcccgcatgctaaattccattgttaggaaactgttacagaggggtgatataaccagggctggcacttacctgttcatgattgatgagaagcacttctccctcgaagcatccactgcttccttgtttttagatcttttgtctgggggaaaatatcaagaatatcataggtttctccctgaaaaatataagtcctttatagaatctttgagctgctgaagccttttgcagctttgaaattctgtgttggagttcttttctcctacagttgtattagaggagggatcttctctttatgtgtaaatagcgaggtatgtatgtcacctctccgaattatttttactctggttcctagacggtaaacaagcaattatgttctgcctttgatgccagaaaaaacacaaaagtttgtcgttatctctactaacggatcataaaggaatttgtaactggagtttcaaacttaatttgtctaggcagtagttttggcattagatccaacattgtgtaggattcatttgtgtgtatcaatctatagggtttcattaaatttcgttaatgtgtactgtttaggtgttgaatagtttgacttgttttttaactgaacaaaagatactgaaatcgttccattcaacaaacacatgttccgttaatgaaattattgtacgttaccttttgttttcttactcacaagtgtcctcttttcttatatcctatagattggtacaacaaattattgattcaattttggttttgaacattgatgatcctccctgcactattggtgcagctgctcttctattcattttgtgaagtgatgtgagtacctctcaatcccatccttatgcttctgtgcatgcttcattccaattttttacgcatatcgattgttttcttttatataacagtccataaagataatcacatcatgacaaagttatttatttctacagtatagttatataagtattcaccagttttccatgaatattttggcatgtgattacaaagaagattatttgagaaaatccatgcttttatttcatcattttgtttgaagttgaactttaatttatggtgtaaatttcagttattattgctagcagctcgtactctttaatggtataacttcacttgtgcttattctccaatatctcccttcttgttgttcaggttcaagaaaatcatttgttggattcagaatctggtgtccattttcttcttaaattattaaatcctccagtgaatcttgttgattccaaagcaccatcgataggttccaaacttcttggaatcagtaaagttcaaatgcttaatggatcaaataaggattctgactgcatttcagaggaaatcctttcaaaagttgaagagattctcttaagctgtcaagtgatcaagtcgctcgacaaagatgacaagaaaacaacaaggccagaactgtgtccaaagtggcttgctttgttgacaatggaaaatgcatgcttgtctgctgtttcagtagagggtaagttttaatcaaatttcttggtcatgatttccctttatgaccattatatttatttatatgagccaaataagcagttgtcaacttgtcataagttacatagcacctatttgcaatattcatgggtggtttgcttagcccttttcttcacctgcttttgattgatgacttccatctgtgttgcagaattgaattggagtagtggactgcactagaagcacctatggccattgtcatactaggaaggttttcccttatcaaatatttgattgttacagagacttctgacacagtgtccagagttggaggaaattttaaagagacattaagggagatgggaggtcttgatagtatttttgacgttatggtggattttcattcaacattggaggtgagatctcgctaacatcgcatattttacatttcctttgttcaactctaatggattgtgcaggcttgttccttttcgccattttagctttaatgtgcttgaagccacatgaaagtaatgcttgtccagatacatagccaaaggttgttatattttggggcatggaaaatgcttgaggtagtaactattttcatcaggacatggaaaattggctgcaacacaaattatgttgttttatgttgcaaaaatagttttttaatacttttttattctgcatgtggtgttagtatcttacagttcctctgatgattatatcccccacgataataacacttgaaacgataataacacttgacatatctacaccaagtgaacattattcatttggatgttacttttccagctatacttgctgttcttgcatgtgtaagcaagtttggagtaaattgcgcattaatttaaatgcttggtgttcctatctgtgtactttttattccccaactaataatgcaatcatattacgctgataaactgaataaataaattaacaatatacttctggtggcaaaccttgtgtatcagaatctcataaaggatacatccacttcagctttggaccgaaatgaaggaacatctttgcaaagtgctgctctcctcttgaaatgtttgaaaatattggaaaatgccatatttctaagcgatgataacaaggtaatgctccttatatgttctgtttcagtttagtacccatttccttcttctgtactatcttctctcctgatttgttctgtgcaaaatgtgcaaacagtgcgactttgtatgtctgcttaacaattttcttttcttcctgaaaaagcaatatgaactcttacattcattttgcttcttgcagacccatttgcttaatatgagtagaaaattgaacccgaaacgctccttgctttcttttgttggtgtcattatcaatactattgagttattatcaggtatttttcttaataatacaatgtgttcgctaacacaataaaatgttttaaacatccagtatgttaaagttgcagtctgacgcctattttgttttgctgcagctctttcaatacttcagaattcttctgttgtttccagctctacatatccgaaatcgtctaaagtctctcaacagagttactctggtaataacaaacaccaattttgtttgatcagttgatctcgttggcttttctatgcactgtctcaatatagtttggtcgccattcaagtctcactacagatgttgaacttggcctgacaccaaatatttataaaatgctacctgatatttttaatatttcatgtttcctgacccagattatcttgttggttcctcgtataagtttaattagtgacattcttgaagctttgttatgcagcagatgtcatggggggaacttcatttaatgatggaaagagcaagaactcgaaaaaaaaaaacttttgtcgaaccagacacgtcattgttgcttatcttcaaaatcagaagtttctcatattactatatcttctggtagtgatgctggtctgtcacagaaggcattcaattgttctccatttatatcaagcaatggggcatcaagtggttcattaggcgagaggcacagcaatggtagtggtttgaagttgaatataaaaaaggatcgtggcaatgcaaatccaattagaggctcaactggatggatttcaataagagcgcacagttctgatgggaactccagagaaatggcaaaaagactccgtctatcttaaaatgtaatcaccgacagtggtggtggtgatgacccttttgcatttgaccgccgcgtcggcgtcgccaccacgtaatcgcccacgtcgctgcccccgctgccacgtcgtcgaccgcgcacggtaatcacacgcatctcgaggccgccgctagctgatatcttctcatccggttgatttgtgattttggcgtttttgcagtggtgatggcggggggcgaccgtggccgaggcgtggagtgccatccgcatcagggtgtatcggccgcgctgctccgccctggtccgcaggctttggcggcgagctggcggcggagggagactgtggtgagatcggatttcgccgctggtggtgtcgctaccatgggggattcgccgcaggcgctctcaggtttgcagcctcctccactctcttcccttttttatttttttttctcgcaaaatgtgttgtgatgttcgtctcgctgggcaggcctcatagccattaatgtagtttgctggaacatttacatttggaacgttgttggcaattgcttgacaaaatgtggaattgtggaggggagaaaaatcatttgaacctgcagtgacaaaattgccatctctaattttaaaactgaaggtgtggaaatcaaacataatcattgccagcgcatcattcttgttaaccaccatgatatattgttggttataacagttagctccacaccaaccttgaaggtgtcaatagaatgtttagtataaattgaggagaacaggcagttgttaagactttctaaagaacttgtagcagctaatactagctattgtgcatttgtgtttcatggaatttgagcagcaatggatatttcttactaagatgtatgatgcaaaacaaaaaactatgtctatacagtttacatgtaatgtgcggatgcaaataaaatcatgtacatgg</dnaseqindica>
External Link(s)	NCBI Gene:Os10g0497300, RefSeq:Os10g0497300