Difference between revisions of "Os10g0496900"
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'''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure 5.png|200px|left|thumb|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]] | '''OsPORB is not required for light-dependent Chl synthesis during greening of etiolated plants'''[[File:figure 5.png|200px|left|thumb|Figure 1. Abundance of OsPOR transcripts and OsPOR proteins during the greening of etiolated seedlings.]] | ||
| − | First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure | + | First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas ''OsPORB'' expression decreased only slightly (Figure 1a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 1b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in ''OsPORB''. |
| − | Next, to examine whether OsPORA contributes to light-dependent Chl synthesis during leaf greening, Sakuraba et al compared the greening speed of wild-type and fgl seedlings. The visible speed of greening (Figure | + | Next, to examine whether OsPORA contributes to light-dependent Chl synthesis during leaf greening, Sakuraba et al compared the greening speed of wild-type and fgl seedlings. The visible speed of greening (Figure 1c) and the rates of Chl synthesis (Figure 1d) were not significantly altered in fgl, indicating that low levels of OsPORA are sufficient for leaf greening in rice, even in the absence of OsPORB activity in fgl mutants. |
Revision as of 15:01, 9 June 2014
The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions.
Contents
Annotated Information
Function
OsPORB is essential for maintaining light-dependent Chl synthesis throughout leaf development, especially under HL conditions. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. NADPH:protochlorophyllide oxidoreductase (POR) catalyzes photoreduction of protochlorophyllide (Pchlide) to chlorophyllide in chlorophyll (Chl) synthesis, and is required for prolamellar body (PLB) formation in etioplasts.
Rice faded green leaf (fgl) mutants develop yellow/white leaf variegation and necrotic lesions during leaf elongation in field-grown plants. Map-based cloning revealed that FGL encodes OsPORB, one of two rice POR isoforms. In fgl, etiolated seedlings contained smaller PLBs in etioplasts, and lower levels of total and photoactive Pchlide. Under constant or high light (HL) conditions, newly emerging green leaves rapidly turned yellow and formed lesions. Increased levels of non-photoactive Pchlide, which acts as a photosensitizer, may cause reactive oxygen accumulation and lesion formation.
fgl harbors a 1-bp deletion in the coding region of OsPORB, resulting in a frameshift mutation and premature translational termination. Based on the expression analysis of two OsPOR genes and phenotypic characterization of the fgl mutant, Yasuhito Sakurabaet al[1] proposed that OsPORB plays important roles in maintaining a threshold Chl level throughout leaf development, especially under HL field conditions.
Expression
OsPORB expression is regulated in a circadian rhythm in short-day conditions. OsPORA was expressed at high levels in developing leaves and decreased dramatically in fully mature leaves, whereas OsPORB expression was relatively constant throughout leaf development. However, OsPORB expression is rapidly upregulated by HL treatment.
First, Sakuraba et al examined the effect of light on OsPOR expression during greening. When 6–day-old etiolated wild-type seedlings were exposed to light for 24 h, OsPORA expression was rapidly downregulated after illumination, whereas OsPORB expression decreased only slightly (Figure 1a). Consistent with its mRNA levels, OsPORA protein levels were severely reduced after 6 h of light treatment, and were maintained at low levels until 12 h in both wild-type and fgl seedlings (Figure 1b). OsPORB remained relatively constant in the wild type, but OsPORB did not accumulate in fgl because of the frameshift mutation in OsPORB. Next, to examine whether OsPORA contributes to light-dependent Chl synthesis during leaf greening, Sakuraba et al compared the greening speed of wild-type and fgl seedlings. The visible speed of greening (Figure 1c) and the rates of Chl synthesis (Figure 1d) were not significantly altered in fgl, indicating that low levels of OsPORA are sufficient for leaf greening in rice, even in the absence of OsPORB activity in fgl mutants.
Enhanced OsPORB activity is essential for Chl synthesis under HL.
