Os10g0167600

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Annotated Information

Gene:OsCPDP; qUVR-10 Protein： Cyclobutane pyrimidine dimer (CPD) photolyase

Function

Repair of CPDs is essential for plant survival while exposed to UV-B-containing sunlight. Nuclear repair of the UV-B-induced CPDs involves the photoreversal of CPDs, photoreactivation, which is mediated by CPD photolyase that monomerizes the CPDs in DNA by using the energy of near-UV and visible light (300–500 nm).There are two kinds of CPD pthotolyase existed in rice leaves. One is 54kD and another is 56kD protein. The 56-kD native rice CPD photolyase was phosphorylated, which has significantly higher CPD photorepair activity. The native rice CPD photolyase possesses both a pterin-like chromophore and an FAD chromophore. Single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.

Expression

Full-length CPD photolyase is encoded by a single gene, not a splice variant, and is expressed and targeted not only to nuclei but also to chloroplasts and mitochondria. Over-expressing CPD photolyase enhances thein vitro photorepair ability of rice. CPD photolyase-over-expressing plants are significantly more resistant to the growth-impairing effects of UVB radiation than the WT plant.

Evolution

Rice may have evolved a CPD photolyase that functions in chloroplasts, mitochondria and nuclei, and that contains DNA to protect cells from the harmful effects of UV-B radiation. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine, which has higher CPD photolyase activities.

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Labs working on this gene

Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan,

Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan

Applied Genomics Laboratory, Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 Japan,

Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

References

1. Masaaki Takahashi;Mika Teranishi;Hiroyuki Ishida;Junji Kawasaki;Atsuko Takeuchi;Tomoyuki Yamaya;Masao Watanabe;Amane Makino;Jun Hidema

 Cyclobutane pyrimidine dimer (CPD) photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA
 The Plant Journal, 2011, 66(3): 433-442

2. Mika Teranishi;Kentaro Nakamura;Hiroshi Morioka;Kazuo Yamamoto;Jun Hidema

 The Native Cyclobutane Pyrimidine Dimer Photolyase of Rice Is Phosphorylated
 Plant Physiology, 2008, 146(4): 1941-1951

3. Jun Hidema;Taku Taguchi;Taiichi Ono;Mika Teranishi;Kazuo Yamamoto;Tadashi Kumagai

 Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UVB radiation
 The Plant Journal, 2007, 50(1): 70-79

4. Jun Hidema;Mika Teranishi;Yutaka Iwamatsu;Tokuhisa Hirouchi;Tadamasa Ueda;Tadashi Sato;Benjamin Burr;Betsy M. Sutherland;Kazuo Yamamoto;Tadashi Kumagai

 Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice
 The Plant Journal, 2005, 43(1): 57-67

5. Tadamasa Ueda;Tadashi Sato;Jun Hidema;Tokuhisa Hirouchi;Kazuo Yamamoto;Tadashi Kumagai;Masahiro Yano

 qUVR-10, a Major Quantitative Trait Locus for Ultraviolet-B Resistance in Rice, Encodes Cyclobutane Pyrimidine Dimer Photolyase
 Genetics, 2005, 171(4): 1941-1950

6. Mika Teranishi;Yutaka Iwamatsu;Jun Hidema;Tadashi Kumagai

 Ultraviolet-B Sensitivities in Japanese Lowland Rice Cultivars: Cyclobutane Pyrimidine Dimer Photolyase Activity and Gene Mutation
 Plant and Cell Physiology, 2004, 45(12): 1848-1856

7. Jun Hidema;Tadashi Kumagai;Betsy M. Sutherland

 UV Radiation–Sensitive Norin 1 Rice Contains Defective Cyclobutane Pyrimidine Dimer Photolyase
 The Plant Cell, 2000, 12(9): 1569-1578

