Os11g0587000

Annotated Information

Function

It reports here the molecular genetic characterization of dwarf27(d27), a classic rice mutant exhibiting increased tillers and reduced plant height.dwarf27 encodes a novel iron-containing protein that localizes in chloroplasts and is expressed mainly in vascular cells of shoots and roots. The phenotype of d27 is correlated with enhanced polar auxin transport. D27 is involved in the MAX/RMS/D pathway, in which D27 acts as a new member participating in the biosynthesis of strigolactones.Tillering in rice is one of the most important agronomic traits that determine grain yields and a model system for elucidating molecular mechanisms that regulate axillary buds (Wang and Li,2005). In this study, we characterize a rice dwarf 27 (d27) mutant that is defective in the outgrowth of axillary buds. Map-based cloning and in-depth analysis of D27 revealed that it encodes a novel chloroplast-located iron-containing protein. Our results demonstrate that D27 regulates tiller bud outgrowth through the MAX/RMS/D pathway and participates in the biosynthesis of strigolactones. D27 Encodes a Novel Iron-Containing Protein Sequence analysis of 5 9 - and 3 9 -rapid amplification of cDNA ends (RACE) products indicated that the full length of D27 cDNA is 1254-bp long, with an ORF of 837 bp, a 217-bp 5 9 -untranslated region, and a 200-bp 3 9 -untranslated region (see Supplemental Figure 2 online). Sequence comparison between genomic DNA and cDNAs revealed that D27 is composed of seven exons that encodes a 278–amino acid polypeptide (Figure 2C; see Supple- mental Figure 2 online). The 4-bp deletion in d27 results in a premature translational product (Figure 3A; see Supplemental Figure 2 online). The BLASTP (Altschul et al., 1997) analysis revealed that D27 shares no homology with any functionally identified protein and contains no conserved domain. However,analysis of multiple alignment against the National Center for Biotechnology Information database and The Institute for Genomic Research (TIGR) plant transcript assemblies showed thatD27 has homologies in many plant species, from lower plants to higher plants (see Supplemental Figure 3 online), suggesting that D27 may play a basic role in plants.Interestingly, when we tried to express and purify recombinant D27, we found that the bacterial cells expressing the maltose binding protein (MBP)-D27 fusion protein were strikingly brown in color, as was the purified MBP-D27 fusion protein (Figure 3B).This result suggested that D27 is very likely to have a cofactor. To explore this possibility, we analyzed the recombinant D27 with inductively coupled plasma mass spectrometry (ICP-MS) and found that the recombinant MBP-D27 protein contains ; 1.7mole of iron per mole of protein, in contrast with an extremely low level of iron bound to the C-terminal truncated polypeptide, MBP-D27 1-187 (Figure 3C, Table 1), which is equivalent to the mutated form of D27. The binding of iron to D27 was further confirmed by characterizing the absorbtion spectrum of the MBP-D27 fusion protein, which showed a specific peak at 420nm, a characteristic for the presence of iron (Figure 3D). Fur- thermore, when the recombinant MBP-D27 protein was treated with the reducing agent dithionite, the peak at 420 nm exhibited a dramatic decrease (Figure 3D), indicating that D27 is indeed an iron-containing protein. Moreover, the purified recombinant D27 protein contained no significant amount of other metals (Table 1), suggesting that the binding of iron to D27 is specific.Taken together, all these results indicate that D27 is an authentic iron-containing protein in plants.

Expression

In rice (Oryza sativa), the plastid-localized protein DWARF27 (OsD27) is necessary for SL biosynthesis, but the equivalent gene in Arabidopsis has not been identified.And DWARF27 (D27) have been identified with reduced strigolactone levels or strigolactone response.

