Os06g0160700

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Annotated Information

Function

Please input function information here. Four starch synthase I (SSI)-deficient rice (Oryza sativa) mutant lines were generated using retrotransposon Tos17 insertion. The mutants exhibited different levels of SSI activities and produced significantly lower amounts of SSI protein ranging from 0% to 20% of the wild type. The mutant endosperm amylopectin showed a decrease in chains with degree of polymerization (DP) 8 to 12 and an increase in chains with DP 6 to 7 and DP 16 to 19. The degree of change in amylopectin chain-length distribution was positively correlated with the extent of decrease in SSI activity in the mutants. The structural changes in the amylopectin increased the gelatinization temperature of endosperm starch. Chain-length analysis of amylopectin in the SSI band excised from native-polyacrylamide gel electrophoresis/SS activity staining gel showed that SSI preferentially synthesized DP 7 to 11 chains by elongating DP 4 to 7 short chains of glycogen or amylopectin. These results show that SSI distinctly generates DP 8 to 12 chains from short DP 6 to 7 chains emerging from the branch point in the A or B1 chain of amylopectin. SSI seemingly functions from the very early through the late stage of endosperm development. Yet, the complete absence of SSI, despite being a major SS isozyme in the developing endosperm, had no effect on the size and shape of seeds and starch granules and the crystallinity of endosperm starch, suggesting that other SS enzymes are probably capable of partly compensating SSI function. In summary, this study strongly suggested that amylopectin chains are synthesized by the coordinated actions of SSI, SSIIa, and SSIIIa isoforms.

Expression

Please input expression information here. In rice, there are 10 SS isoforms separated into five types; two granule-bound starch synthase (GBSS) isoforms (GBSSI and GBSSII) in the GBSS type, one SSI isoform in the SSI type, three SSII isoforms (SSIIa [SSII-3], SSIIb [SSII-2], and SSIIc [SSII-1]) in the SSII type, two SSIII isoforms (SSIIIa [SSIII-2] and SSIIIb [SSIII-1]) in the SSIII type, and two SSIV isoforms (SSIVa [SSIV-1] and SSIVb [SSIV-2]) in the SSIV type. Soluble fraction from rice developing endosperm was separated by anion-exchange chromatography with a linear gradient of 0 to 0.5 M NaCl. One peak of SS activity that eluted at 0.2 to 0.25 M of NaCl was detected in the presence of 0.5 M citrate and the absence of exogenous primers, whereas two SS activity peaks were detected at about 0.2 to 0.25 M and 0.35 M, in the absence of citrate but in the presence of glycogen primer. As in maize, SSI in rice exhibited SS activity even in the absence of exogenous primers, while rice SSIIIa activity was dependent on the exogenous primer (Fig. 1A). Native-PAGE/SS activity staining of each fraction from the HitrapQ column in gel containing rice amylopectin indicated that the strong activity middle band found in fraction numbers 3 to 5 corresponds to SSI activity.

Evolution

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Labs working on this gene

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References

Please input cited references here. Function and Characterization of Starch Synthase I Using Mutants in Rice. Plant Physiology, 2006, 140(3): 1070-1084