Structured Information

Gene Name	Os10g0167600
Description	Similar to CPD photolyase
Version	NM_001189112.1 GI:297727354 GeneID:9272017
Length	3900 bp
Definition	Oryza sativa Japonica Group Os10g0167600, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 10
Location	Chromosome 10:4343782..4347681
Sequence Coding Region	4343990..4344169,4344287..4344358,4344993..4345074,4345697..4345755,4345844..4345880 ,4346001..4346035
Expression	GEO Profiles:Os10g0167600
Genome Context	<gbrowseImage1> name=NC_008403:4343782..4347681 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008403:4343782..4347681 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgctgctgataaaagagagcatatctatacgtgagagggaacagcttgagaatgccaaaacacatgatcctttgtggaatgcatcgcagttggagatggttcaccatggaaaaatgcatggattcatgagaatgtactgggccaaaaagattctagaatggactagtggaccagaagaagcactttcaactgcaatttatttaaatgacaagtatgagatagatggcagggaccccagtggttacgtcggatgtatgtggtccatatgtggcctccatgatcagggttggaaggagcgtccagtatttggaaagatacgttacatgaattacgctggctgcaagagaaaattcgatgttgacgcttacatttcttatgtcaagagattagctggtcaatccaagaagaggaacgctgaggagtctccaaatcctgtagtcaagctttccaagtctcagcactaa</cdnaseq>
Protein Sequence	<aaseq>MLLIKESISIREREQLENAKTHDPLWNASQLEMVHHGKMHGFMR MYWAKKILEWTSGPEEALSTAIYLNDKYEIDGRDPSGYVGCMWSICGLHDQGWKERPV FGKIRYMNYAGCKRKFDVDAYISYVKRLAGQSKKRNAEESPNPVVKLSKSQH</aaseq>
Gene Sequence	<dnaseqindica>3513..3692#3324..3395#2608..2689#1927..1985#1802..1838#1647..1681#aattcacacccacaaacgcacgcccgcacgcgcgcgcgcgcgctcagagacctccgtcgccgcccgccgcacgccggcgacccaccgcgccgcacgatgccgccgacctcagtgagcccaccaagaacagcgccggggccggcgaacccgagcccggcacacccgtcccgcgtgcgggtgatccacccgggcggcgggaagcccggtgggccggtggtgtactggatgctgcgggaccagcggctcgccgacaactgggcgctcctccacgccgccggcctcgccgccgcctccgcgtccccgctcgccgtcgcgttcgcgctgttcccgaggcccttcctcctctccgcccgccgccgccagctcgggttcctcctccgcggcctccgccgcctcgccgccgacgccgccgcccgccacctccccttcttcctcttcaccggtacgcgcccttttctttttctttttctttttctttttcttttgtctccatccgtgacgatgacatgtgggccaccccgtcttgcgaaatgtgacgtggctttttctctggctcgctgacaggtgggcccgcggagatcccggcgctggtgcggcgccttggtgcgtcgacgctggtcgccgacttctcgccgctgcggccggtgagggaggcgctcgacgcggtggtcggcgacctgcggcgggaggcgcccggcgtggccgtgcaccaggtgagcgtggtggcattgctgtttgcatcaatcttccaagaaagttgggaatctttttggggatggataatgaattcgccggagaatttagagattcttgtggaattcttcgaatgcaggtggacgcgcacaacgtggtgcctgtgtggacggcgtcggcgaagatggagtattcagccaagaccttcagaggcaaggtgagcaaggtgatggatgagtaccttgtggagttccctgaattgccggcggtggtgccatgggacagggagcagccggagggggtcgactgggacgcactcatcgccagggtttgcaggtgcggatgataattctaaggtgtattgggtggtaattttgtttgtgtagatatggatgactattgattcttggttgtttgagcagtgaggcggagaatgtgccggagattgactggtgtgagcctggagaggaagcagccatagaggcgcttctcggcagcaaggatggattcctgacgaagaggatcaagagctatgaaactgaccggaatgatcccacgaaaccacgggcattgtctgggctttcaccataccttcattttgggcacatttcggcacagcggtgtgcgctcgaggcaaagaaatgccgacatcttagtcccaaggtgctgttctttaactgatttgttatgattaaaagagttatgatcctcttgcaatgtaataaattgttagatggcattgtttttctttaccgtgaactggctagaattcttagtgatgttgtttacctaatagatgtttgaagtaacatgtgttctgaaacagtccgtcgatgctttcttggaggaattggttgtaaggagggaattagctgacaacttctgctattaccaacctcaatatgattcgctgtctggtgcatgggaatgggcaaggaagacactgatggatcatgctgctgataaaagagagcatatctatacgtgagtaagttgccctttataatttattgtcacaattttaaccaaattatcttcctgacgatgttaaggtaaaagatcttcgctctaattggttatttgttaattgtcattttgttttggctaggagggaacagcttgagaatgccaaaacacatgatcctgtaagtgctattggacgtggtgtactctgttgctgatattgaagttactctaacacactgagttattttttttctaaatgattatcagttgtggaatgcatcgcagttggagatggttcaccatggaaaaatgcatggattcatgaggtaatatatcatgaccagactcttacaaaatcacgcaattataaaatgtttattgaacagttttttttttatgaaactgcttgttatatttcttctgtacccatggcacattctagttagttgcccataacctattggcccacttgttgacaacatatggttattttacccatcatactgatggtgtggtgctaacctaaaggcattggattgcccccaccccatctttgctgacataactgattagtttgctttgttggatagatgggaattttctgacgttgtggtttactttatcctatgtcagaaaatatgaagttatttgaaaaaaagcattccttaaaaaattagttttctgtagagtttgtgtagttccaataaatttatgatgatctatgtgatatgtctggtagcaagcctttggtgcacataattctctttgattctgggcaaaccttttacactatttttgttcaacttttgttttggatctggaaagaaagaaaagtaagttgttttggcgaacatcaaagaatgatgttatatctaattgattcttagttctgaaggtaacagtgcctgtaaggactttttcctttcctaatgtagtagttgtctgtagaatgtactgggccaaaaagattctagaatggactagtggaccagaagaagcactttcaactgcaatttatttaaatgacaaggtaagacctatacacatgtttgggaaatacagttgtgttattttgaatttgtcctttttatagttttgctagttgtagttaaaatagctatatttcaacacatggaacatgataaagaaacatggaacatgataaagaaactgctcacatgaaaaattgtaattgaatagcaacttggaagagtctgcccatgcttaaattagttcctgcgccgataaccgaaaagttggagtcacatttatcaaattaagaaatatataatttgacattcttttcatgtaaggcagaaaattgtatggttggtgctcttttcattatcttatcttcccttaagttaaaaacatcatccttctagggacaaccagacaataaatcagcttcacggtcgcctcggataagctggactatcctttttcttggttacctgtcagaagaaagatacaaaacagtaactggggaaataaattttcaggaatgtgaaattgctcgaagatattttcatagttgaacctacaattctttccccagctggattgttctcgttctttaataggagtagttgtttgcattgaagtacttctacggattttatttatgcttttcaacaaagtcttacaaatcaatcttgtggcagtatgagatagatggcagggaccccagtggttacgtcggatgtatgtggtccatatgtggcctccatgatcaggtcattgtgttatcacttctcacttcacagggaaaaacaattttgcttttcacaagcattagattgttggtgacattctagaagcgagtattccttacaccactgctgttttctcagggttggaaggagcgtccagtatttggaaagatacgttacatgaattacgctggctgcaagagaaaattcgatgttgacgcttacatttcttatgtcaagagattagctggtcaatccaagaagaggaacgctgaggagtctccaaatcctgtagtcaagctttccaagtctcagcactaaatccgactaggaaaatatgaacatttctttgtgtggtataccacgagctcggaaaattgaagaacagtgctaacttgaggtagatcatgtagggtatacactgtagatctgtttgtctgatatccttgtaggcttctaatcattcaaaatttgctagcattgttaagactttaagacatacagtgtcacaccagttgaattcaagtgt</dnaseqindica>
External Link(s)	NCBI Gene:Os10g0167600, RefSeq:Os10g0167600