Os02g0274900

The rice OsGMST1 gene is a novel Golgi-localized monosaccharide transporter that may play a role in salt stress tolerance.

Annotated Information

Function

OsGMST1 is a member of the monosaccharide transporter family in rice. It encodes a putative monosaccharide transporter.
Monosaccharide transporters play a pivotal role in the translocation and distribution of monosaccharides throughout the plant.The mobility of sugars between source and sink tissues in plants depends on sugar transport proteins. Hong Cao et al.speculates that this gene is a sugar transporter based on its sequence characteristics and the effect on the sugar content when the gene expression is reduced in rice.It is hypothesized that sugar homeostasis is affected in OsGMST1 knockdown transgenic rice, which putatively results in altered sugar contents, so the seedlings show a NaCl hypersensitive phenotype.

GO assignment(s): GO:0005215, GO:0005351, GO:0006810, GO:0008643, GO:0016020, GO:0016021

Mutation

Figure 1.Salt tolerance test of OsGMST1 antisense transgenic rice plants (from reference ).

• There were no differences in the seed germination rate between WT and the transgenic lines without NaCl stress(Figure 1A). Treatment with 150 mM and 200 mM NaCl,however, reduced the germination rate of AS-L12 and ASL18(Figure 1A, B).
• At 200 mM NaCl, the germination rate of the wild type was 90%, while the germination rates of the AS-L12 and AS-L18 lines were reduced to 40% and 50%,respectively (P <0.01) (Figure 1B), and this difference was maintained over several days (P <0.01) (Figure 1C).
• Reduced expression of OsGMST1 also conferredreduced tolerance to salt stress in young seedlings(Figure 1D).
• The survival rate of AS-L12 (40% and 20% in 150 mM NaCl and 200 mM NaCl, respectively) and ASL18 (50% and 30%) were lower than the WT (70% and 50%)with treatment of 150 mM and 200 mM NaCl (P <0.01)(Figure 1E).
• By contrast, the tolerance of OsGMST1 antisense plants to PEG and cold stress was the same as that of WT plants (Figure 1F, G). Just as for SGB1, increased expression of OsGMST1 had no effect.

Expression

Figure 2. Expression analyses of OsGMST1(from reference ).

OsGMST1 expression is induced by salt treatment and reduced expression confers hypersensitivity to salt stress in rice.

• Transgenic rice plants expressing b-glucoronidase gene driven by the OsGMST1 promoter showed that OsGMST2 was expressed in all developmental stages from germination to flowering(Figure 2A).
• Quantitative PCR confirmed high levels of OsGMST1 mRNA in all the organs tested, such as the shoot, root, culm, panicle, leaf, and leaf sheath before and after heading (Figure 2B), suggesting that OsGMST1 is ubiquitously expressed.
• OsGMST1 is expressed ubiquitously (Figure 2A and B),suggesting that it may be involved in sugar translocation in both source and sink tissues of rice.
• The transcript level of OsGMST1 in seedlings was induced by NaCl treatment but unaffected by low temperature and PEG4000. Salt treatment conferred a sustained increase in the steady-state level of the OsGMST1 mRNA.(Figure 2C)NaCl stress up-regulates the expression of OsGMST1 transcriptionally(Figure 2C)One possible explanation is that NaCl stress stimulates the monosaccharide transport activity of OsGMST1 and, consequently, the activated OsGMST1 upon salt stress import sugars into the Golgi or export them out of the organelle to keep cytosolic sugar at homeostasis.

Subcellular localization

• OsGMST1-GFP was co-expressed with the Golgi marker sialyltransferase (ST) tagged red fluorescence protein (RFP), most of the red and green fluorescence merged. The analysis by Hong Cao et al. suggests that OsGMST1 is localized on the Golgi apparatus .

Homologues

Figure 3. Phylogenic analysis of OsGMST1 homologues from rice and other organisms by MEGA 4.0.(from reference) .