Structured Information

Gene Name	Os06g0160700
Description	Similar to Starch synthase I, chloroplast precursor (EC 2.4.1.21) (Soluble starch synthase 1) (SSS 1)
Version	NM_001187685.1 GI:297724500 GeneID:9269493
Length	7513 bp
Definition	Oryza sativa Japonica Group Os06g0160700, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza. Function and Characterization of Starch Synthase I Using Mutants in Rice. Plant Physiology, 2006, 140(3): 1070-1084
Chromosome	Chromosome 6
Location	Chromosome 6:3078297..3085809
Sequence Coding Region	3078848..3078960,3079791..3079803
Expression	GEO Profiles:Os06g0160700
Genome Context	<gbrowseImage1> name=NC_008399:3078297..3085809 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008399:3078297..3085809 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>aaaaatgctgtgggcattgcggatggcaatttcgacatacagggaacacaagtcctcttgggagggtctaatgaagcgaggcatgtcaagcgactttacatgggaccatgccgcctcacagtatga</cdnaseq>
Protein Sequence	<aaseq>KNAVGIADGNFDIQGTQVLLGGSNEARHVKRLYMGPCRLTV</aaseq>
Gene Sequence	<dnaseqindica>6850..6962#6007..6019#gtgccccaccgcctcagcgcctctccctcgcttaaaaccaaacccacacacccacctcttctctctctctcatcgtctccgcgactcagcccactcctctctctccaccaccaccaccaccaccaccaccgcccgccaagcgcggcccgcccgacacagcagcagcaggatcggcggagaggaggggggatcatggcgacggcggcggggatggggatcggggcggcgtgcctggtggcgccgcaggtgaggccggggaggaggttgcggctccagcgggtgcggaggcggtgcgtggcggagctgagcagggacggtgggtcggcgcagcgcccgctggcaccggcgccgctggtgaagcagccggtcctgccgaccttcctcgtgccgacgtcgacgccacccgcgcccacgcagtcgccggcgccggcgccgaccccgccgccgttgccggactccggcgtgggggagatcgagcccgatctagaaggtacgtggcttgtctcgtgctgatcctccctctcctcccgtatcaatgcccgatccgtgcagttccatgcgcgcctttgatcacttgattaatcgaacaaaccctgcggattaattaccacgcgagaaattggaaccccaattcgagcacatcactaacaatttttatactgcacatatacacaatatatatatttattggatcgcgatatgatgggggtggcgatggggtgggcacctgggaggtttcgaggcctgtgcacgtggctgggtttgagcagacacgccattgatgtagtagtatttgatatcgtaatttagtgggaagaggaatggtgaggtttggtgtaggaagcgttggaaactgagtaaattgacgactttgtcttttccttaggcaagtacggagtatatgcgatactcaagttgggagttcaattctcagccgtcaactgaattttctgtagagtgacagtagcatttcgtaattttcgttgcactgaattgtggtcgatggcgccattttctgtggacggtccatgtgtacctagacaatcacatgggcgcctgggaccaatatgtgcttttcctatcactagacccacctcgctatctgttggattctctgggctgccaccaatatgtgtttcctgattatatgacgtactctcacctcacctcacctctctaaaatccaattatttagagggcagaagggagcaatgacaacctgaatttggcgttattcagatcagaatgttgtttgcattattgtaatgctcccattactgctgtactgccatgccaagatgtggctagaaaacgataattatttatgtttctgctgtatttttcttgggcctctcggccaatactataccatatgaatatcttttattttgcattcaggtctcacagaagattccatcgacaaaacaatttttgtggctagtgagcaggagtctgagatcatggatgtgaaggagcaagctcaagctaaagtaacacgcagcgttgtctttgtaaccggtgaagcttctccttatgcaaagtcaggtggactaggagatgtttgtggttcactgccaattgctcttgctcttcgtggtcatcgtgtgatggttgtaatgccgagatacatgaacggggccttgaacaaaaattttgcaaacgcattttacactgagaagcacattaagattccatgctttggcggagaacatgaagttactttttttcacgagtatagggattctgttgattgggtgagtattcaatctccttactattttctgatgaaaacaactatctgcttatcttttttgttcttatcccactgaagacatgatcatatcatattctgtactctgtttctatgtcttactgtctatacacttgtgcactggttgatgtctggtgtttccagatcaaaatgcaaaagattccttcatatgacttaatgtatccattttatgtctcaagcatttgtgacttctgtttgcaacaagcacttaatattaataagttgtactgtttggcttttccattgcattaatagtcatcagtaacattgtagttatatcatgggaattatgtacttcaatcgcatttttatttcttgaaagtgaacatgtgggttacattgaaacatgtattgtcacaactggtaatagatgtagatggcatgacatgataatcattaaggaacaaggatgtgtataacctaggaggaaacatttctagcatttttggttgcaagattgattccttgttgagtttaccaaatgtttacttattcctattggaacttggtagtgttcactccgcagcaccatcacccatgacacaacatgctgattgtagaaagcatacctgcattttgggtacttatttttttgttacacctcacttctcctgttcaggtgtttgttgatcatccctcatatcatagacctggaaatttgtatggagataattttggtgcttttggcgataatcaggtattcttacatcaagatcctatttgaccagtctaaattatatcacattgagtcattgattcgacttgacttccctatatactgcagttcagatacacactcctgtgctatgcggcgtgtgaagccccattaattcttgaactgggaggatatatctatggacagaaatgcatgtttgttgtgaatgattggcatgccagtcttgtgccagtgtatgttctggatctgaaactctgcaaccaaattgattaacattgaatatctcttataaaccatttttttcatacgtttgctttgtgtttgtgtgtgtctatgttagataagaggaacaaaatattagattgagtgaataggaggattttctcaatcattttttttctcaaaagcgcaggagaactgcgattcatttcattattttctcaatcattttgatatttccaaaaacatgaacagtccagtacatcatttatgtgtttaaatgatttagttttgtgctacactagttcatagctgatctgaaaatctccctgcctatgttttttttggaatatttctgagctagtgcgaagtttcttattatggtagtgcctattttgagttcatcttctacagatacaattcaatacccaaaggatttgggaaaaatcctgtagtctaaatggcctatcatgctactaggtgactgaggtgatagtttgcaaccctctaatttgtggtaaatttaaactgcttgtttcacaccataatttgatgttataattattaagagagtgcgtggtacatgctttctaacttctaagacacttcagaacaacatgaggttaatggtgtaatgtctttcgctgcatattatgaatatggtttgatccatcagatgctacattgctactcctttgttttggcatgttcctcacggtggataagatactaatgccagtgaaaaactcatcacacttattttttagtccctttttacttcaatgctgaatgttaaattggttttccttacatatcaatttaaatataacttttatcttgtttatccagccttcttgctgcaaaatatagaccatatggtgtttacagggatgcccgcagtgttcttgtcatacataatctagcacatcaggtttgagcctatcattttgcatatcttttaagtagttttgtaatggcactttgtaagtgcagtatgttagtatttcattgtgtttcaatgtgatagggtgtggagcctgccagtacatatcctgacctgggattgccacctgaatggtatggagcattagaatgggtgtttccagagtgggcaaggcggcatgcccttgacaagggtgaggcagtcaattttttaaaaggcgcagttgtgacagcagatcgaattgtgactgtcagccaggtgagattccttccttttcgtctactagttggcttacggccacagtcaaaatttgaaatttgaacttaaccttagagttgatttttttttcccatcctagtctatttttatgccttggcttttaaaccgcaaattcttcgttcattttgtcttgcactcaattaaggaatcatttatgtttcattaccaggggtattcatgggaggtcacaactgctgaaggtgggcaaggcctcaatgagctcttaagctcccggaagagtgtattgaatggtacctatatttgggtttttatattttgttcttggactgatcattgtaatagggtggatgatacaaattaattcaatctattccctggatatgtaggaattgtaaatggaattgacattaatgattggaacccatccacagacaagtttctcccttatcattattctgttgatgacctgtccggaaaggtgaagtatagtaccatgtatatacagattgtcatctcgtttcatttttttctttgcacctcgtcgtttctttcacagactaatttatgaaacctcgaattcatgtttaggccaagtgtaaagctgaattgcagaaggagctgggtttacctataaggcccgatgtgcctctggttagatacaaatatttatgaaattatttattttagtattcttttatgggcagttggatttattagctttatttacagattggctttattggaagattggactatcaaaaaggcattgatctaattaaacttgccattccagatctcatgcgggacaatattcaattcgtaagttctgttttctttttctgcgtgagactagaaagtgaatgtgatagatcttagtgtatatagtagtatattgacacttatatagcttgttatcatgaatctaaggaatgtaatatgcagctattgctttcattgtgaacgcatatgcaagtaaactagtatttcagatgcaaatattagtgctataagaaaaaatgcatatgcaagtcataaaagaaagatagcagtagtataacttgaccactgtatgacttattagcaaccatccagtgtgatatcaattggagcacctgcttggccagccagtctgttatttccaccctttatacattttgggtacactaaacaacatcttctgacagacaccaggggacgcgtatacatgcagacaaaaggatcaccatttattaagtcagtaggatctaacttgtaggcagcaaatgtcttgatgaactgcaaccacgtgcttatcccaaatacttcattgttgggggtttctggctttcaactggagactaaaacagagtagtgtttgtagtgggttttaaaaaattttatccacatattttagccaacaactgaaatcgattgatcaatgacaccttttaatacttctttttttcttaaaaaaaatgacaccttttgttttttttgttataggtcatgcttggatctggtgacccaggttttgaaggatggatgagatccacagaatcagggtacagggataaatttcgtggatgggttggatttagtgttccagtttcccaccgaataactgcagggtatgcaaaataacttcgccaataatagtcatttgtcagctaaggatgcatttacatttgaaatctcaattaattttgctgataaagttacatttgaaatattgatgcctctatttttgtacagttgcgatatattgttgatgccatccagattcgaaccttgtggcctcaatcagctatatgctatgcaatatggtacagtgcctgttgttcatggaactggaggcctcagagtaagctttcctatagtgtgacacttatgtcagcacagcacccaaggcagagctgctcacttttttttatttattcaacaggatacagtggagaattttaacccgtttgctgagaaaggagagcagggtacagggtaaggaatgcttcattaaagtaaaattgcagatttattttattttagtggaattaattaaattcaatttttttttgtatattattcaatttataaccaattaatttaactgttgctacttgggacatattgcaggtgggcattctcgccactaaccattgaaaaaatgctgtgggtaagtttatgttgaacctgcacctagttttagttcttctatccattccttaatcccattatatatgccatacaacatcaatactattggccctcttaacatcaatagcatgatgatttcaactatgcattttggttgtatcgtatactgctatttttcttgccggtaatcataatgtagtttgattcttgccagaagtaaaaaggataaaacaaggtgaggctcatgacgtatgttccagccagttctggttattgtctggtattccagtaggattgaactcctagtacttgttagttttcagatttaagtaaatattataagaaagagttgacaagcaaactaaagaaaaggaacacacgtgattgaaatccaaagaagaaagcggctcagcacttggtgggttaatgggaaactggaattagtggggaatagaaccctgaagcatatatgtgcttaggttgctaaattgctcgactgaccagtccatgatttgaattgcatgtttggtacatacttcgaatgtggaaacagggaaacagggcccattttgtgtcattgaacacttcaaatgaaactgataaaaaaatggatttgacaaggaaagatttgcattgcttgtctacaatttaatgtgagtactagtcagttttgttttgccttgtactcaagtagtcaagataataattagggaaagctacaaactttgttgagaaaacattgtagaatcagtaataaaagcctcattgaaaatggtattttcagggcacaagcattacaccatatgaacttttggagtatgtactaaaaatttgcatcgtttcttgcaggcattgcggatggcaatttcgacatacagggaacacaagtcctcttgggagggtctaatgaagcgaggcatgtcaagcgactttacatgggaccatgccgcctcacagtatgaacagatcttcgaatgggccttcatggatcaaccatatgtcatgtaaatggatttgaaggaagcagcgaatttctccgaggaccctcaatcttcctgtctttcatgagcggaatgaaaactttgtacactacatggaaagggaaccagttatgcaaagttgcaaacgatcactcaaggttacccttgtaggcctgctacttggccaatatggttccagtgaccatatgcagagtcaggttcagatgaatggcacttgtgagtagtgaagaataagatgaggatgcttgaagcggtttcacatgtggctgataccacgcaagcaacctctcaatgcatcgaaatgtgagtcttggaatcaataggatttagctcccatcaattacagttgtacccttttttgcttaatactttgtcgcctgtgctgttcttatatttgtgtgaagataaattttagtccattaggtaactgtattgttgagtcttaaggtgaagactaaatagtgtttggaagctgtagctactgcgatgtcaagtgtcaaaagagatcttgg</dnaseqindica>
External Link(s)	NCBI Gene:Os06g0160700, RefSeq:Os06g0160700