Difference between revisions of "Os06g0643300"
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===Expression=== | ===Expression=== | ||
| − | + | 1.In wild-type seeds, the accumulation of RISBZ1 was very high until 14 days after flowering (DAF), then radically declined to low or undetectable levels (Figure 1c). RPBF accumulation was detectable throughout the endosperm development, and reached a maximum at 14 DAF (Figure 1c). | |
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| + | 2. In wild-type seed,RISBZ1 was highly expressed until 7 and 14 DAF (Figure 1d).RPBF expression reached a maximum at 14 DAF, declining afterwards to approximately the same level as found at 7 DAF (Figure 1d). | ||
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| + | 3. In both KD-RISBZ1 and KD-RPBF seeds, glutelin GluA was slightly decreased, and the level of GluB was comparable with that of the wild type. The GluD level was enhanced in KD-RISBZ1 and completely lost in KD-RPBF.(figure2) | ||
===Localization=== | ===Localization=== | ||
Revision as of 09:26, 10 June 2014
The combination of RISBZ1 and RPBF play critical roles in global processes, including SSP gene expression, during seed development.
Contents
Annotated Information
Function
Please input function information here.
Expression
1.In wild-type seeds, the accumulation of RISBZ1 was very high until 14 days after flowering (DAF), then radically declined to low or undetectable levels (Figure 1c). RPBF accumulation was detectable throughout the endosperm development, and reached a maximum at 14 DAF (Figure 1c).
2. In wild-type seed,RISBZ1 was highly expressed until 7 and 14 DAF (Figure 1d).RPBF expression reached a maximum at 14 DAF, declining afterwards to approximately the same level as found at 7 DAF (Figure 1d).
3. In both KD-RISBZ1 and KD-RPBF seeds, glutelin GluA was slightly decreased, and the level of GluB was comparable with that of the wild type. The GluD level was enhanced in KD-RISBZ1 and completely lost in KD-RPBF.(figure2)
Localization
RISBZ1 and RPBF are localized in the nucleus, and interact in rice protoplasts. When immunofluorescently detected with anti-HA and 4¢,6-diamidino-2-phenylindole (DAPI) staining, it was shown that bothRISBZ1andRPBF were localized in the nucleus. (Figure 5a)
Labs working on this gene
1.Transgenic Crop Research and Development Centre, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
2 Graduate School of Science and Technology, Chiba University, Matsudo, Chiba 271-8510, Japan
References
1.Taiji Kawakatsu , Masayuki P. Yamamoto, Satoru M. Touno et al(2009). Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, The Plant Journal(2009)59, 908–920.
2. Adachi, T., Izumi, H., Yamada, T., Tanaka, K., Takeuchi, S., Nakamura, R. and Matsuda, T.(1993) Gene structure and expression of rice seed allergenic proteins belonging to the alphaamylase/trypsin inhibitor family.Plant Mol. Biol.21, 239–248.
Structured Information
| Gene Name |
Os06g0643300 |
|---|---|
| Description |
Development protein-like protein |
| Version |
NM_001064712.1 GI:115469155 GeneID:4341638 |
| Length |
3154 bp |
| Definition |
Oryza sativa Japonica Group Os06g0643300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 6:27092503..27095656 |
| Sequence Coding Region |
27092681..27093298 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008399:27092503..27095656 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008399:27092503..27095656 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggggaacccggagaagctgatgacccagatcttcgacctcaagttcacctccaagtcgctgcagcggcaggcgcgcaagtgcgagaaggaggagaaggagcagaagctcaaggtgaagaaggcgatcgagaaggggaacatggacggcgcgcggatctacgccgagaacgccatccgcaagcgcaccgagcacatgaactacctccgcctcgcctcccgcctcgacgccgtcgtcgcgcgcctcgacacccaggcgaagatgcaggtcatcggcaagtccatggccaacatcgtcaagtcgctcgactccgcgctcgccacggggaacctgcagaagatgtccgagaccatggacaacttcgagcgccagttcgtcaacatggaggtccaggccgagttcatggagggcgccatggccggctccacctcgctctccacgcccgagaccgaggtcaacagcctcatgcagcaagtcgccgacgactacggccttgaggtctccgttgggttgccgcaggccgccgctcacgccataccagctgccaaggagaaggagaaggctgttgatgaagacgacctctcccgccgcctggctgagcttaaggcccgcggctga</cdnaseq> |