Phylogenetic analysis (Fig. 1B) shows that the putative Arabidopsis paralogues to OsGMST1 are encoded by At1g67300, SGB1, At1g05030, and AtpGlcT and the proteins have 67%, 65%, 44%, and 43% identity to OsGMST1,respectively.These four MST proteins are in the pGlcT subfamily. SGB1 Encodes a Putative Hexose Transporter. In rice(Japonica), OsGMST1, Os09g23110, and Os01g04190(OspGlcT) belong to the pGlcT subfamily with sequence identity approximately 36%.SGB1 is shown here to be a Golgi-localized hexose transporter and acts genetically with AGB1 in early seedling development..

Labs working on this gene

• Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
• Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA
• Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA
• National Research Center for Plant Gene, Beijing 100093, China

Structured Information

Gene Name	Os02g0274900
Description	Major facilitator superfamily protein
Version	NM_001053073.1 GI:115445516 GeneID:4328999
Length	6891 bp
Definition	Oryza sativa Japonica Group Os02g0274900, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:10103586..10110476
Sequence Coding Region	10104106..10104187,10104289..10104369,10104522..10104551,10104627..10104759,10106009..10106108 ,10106212..10106324,10106411..10106504,10107201..10107354,10107463..10107606 ,10107702..10107775,10107849..10107937,10108711..10108825,10109581..10109763
Expression	GEO Profiles:Os02g0274900
Genome Context	<gbrowseImage1> name=NC_008395:10103586..10110476 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:10103586..10110476 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgcggtggaagcttaagtcgtcggcgtacaagcgcgtgccgtccagggacgccgccatggatctcgacgtcgagacgccagcgaaaatggcggacggcggggccccgtcgtggcgcatgtcgctgccgcacgtgtgcgtcgccacgctcacctccttcctcttcggcttcgccgggaacaccctcgccgaaggccttgtggtgagcatctgcctgggtggtgcgtttgttgggtgcctgttcagtggatccatcgctgacggaatcggcaggcgtcgcgcgtttcagctcagtgcgctgcccatgatcatcggcgctgccgtaagcgctctcaccaatagtctggagggtatgcttcttggaagatttttggttggaacagggatgggattaggtccaccagtagcttcactgtacataacagaggtttctcctccttcagtgaggggcacatatgggagctttgttcagattgcaacctgccttggaatcgtagtatctctcctaattggtacacctgtgaaggatattgatagatggtggagggtgtgtttctgggttgcagctgttccagcaactttacaagctcttggaatggagttctgtgctgagagcccccagtggctgtataagtgtggaagaacgactgaagcagagatacaatttgaaaaacttctaggtcctcttcatgttaagtctgctatggcagaactctcccgatctgaaagaggcgatgatggagaaaatgtgaagtattcggaattgttttatggtcgcaactttaatgttgtttttattggcacaacgctctttgctttacaacagttatccggcataaattctgtgttctatttctcatcaactgtgttcagaagtgtgggtgtgccccctaaccttgcgaacatatgcatggggatagcaaatttatcaggctcaattgtagctatgcttctaatggacaaactaggaaggaaagtacttctttcagggagcttccttggcatggcctttgcaatgggacttcaggctgttggagcaaatcgtcaccaccttggttctgcaagcgtatatctttcagttggtggcatgctattgtttgtcttgacattttcgttaggagcaggtccagtgccaggacttcttttgcctgagattttcccaaacaaaatacgggccaaggctatggctctgtgcatgtccgtgcattgggtggtaaatttttttgtcagtttgttgttcttgcggcttctggagcaacttggtccacaagttctgtacacaatgttttcctcagcttgtgtggtagcagcaatatttgtgcggcgccatgtggttgaaacaaagggaaagactttgcaagagatagaagtttcgcttctacaaacacaataa</cdnaseq>