To isolate the D27 gene, we took a map-based cloning approach.D27 was primarily delimited in an interval of ; 3.0 centimorgans between the two molecular markers C189 and RM206 on the long arm of chromosome 11 (Figure 2A). To fine-map the D27 locus, we generated a large F2 mapping population derived from a cross between d27-ZF802 and its isogenetic lines ZF802. Of 21,000 F2 plants, 5200 mutant plants were used for fine-mapping, and the D27 locus was located between the two cleaved amplified polymorphic sequence (CAPS) markers P1 and P7 (Figure 2B). Screening with newly developed molecular markers P1 to P7 (see Supplemental Table 1 online), D27 was further placed within an 18-kb DNA region between the P3 and P6 markers and cosegregated with the P5 marker (Figure 2C). Within this region, there are two open reading frames (ORFs).Sequencing of these two ORFs of d27-ZF802 revealed a 4-bp deletion at the fourth exon of a putative gene, ORF LOC_Os11g37650, and this deletion results in a frame shift and generates a premature translation termination product (see Supplemental Figure 2 online).The identity of D27 was further confirmed by a genetic com-plementation test. The plasmid pD27C, containing a 9.25-kbgenomic DNA fragment consisting of a 2236-bp upstream se-quence, the entire D27 gene including seven exons and six introns, and a 2044-bp downstream region (Figure 2D), was introduced into a d27-Nipponbare mutant. All four transgenic lines of pD27C complement the d27 phenotype (Figure 2E).Therefore, ORF LOC_Os11g37650 is the rice D27 gene, and its 4-bp deletion is responsible for the altered phenotype of d27. Expression Patterns of D27 and Subcellular Localization of the D27 Protein Real-time PCR analysis revealed that the D27 expression level is high in axillary buds and young panicles, medium in shoot bases and culms, and low in roots, sheaths, and leaves (Figure 4A). The tissue-specific expression pattern of D27 was further examined using mRNA in situ hybridization. D27 was predominantly ex- pressed in young leaves (Figure 4B), axillary buds (Figure 4C),inflorescence promodia (Figure 4D), lateral roots (Figure 4E), and crown roots (Figure 4H). Furthermore, D27 expression was detected in vascular cells at the shoot apex of the main stem and young leaves (Figures 4F and 4I), in the nodal vascular anastomosis (Figure 4G), and in large and small vascular bundles of the internodes (Figure 4J).To determine the subcellular localization of the D27 protein, we performed a transient expression experiment of D27 in rice leaf protoplasts. The C terminus of D27 was fused with green fluorescent protein (GFP) under the control of cauliflower mosaic virus (CaMV) 35S promoter, and the construct was transferred into rice leaf protoplasts by the polyethylene glycol–mediated method. In contrast with the control, which was ubiquitous in protoplast cells, the D27-GFP fusion protein was predominantly localized in chloroplasts

Evolution

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Labs working on this gene

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References

1.Wang Y, Li J. Branching in rice[J]. Current opinion in plant biology, 2011, 14(1): 94-99. http://www.ncbi.nlm.nih.gov/pubmed/21144796 Waters M T, Brewer P B, Bussell J D, et al. The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in the control of plant development by strigolactones[J]. 2.Plant physiology, 2012, 159(3): 1073-1085. http://www.ncbi.nlm.nih.gov/pubmed/22623516

Structured Information

Gene Name	DWARF27
Description	Putative uncharacterized protein
Version	FJ641055.1 GI:254946545 Gene ID: 3974662
Length	5390 bp
Definition	Oryza sativa Japonica Group DWARF27, complete gene.
Source	Oryza sativa Japonica Group (Japanese rice) cultivar = Nipponbare ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza. Chromosome = Chromosome 1
Chromosome	{{{Chromosome}}}
Location	Chromosome 11:222..5190
Sequence Coding Region	222..525,2082..2221,2438..2530,2891..2952,3514..3620,4977..5066,5150..5190
Expression	GEO Profiles:DWARF27
Genome Context	<gbrowseImage1> name=JaponicaChromosome11:222..5190 source=RiceJaponica11 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=JaponicaChromosome11:222..5190 source=RiceJaponica11 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>ATTCCCACCACAACCAAGATGCCCTCTCCATGCCATTTGGTCTCTTCTCTCTCCCTCCTTGCAAATTGCATGACCCTCTCTCTCTCTCTCCACTTCTCTCTATAAACCTTCCTCTCTCCCATAACTTCTTTCCATTTTCAACCTACAAATATACTAATCTCTCTCTAGCTAGTCTTCACCTACAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCATGGAGACCACCACGCTTGTGCTGCTTCTTCCTCATGGCGGCGCCGGCGGCGTACGGCCGGCGGCAGCGGCAACGGCGAAGCGAAGCTACGTGATGAGGAGGTGTTGCTCGACGGTGAGGGCGGTCATGGCGAGGCCGCAAGAGGCGCCGGCGTCGGCGCCGGCCAAGAAGACGGAGACGGCGGCGATGATGTCGACGGTGCAGACGGAGACGGCGGCGGCGCCGCCGGCGACGGTGTACCGGGACAGCTGGTTCGACAAGCTCGCCATTGGTTACCTGTCCAGGAACCTTCAAGAAGCTTCTGGTTCGTGCCTATAATCTACTGAATATATCTGATGTATTGTGTGCATGTACATGCAATCTTTCGGATGAATTAATGAATGATCAAATCAACCATGCATGCATATATGTGTGTATTTATGATGTTTTTGCCATGGCTGATTTGATTTTTTGAGTGGAAGAAATAACATATATATGGTGAATAAACCAATGTTCTTCAGTTATTTTTGTGACAATTAAATTATATGTGTATTCTACGATCATCCTTTTCTTCTTATCGTTTGGTTATTAATACCCTTCTCGTCCATGTGGAACATAAATTCTTTGATTTTTTTTATTACATGTTGCTTTGGCCAGCGTGGGTGTACTTGCAATTGCATGCTTTCTGTCATATTATAATTGAATTAGCTCTTGATTTTTACATGCAAAAGAATAATTTATTTATTGAGTTGCTGATGATCACGTAAGCCTATGCATGTGAGATGTGATGTGTTCTCCGGCCTAATTAAGTATCGACGTACTATATATATGCACGTACATCAAGTACGGCCGTATTTCATAATTTGATTATTGTTACTGTACCAGACAAACCTAGTGCTTAATTATTGACATATACCCAACATCGATTAAAGTCAGTGTGACACAATTGGCAGAAGAAGATAACAAAAATCATAGAAGACATATATACCACAACAGTAAAAATTACAGTGCAAAAAGGGATACCAGAAGCTTCTTAATTAGGTGAATGGTCACATTTAGTAGTAGTAATACACAGTAATAACACATTATTTGACGAATAAATTAAGCGGAAGTGCATGATCAGCAGCATATAAGCAAGCAACTACGAGAGGTCAACCGTTGATCCGTGAAAATGTGAGGTTCACATTCTAGGAATCTTTTAGTGCTTGAATCTAATCCTTTCGTTTGTTTTCCCGTGGACTTTTTACTTTGATCGAATAAACTTATCTGAATAATGCGAAGGTCAAACATCACATGTACGTGCCCGGTTCTTCTTCTAGAAATTCAGCCGCTTACTATAATCGATGCTGGTTTTTGAATTTTTTTAAAGACATTTATTTTGGAACAACTTAAAAAAAGTGCACAAACCAGGGTTTACCAAACCGTTTGAAGTGAGGTTACTGCCACTCCATGGTTTGGCAATTATCACGAGGTGCGTCGTGGTTCCAAAAACTAATAAGGTTATCGTATGTGATAACCGCGATAACTGACGGTTTTGTAAACCCTGCACAGACAACATAATTATATTTACAGGACTGTTTGGACTTCATTAACACTGTAGCATCCACTACATCCGTCACCTCCTTGGCTCTCTTACATGTTTAGCAAAGTCAATCATACAGACAAACCAGAAAAGTTCCCAACAGACACAGAATGCTACTTTTCTAGTATGGGTAATTAAGCATATAAGAGAAACTTGAAAGCATACAGGAAACCGATGTTCACTAGTCCTGAGTTTTTTTTTGGTGTTGTTCAGGTGCAAATTAACTGCAAAGGTTGATTCCCTGAAAGGAACTTGTAGCACTTGCTGACTGACACGAACATGCATGCAATGTAATGCAGGGCTAAAGAATGAAAAGGATGGCTACGAGAGCCTGATAGATGCCGCCCTAGCCATCTCAAGAATCTTCAGTCTGGATAAACAAAGCGAGATTGTGACCCAAGCTCTTGAAAGAGCACTTCCAAGCTACATCCTCACAATGGTAAGTACCATAATCCATGACAATTGGCAATCATGTATGAATTATTGAAATTTAAAAAACCTAAAACTTTTTTTTATTTGAACAGGAAGTAAAATTCAGCATTTATTCTCTCTTTTTTTTTTGAGGAAAATACAGAACTTCTGTAAAGGGAGGAAACTTTAAAAGTTCTCTTCTTTTATTTCTTACCTCAACTTTGATTGAGAATTCTGTCGGCAGATCAAGGTGATGATGCCACCTTCAAGATTTTCCAGGGAGTACTTTGCTGCATTCACCACGATATTTTTTCCTTGGTTGGTTGGGCCGTGTGAGGTATATATTACATACACAGTTCCTCCTTTTGTTACTTCAGTTCAGAAAGGAATAGCTGCCTGATACCTGATTAGTGCGTCTTCACAAGAATGAATTCATGATTGTGCCTCTGCTGAAGGTAGCCCTGCAGAATGAATTGATACGAAGCCACACGTTAATTTGAGAAATATTGCTACAGAAGATCTTAAAATGCTGTTGAATGTAGGTTGCGACATATAATATCTCTTTGTTTTCAGGACATTACATTGTTTAAAGTAGTTGTACGAAGCATTTGTGGTGCAAATATCATATAAAGTATGGAATAAATGCCCTTGTATGGATAACTTGTCTTCTGAGTGATCATTCTGTCATTGAACAAAGGTTATGGAATCTGAAGTTGAAGGAAGGAAAGAGAAAAACGTGGTATATATCCCCAAATGCAGGTAATTCAATCATCATCAAGAAATTGTTTCACAAGTTTGACTAGGAGGAACATTTGTAAAGAAAATTTCTTCAACTGCTGGGTATCACGTAACATATCGGTCAAACGGTTTCCTACTCCCAGCTCTTCATAGGCAGACTACAAAAATTTTCAGGTCTATAAGTAGTAACCTGATGATTTGCAATTAACATATGACTCTGCAGCCATCAATTAGTTCTGAATGAATTGTTGCAGTATGTCAACTCTTACTCAAGTTAATCCACTCATGAAAAACAAATTGAAGAACTATGGCTGGGACACAAGTTGTATTGTCAACGTCGTAATTGTGGCAACTGACAATCCTACTCACACTCCACAAGCTGCTTATTATCAATTCTACCCACGTTAGACAAACTACCATGATTAACACTGGGTTAAGAAAAAAATAGAATTTTAAGTGCAGATATATCAATAAATACTGAAGAACCACCTTAAAAATACATAAATACTGAAGAACAGGATTTCTACTGGTGTTGCTGTTTTTCTTTTCTTTTTTTTTAATCTCCAATCCTATTTGTGGCAGATTTCTGGAAAGTACAAATTGTGTTGGTATGTGCACAAACCTTTGCAAGATTCCATGCCAGAAGTTCATCCAAGATTCACTTGGCATGAAGGTCTACATGTCTCCCAGTAAGCTTCCTCTGCTCTGATTCATCAGGAAAGCCTGAATATCAGTGGGTAAACACAACAACTTAAATATAATTTGGTAGAATAAGGTAAAAATAATCAAACTCCATTCCTTTGCATAAAGTTGGCAAGAAACTAATAATGGATTGTCATATCAACATCATCTCAAAGTTGAAAATTTGGGTTGAAACCTGGTGTGCCTCTAACTTCTCAGGACATTGACCTAATTTTACTATAGCGGATCATTATACGGACTGCCGCTAAAACATAAGTGAACTCAAAATATCAACTTTCGACTTTAAGCAGAATTAGTGAAGTAAAGTTGAATCAATGTAATAGGTCCATAGACCACCAACGGTTTAGAGCTATGAGAAATACAGAACTTAATTTGACATATTAATTTCATGCCACCTCTACCTACCGACAATCTGAGGACTGTGCCCTTTTTGGGTATGGGAACCACTGGCCACCATTCAGGAAACAGAAAGGGCTGAAGTGGGGCCAACATGTTTGCAGGTTACTACCTCCGTTTTTTAATTTATAACGTCGATGACTTTTTAGATATATATGATCATTCGTCTTATTCAAAAAAAAATGCAATTATCATTTATTTTATTGTGACTTAATTTATCATCAAATGTTCTTTAAGCATGACTTAAATTTTTTTTATATTTGCACAATAATTTTGAATAAGATGAATGGTCAAATGTTTGTCAAAAAGTCAACAACGTCATACATTAAAAAACGGAGGGAGTACAAAGCAATCACAAGCAAGTTGTACAGAAGAAGAAAGTGCTAGCCCCTACTTGTCATTCTTAGTGGGAAACGGCAAATTGCATGGACAAGTTGTGGAACAGTAGTGTGAAATCCAGAAATCCACAATGTAGTGCTGTGAAAATACAGTACAAGCACCTTCACATGGCAGTGACGAGCCAGCATGTCTCGATAATCAGAGCAATATGGTTCAATTTCACTTATGAGAAGCACTAGTTTTGAATGTTAGTCTTCTATGGTGATACACTGAATATTTCAACATCAATCATTTTTTCTAGGTCAAAAGATACTGATATTTTATGACATCACCCCACAGAATCAGATATCAACCTCTGTTGGAATTTGCAAGGCTCTCTTTCCTTTTTATCCTGTCGAGTGGTTAGATCTAGGAGTAAGCAATGAATGTTATTTACTGCCAGTGCTACTAAATAGTCCTATACAGCTTATTTCCAATTAAGCAAGAAGGTTTTTTCCAATACTAGGTCAATAAAAACGAGAATAAAGCAGAGTCCCTAAAGATAATAGTGAATGTATCAAGAGAAATGTAACATAATTACTTACATCAACTATATATTTATTCTTCAGATTTTGAAGACATGAGCTGTGAGATGATATTTGGACAGCAACCTCCTGAAGATGACCCTGCATTGAAGCAGCCATGCTTCCGGACAAAATGTAAGGAATTCCGCACTGAGGCACCTTCATGGATTCAATCAACACTCCTCTATATTCATACTTTTTACTTCGTGTATATGCAGGCGTCGCAAAGCAGAATCATGGTGTGAATTGCTCCATCTGATCTGAAAGAAATTATCAATAGATAGATTTCAAATCAGTAAAATGCCTTAAGCTCCATTTCCTTTATTCCTTTGGAAAAAAAATTAGCACCATCATTGTTTTTGCCCACAACACCAGCATGTTTGGAACATACACTCTTCATTGTAATCCAAAAGTAATCTAAGAGGAAATGAAAGGCCCAACAGTAACCATTTTAAGGTA</cdnaseq>