| Protein Sequence |
<aaseq>MGNPEKLMTQIFDLKFTSKSLQRQARKCEKEEKEQKLKVKKAIE KGNMDGARIYAENAIRKRTEHMNYLRLASRLDAVVARLDTQAKMQVIGKSMANIVKSL DSALATGNLQKMSETMDNFERQFVNMEVQAEFMEGAMAGSTSLSTPETEVNSLMQQVA DDYGLEVSVGLPQAAAHAIPAAKEKEKAVDEDDLSRRLAELKARG</aaseq> |
| Gene Sequence |
<dnaseqindica>179..796#gaatcaaatcaaacggaaccgaatcccatctccccaataataaaaatccccaattcgcgtctcgtctcctcgcaaatacgacgcgttcccaaatcccccaatccgatctgcctttcctcttcctcctcgtcaaaccctagccaaatccacgatcggggctagctcgtcggcggcggcgatggggaacccggagaagctgatgacccagatcttcgacctcaagttcacctccaagtcgctgcagcggcaggcgcgcaagtgcgagaaggaggagaaggagcagaagctcaaggtgaagaaggcgatcgagaaggggaacatggacggcgcgcggatctacgccgagaacgccatccgcaagcgcaccgagcacatgaactacctccgcctcgcctcccgcctcgacgccgtcgtcgcgcgcctcgacacccaggcgaagatgcaggtcatcggcaagtccatggccaacatcgtcaagtcgctcgactccgcgctcgccacggggaacctgcagaagatgtccgagaccatggacaacttcgagcgccagttcgtcaacatggaggtccaggccgagttcatggagggcgccatggccggctccacctcgctctccacgcccgagaccgaggtcaacagcctcatgcagcaagtcgccgacgactacggccttgaggtctccgttgggttgccgcaggccgccgctcacgccataccagctgccaaggagaaggagaaggctgttgatgaagacgacctctcccgccgcctggctgagcttaaggcccgcggctgagggatttcacttcaggtgataaacatagccacccctagcatcatacatgaatacttaccttctgttgcacttgtgaaatattgttttagtttttatcaatttgatggagaatgtgaaggtggttatgtggtgtgtatctagtatctacaagaccacgctgtttccttgagttatactcctactttggcctttctgaatgcagctgcgcaccgaaaaataatggctatgctgctatggtttaagatttgtggaaacataagactaaccatacctcacatcttacctgtcaaatggcgagggttactgaggtgttgcatattcatgtcatgctccctgtgctgaagacatcgtcattgcataacatggatgccaataaaacaatgagtcatgctgttatcactgacctaacaatcaggccattcggctacctgggctgtggctgcggctgctgctgcccgctgtttccttgctgcttgcagcactgttgctgttgtctgtggcgcagatgcagcccaggcagccaaacagccttaatttcaccaatggaaatttctgtgtattccaatatcaaatagcatcatacatgcggcaaatcatctcttctgttggctgattacttgggaggtatgtagtgttgtgaattgtgataggacataaaatgactttcaggatttttaacagctaacatgcggtagtcctatattatgacttactacggaattgatattttgataatgatttactcttcttgtactacctaaaaagtgaggaggatagcaaataacaaggataatattattctgctttaacttaggagcacccttaacagcatgtatgaagtgagaccagtgatcattggtgtgctcagatgatatcatgataacacctattcattttgtgtaaattgtattgttttacctgctgaaacataaaagttgtctttttactcttgaatgttcagcatgttttggatgtaaaattatgtttaaactctctgggctagctattagattaaaaggtttttggtgtttttatgttgccttctcaattgtatatttgacagttaaatcagtacgcaagcagttttctgcaccttacatgccaggaagccactcacagaatattggtagtacaaaatgtttatttaatcctactatcagtttgtttatttaatcctactcacagaatgttcatttgttaatgtaagtagcacaagctcttcaccgaatatataaagtaacatttttcatgatgtattttttatgttgagttcctcagtgatgaaatctcatctccatttatttgtaatttgattactattgttacccttgtttgtgttgaaagggctggctttcataacatcctgctgtggtgccctttgtagttgtgtcgaccttccctttatgtgaaacaccacccccttagtgctacatcttggagtgactgcattttctggtgattttacttccaaacaccatataaggctcaacccaaccacaccaaaatattggtaatggcaaagcttttataggctcatttggtctgttgcaaaaattcacaatgccaatactttgggaaggctagacaaaaggttaaagtatctttgccaaaaaaaatttggcatcaaaccatcttaccaaaattttgacaaggaggttaacaatggctacaagccctaacacttggacgaacgttactaaaactttgttgcatttatcaggacatagtgaactttgttgcatttatcgagacagtgtgtatagagtgacttgcacgcataccttttctctatgcgatgcagtctttcaaggccaatttgtattttcatgcaaaatcatagaagggtatacaaatgtcttcaaactttactgtatcgcattttcacatgcttgctaacttaaagcgtaaatgcacgccacaaatttgagttcactaaatgcattctttaatgcatgcatattacatattcactgcactttccatatatgggaaatggcctatgcctatatgcattgattcattaaacctttttccccgttaactaacttccaccgtatctcattttgcagatatgattctcaagctgctatgagccattgtcacgtcaaaggctactcatatttgttagattcgtaaatactgttatgctaaaatgcgtgtgttgatatcccatatgggatctgtggacttcattaaaaccttgctgtgtgatacaaatccataatatgtgagcttgagaacgtctctgtgtcgctgtatcctaagcctgcccgtttgaacagaaatgttgaagttgtccatcttttcacgggatgttttccttgctttaatgccatgaacaaattcggtagccgcccc</dnaseqindica> |
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