Protein Sequence	<aaseq>MRWKLKSSAYKRVPSRDAAMDLDVETPAKMADGGAPSWRMSLPH VCVATLTSFLFGFAGNTLAEGLVVSICLGGAFVGCLFSGSIADGIGRRRAFQLSALPM IIGAAVSALTNSLEGMLLGRFLVGTGMGLGPPVASLYITEVSPPSVRGTYGSFVQIAT CLGIVVSLLIGTPVKDIDRWWRVCFWVAAVPATLQALGMEFCAESPQWLYKCGRTTEA EIQFEKLLGPLHVKSAMAELSRSERGDDGENVKYSELFYGRNFNVVFIGTTLFALQQL SGINSVFYFSSTVFRSVGVPPNLANICMGIANLSGSIVAMLLMDKLGRKVLLSGSFLG MAFAMGLQAVGANRHHLGSASVYLSVGGMLLFVLTFSLGAGPVPGLLLPEIFPNKIRA KAMALCMSVHWVVNFFVSLLFLRLLEQLGPQVLYTMFSSACVVAAIFVRRHVVETKGK TLQEIEVSLLQTQ</aaseq>
Gene Sequence	<dnaseqindica>521..602#704..784#937..966#1042..1174#2424..2523#2627..2739#2826..2919#3616..3769#3878..4021#4117..4190#4264..4352#5126..5240#5996..6178#aaaggaaccaacagaagcaaaggtggcattcgttcgtcgcacgagagcaccacagcctcacgacacgagctcatcccccgccatctccgctccgctcctctcctctcctctccgtgctacgccaccaaacccactccccccgagatagagagagagagagagagagatccacagcctcacagcatcaaatcacctcccctttctcccagatccaagccgccgccaccaccccctcgccgtcgccgtcgccccctccggccgatctgatgcccgcgcacgcggcggtggtggtcaggtaactttgtaggccgcctcctcctcgattttttgatttttttttcttctcatgtgctaaccacgccacgccaccaccgctattaatacatccccgaggcgagtgtcgaggcatttaccaccagatcgatctcgccttccgctttcccaaatccctagactgacctcctcctgctgctgcttgcgttgcgttgcgttgcgttgtctctttctccgatcagcgccgatgcggtggaagcttaagtcgtcggcgtacaagcgcgtgccgtccagggacgccgccatggatctcgacgtcgagacgccaggtccgtgcacggttttttctctttttttttccctgcgatttttttttgggtggtgagattttgaggttttttttttctgtgctcggtggcggtttctgcagcgaaaatggcggacggcggggccccgtcgtggcgcatgtcgctgccgcacgtgtgcgtcgccacgctcacctccttcctcttcggctaccactccgggtgcgtgccatccattttctcatttcttcttctggttgtggttgttttcccaactcacggtgtggagcgcgtgcgtgtgattggttggtttggtgcagggtcgtgaacgagccgctcgagagcatctccaccgacctcggcttcgccgggaacaccctcgccgaaggtaagtaagcagccacgattcgggtgaaatttgatgttattgggcatgggagcaattgggtgtcctgtgtttcaggccttgtggtgagcatctgcctgggtggtgcgtttgttgggtgcctgttcagtggatccatcgctgacggaatcggcaggcgtcgcgcgtttcagctcagtgcgctgcccatgatcatcggcgctgccgtaaggtaattcgtggtgtcccacttctgttgacggctacttcctctcaatccttagtccatgcgtggtaatttgtggtgtcacacttctgttgatggctacttcctctgaatccttagtccatgtgtggtaatccatggcatcacacttctgttgatggtcacttccactaaatcctagtccatgtgcagtttggtcagtttggaatgtatttaattgccacttgctagcatttcttattttctagctggagttgttggtttcttggtgttaaacaaataaggtcagtggccactggtagcctggtttcttgttggtattgtatactttatttggaaagttgaatgttgggaacaacaaacttactctttcttatgtatcatattgatatattggtggtgtgacaagtagtagcttgatggattcgtccttccatgggattttgaatctccgagttatgtaggagatgaataaaatgtgtggtcctcttaacacagtgcagagagtagtatattgagaatactaggtttgccatcccgccattaggtaaatttatatggtttcttcgactgactgtggagctagtgtaacttcacatgcaaacatgaaagcaatcgaactttacgataaattgactttcactagccacaatagactgaagagtactgaaaattaattacacgcatcttctagaaaaccatggctcaatagtaaatattcttttcttaattgcgctcatgatgtacttagattgattatccttaaaaagggaaaagttctagcctttcaataattcaacactcttattaattgatagctagtgtacgttttggatatagcataccaagtcacttatgggtgttaagtcttggtttatttattggtgctgtagagttgctgataagttgaataataacaacataaaccatatgtgctctgattaccttgaggtgcttttacattgccattgctacatggcatccacaaactaaagttcacttaagtgcattgaatgataatatttaagatgctaacctttttttgaatctttagcacttttatcttctgcatgttatttaataggatccgttttggtagttgcaccaagactgttatttccgaaaatgcaattgtgtaatacttacatctatgagcacctaatacttgagagctgccggaaggaactatgcctgaacttagttattcctatgattctgtttcattgaaccttcctgaagaagaaacattttgcagcgctctcaccaatagtctggagggtatgcttcttggaagatttttggttggaacagggatgggattaggtccaccagtagcttcactgtacataacagaggtatgtaattggtgttatgcacctatgcctcaatctctgattgtctgtatcaaatattaatcttattcatttgtacctttttcttataatcccttggatccaggtttctcctccttcagtgaggggcacatatgggagctttgttcagattgcaacctgccttggaatcgtagtatctctcctaattggtacacctgtgaaggatattgatagatggttagatgctttcttctgtagctctctacatgttttgtgtagttagtaaatctgatgcatctaactttatcctttgctattaacaggtggagggtgtgtttctgggttgcagctgttccagcaactttacaagctcttggaatggagttctgtgctgagagcccccagtggctgtataaggtgtgtttatcttgggggaagctgcttattttctatctatgatcttccaacttaaatcacccttcaggtgtctgattctctataattgtgcttcggcaaatttgatcttaagtgcctttgctagtcgtaaaatgagatactcatatttgaggaccatcttggagtcactttgtcggaaacgacatttgatctcttaaactatatgcaaataccacatgctgatttttccatgaatgtttgaaggtcactgtcgtattaaatcaaacaattaatctgttggttagaacttgcaatctaccatcatcctgaggttttttgtgcttacaattaataatgggttgacacaaacttattgcctattgttacttagatggtaacacgaatgatatctatagaagagtagtccattggcacatattggtatttgcttcagaaagcacacattagattttcggataaatatcgttgatctttagtttcctcaatactgtgcctgttttctgattgtgcaccatgcaaatacagctctgatatgagcaaaacattttattagttttttttctgagaaaaccaattctcaacacagacttatacaactcccatacttctttatattggcaatttgtattgacatatttatatttaacttgtagcacatacattgaaaattattgacgagtcttatcttttattcagtgtggaagaacgactgaagcagagatacaatttgaaaaacttctaggtcctcttcatgttaagtctgctatggcagaactctcccgatctgaaagaggcgatgatggagaaaatgtgaagtattcggaattgttttatggtcgcaactttaatggtacaagctgttatgtgttttccattctgttgtgtatttgcgctaaactataaccttatttatgtttggccttgaagtttgttcattacaagtccctttttgctacagttgtttttattggcacaacgctctttgctttacaacagttatccggcataaattctgtgttctatttctcatcaactgtgttcagaagtgtgggtgtgccccctaaccttgcgaacatatgcatggggatagcaaatttatcaggtgggctcattggagcatcttctctttttttctgttgttctactgttctgttatattgatggttgatggattttgtttgatgtgtttgcttccaggctcaattgtagctatgcttctaatggacaaactaggaaggaaagtacttctttcagggagcttccttggcatggtaagctatcactgtgattggaacttggaagataatgttgaacattactcactgcacatctttactgctataggcctttgcaatgggacttcaggctgttggagcaaatcgtcaccaccttggttctgcaagcgtatatctttcagttggtggcatgctattgtaagttttagaattttatgtcttccgttactttgattctactatctttcaagttatctgaatttagttattaatgtctaggaggctaaactaaatagctgtgactactagttgaataccttaagcaggaggttttatgctcctattggttaagccaatttatgctacatttttgtgccaaatgtgccaatatcaggcttaaaaatatgcaaaattctctacttattgtatctacaaataggattttttttatggtgctctaaatttgttcagaccatccatttcacatcaacccaaaggatatcatttctcttatacctcattagaggtaatagattataagaaggatggaaacaaattattcaacctgatacaaaacattgttgcgtataaattcttccaagaacttgttcaggcaaccttttggttgcatatggtggaacaattctgtgtcaatatatcgatctgacatgtaaatggcactggggatatcagttgcatacatgatattgttctgctttgcagttgtttcattttgattcccctgacttcaccctcagcgttgttgtgcatccttagttattctttatatgaacaatagggctttccatttggtttaatttgtaactagttaaattatgagacagtttacctttatgctgctcttagcagaactctttaattgttcctgacaattatactttactggctcattaagtacaaatgttatggtaagtctaccagtttcttatagttgagtgcttctttttcaggtttgtcttgacattttcgttaggagcaggtccagtgccaggacttcttttgcctgagattttcccaaacaaaatacgggccaaggctatggctctgtgcatgtccgtgcattgggtaaggagttatgtttccatcacaaaaaaaaaaagacactgcatgtctgtgcattggataggagttcatttttcatcctcaaaaaatagacactgcatgtcaaaatgctggcagttagatcaaacagtgaaattcaacgtggaaatttcgttgctgattttcactttttggtttaatatgttaatatttggtattatgtgtctatgcatgtttcctgttccattaggatgtttaaaaatatgttactgctagtatgtaaccatagctgttcacattgttcaatgataaaaggagcctagtgctaaatacctggtgaagaatttgagctgttgtttagtcaggctgagctagaacaggtatattagatcaattcaaaatattcaagctgctaagtcctcaatagcatttccacactgttttaccaaccctggttatggtaatgaagatcagaatttcagaagtatatttgactctgtaagctgcagctttctctgtgtaacaaaatcgtttcccccaatcgccataagataaaaggtactgtatgctgttaataataaatgaataatggggccagatttttaaattagataataaaaaacttagacatttcgatatggttgattatgtaatggtaaatgataagtgtagggttgcaacaataatttggtagggcgcactatttacttgccatggttcattgtagttctaccatgattcgtatcgacgtcttgacatgctatgcttaaatgctgcaggtggtaaatttttttgtcagtttgttgttcttgcggcttctggagcaacttggtccacaagttctgtacacaatgttttcctcagcttgtgtggtagcagcaatatttgtgcggcgccatgtggttgaaacaaagggaaagactttgcaagagatagaagtttcgcttctacaaacacaataaaggtgctaatcttattctgaatctgacaacacaatttggatctgttaatctgtcttatctaatcttgtctttaacgtggcaattttttatttaaaggtggtttgcgagttgcgatgcacatttgaaactcacaagatgaactgcctggatttttagttgatatttcatatggccatgttgatagcaagcttggttttggaacttgatgatttaccatgtggtattcaggattaacaagcagcaatactctttcattgcagatttcatcagcgagtttgaggcaacaacaacctacatgattagcatctgacactatacttgttgctgaatacctgctgggaaagtctaccgtgaaagttgatcactgaaccaagccaacataccacggacaggcagcgccctacactggaccagggaagtacccagacatgcacctgtgaccagtaagggcgacctagctcgtgcataaaccatccatgtagtgaggttgcatctgtatagttggtcttctgctagatgtatctggtaatctgtgttgtcttcatcatttgtcatttgtacactgtcagtatggagaaaaaaagacgggaagtgtggttactgatggccatagtttgaaccgtggatacgttttttttactgtttaaagaggaataacagcactggctctgccagctacatgaaaataaagtgcgttctgcctgagctaattt</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0274900, RefSeq:Os02g0274900