Evolution
Labs working on this gene
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences,Seoul National University, Seoul 151-921, Korea
References
1.Yasuhito Sakuraba;Md Lutfor Rahman;Sung-Hwan Cho;Ye-Sol Kim;Hee-Jong Koh;Soo-Cheul Yoo;Nam-Chon Paek
The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions The Plant Journal, 2013, 74(1): 122-133
2.Qiaosong Yang;Han He;Heying Li;Hua Tian;Jianjun Zhang;Liguang Zhai;Jiandong Chen;Hong Wu;Ganjun Yi;Zheng-Hui He;Xinxiang Peng
NOA1 Functions in a Temperature-Dependent Manner to Regulate Chlorophyll Biosynthesis and Rubisco Formation in Rice PLoS ONE, 2011, 6(5): e20015
Structured Information
| Gene Name |
Os10g0496900 |
|---|---|
| Description |
Similar to NADPH:protochlorophyllide oxidoreductase porB (Fragment) |
| Version |
NM_001071490.1 GI:115482723 GeneID:4349004 |
| Length |
3713 bp |
| Definition |
Oryza sativa Japonica Group Os10g0496900, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:19357502..19361214 |
| Sequence Coding Region |
19359252..19359591,19360224..19360750 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:19357502..19361214 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:19357502..19361214 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggggtgccgcgacttcctcaaggcgtcgcgcgccgccaaggccgccggcatggagaagggcagctacaccatcgtccacctcgacctggcgtcgctcgacagcgtcaggcagttcgtcgccaacgtccggcggctggagatgcccgtcgacgtggtggtgtgcaacgccgccgtgtaccagcccaccgccaagcagccgagcttcaccgccgacggcttcgagatgagcgtcggcgtcaaccacctcgggcacttcctcctcgcccgcgagctcctcgccgacctcacctcctccgactacccctccaagcgcctcatcatcgtcggctccatcaccgggaacacgaacacgctggcggggaacgtgccgccgaaggcgaacctgggggacctccgggggctcgcctcgggcctcgacggcgtgtcgagctccgccatgatcgacggcggcgagttcgacggcgccaaggcctacaaggacagcaaggtgtgcaacatgctgacgatgcaggagttccaccgccggtaccacggcgagaccggggtgacgttcgcgtcgctctaccccgggtgcatcgccaccacgggcctcttccgggagcacgtcccgctgttccgcctcctcttcccgcccttccagaagtacatcaccaagggctacgtctccgaggaggaggccggcaagcggctggcccaggtcgtcagtgaccccagcctcaccaagtccggggtgtactggagctggaacaacaactcggcctcgttcgagaaccagctctccgaggaggcctccgatccggagaaggccaagaaggtctgggagctcagcgagaagctcgtcggcttggccgatcacgatcagtga</cdnaseq> |
| Protein Sequence |
<aaseq>MGCRDFLKASRAAKAAGMEKGSYTIVHLDLASLDSVRQFVANVR RLEMPVDVVVCNAAVYQPTAKQPSFTADGFEMSVGVNHLGHFLLARELLADLTSSDYP SKRLIIVGSITGNTNTLAGNVPPKANLGDLRGLASGLDGVSSSAMIDGGEFDGAKAYK DSKVCNMLTMQEFHRRYHGETGVTFASLYPGCIATTGLFREHVPLFRLLFPPFQKYIT KGYVSEEEAGKRLAQVVSDPSLTKSGVYWSWNNNSASFENQLSEEASDPEKAKKVWEL SEKLVGLADHDQ</aaseq> |
| Gene Sequence |
<dnaseqindica>1751..2090#2723..3249#agcttagaaccccaaccccccaaagcctcactcacttcgctgcagaggaaaaaaaagagagaaaaatctccgatggctctccaggcggccaccaccacctccttcctcccctccgcgctctccgcccgcaaggaggtgagagctctaagctcggggattcagccatggaggcatttcagagttcagactagttcagagttttgcttcgtgttcatggcggcgattggttaaatggttttttttttgtttttgggttggtttttgggggtgtagggagcggtgaaggactcggcgttcttgggcgttcgtctcggcgacgggctcaagctggagaccagtgctctcggccttcgcaccaaggtagtaactgtaataatgttgttacagcactctgcttgctctgtgctgatgctctgtttgttagtgctaatattagtacttactactaactggcgagtagtacaagtaattggctagttcgttcagtgaattgccaggttttcgtttctagacttcacgattattagtttcagactttcagttatgttggaggagcttaccactgtggctctgtggtttgctctgtcagattaggagcacccgtggcgttgcaaatggttatgcctgtgtttggcattaatcaactggctaatgagattatatcatgatgattctctcatcgttttagctatgacaatgtcaggggcttgttttgttttcacaggtgtgcagctttgctaattgctagtaacatcagtgtgtgccgccattgttagtgcaaccaagtagcctttgtgtctggttaatttgcaacttcaggttgacatttggacttgctgagtgagtgtatctagcattgcaaactgatgtgaattttgacgaacttttagtgcaagattgcaaggtttgcttgatgtacttagaaatggtagttgctcacaactctgacagcgccacaaaaattgtattatacttgattagcctggcattttgacttcctattgatctggattttatgtcagtgttagtttgagtatatatagcattgcaaagtgatgcgaatttgggtaacttttagtgcaagattgcaaggtttacttgctgtacttagaaatagttgctcaaaactctgacatcgccacacccccaaaaaattgtactactacttaattagctttggcatttcaagttcatattgatgtggatcgatttcataaacttgattttactgcaagactgtcaagtttgctccctgtaatttcataaacttggttccgcatgtaaactagacgaatttattaagcctaattaatccatcattagtaaatatttattgtagcatcacattattaaatcatagcataattagattcaaaagatttgtctcacaatttacatataaactgtgcaattatttttttccccacatttaatattcttgcatgttcaaacatttgatgtgatgtttttggccaaaaaattttttatataaaaacaaaggcctagtttgttcatatatcttgacattgttgccacgaaaactgaaactctcctgcagagggtgagcacgtcgtcggtggccatccgcgcgcaggcgtcggcggcggtgtcgtccccgacggtgacgccggcgtcgccgtcgggcaagcagacgctgcgcaagggcacggcggtcatcaccggcgcgtcgtccgggcttggcctcgcgacggcgaaggcgctggcggagacgggcaggtggcacgtcgtcatggggtgccgcgacttcctcaaggcgtcgcgcgccgccaaggccgccggcatggagaagggcagctacaccatcgtccacctcgacctggcgtcgctcgacagcgtcaggcagttcgtcgccaacgtccggcggctggagatgcccgtcgacgtggtggtgtgcaacgccgccgtgtaccagcccaccgccaagcagccgagcttcaccgccgacggcttcgagatgagcgtcggcgtcaaccacctcgggcacttcctcctcgcccgcgagctcctcgccgacctcacctcctccgactacccctccaagcgcctcatcatcgtcggctccatcaccggtaatgacaacctttcttcctcaccagaattaggctgttgtgttctaatgtcaaagcttccaacttctactattttgtagttctccacgtacacaattactacaattactgaactgctaaaaattgcatgttttataaaaaaaattataggaagttgttgtgattaatccaatttttaagtttttctttttcatcgggaagattgaaagagaccttctgaatgtattaagaatgagaaaaagttacaagaaaaacataataggaagttgtcgaggatgatgcgcaccagcgtgtgcgttcaagaaccacgagctaaccacacaagcacaaacctctaaaagtgaaaacttcttcttagataatacttaattaatggattatttttctgtgcatggagggcacaaccttatactgctttctaaaatgggtatgataattatttgatagtataaacgaatgaatcaagtattacaaagtcgataagctgatttttttttaaaaaaaaactttataagtgcagttgtaacttttttttaaaaaaaatatcatatcatttgaaagcatatcggcaacattctattgtaacatgttactataagaacatcgttctaatttcgatacgatgaatgcagggaacacgaacacgctggcggggaacgtgccgccgaaggcgaacctgggggacctccgggggctcgcctcgggcctcgacggcgtgtcgagctccgccatgatcgacggcggcgagttcgacggcgccaaggcctacaaggacagcaaggtgtgcaacatgctgacgatgcaggagttccaccgccggtaccacggcgagaccggggtgacgttcgcgtcgctctaccccgggtgcatcgccaccacgggcctcttccgggagcacgtcccgctgttccgcctcctcttcccgcccttccagaagtacatcaccaagggctacgtctccgaggaggaggccggcaagcggctggcccaggtcgtcagtgaccccagcctcaccaagtccggggtgtactggagctggaacaacaactcggcctcgttcgagaaccagctctccgaggaggcctccgatccggagaaggccaagaaggtctgggagctcagcgagaagctcgtcggcttggccgatcacgatcagtgagtgagagtgatgtgctattgattttcgtctaggattttgctgtgctcttcttcttcttctcctctctaccaagaaagatcgatggaggagaatttgtaggacgcgtttctcacgaattacttagctgttaatgatcagcttgatgtgtacgatatgatggtgcagagtgaaagttgtgttgttcactggtggatcatgggatgggaatatgggattgttgtaagatgtaactcaagtgttttcttttttgggattacttttggtaataagagcttgggtgatcgaaaactacagatggtttttcttttaagttgtatgatctctgtagagtttttgagtaatttgtagttttgtaccctatcaaagatcatctctagctgcctctgagctctccaactctatatgtccatctctagtatatatgtcccatatttctgactgaaaattttcaagtcggttggttc</dnaseqindica> |
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