Protein Sequence	<aaseq>METTTLVLLLPHGGAGGVRPAAAATAKRSYVMRRCCSTVRAVMARPQEAPASAPAKKTETAAMMSTVQTETAAAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQALERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPEDDPALKQPCFRTKCVAKQNHGVNCSI</aaseq>
Gene Sequence	<dnaseqindica>1..304#1861..2000#2217..2309#2670..2731#3293..3399#4756..4845#4929..4969#ATTCCCACCACAACCAAGATGCCCTCTCCATGCCATTTGGTCTCTTCTCTCTCCCTCCTTGCAAATTGCATGACCCTCTCTCTCTCTCTCCACTTCTCTCTATAAACCTTCCTCTCTCCCATAACTTCTTTCCATTTTCAACCTACAAATATACTAATCTCTCTCTAGCTAGTCTTCACCTACAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCATGGAGACCACCACGCTTGTGCTGCTTCTTCCTCATGGCGGCGCCGGCGGCGTACGGCCGGCGGCAGCGGCAACGGCGAAGCGAAGCTACGTGATGAGGAGGTGTTGCTCGACGGTGAGGGCGGTCATGGCGAGGCCGCAAGAGGCGCCGGCGTCGGCGCCGGCCAAGAAGACGGAGACGGCGGCGATGATGTCGACGGTGCAGACGGAGACGGCGGCGGCGCCGCCGGCGACGGTGTACCGGGACAGCTGGTTCGACAAGCTCGCCATTGGTTACCTGTCCAGGAACCTTCAAGAAGCTTCTGGTTCGTGCCTATAATCTACTGAATATATCTGATGTATTGTGTGCATGTACATGCAATCTTTCGGATGAATTAATGAATGATCAAATCAACCATGCATGCATATATGTGTGTATTTATGATGTTTTTGCCATGGCTGATTTGATTTTTTGAGTGGAAGAAATAACATATATATGGTGAATAAACCAATGTTCTTCAGTTATTTTTGTGACAATTAAATTATATGTGTATTCTACGATCATCCTTTTCTTCTTATCGTTTGGTTATTAATACCCTTCTCGTCCATGTGGAACATAAATTCTTTGATTTTTTTTATTACATGTTGCTTTGGCCAGCGTGGGTGTACTTGCAATTGCATGCTTTCTGTCATATTATAATTGAATTAGCTCTTGATTTTTACATGCAAAAGAATAATTTATTTATTGAGTTGCTGATGATCACGTAAGCCTATGCATGTGAGATGTGATGTGTTCTCCGGCCTAATTAAGTATCGACGTACTATATATATGCACGTACATCAAGTACGGCCGTATTTCATAATTTGATTATTGTTACTGTACCAGACAAACCTAGTGCTTAATTATTGACATATACCCAACATCGATTAAAGTCAGTGTGACACAATTGGCAGAAGAAGATAACAAAAATCATAGAAGACATATATACCACAACAGTAAAAATTACAGTGCAAAAAGGGATACCAGAAGCTTCTTAATTAGGTGAATGGTCACATTTAGTAGTAGTAATACACAGTAATAACACATTATTTGACGAATAAATTAAGCGGAAGTGCATGATCAGCAGCATATAAGCAAGCAACTACGAGAGGTCAACCGTTGATCCGTGAAAATGTGAGGTTCACATTCTAGGAATCTTTTAGTGCTTGAATCTAATCCTTTCGTTTGTTTTCCCGTGGACTTTTTACTTTGATCGAATAAACTTATCTGAATAATGCGAAGGTCAAACATCACATGTACGTGCCCGGTTCTTCTTCTAGAAATTCAGCCGCTTACTATAATCGATGCTGGTTTTTGAATTTTTTTAAAGACATTTATTTTGGAACAACTTAAAAAAAGTGCACAAACCAGGGTTTACCAAACCGTTTGAAGTGAGGTTACTGCCACTCCATGGTTTGGCAATTATCACGAGGTGCGTCGTGGTTCCAAAAACTAATAAGGTTATCGTATGTGATAACCGCGATAACTGACGGTTTTGTAAACCCTGCACAGACAACATAATTATATTTACAGGACTGTTTGGACTTCATTAACACTGTAGCATCCACTACATCCGTCACCTCCTTGGCTCTCTTACATGTTTAGCAAAGTCAATCATACAGACAAACCAGAAAAGTTCCCAACAGACACAGAATGCTACTTTTCTAGTATGGGTAATTAAGCATATAAGAGAAACTTGAAAGCATACAGGAAACCGATGTTCACTAGTCCTGAGTTTTTTTTTGGTGTTGTTCAGGTGCAAATTAACTGCAAAGGTTGATTCCCTGAAAGGAACTTGTAGCACTTGCTGACTGACACGAACATGCATGCAATGTAATGCAGGGCTAAAGAATGAAAAGGATGGCTACGAGAGCCTGATAGATGCCGCCCTAGCCATCTCAAGAATCTTCAGTCTGGATAAACAAAGCGAGATTGTGACCCAAGCTCTTGAAAGAGCACTTCCAAGCTACATCCTCACAATGGTAAGTACCATAATCCATGACAATTGGCAATCATGTATGAATTATTGAAATTTAAAAAACCTAAAACTTTTTTTTATTTGAACAGGAAGTAAAATTCAGCATTTATTCTCTCTTTTTTTTTTGAGGAAAATACAGAACTTCTGTAAAGGGAGGAAACTTTAAAAGTTCTCTTCTTTTATTTCTTACCTCAACTTTGATTGAGAATTCTGTCGGCAGATCAAGGTGATGATGCCACCTTCAAGATTTTCCAGGGAGTACTTTGCTGCATTCACCACGATATTTTTTCCTTGGTTGGTTGGGCCGTGTGAGGTATATATTACATACACAGTTCCTCCTTTTGTTACTTCAGTTCAGAAAGGAATAGCTGCCTGATACCTGATTAGTGCGTCTTCACAAGAATGAATTCATGATTGTGCCTCTGCTGAAGGTAGCCCTGCAGAATGAATTGATACGAAGCCACACGTTAATTTGAGAAATATTGCTACAGAAGATCTTAAAATGCTGTTGAATGTAGGTTGCGACATATAATATCTCTTTGTTTTCAGGACATTACATTGTTTAAAGTAGTTGTACGAAGCATTTGTGGTGCAAATATCATATAAAGTATGGAATAAATGCCCTTGTATGGATAACTTGTCTTCTGAGTGATCATTCTGTCATTGAACAAAGGTTATGGAATCTGAAGTTGAAGGAAGGAAAGAGAAAAACGTGGTATATATCCCCAAATGCAGGTAATTCAATCATCATCAAGAAATTGTTTCACAAGTTTGACTAGGAGGAACATTTGTAAAGAAAATTTCTTCAACTGCTGGGTATCACGTAACATATCGGTCAAACGGTTTCCTACTCCCAGCTCTTCATAGGCAGACTACAAAAATTTTCAGGTCTATAAGTAGTAACCTGATGATTTGCAATTAACATATGACTCTGCAGCCATCAATTAGTTCTGAATGAATTGTTGCAGTATGTCAACTCTTACTCAAGTTAATCCACTCATGAAAAACAAATTGAAGAACTATGGCTGGGACACAAGTTGTATTGTCAACGTCGTAATTGTGGCAACTGACAATCCTACTCACACTCCACAAGCTGCTTATTATCAATTCTACCCACGTTAGACAAACTACCATGATTAACACTGGGTTAAGAAAAAAATAGAATTTTAAGTGCAGATATATCAATAAATACTGAAGAACCACCTTAAAAATACATAAATACTGAAGAACAGGATTTCTACTGGTGTTGCTGTTTTTCTTTTCTTTTTTTTTAATCTCCAATCCTATTTGTGGCAGATTTCTGGAAAGTACAAATTGTGTTGGTATGTGCACAAACCTTTGCAAGATTCCATGCCAGAAGTTCATCCAAGATTCACTTGGCATGAAGGTCTACATGTCTCCCAGTAAGCTTCCTCTGCTCTGATTCATCAGGAAAGCCTGAATATCAGTGGGTAAACACAACAACTTAAATATAATTTGGTAGAATAAGGTAAAAATAATCAAACTCCATTCCTTTGCATAAAGTTGGCAAGAAACTAATAATGGATTGTCATATCAACATCATCTCAAAGTTGAAAATTTGGGTTGAAACCTGGTGTGCCTCTAACTTCTCAGGACATTGACCTAATTTTACTATAGCGGATCATTATACGGACTGCCGCTAAAACATAAGTGAACTCAAAATATCAACTTTCGACTTTAAGCAGAATTAGTGAAGTAAAGTTGAATCAATGTAATAGGTCCATAGACCACCAACGGTTTAGAGCTATGAGAAATACAGAACTTAATTTGACATATTAATTTCATGCCACCTCTACCTACCGACAATCTGAGGACTGTGCCCTTTTTGGGTATGGGAACCACTGGCCACCATTCAGGAAACAGAAAGGGCTGAAGTGGGGCCAACATGTTTGCAGGTTACTACCTCCGTTTTTTAATTTATAACGTCGATGACTTTTTAGATATATATGATCATTCGTCTTATTCAAAAAAAAATGCAATTATCATTTATTTTATTGTGACTTAATTTATCATCAAATGTTCTTTAAGCATGACTTAAATTTTTTTTATATTTGCACAATAATTTTGAATAAGATGAATGGTCAAATGTTTGTCAAAAAGTCAACAACGTCATACATTAAAAAACGGAGGGAGTACAAAGCAATCACAAGCAAGTTGTACAGAAGAAGAAAGTGCTAGCCCCTACTTGTCATTCTTAGTGGGAAACGGCAAATTGCATGGACAAGTTGTGGAACAGTAGTGTGAAATCCAGAAATCCACAATGTAGTGCTGTGAAAATACAGTACAAGCACCTTCACATGGCAGTGACGAGCCAGCATGTCTCGATAATCAGAGCAATATGGTTCAATTTCACTTATGAGAAGCACTAGTTTTGAATGTTAGTCTTCTATGGTGATACACTGAATATTTCAACATCAATCATTTTTTCTAGGTCAAAAGATACTGATATTTTATGACATCACCCCACAGAATCAGATATCAACCTCTGTTGGAATTTGCAAGGCTCTCTTTCCTTTTTATCCTGTCGAGTGGTTAGATCTAGGAGTAAGCAATGAATGTTATTTACTGCCAGTGCTACTAAATAGTCCTATACAGCTTATTTCCAATTAAGCAAGAAGGTTTTTTCCAATACTAGGTCAATAAAAACGAGAATAAAGCAGAGTCCCTAAAGATAATAGTGAATGTATCAAGAGAAATGTAACATAATTACTTACATCAACTATATATTTATTCTTCAGATTTTGAAGACATGAGCTGTGAGATGATATTTGGACAGCAACCTCCTGAAGATGACCCTGCATTGAAGCAGCCATGCTTCCGGACAAAATGTAAGGAATTCCGCACTGAGGCACCTTCATGGATTCAATCAACACTCCTCTATATTCATACTTTTTACTTCGTGTATATGCAGGCGTCGCAAAGCAGAATCATGGTGTGAATTGCTCCATCTGATCTGAAAGAAATTATCAATAGATAGATTTCAAATCAGTAAAATGCCTTAAGCTCCATTTCCTTTATTCCTTTGGAAAAAAAATTAGCACCATCATTGTTTTTGCCCACAACACCAGCATGTTTGGAACATACACTCTTCATTGTAATCCAAAAGTAATCTAAGAGGAAATGAAAGGCCCAACAGTAACCATTTTAAGGTA</dnaseqindica>
External Link(s)	NCBI Gene:DWARF27, [1]